Abstract:
:Different stimuli often activate the same intracellular signaling molecules but trigger distinct cell responses. We explored whether or not MAPK signaling induced by macrophage colony-stimulating factor (M-CSF), which is responsible for osteoclast proliferation, differs from that induced by receptor activator of NF-κB ligand (RANKL), which is essential for inducing osteoclast differentiation. The activation of MAPKs by M-CSF or RANKL differed in terms of the extent and duration of ERK, p38, and JNK phosphorylation as well as the isoform specificity of JNK phosphorylation. In particular, RANKL induced a second wave of MAPK activation coincident with the onset of osteoclast differentiation, whereas M-CSF triggered only a monophasic response. M-CSF was also able to trigger a full MAPK response on restimulation of cells earlier than was RANKL, representing that MAPK resensitization by M-CSF differs from that by RANKL. Furthermore, the adapter protein TRAF6 recruitment to the cytoplasmic tail of RANK in a submembrane compartment is specifically required for RANKL-induced activation of p38 MAPK, expression of osteoclastogenic transcription factors, and osteoclast differentiation, indicating that the switch from proliferation to differentiation in osteoclast precursors is dependent on p38 activation via the RANKL-RANK-TRAF6 axis. Our results suggest that selective control of MAPK signaling induced by M-CSF and by RANKL mediates the proliferation versus differentiation decision in osteoclast precursors.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Lee K,Chung YH,Ahn H,Kim H,Rho J,Jeong Ddoi
10.7150/ijbs.13814subject
Has Abstractpub_date
2016-01-01 00:00:00pages
235-45issue
2issn
1449-2288pii
ijbsv12p0235journal_volume
12pub_type
杂志文章abstract::Interleukin-8 (IL-8) is a common inflammatory factor, which involves in various non-specific pathological processes of inflammation. It has been found that increased endothelial permeability accompanied with high expression of IL-8 at site of injured endothelium and atherosclerotic plaque at early stages, suggesting t...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.6996
更新日期:2013-09-23 00:00:00
abstract::Recently, the first clinical trials on Bioartificial Livers (BALs) loaded with a proliferative human hepatocyte cell source have started. There are two cell lines that are currently in an advanced state of BAL development; HepaRG and HepG2/C3A. In this study we aimed to compare both cell lines on applicability in BALs...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.15165
更新日期:2016-06-24 00:00:00
abstract::Signal transducer and activator of transcription 3 (STAT3) is a transcription factor that is activated by many cytokines and growth factors and plays a key role in cell survival, proliferation, and differentiation. STAT3 activation is detected virtually in all rodent models of liver injury and in human liver diseases....
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.7.536
更新日期:2011-04-27 00:00:00
abstract::By introduction of Oct4, Sox2, Klf4 and cMyc, human adult somatic cells can be reprogrammed into embryonic stem cell capable of pluripotent differentiation. In several lines of human endometrial polyp- and cervical polyp-mesenchymal stem cells (EPMSCs and CPMSC), we showed introduction of the four transcription factor...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.23401
更新日期:2018-09-07 00:00:00
abstract::Pathogens have developed strategies to modify Dendritic Cells (DCs) phenotypes and impair their functions in order to create a safer environment for their survival. DCs responses to helminths and their derivatives vary among different studies. Here we show that excretory/secretory products of the cestode Taenia crassi...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.1391
更新日期:2011-01-01 00:00:00
abstract::Many organisms in extremely cold environments such as the Antarctic Pole have evolved antifreeze molecules to prevent ice formation. There are four types of antifreeze proteins (AFPs). Type-IV antifreeze proteins (AFP4s) are present also in certain temperate and even tropical fish, which has raised a question as to wh...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.9126
更新日期:2014-06-24 00:00:00
abstract::Rheumatoid arthritis (RA) is characterized by synovial lining hyperplasia, which involves abnormal growth of fibroblast-like synoviocytes (FLSs). This study aimed to investigate the function and molecular mechanism of peptidylarginine deiminase type 4 (PADI4) in FLSs isolated from RA patients (RA-FLSs). FLSs were isol...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.16879
