Abstract:
:Carbon monoxide (CO) is a vasoactive molecule that is generated by vascular cells as a byproduct of heme catabolism and it plays an important physiological role in circulation system. In order to investigate whether exogenous CO can mediate the growth and proliferation of vascular cells, in this study, we used 250 parts per million (ppm) of CO to treat human umbilical artery smooth muscle cell (hUASMC) and human umbilical vein endothelial cell (HuVEC) and further evaluated the growth and apoptosis status of SMC and HuVEC. After SMC and HuVEC were exposed to CO for 7-day, the growth of SMC and HuVEC was significantly inhibited by CO in vitro on day 5 of CO exposure. And CO blocked cell cycle progress of SMC and HuVEC, more SMC and HuVEC stagnated at G0/G1 phase by flow cytometric analysis. Moreover, CO treatment inhibited SMC and HuVEC apoptosis caused by hydrogen peroxide through decreasing caspase 3 and 9 activities. To confirm the molecular mechanism of CO effect on SMC and HuVEC growth, we compared the gene expression profile in SMC and CO-treated SMC, HuVEC and CO-treated HuVEC. By microarray analysis, we found the expression level of some genes which are related to cell cycle regulation, cell growth and proliferation, and apoptosis were changed during CO exposure. We further identified that the down-regulated CDK2 contributed to arresting cell growth and the down-regulated Caspase 3 (CASP3) and Caspase 9 (CASP9) were associated with the inhibition of cell apoptosis. Therefore, CO exerts a certain growth arrest on SMC and HuVEC by inhibiting cell cycle transition from G0/G1 phase to S phase and has regulatory effect on cell apoptosis by regulating the expression of apoptosis-associated genes.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Li Y,Wang H,Yang B,Yang J,Ruan X,Yang Y,Wakeland EK,Li Q,Fang Xdoi
10.7150/ijbs.4664subject
Has Abstractpub_date
2012-01-01 00:00:00pages
1431-46issue
10issn
1449-2288pii
ijbsv08p1431journal_volume
8pub_type
杂志文章abstract::Genetic variant T329S in flavin-containing monooxygenase 3 (FMO3) impairs trimethylamine (TMA) metabolism in birds. The TMA metabolism that under complex genetic and dietary regulation, closely linked to cardiovascular disease risk. We determined whether the genetic defects in TMA metabolism may change other metabolic...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.16472
更新日期:2016-10-25 00:00:00
abstract::The heterotrimeric G-protein alpha subunit has long been considered a bimodal, GTP-hydrolyzing switch controlling the duration of signal transduction by seven-transmembrane domain (7TM) cell-surface receptors. In 1996, we and others identified a superfamily of "regulator of G-protein signaling" (RGS) proteins that acc...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.1.51
更新日期:2005-01-01 00:00:00
abstract::Objective: Recent studies demonstrate circulating serum spexin levels are reduced in obesity or type 2 diabetes mellitus (T2DM) patients and may play a role in glucose metabolism. The mechanism underlying is not known. In this study, we explore whether spexin has a role in insulin resistance and hepatic glucose metabo...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.31781
更新日期:2019-11-01 00:00:00
abstract::Although it has been widely applied in identification of genes responsible for biomedically, economically, or even evolutionarily important complex and quantitative traits, traditional candidate gene approach is largely limited by its reliance on the priori knowledge about the physiological, biochemical or functional ...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.3.420
更新日期:2007-10-25 00:00:00
abstract::Wee1 kinase regulates the G2/M cell cycle checkpoint by phosphorylating and inactivating the mitotic cyclin-dependent kinase 1 (Cdk1). Loss of Wee1 in many systems, including yeast and drosophila, leads to premature mitotic entry. However, the developmental role of Wee1 in mammals remains unclear. In this study, we es...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.2.161
更新日期:2006-01-01 00:00:00
abstract::Emerging evidence has indicated that abnormal microRNAs (miRNAs) participated in carcinogenesis and tumor progression in hepatocellular carcinoma (HCC). Better understanding the association between miRNAs and HCC may contribute to discover novel therapeutic approaches for diagnosis and treatments. In the current study...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.33658
更新日期:2019-06-02 00:00:00
abstract::Spinal cord injury (SCI) induces the disruption of the blood-spinal cord barrier (BSCB) which leads to infiltration of blood cells, an inflammatory response, and neuronal cell death, resulting spinal cord secondary damage. Retinoic acid (RA) has a neuroprotective effect in both ischemic brain injury and SCI, however t...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.13229
