Dedifferentiation of human uterine polyp stem cells into embryo-like cells during inducing pluripotency.


:By introduction of Oct4, Sox2, Klf4 and cMyc, human adult somatic cells can be reprogrammed into embryonic stem cell capable of pluripotent differentiation. In several lines of human endometrial polyp- and cervical polyp-mesenchymal stem cells (EPMSCs and CPMSC), we showed introduction of the four transcription factors led to a dedifferentiation of these cells into early embryo-like cells in three days, ranging from one-cell, two-cell, four-cell embryos, and morula to blastocyst. These early embryo-like cells resembled human early embryo derived from in vitro fertilization (IVF) in morphology, and hatching activity. These cells also expressed hypoblast (GATA4) and trophoblast (Cdx2) markers. After culturing the embryo-like cells for one month, the induced pluripotency stem cells (iPSC) could be formed (proved by pluripotency gene expression, by in vitro and in vivo differentiation). C/EBPα expression was also increased in uterine polyps. In contrast, MSCs derived from normal endometrium could not be induced to dedifferentiation to such early embryo-like cells. We conclude that EPMSCs and CPMSCs could be dedifferentiated to early embryo-like cells by the iPSC cocktail. This suggests that polyps of the organ derived from Mullerian duct may harbor epigenetic markers making them vulnerable to reprogramming to the earliest developmental stage. This study provides a simple model to derive early human embryo-like cells by in vitro.


Int J Biol Sci


Ding DC,Chu TY,Liu HW




Has Abstract


2018-09-07 00:00:00












  • The gain of function of p53 mutant p53S in promoting tumorigenesis by cross-talking with H-RasV12.

    abstract::The loss of wild type p53 tumor suppressive function and oncogenic gain-of-function of p53 mutants have been showing important implications in tumorigenesis. The p53(N236S) (p53(N239S) in human, p53S) mutation has been shown to lose wild type p53 function by yeast assay. However, its gain of function is still not clea...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Jia S,Zhao L,Tang W,Luo Y

    更新日期:2012-01-01 00:00:00

  • Phosphoinositide specific phospholipase Cγ1 inhibition-driven autophagy caused cell death in human lung adenocarcinoma A549 cells in vivo and in vitro.

    abstract::Our previous studies indicated that phosphoinositide specific phospholipase Cγ1 (PLCγ1) was involved in autophagy induction in colon and hepatic carcinoma cells. However, whether and how PLCγ1 regulation in human lung adenocarcinoma is linked to autophagy remains unclear. Here, we assessed the protein expression of PL...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Lu X,Fu H,Chen R,Wang Y,Zhan Y,Song G,Hu T,Xia C,Tian X,Zhang B

    更新日期:2020-02-21 00:00:00

  • Coenzyme Q10 protects astrocytes from ROS-induced damage through inhibition of mitochondria-mediated cell death pathway.

    abstract::Coenzyme Q10 (CoQ10) acts by scavenging reactive oxygen species to protect neuronal cells against oxidative stress in neurodegenerative diseases. The present study was designed to examine whether CoQ10 was capable of protecting astrocytes from reactive oxygen species (ROS) mediated damage. For this purpose, ultraviole...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Jing L,He MT,Chang Y,Mehta SL,He QP,Zhang JZ,Li PA

    更新日期:2015-01-01 00:00:00

  • Interleukin-11 promotes the progress of gastric carcinoma via abnormally expressed versican.

    abstract::Versican, a ubiquitous component of the extracellular matrix (ECM), accumulates both in tumor stroma and cancer cells and is highly regulated by various cytokines. The aberrant expression of versican and its isoforms is known to modulate cell proliferation, differentiation, and migration, all of which are features of ...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Zhang Z,Zhang J,Miao L,Liu K,Yang S,Pan C,Jiao B

    更新日期:2012-01-01 00:00:00

  • Chronic Osteomyelitis Increases the Incidence of Type 2 Diabetes in Humans and Mice.

    abstract::Background: To compare the risk of type 2 diabetes (T2DM) between patients with and without chronic osteomyelitis (COM), both in humans and in mice, and to explore risk factors in COM patients who developed T2DM. Methods: One hundred seven patients with COM and 114 patients without COM were consecutively enrolled and ...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Wang Y,Wang J,Deng Z,Jin J,Jiang H,Meng J,Xu H,Zhao J,Sun G,Qian H

