Abstract:
:The major components of vitelline membrane (VM) are ovomucin, VM outer (VMO) I and VMO II. At present, the distribution pattern of maternal cells on the VM has not been described in detail. In this study, the existence and distribution characteristics of maternal cells on VM were observed. There were more than 3.2 × 105 somatic cells on VM, which were uneven distributed. The calcein AM/PI staining of the maternal cells on the VM showed that the cells' viability changed with the freshness of the eggs, and that the maternal cells gradually underwent apoptosis and became degraded. The results of morphology of different tissues indicated that the most of maternal cells on the VM were granulosa cells. Moreover, the karyotype of the cultured granulosa cells, which is the main source of cells on VM, were identified as the normal diploid karyotype of chicken. Furthermore, the VM DNA extracted from chickens and quails, which represent the eggs of different size, was adequate for further genetic analysis. The VM DNA was easily accessible and relatively constant, without cross-contamination. Therefore, the VM DNA could potentially be applied for the molecular traceability between eggs and chickens, and be beneficial in avian ecology research studies.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Li Q,Li W,Li X,Liu L,Zhang Y,Guo Y,Chen X,Xu G,Zheng Jdoi
10.1038/s41598-017-06996-1subject
Has Abstractpub_date
2017-07-26 00:00:00pages
6626issue
1issn
2045-2322pii
10.1038/s41598-017-06996-1journal_volume
7pub_type
杂志文章abstract::2-Bromo-5-(2-bromo-2-nitrovinyl)furan (G1 or Furvina) is an antimicrobial with a direct reactivity against thiol groups. It is active against Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. By reacting with thiol groups it causes direct damage to proteins but, as a result, is very short-living ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36844
更新日期:2016-11-10 00:00:00
abstract::Histone deacetylase inhibition (HDACi) has been suggested as a promising approach to bolster TLR-mediated induction of antimicrobial peptides such as human β-defensin 2 (hBD2). In inflammatory bowel disease (IBD), Crohn's disease (CD) patients display an attenuated expression of hBD2 as compared to ulcerative colitis ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31125-x
更新日期:2018-08-27 00:00:00
abstract::Amorphous thin films from Ge-As-Te system were prepared by pulsed laser deposition to study their intrinsic photostability, morphology, chemical composition, structure and optical properties. Photostability of fabricated layers was studied by spectroscopic ellipsometry within as-deposited as well as relaxed (annealed)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09310
更新日期:2015-03-23 00:00:00
abstract::In this study, ITS, ITS2, matK, rbcL and psbA-trnH in Rehmannia were successfully amplified and sequenced, but some ITS sequences need to be proofread according to ITS2 sequences. Compared with rbcL, matK and psbA-trnH, ITS and ITS2 had higher mutation rate and more information sites, and ITS2 had higher interspecific...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53752-8
更新日期:2019-11-21 00:00:00
abstract::We developed a time-efficient semi-automated axon quantification method using freeware in human cranial nerve sections stained with paraphenylenediamine (PPD). It was used to analyze a total of 1238 facial and masseteric nerve biopsies. The technique was validated by comparing manual and semi-automated quantification ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58917-4
更新日期:2020-02-06 00:00:00
abstract::In this work, we address the ubiquitous phenomenon of Fano resonances in bilayer graphene. We consider that this phenomenon is as exotic as other phenomena in graphene because it can arise without an external extended states source or elaborate nano designs. However, there are not theoretical and/or experimental studi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16838-9
更新日期:2017-12-01 00:00:00
abstract::In the northeastern United States, chronic wasting disease has recently been detected in white-tailed deer (Odocoileus virginianus) populations, and understanding the relationship between landscape configuration and home range may improve disease surveillance and containment efforts. The objectives of our study were t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32937-7
更新日期:2018-10-02 00:00:00
abstract::Gayal (Bos frontalis) is a semi-wild and endangered bovine species that differs from domestic cattle (Bos taurus and Bos indicus), and its genetic background remains unclear. Here, we performed whole-genome sequencing of one Gayal for the first time, with one Red Angus cattle and one Japanese Black cattle as controls....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19787
更新日期:2016-01-25 00:00:00
abstract::Capicua (CIC) has been implicated in pathogenesis of spinocerebellar ataxia type 1 and cancer in mammals; however, the in vivo physiological functions of CIC remain largely unknown. Here we show that Cic hypomorphic (Cic-L(-/-)) mice have impaired bile acid (BA) homeostasis associated with induction of proinflammatory...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08272
更新日期:2015-02-05 00:00:00
abstract::The peptidyl nucleoside blasticidin S (BS) isolated from Streptomyces griseochromogenes was the first non-mercurial fungicide used on a large scale to prevent rice blast. In the biosynthesis of BS, leucylblasticidin S (LBS) was suggested as the penultimate metabolite with 20-fold less inhibitory activity than the fina...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17641
更新日期:2015-12-01 00:00:00
abstract::The role of topical corticosteroids in management of Acanthamoeba keratitis (AK) remains controversial. Using a rabbit AK model, we investigated whether corticosteroid use is a risk factor of AK. Acanthamoeba (1 × 105/ml) was incubated with two densities of P. aeruginosa (PA; high-PA: 1 × 108/ml, low-PA: 3 × 105/ml) b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49128-7
更新日期:2019-09-06 00:00:00
abstract::Airway smooth muscle (ASM) cells exhibit plastic phenotypic behavior marked by reversible modulation and maturation between contractile and proliferative phenotypic states. Integrins are a class of transmembrane proteins that have been implicated as novel therapeutic targets for asthma treatment. We previously showed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46260-2
更新日期:2019-07-09 00:00:00
abstract::Here we quantify the effects of artisanal fisheries on the ecology of a small cetacean, the Ganges River dolphin (Platanista gangetica gangetica, GRD), in a large river system of Nepal. We examine the size-classes of fisheries' catches, behavioural changes in GRD in response to fishing activities, and diel overlap bet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75997-4