更新日期:2017-02-25 00:00:00
abstract::Cell-matrix interactions play critical roles in cell adhesion, tissue remodeling and cancer metastasis. Discoidin domain receptor 2 (DDR2) is a collagen receptor belonging to receptor tyrosine kinase (RTK) family. It is a powerful regulator of collagen deposition in the extracellular matrix (ECM). Although the oligome...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.24765
更新日期:2018-04-30 00:00:00
abstract::Metastasis is a complicated, multistep process that is responsible for over 90% of cancer-related death. Metastatic disease or the movement of cancer cells from one site to another requires dramatic remodeling of the cytoskeleton. The regulation of cancer cell migration is determined not only by biochemical factors in...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.44943
更新日期:2020-04-27 00:00:00
abstract::Pacemaker activity of the sinoatrial node has been studied extensively in various animal species, but is virtually unexplored in man. As such, it is unknown whether the fast sodium current (I(Na)) plays a role in the pacemaker activity of the human sinoatrial node. Recently, we had the unique opportunity to perform pa...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5.201
更新日期:2009-01-01 00:00:00
abstract::The Zucker diabetic fatty (ZDF) rat is a model of type II diabetes and metabolic syndrome based on impaired glucose tolerance caused by the inherited insulin-resistance gene. The ZDF rat exhibits progressive nephropathy; however, the detailed mechanisms have remained unclear. This study was performed to examine the po...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.3.40
更新日期:2006-11-01 00:00:00
abstract::Despite years of research into bone formation, the mechanisms by which transcription factors specify growth plate development and trabecular bone formation remain unclear and the role of hypertrophic chondrocytes in trabeculae morphogenesis is controversial. To study the role of Core binding factor beta (Cbfβ) in post...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.8521
更新日期:2014-07-29 00:00:00
abstract::Mast cells are found abundant at sites of acupoints. Nerve cells share perivascular localization with mast cells. Acupuncture (mechanical stimuli) can activate mast cells to release adenosine triphosphate (ATP) which can activate nerve cells and modulates pain-processing pathways in response to acupuncture. In this pa...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.8631
更新日期:2014-04-26 00:00:00
abstract::Obesity and type 2 diabetes mellitus are complicated metabolic diseases that affect multiple organs and are characterized by hyperglycaemia. Currently, stable and effective treatments for obesity and type 2 diabetes mellitus are not available. Therefore, the mechanisms leading to obesity and diabetes and more effectiv...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.27173
更新日期:2018-08-06 00:00:00
abstract::Chronic back pain is a global health problem affecting millions of people worldwide and carries significant economic and social morbidities. Intervertebral disc damage and degeneration is a major cause of back pain, characterised by histological and biochemical changes that have been well documented in animal models. ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5.388
更新日期:2009-06-03 00:00:00
abstract::Farrerol has been shown to have antioxidative potential via Nrf2 activation, which in turn is involved in the prevention of hepatotoxicity. The purpose of the current study was to explore the protective effect of farrerol against acetaminophen-induced hepatotoxicity and its underlying mechanisms. Mice were used to eva...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.30677
更新日期:2019-01-29 00:00:00
abstract::Colon cancer is a malignant type of cancer with high prevalence and is one of the primary causes of cancer-related deaths. Oxaliplatin plays a significant role in the treatment of cancer, but the application of oxaliplatin is restricted due to its toxic side effects and drug resistance in clinical practice. Therefore,...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.35265
更新日期:2019-06-04 00:00:00
abstract::Osteosarcoma (OS) is considered the most common type of primary malignant bone tumor, which has an urgent need for more effective treatment. Recently, chitinase 3 like 1 (Chi3L1) expression has been found in a variety of cancer cells. However it is not known whether Chi3L1 regulates the STAT3 pathway in OS cells. Here...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.37781
更新日期:2020-01-01 00:00:00
