Abstract：
:The programmed induction of meiotic DNA double-strand breaks (DSBs) by the evolutionarily conserved SPO-11 protein, which is structurally related to archaeal Topo VIA topoisomerases, triggers meiotic recombination. Identification of several meiosis-specific factors that are required for SPO-11-mediated DSB formation raises the question whether SPO-11 alone can cleave DNA. Here, we have developed procedures to express and purify C. elegans SPO-11 in a soluble, untagged, and monodispersed form. Our biochemical and biophysical analyses demonstrate that SPO-11 is monomeric and binds DNA, double-stranded DNA in particular. Importantly, SPO-11 does not exhibit DNA cleavage activity under a wide range of reaction conditions, suggesting that co-factors are needed for DSB induction activity. Our SPO-11 purification system and the findings reported herein should facilitate future mechanistic studies directed at delineating the mechanism of action of the SPO-11 ensemble in meiotic DSB formation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Yeh HY,Lin SW,Wu YC,Chan NL,Chi Pdoi
10.1038/s41598-017-02641-zsubject
Has Abstractpub_date
2017-05-24 00:00:00pages
2370issue
1issn
2045-2322pii
10.1038/s41598-017-02641-zjournal_volume
7pub_type
杂志文章abstract：:Study of knockout (KO) mice has helped understand the link between many genes/proteins and human diseases. Identification of infertile KO mice provides valuable tools to characterize the molecular mechanisms underlying gamete formation. The KIAA0319L gene has been described to have a putative association with dyslexia...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28666-6
更新日期：2018-07-10 00:00:00
abstract：:For the application of formic acid as a liquid organic hydrogen carrier, development of efficient catalysts for dehydrogenation of formic acid is a challenging topic, and most studies have so far focused on the composition of metals and supports, the size effect of metal nanoparticles, and surface chemistry of support...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33502
更新日期：2016-09-26 00:00:00
abstract：:When trying to conceive 1% of couples have recurrent miscarriages, defined as three or more consecutive pregnancy losses. This is not accounted for by the known incidence of chromosomal aneuploidy in miscarriage, and it has been suggested that there is an immunological aetiology. The endometrial mucosa is populated by...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41335
更新日期：2017-01-23 00:00:00
abstract：:The experimental approach for the study of cardiometabolic disorders requires the use of animal models fed with commercial diets whose composition differs notably, even between diets used for control groups. While chow diets are usually made of agricultural by-products, purified low-fat diets (LF) contain a higher per...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59674-0
更新日期：2020-02-19 00:00:00
abstract：:Several anti-cancer therapies target the epidermal growth factor receptor (EGFR). Radionuclide imaging of EGFR expression in tumours may aid in selection of optimal cancer therapy. The 111In-labelled DOTA-conjugated ZEGFR:2377 Affibody molecule was successfully used for imaging of EGFR-expressing xenografts in mice. A...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05700-7
更新日期：2017-07-20 00:00:00
abstract：:It is highly important to understand the heterogeneous wettability properties of porous media for enhanced oil recovery (EOR). However, wettability measurements are still challenging in directly investigating the wettability of porous media. In this paper, we propose a multidimensional nuclear magnetic resonance (NMR)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31803-w
更新日期：2018-09-07 00:00:00
abstract：:Hurricanes are a persistent socio-economic hazard for countries situated in and around the Main Development Region (MDR) of Atlantic tropical cyclones. Climate-model simulations have attributed their interdecadal variability to changes in solar and volcanic activity, Saharan dust flux, anthropogenic greenhouse gas and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12838
更新日期：2015-08-05 00:00:00
abstract：:The realization of reliable quantum channels, able to transfer a quantum state with high fidelity, is a fundamental step in the construction of scalable quantum devices. In this paper we describe a transmission scheme based on the genuinely quantum effect known as Bloch oscillations. The proposed protocol makes it pos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26054
更新日期：2016-05-18 00:00:00
abstract：:Identification of hot spots, a small portion of protein-protein interface residues that contribute the majority of the binding free energy, can provide crucial information for understanding the function of proteins and studying their interactions. Based on our previous method (PredHS), we propose a new computational a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32511-1
更新日期：2018-09-24 00:00:00
abstract：:Addiction to cocaine and other psychostimulants represents a major public health crisis. The development and persistence of addictive behaviors comes from a complex interaction of genes and environment - the precise mechanisms of which remain elusive. In recent years a surge of evidence has suggested that the gut micr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35455
更新日期：2016-10-18 00:00:00
abstract：:Thrombocytopenia is common in patients with dengue virus (DENV) infections. With a focus on understanding the possible mechanism of thrombocytopenia in DENV infections we described a direct correlation between activation and depletion of platelets in patients. Our data showed a sharp decrease in platelet counts at day...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41697
更新日期：2017-01-31 00:00:00
abstract：:Tissue engineering scaffolds are used in conjunction with stem cells for the treatment of various diseases. A number of factors provided by the scaffolds affect the differentiation of stem cells. Mechanical cues that are part of the natural cellular microenvironment can both accelerate the differentiation toward parti...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23214-8
更新日期：2018-03-22 00:00:00
abstract：:Human KHDRBS1/Sam68 is an oncogenic splicing factor involved in signal transduction and pre-mRNA splicing. We explored the molecular mechanism of KHDRBS1 to be a prognostic marker in four different cancers. Within specific cancer, including kidney renal papillary cell carcinoma (KIRP), lung adenocarcinoma (LUAD), acut...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47558-x
更新日期：2019-07-31 00:00:00