更新日期:2016-01-01 00:00:00
abstract::Although the fibroblast growth factor (FGF) signaling axis plays important roles in cell survival, proliferation, and differentiation, the molecular mechanism underlying how the FGF elicits these diverse regulatory signals is not well understood. By using the Frs2α null mouse embryonic fibroblast (MEF) in conjunction ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.1114
更新日期:2011-01-01 00:00:00
abstract::Transforming growth factor-beta (TGF-beta) family members exert their function via specific type I and type II serine/threonine kinase receptors and intracellular Smad transcription factors, including the common mediator Smad4. The dual effects of TGF-beta signaling on tumor initiation and progression are cell-specifi...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.6.1
更新日期:2010-01-01 00:00:00
abstract::Transforming growth factor β (TGF-β) signaling pathway plays important roles in many biological processes, including cell growth, differentiation, apoptosis, migration, as well as cancer initiation and progression. SMAD4, which serves as the central mediator of TGF-β signaling, is specifically inactivated in over half...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.23230
更新日期:2018-01-12 00:00:00
abstract::Dyschromatosis universalis hereditaria (DUH) is an autosomal dominant pigmentary genodermatosis characterized by the presence of patches of hyperpigmentation and hypopigmented macules distributed over the body, with most cases reported in Asia. DUH is a heterogeneous disease and a small portion of patients carry the A...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.38415
更新日期:2020-02-10 00:00:00
abstract::The development of alternative energy technology is critically important because of the rising prices of crude oil, security issues regarding the oil supply, and environmental issues such as global warming and air pollution. Bioconversion of biomass has significant advantages over other alternative energy strategies b...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.5.578
更新日期:2009-09-04 00:00:00
abstract::A LIM-homeobox gene, AmphiLim1/5, from the Florida amphioxus (Branchiostoma floridae) encodes a protein that phylogenetic analysis positions at the base of a clade comprising vertebrate Lim1 and Lim5. Amphioxus AmphiLim1/5 is expressed in domains that are a composite of those of vertebrate Lim1 and Lim5, which evident...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.2.110
更新日期:2006-01-01 00:00:00
abstract::Peripheral nerve injury is a complex condition with a variety of signs and symptoms such as numbness, tingling, jabbing, throbbing, burning or sharp pain. Peripheral nerves are fragile in nature and can easily get damaged due to acute compression or trauma which may lead to the sensory and motor functions deficits and...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.35653
更新日期:2020-01-01 00:00:00
abstract::Cell-matrix interactions play critical roles in cell adhesion, tissue remodeling and cancer metastasis. Discoidin domain receptor 2 (DDR2) is a collagen receptor belonging to receptor tyrosine kinase (RTK) family. It is a powerful regulator of collagen deposition in the extracellular matrix (ECM). Although the oligome...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.24765
更新日期:2018-04-30 00:00:00
abstract::Despite years of research into bone formation, the mechanisms by which transcription factors specify growth plate development and trabecular bone formation remain unclear and the role of hypertrophic chondrocytes in trabeculae morphogenesis is controversial. To study the role of Core binding factor beta (Cbfβ) in post...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.8521
更新日期:2014-07-29 00:00:00
abstract::CKLF-like MARVEL transmembrane domain-containing member (CMTM) is a new gene family first cloned and reported in 2001. The CMTM family consists of nine members including CKLF and CMTM1-CMTM8, which are located on different chromosomes. Besides exhibiting extensive chemotactic activity, the CMTM family plays an importa...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.33733
更新日期:2019-09-07 00:00:00
abstract::Melatonin is present in mammalian follicular fluid and plays an important role in regulating steroidogenesis in follicular cells. In this study, we report the effect of melatonin on steroidogenesis in the theca interna (TI) in small bovine follicles and theca cells (TCs) cultured in vitro. Treatment with melatonin sig...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.27912
更新日期:2019-01-01 00:00:00