    更新日期:2017-09-05 00:00:00

  • Farrerol Ameliorates APAP-induced Hepatotoxicity via Activation of Nrf2 and Autophagy.

    abstract::Farrerol has been shown to have antioxidative potential via Nrf2 activation, which in turn is involved in the prevention of hepatotoxicity. The purpose of the current study was to explore the protective effect of farrerol against acetaminophen-induced hepatotoxicity and its underlying mechanisms. Mice were used to eva...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Wang L,Wei W,Xiao Q,Yang H,Ci X

    更新日期:2019-01-29 00:00:00

  • The small molecule PSSM0332 disassociates the CRL4ADCAF8 E3 ligase complex to decrease the ubiquitination of NcoR1 and inhibit the inflammatory response in a mouse sepsis-induced myocardial dysfunction model.

    abstract::Sepsis-induced myocardial dysfunction (SIMD) is a life-threatening complication caused by inflammation, but how it is initiated is still unclear. Several studies have shown that extracellular high mobility group box 1 (HMGB1), an important cytokine triggering inflammation, is overexpressed during the pathogenesis of S...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Peng Q,Xu H,Xiao M,Wang L

    更新日期:2020-09-19 00:00:00

  • Important functional roles of basigin in thymocyte development and T cell activation.

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    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Yao H,Teng Y,Sun Q,Xu J,Chen YT,Hou N,Cheng X,Yang X,Chen ZN

    更新日期:2013-12-13 00:00:00

  • Gamma-secretase Inhibitor Prevents Proliferation and Migration of Ductus Arteriosus Smooth Muscle Cells through the Notch3-HES1/2/5 Pathway.

    abstract::Patent ductus arteriosus (PDA) can cause morbidity and mortality in neonates. Vascular remodeling, characterized by proliferation and migration of smooth muscle cells (SMCs), is an essential process for postnatal DA closure. Notch signaling is an important mediator of vascular remodelling but its role in DA is unkonwn...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Wu JR,Yeh JL,Liou SF,Dai ZK,Wu BN,Hsu JH

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  • Current Status of Therapeutic Approaches against Peripheral Nerve Injuries: A Detailed Story from Injury to Recovery.

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    journal_title:International journal of biological sciences

    pub_type: 杂志文章,评审


    authors: Hussain G,Wang J,Rasul A,Anwar H,Qasim M,Zafar S,Aziz N,Razzaq A,Hussain R,de Aguilar JG,Sun T

    更新日期:2020-01-01 00:00:00

  • Adapted Resistance to the Knockdown Effect of shRNA-Derived Srsf3 siRNAs in Mouse Littermates.

    abstract::Gene silencing techniques are widely used to control gene expression and have potential for RNAi-based therapeutics. In this report, transgenic mouse lines were created for conditional knockdown of Srsf3 (SRp20) expression in liver and mammary gland tissues by expressing Srsf3-specific shRNAs driven by a U6 promoter. ...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Ajiro M,Jia R,Wang RH,Deng CX,Zheng ZM

    更新日期:2015-09-03 00:00:00

  • The role of TGF-β/SMAD4 signaling in cancer.

    abstract::Transforming growth factor β (TGF-β) signaling pathway plays important roles in many biological processes, including cell growth, differentiation, apoptosis, migration, as well as cancer initiation and progression. SMAD4, which serves as the central mediator of TGF-β signaling, is specifically inactivated in over half...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章,评审


    authors: Zhao M,Mishra L,Deng CX

    更新日期:2018-01-12 00:00:00

  • Thermal injury-plus-sepsis contributes to a substantial deletion of intestinal mesenteric lymph node CD4 T cell via apoptosis.

    abstract::Thermal injury (TI) with septic complications continues to be a serious clinical problem. One of the main concerns in such patients is immunosuppression related to functional derangements in intestinal CD4+ T lymphocytes. Extensive previous studies in thermal injury/septic patients and animal models of thermal injury/...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Fazal N,Al-Ghoul WM

    更新日期:2007-09-12 00:00:00

  • Murine Wee1 plays a critical role in cell cycle regulation and pre-implantation stages of embryonic development.