更新日期:2020-11-02 00:00:00
abstract::An intensified and continuous West Nile virus (WNV) spread across northern Italy has been observed since 2008, which caused more than one hundred reported human infections until 2016. Veneto is one of the Italian regions where WNV is considered endemic, and the greatest intensity of circulation was observed during 201...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32401-6
更新日期:2018-09-18 00:00:00
abstract::The analysis of cell type-specific activity patterns during behaviors is important for better understanding of how neural circuits generate cognition, but has not been well explored from in vivo neurophysiological datasets. Here, we describe a computational approach to uncover distinct cell subpopulations from in vivo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12474
更新日期:2015-07-27 00:00:00
abstract::Honey bees are under pressure due to abnormal high colony death rates, especially during the winter. The infestation by the Varroa destructor mite and the viruses that this ectoparasite transmits are generally considered as the bees' most important biological threats. Almost all efforts to remedy this dual infection h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71388-x
更新日期:2020-08-31 00:00:00
abstract::Aberrant restoration of AR activity is linked with prostate tumor growth, therapeutic failures and development of castrate-resistant prostate cancer. Understanding the processes leading to AR-reactivation should provide the foundation for novel avenues of drug discovery. A differential gene expression study was conduc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10068-9
更新日期:2017-08-29 00:00:00
abstract::Episodic memories are long lasting and full of detail, yet imperfect and malleable. We quantitatively evaluated recollection of short audiovisual segments from movies as a proxy to real-life memory formation in 161 subjects at 15 minutes up to a year after encoding. Memories were reproducible within and across individ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30175
更新日期:2016-09-30 00:00:00
abstract::Fuel substrate supply and oxidative phosphorylation are key determinants of muscle performance. Numerous studies of mammalian mitochondria are carried out (i) with substrate supply that limits electron flow, and (ii) far below physiological temperature. To analyze potentially implicated biases, we studied mitochondria...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02789-8
更新日期:2017-06-06 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-65026-9
更新日期:2020-05-20 00:00:00
abstract::Superfluidity and superconductivity have been widely studied since the last century in many different contexts ranging from nuclear matter to atomic quantum gases. The rigidity of these systems with respect to external perturbations results in frictionless motion for superfluids and resistance-free electric current fl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08941-8
更新日期:2017-08-22 00:00:00
abstract::Identification of novel brominated contaminants in the environment, especially the derivatives and byproducts of brominated flame retardants (BFRs), has become a wide concern because of their adverse effects on human health. Herein, we qualitatively and quantitatively identified three byproducts of tetrabromobisphenol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11741
更新日期:2015-07-01 00:00:00
abstract::Perivascular spaces (PVSs) in brain have a close relationship with typical neurological diseases. The quantitative studies of PVSs are meaningful but usually difficult, due to their thin and weak signals and also background noise in the 7 T brain magnetic resonance images (MRI). To clearly distinguish the PVSs in the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09336-5
更新日期:2017-08-17 00:00:00
abstract::The most common surgical emergency is suspected acute appendicitis, the lifetime risk of acute appendicitis is estimated to be 8.6% for men and 6.7% for women, with a male to female ratio of 1.4:1; correct diagnosis can be made in 70-80% of patients after the operations about 32% of appendectomies revealed normal appe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58847-1
更新日期:2020-02-06 00:00:00
abstract::Bone is a dynamic organ continuously undergoing shaping, repairing and remodeling. The homeostasis of bone is maintained by the balance between osteoblastic bone formation and osteoclastic bone resorption. Osteoclasts (OCs) are specialized multinucleated cells derived from hematopoietic stem cells (HSCs) or monocytes/...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21499
更新日期:2016-02-09 00:00:00
abstract::It is common to find in the literature different values for the working voltage window (WVW) range for aqueous-based supercapacitors. In many cases, even with the best intentions of the widening the operating voltage window, the measured current using the cyclic voltammetry (CV) technique includes a significant contri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75851-7
更新日期:2020-11-05 00:00:00
abstract::Recently, a new class of nonlinear systems was introduced, in which the self-trapping of fundamental and vortical localized modes in space of dimension D is supported by cubic self-repulsion with a strength growing as a function of the distance from the center, r, at any rate faster that r(D). These systems support ro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09420
更新日期:2015-03-24 00:00:00
abstract::When exploited as cell factories, Saccharomyces cerevisiae cells are exposed to harsh environmental stresses impairing titer, yield and productivity of the fermentative processes. The development of robust strains therefore represents a pivotal challenge for the implementation of cost-effective bioprocesses. Altering ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18318
更新日期:2015-12-14 00:00:00
abstract::Volatile organic compounds (VOCs) represent a broad class of chemicals, many of which can be found in indoor air including residential indoor air. VOCs derive from a variety of sources including cleaning products, cooking practices, fragrances and fresheners, hobbies and at-home work behaviors. This study examined res...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-78756-7
更新日期:2020-12-10 00:00:00
abstract::Dirac-like surface states on surfaces of topological insulators have a chiral spin structure with spin locked to momentum, which is interesting in physics and may also have important applications in spintronics. In this work, by measuring the tunable helicity-dependent photocurrent (HDP), we present an identification ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04889
更新日期:2014-05-08 00:00:00