abstract::Background and aim: Mesenchymal stem cells (MSCs) are capable of impacting tumor progression but its role in tumor stroma remodeling still remains unclear. This present study was aimed to evaluate the potential function of MSCs on tumor stroma remodeling using rabbits VX2 bladder tumor model. Methods: The VX2 bladder ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.25200
更新日期:2018-06-03 00:00:00
abstract::MicroRNAs (miRNAs) are a class of small, non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition in plants and animals. At present there is relatively little information regarding the role of miRNAs in the response to drought stress in maize. In this study, ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.11619
更新日期:2015-05-22 00:00:00
abstract::Non-coding RNAs (ncRNAs) are transcribed from genome but not translated into proteins. Many ncRNAs are key regulators of plants growth and development, metabolism and stress tolerance. In order to make the web-based ncRNA resources for plant science research be more easily accessible and understandable, we made a comp...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.24593
更新日期:2018-05-22 00:00:00
abstract::During the immune response, neutrophils display localized mechanical events by interacting with their environment through the micro-vascular transit, trans-endothelial, and trans-epithelial migration. Nano-mechanical studies of human neutrophils on localized nano-domains could provide the essential information for und...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.102
更新日期:2011-01-20 00:00:00
abstract::RNA interference has been described as a powerful genetic tool for gene functional analysis and a promising approach for pest management. However, RNAi efficiency varies significantly among insect species due to distinct RNAi machineries. Lepidopteran insects include a large number of pests as well as model insects, s...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.10235
更新日期:2015-01-05 00:00:00
abstract::Background: To compare the risk of type 2 diabetes (T2DM) between patients with and without chronic osteomyelitis (COM), both in humans and in mice, and to explore risk factors in COM patients who developed T2DM. Methods: One hundred seven patients with COM and 114 patients without COM were consecutively enrolled and ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.21379
更新日期:2017-09-05 00:00:00
abstract::Thymosin alpha 1 (Tα1) has immunomodulatory and anti-tumor effects in patients and has been commercialized in worldwide. An innovative technique is therefore impending to achieve high-yield expression and purification of Tα1 to meet the increasing requirements for clinical applications. Tα1 can enhance T cells, dendri...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.618
更新日期:2011-01-01 00:00:00
abstract::Pacemaker activity of the sinoatrial node has been studied extensively in various animal species, but is virtually unexplored in man. As such, it is unknown whether the fast sodium current (I(Na)) plays a role in the pacemaker activity of the human sinoatrial node. Recently, we had the unique opportunity to perform pa...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5.201
更新日期:2009-01-01 00:00:00
abstract::It is a basic task in high-throughput gene expression profiling studies to identify differentially expressed genes (DEGs) between two phenotypes. But the weakly differential expression signals between two phenotypes are hardly detectable with limited sample sizes. To solve this problem, many researchers tried to combi...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.24548
更新日期:2018-05-22 00:00:00
abstract::To investigate the effects of Genistein on the osteogenic related gene expression profiles during osteoblastic differentiation of human bone marrow mesenchymal stem cell (hBMSC) cultures, the hBMSCs were cultured under osteogenic differentiation medium with the addition of Genistein (10(-8)∼10(-5) M) for 12 days. The ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7367
更新日期:2013-11-21 00:00:00
abstract::The objective of this work was to evaluate the bioavailability of heme iron added to biscuit filling. It comprised two stages: first, the development of the heme iron enriched biscuit filling; second, the evaluation of the bioavailability of the mineral in fattening piglets. Two groups were selected randomly and fed: ...
journal_title:International journal of biological sciences
pub_type: 杂志文章,随机对照试验
doi:10.7150/ijbs.4.58
更新日期:2008-02-18 00:00:00
abstract::Recently, the first clinical trials on Bioartificial Livers (BALs) loaded with a proliferative human hepatocyte cell source have started. There are two cell lines that are currently in an advanced state of BAL development; HepaRG and HepG2/C3A. In this study we aimed to compare both cell lines on applicability in BALs...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.15165
更新日期:2016-06-24 00:00:00