    abstract::Wee1 kinase regulates the G2/M cell cycle checkpoint by phosphorylating and inactivating the mitotic cyclin-dependent kinase 1 (Cdk1). Loss of Wee1 in many systems, including yeast and drosophila, leads to premature mitotic entry. However, the developmental role of Wee1 in mammals remains unclear. In this study, we es...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Tominaga Y,Li C,Wang RH,Deng CX

    更新日期:2006-01-01 00:00:00

  • MiR-216a-5p inhibits tumorigenesis in Pancreatic Cancer by targeting TPT1/mTORC1 and is mediated by LINC01133.

    abstract::MiR-216a-5p has opposite effects on tumorigenesis and progression in the context of different tumors, acting as either a tumor suppressor or an oncogene. However, the expression and function of miR-216a-5p in pancreatic cancer (PC) is not well characterized. In this study, we found miR-216a-5p was significantly downre...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Zhang J,Gao S,Zhang Y,Yi H,Xu M,Xu J,Liu H,Ding Z,He H,Wang H,Hao Z,Sun L,Liu Y,Wei F

    更新日期:2020-07-19 00:00:00

  • Amphioxus: a peaceful anchovy fillet to illuminate Chordate Evolution (I).

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    journal_title:International journal of biological sciences

    pub_type: 社论


    authors: Garcia-Fernàndez J

    更新日期:2006-01-01 00:00:00

  • Effects of hypothyroidism on expression of CRMP2B and ARPC5 during development of the rat frontal cortex.

    abstract::Congenital hypothyroidism (CH) can lead to irreversible central nervous system (CNS) damage. However, the pathogenesis of the developmental brain disorders caused by CH has not been completely elucidated. ARPC5 and CRMP2 are closely associated with neurite outgrowth in brain development. Thus, the aim of the present s...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Liu CR,Miao J,Zhang YL,Liu YM,Yu BG

    更新日期:2013-01-01 00:00:00

  • miR-1204 promotes hepatocellular carcinoma progression through activating MAPK and c-Jun/AP1 signaling by targeting ZNF418.

    abstract::Emerging evidence has indicated that abnormal microRNAs (miRNAs) participated in carcinogenesis and tumor progression in hepatocellular carcinoma (HCC). Better understanding the association between miRNAs and HCC may contribute to discover novel therapeutic approaches for diagnosis and treatments. In the current study...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Wang L,Sun L,Wang Y,Yao B,Liu R,Chen T,Tu K,Liu Q,Liu Z

    更新日期:2019-06-02 00:00:00

  • Capsaicin mediates cell cycle arrest and apoptosis in human colon cancer cells via stabilizing and activating p53.

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    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Jin J,Lin G,Huang H,Xu D,Yu H,Ma X,Zhu L,Ma D,Jiang H

    更新日期:2014-02-21 00:00:00

  • Targeting the Endocytic Pathway and Autophagy Process as a Novel Therapeutic Strategy in COVID-19.

    abstract::Coronaviruses (CoVs) are a group of enveloped, single-stranded positive genomic RNA viruses and some of them are known to cause severe respiratory diseases in human, including Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (MERS) and the ongoing coronavirus disease-19 (COVID-19). One key el...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章,评审


    authors: Yang N,Shen HM

    更新日期:2020-03-15 00:00:00

  • Genome-wide association analysis of meat quality traits in a porcine Large White × Minzhu intercross population.

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    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Luo W,Cheng D,Chen S,Wang L,Li Y,Ma X,Song X,Liu X,Li W,Liang J,Yan H,Zhao K,Wang C,Wang L,Zhang L

    更新日期:2012-01-01 00:00:00

  • Pathological Characterization Of IFNAR(-/-) Mice Infected With Bluetongue Virus Serotype 4.

    abstract::Bluetongue virus (BTV) replicates in lymphoid tissues where infected mononuclear leukocytes secrete proinflammatory and vasoactive mediators that can contribute to bluetongue (BT) pathogenesis. Using the well-characterized IFNAR(-/-) mice animal model, we have now studied the histopathology and dynamics of leukocyte p...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Marín-López A,Bermúdez R,Calvo-Pinilla E,Moreno S,Brun A,Ortego J

