Abstract:
:Micro-696 (miR-696) has been previously known as an exercise related miRNA, which has a profound role in fatty acid oxidation and mitochondrial biogenesis of skeletal muscle. However, its role in skeletal myoblast proliferation and differentiation is still unclear. In this study, we found that miR-696 expressed highly in skeletal muscle and reduced during C2C12 myoblasts differentiation. MiR-696 overexpression repressed C2C12 myoblast proliferation and myofiber formation, while knockdown of endogenous miR-696 expression showed opposite results. During myogenesis, we observed an inversed expression pattern between miR-696 and CNTFRα in vitro, and demonstrated that miR-696 could specifically target CNTFRα and repress the expression of CNTFRα. Additionally, we further found that knockdown of CNTFRα suppressed the proliferation and differentiation of C2C12 cells. Taking all things together, we propose a novel insight that miR-696 down-regulates C2C12 cell myogenesis by inhibiting CNTFRα expression.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Wang H,Shi L,Liang T,Wang B,Wu W,Su G,Wei J,Li P,Huang Rdoi
10.7150/ijbs.17508subject
Has Abstractpub_date
2017-03-11 00:00:00pages
413-425issue
4issn
1449-2288pii
ijbsv13p0413journal_volume
13pub_type
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