abstract：:Several MRI contrast agent clinical formulations are now known to leave deposits of the heavy metal gadolinium in the brain, bones, and other organs of patients. This persistent biological accumulation of gadolinium has been recently recognized as a deleterious outcome in patients administered Gd-based contrast agents...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22511-6
更新日期：2018-03-13 00:00:00
abstract：:Storage of solar radiation is currently accomplished by coupling two separate devices, one that captures and converts the energy into an electrical impulse (a photovoltaic cell) and another that stores this electrical output (a battery or a supercapacitor electrochemical cell). This configuration however has several c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30728-8
更新日期：2018-08-24 00:00:00
abstract：:The movement protein VP37 of broad bean wilt virus 2 (BBWV 2) forms tubules in the plasmodesmata (PD) for the transport of virions between cells. This paper reports a mutual association between the BBWV 2 VP37-tubule complex and PD at the cytological level as determined by transmission electron microscopy. The generat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21552
更新日期：2016-02-23 00:00:00
abstract：:Protein aggregation is a common biological phenomenon, observed in different physiological and pathological conditions. Decreased protein solubility and a tendency to aggregate is also observed during physiological aging but the causes are currently unknown. Herein we performed a biophysical separation of aging-relate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19311
更新日期：2016-01-18 00:00:00
abstract：:The interaction between roots and bacterial communities in halophytic species is poorly understood. Here, we used Jerusalem artichoke cultivar Nanyu 1 (NY-1) to characterise root distribution patterns and determine diversity and abundance of bacteria in the rhizosphere soil under variable salinity. Root growth was not...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20687
更新日期：2016-02-08 00:00:00
abstract：:In the primary somatosensory cortex, large-scale cortical and perceptual changes have been demonstrated following input deprivation. Recently, we found that the cortical and perceptual changes induced by repetitive somatosensory stimulation (RSS) at a finger transfer to the face. However, whether such cross-border cha...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-018-30183-5
更新日期：2018-08-06 00:00:00
abstract：:Soil-transmitted nematodes (STNs), namely hookworms, whipworms, and ascarids, are extremely common parasites, infecting 1-2 billion of the poorest people worldwide. Two benzimidazoles, albendazole and mebendazole, are currently used in STN mass drug administration, with many instances of low/reduced activity reported....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48720-1
更新日期：2019-08-26 00:00:00
abstract：:Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional protein that controls the cellular response to oxidative stress and possesses DNA-repair functions. It has important roles in the progression and outcomes of various diseases; however, its function and therapeutic prospects with respect to kidney injury ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44241-z
更新日期：2019-05-24 00:00:00
abstract：:Demand for clinical decision support systems in medicine and self-diagnostic symptom checkers has substantially increased in recent years. Existing platforms rely on knowledge bases manually compiled through a labor-intensive process or automatically derived using simple pairwise statistics. This study explored an aut...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05778-z
更新日期：2017-07-20 00:00:00
abstract：:Theranostics is the emerging field of medicine that uniquely combines diagnostic techniques and active agents to diagnose and treat medical conditions simultaneously or sequentially. Finding a theranostic agent capable to cure the affected cells and being safe for the healthy ones is the key for successful treatment. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79238-6
更新日期：2020-12-17 00:00:00
abstract：:Tradional antireflection coatings composed of dielectric layers usually require the thickness to be larger than quarter wavelength. Here, we demonstrate that materials with permittivity or permeability dominated by imaginary parts, i.e. lossy or gain media, can realize non-resonant antireflection coatings in deep sub-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28681
更新日期：2016-06-28 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-48627-x
更新日期：2019-08-28 00:00:00
abstract：:Coral reefs have been subject to mass coral bleaching, potentially causing rapid and widespread degradation of ecosystem services that depend on live coral cover, such as fisheries catch. Fisheries species in tropical waters associate with a wide range of habitats, so assessing the dependency of fisheries on coral ree...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74652-2
更新日期：2020-10-20 00:00:00
abstract：:Phthalates are commonly included as ingredients in personal care products such as cosmetics, shampoos and perfumes. Diethyl phthalate (DEP) has been found to be anti-androgenic and linked with adverse reproductive effects on males, but effects on females are poorly understood. We designed an integrative and translatio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63904-w
更新日期：2020-04-27 00:00:00
abstract：:Recent experiments have revealed that some membrane proteins aggregate to form clusters. This type of process has been proven to be dynamic and to be actively maintained by external kinetics. Additionally, this dynamic recruiting is cholesterol- and actin-dependent, suggesting that raft organization and cytoskeleton r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02608
更新日期：2013-01-01 00:00:00
abstract：:Vascular endothelial growth factor receptor 2 (VEGFR2) is a potential cell-type biomarker in clinical diagnoses. Besides, it's the target protein of many tyrosine kinase inhibitors and its expression significantly associates with clinical performance of these inhibitors. VEGFR2 detection provides an early warning for ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03982
更新日期：2014-02-05 00:00:00
abstract：:Vitiligo is characterized by death or functional defects of epidermal melanocytes through still controversial pathogenic process. Previously, we showed that mitochondria-driven pre-senescent phenotype diminishes the capability of vitiligo melanocytes to cope with stressful stimuli. In the current study, we investigate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13961-5
更新日期：2017-10-20 00:00:00