    更新日期:2016-11-24 00:00:00

  • Oncogenic Protein Kinase D3 Regulating Networks in Invasive Breast Cancer.

    abstract::Protein Kinase D3 (PRKD3) functions as an important oncogenic driver in invasive breast cancer, which is the leading cause of women mortality. However, PRKD3 regulating network is largely unknown. In this study, we systematically explored PRKD3 regulating networks via investigating phosphoproteome, interactome and tra...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Liu Y,Li J,Zhang J,Yu Z,Yu S,Wu L,Wang Y,Gong X,Wu C,Cai X,Mo L,Wang M,Gu J,Chen L

    更新日期:2017-05-16 00:00:00

  • Discovery of candidate genes for muscle traits based on GWAS supported by eQTL-analysis.

    abstract::Biochemical and biophysical processes that take place in muscle under relaxed and stressed conditions depend on the abundance and activity of gene products of metabolic and structural pathways. In livestock at post-mortem, these muscle properties determine aspects of meat quality and are measurable. The conversion of ...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Ponsuksili S,Murani E,Trakooljul N,Schwerin M,Wimmers K

    更新日期:2014-03-10 00:00:00

  • Transarterial Chemoembolization: A Favorable Postoperative Management to Improve Prognosis of Hepatitis B Virus-associated Intrahepatic Cholangiocarcinoma after Surgical Resection.

    abstract::Background: There is no information regarding transarterial chemoembolization (TACE) as a postoperative management after hepatic resection for patients with hepatitis B virus (HBV)-associated intrahepatic cholangiocarcinoma (ICC). Methods: Forty-two patients with pathological confirmation of HBV-associated ICC were en...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Jeong S,Zheng B,Wang J,Chi J,Tong Y,Xia L,Xu N,Zhang J,Kong X,Gu J,Xia Q

    更新日期:2017-09-21 00:00:00

  • Mast cell-nerve cell interaction at acupoint: modeling mechanotransduction pathway induced by acupuncture.

    abstract::Mast cells are found abundant at sites of acupoints. Nerve cells share perivascular localization with mast cells. Acupuncture (mechanical stimuli) can activate mast cells to release adenosine triphosphate (ATP) which can activate nerve cells and modulates pain-processing pathways in response to acupuncture. In this pa...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Yao W,Yang H,Yin N,Ding G

    更新日期:2014-04-26 00:00:00

  • Incorporation of DDR2 clusters into collagen matrix via integrin-dependent posterior remnant tethering.

    abstract::Cell-matrix interactions play critical roles in cell adhesion, tissue remodeling and cancer metastasis. Discoidin domain receptor 2 (DDR2) is a collagen receptor belonging to receptor tyrosine kinase (RTK) family. It is a powerful regulator of collagen deposition in the extracellular matrix (ECM). Although the oligome...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Li T,Liu J,Cai H,Wang B,Feng Y,Liu J

    更新日期:2018-04-30 00:00:00

  • Interleukin-6 receptor blockade selectively reduces IL-21 production by CD4 T cells and IgG4 autoantibodies in rheumatoid arthritis.

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    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Carbone G,Wilson A,Diehl SA,Bunn J,Cooper SM,Rincon M

    更新日期:2013-01-01 00:00:00

  • Enhancement of larval RNAi efficiency by over-expressing Argonaute2 in Bombyx mori.

    abstract::RNA interference has been described as a powerful genetic tool for gene functional analysis and a promising approach for pest management. However, RNAi efficiency varies significantly among insect species due to distinct RNAi machineries. Lepidopteran insects include a large number of pests as well as model insects, s...

    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Li Z,Zeng B,Ling L,Xu J,You L,Aslam AF,Tan A,Huang Y

    更新日期:2015-01-05 00:00:00

  • Spexin alleviates insulin resistance and inhibits hepatic gluconeogenesis via the FoxO1/PGC-1α pathway in high-fat-diet-induced rats and insulin resistant cells.

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    journal_title:International journal of biological sciences

    pub_type: 杂志文章


    authors: Gu L,Ding X,Wang Y,Gu M,Zhang J,Yan S,Li N,Song Z,Yin J,Lu L,Peng Y

    更新日期:2019-11-01 00:00:00