abstract::Background and aims: Dysfunction of the immune regulatory system plays a role in the pathogenesis of allergic rhinitis (AR). The underlying mechanism needs to be further investigated. Published data indicate that soluble CD83 (sCD83) has immune regulatory activities. This study aims to investigate the role of sCD83 in...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.38722
更新日期:2020-01-01 00:00:00
abstract::Carbon monoxide (CO) is a vasoactive molecule that is generated by vascular cells as a byproduct of heme catabolism and it plays an important physiological role in circulation system. In order to investigate whether exogenous CO can mediate the growth and proliferation of vascular cells, in this study, we used 250 par...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.4664
更新日期:2012-01-01 00:00:00
abstract::N6-methyladenosine (m6A) is identified as the most prevalent and abundant internal RNA modification, especially within eukaryotic mRNAs, which has attracted much attention in recent years since its importance for regulating gene expression and deciding cell fate. m6A modification is installed by RNA methyltransferases...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.45231
更新日期:2020-04-15 00:00:00
abstract::Proteomic profiling of the pectoralis muscle of Thai indigenous chickens during growth period was analyzed using two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/MS). A total of 259, 161, 120 and 107 protein spots were found to be ex...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5.679
更新日期:2009-10-28 00:00:00
abstract:: Objectives: Skeletal development is a complex process. Little is known about the different response of limb or spine growth plate chondrocytes (LGP or SGP) to the estrogen level and the role of estrogen receptor (ER) during postnatal stage. Methods...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.17696
更新日期:2017-01-11 00:00:00
abstract::Glucocorticoid medication is one of the most common causes of atraumatic osteonecrosis of the femoral head (ONFH), and vitamin K2 (VK2) has been shown to play an important and beneficial role in bone metabolism. In this study, we hypothesized that VK2 could decrease the incidence of glucocorticoid-induced ONFH in a ra...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.13269
更新日期:2016-01-28 00:00:00
abstract::Non-small cell lung cancer (NSCLC) with epidermal growth factor receptor (EGFR) wild-type is intrinsic resistance to EGFR-tyrosine kinase inhibitors (TKIs). In this study, we assessed whether the combination of bisdemethoxycurcumin (BDMC) and icotinib could surmount primary EGFR-TKI resistance in NSCLC cells and inves...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.40042
更新日期:2020-03-05 00:00:00
abstract::Cell density has profound impacts on the cell culture practices of human pluripotent stem cells. The regulation of cell growth, cell death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act at this stage. We use a chemically defined medium to s...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.24681
更新日期:2018-04-05 00:00:00
abstract::Basigin is a highly glycosylated transmembrane protein that is expressed in a broad range of tissues and is involved in a number of physiological and pathological processes. However, the in vivo role of basigin remains unknown. To better understand the physiological and pathological functions of basigin in vivo, we ge...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.6818
更新日期:2013-12-13 00:00:00
abstract::Tumor-associated macrophages (TAMs) are closely correlated with tumor occurrence, invasion, and metastasis. However, factors affecting the biological functions of TAMs in colorectal cancer (CRC) are incompletely understood. Here, we found that Wnt5a was mainly expressed on TAMs of tumor stroma but not on CRC cells. Su...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.40535
更新日期:2020-02-04 00:00:00
abstract::MicroRNAs (miRNAs) are a class of small, non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition in plants and animals. At present there is relatively little information regarding the role of miRNAs in the response to drought stress in maize. In this study, ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.11619
更新日期:2015-05-22 00:00:00
abstract::MiR-216a-5p has opposite effects on tumorigenesis and progression in the context of different tumors, acting as either a tumor suppressor or an oncogene. However, the expression and function of miR-216a-5p in pancreatic cancer (PC) is not well characterized. In this study, we found miR-216a-5p was significantly downre...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.46822
更新日期:2020-07-19 00:00:00