Abstract:
:Infectious HCV carrying reporter genes have further applications in understanding the HCV life cycle including replication, viral assembly and release. In this study, a full-length 3039bp LacZ gene was inserted into the derivative of JFH1-AM120 to develop an additional reporter virus. The results showed that the recombinant reporter virus JFH1-AM120-LacZ can replicate and produce lower titers of infectious virus. However, insertion of the LacZ gene in the C-terminal region of the NS5A in HCV JFH1-AM120-LacZ decreased viral replication and dramatically impaired the production of infectious viral particles. Moreover, the JFH1-AM120-LacZ reporter virus lost the LacZ gene after serial passage. Nevertheless, the JFH1-AM120-LacZ reporter virus displayed the entire life cycle of HCV, from replication to production of infectious virus, in Huh7.5 cells. This study demonstrates that the NS5A region of HCV JFH1-AM120 has the capacity to accommodate large foreign genes up to 3,039 bp and suggests that other relatively large gene inserts can be accommodated at this site.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Wang Q,Hagedorn C,Liu Sdoi
10.7150/ijbs.27411subject
Has Abstractpub_date
2018-07-13 00:00:00pages
1211-1220issue
10issn
1449-2288pii
ijbsv14p1211journal_volume
14pub_type
杂志文章abstract::Metastasis is a complicated, multistep process that is responsible for over 90% of cancer-related death. Metastatic disease or the movement of cancer cells from one site to another requires dramatic remodeling of the cytoskeleton. The regulation of cancer cell migration is determined not only by biochemical factors in...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.44943
更新日期:2020-04-27 00:00:00
abstract::To investigate the effects of Genistein on the osteogenic related gene expression profiles during osteoblastic differentiation of human bone marrow mesenchymal stem cell (hBMSC) cultures, the hBMSCs were cultured under osteogenic differentiation medium with the addition of Genistein (10(-8)∼10(-5) M) for 12 days. The ...
journal_title:International journal of biological sciences
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journal_title:International journal of biological sciences
pub_type: 杂志文章
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journal_title:International journal of biological sciences
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journal_title:International journal of biological sciences
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journal_title:International journal of biological sciences
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doi:10.7150/ijbs.4742
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.17948
更新日期:2017-10-17 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.28609
更新日期:2019-09-07 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.39895
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.40042
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.740
更新日期:2011-01-01 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.11883
更新日期:2015-04-27 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.10174
更新日期:2015-01-01 00:00:00
abstract::Dyschromatosis universalis hereditaria (DUH) is an autosomal dominant pigmentary genodermatosis characterized by the presence of patches of hyperpigmentation and hypopigmented macules distributed over the body, with most cases reported in Asia. DUH is a heterogeneous disease and a small portion of patients carry the A...
journal_title:International journal of biological sciences
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doi:10.7150/ijbs.38415
更新日期:2020-02-10 00:00:00
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journal_title:International journal of biological sciences
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更新日期:2015-05-22 00:00:00
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journal_title:International journal of biological sciences
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journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.25712
更新日期:2018-04-30 00:00:00
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journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
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journal_title:International journal of biological sciences
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journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
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更新日期:2018-05-22 00:00:00
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journal_title:International journal of biological sciences
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更新日期:2013-06-16 00:00:00
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journal_title:International journal of biological sciences
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更新日期:2020-07-19 00:00:00
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journal_title:International journal of biological sciences
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journal_title:International journal of biological sciences
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journal_title:International journal of biological sciences
pub_type: 杂志文章
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更新日期:2019-03-10 00:00:00