Abstract:
:Genuine racial differences in prostate cancer (PCa) biology have been considered among the potential reasons to explain PCa disparities. There is no animal model to represent all aspects of human PCa and, more specifically, to be used for PCa disparity research. The lack of a spontaneously transformed in vitro cell-based model system has been a significant impediment to investigating and understanding potential molecular mechanisms, and the hormonal, genetic, and epigenetic factors underlying the biological and clinical aggressiveness of PCa in African American (AA) men. In this study, we established and characterized the E006AA-hT cell line as a highly tumorigenic subline of the previously characterized primary AA-PCa cell line, E006AA. Extensive characterization of the E006AA-hT cell line was accomplished using cytodifferentiation and prostate-specific markers, spectral karyotyping, cell line authentication assays, cell proliferation and migration assays, and in vitro tumorigenesis assays. Spectral karyotyping of E006AA-hT showed a hypertriploid chromosome complement and shared cytogenetic changes similar to its parental cells such as diploid X, absence of Y-chromosomes, numerical gains in chromosomes 5,6,8,10,17,20,21, and marker chromosomes of unknown origin. In addition, E006AA-hT also presented numerous clonal and structural aberrations such as insertion, deletion, duplication, and translocations in chromosomes 1-5, 8, 9, 11, 13, 14, 17, and 18. The E006AA-hT cell line was shown to be highly tumorigenic and produced tumors at an accelerated growth rate in both athymic nude and triple-deficient SCID mice. Silencing the mutated androgen receptor (AR-599 Ser>Gly) did not affect proliferation (loss-of-function), but decreased migration (gain-of-function) in E006AA-hT and its parental cell type. These data support that AR-point mutations may lead simultaneously to different "loss-of-function" and "gain-of-function" phenotypes in PCa cells. E006AA-Par and its subline as the only available spontaneously transformed low- and highly-tumorigenic primary AA-PCa cell lines could be used for basic and translational research aimed in supporting prostate cancer disparity research.
journal_name
Int J Biol Scijournal_title
International journal of biological sciencesauthors
Koochekpour S,Willard SS,Shourideh M,Ali S,Liu C,Azabdaftari G,Saleem M,Attwood Kdoi
10.7150/ijbs.9406subject
Has Abstractpub_date
2014-07-26 00:00:00pages
834-45issue
8issn
1449-2288pii
ijbsv10p0834journal_volume
10pub_type
杂志文章abstract::Embryonic stem (ES) cells have immortality for self-renewal and pluripotency. Differentiated human cells undergo replicative senescence. In human, the telomerase reverse transcriptase (Tert), namely the catalytic subunit of telomerase, exhibits differential expression to regulate telomerase activity governing cellular...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.426
更新日期:2011-04-15 00:00:00
abstract::Carbon monoxide (CO) is a vasoactive molecule that is generated by vascular cells as a byproduct of heme catabolism and it plays an important physiological role in circulation system. In order to investigate whether exogenous CO can mediate the growth and proliferation of vascular cells, in this study, we used 250 par...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.4664
更新日期:2012-01-01 00:00:00
abstract::The p38 MAPK kinase pathway is activated in response to a wide range of cellular stress stimuli and cytokines. Our understanding of the important functions of p38 MAPK in the process of differentiation and cell death has grown considerably in the recent years and is now relatively established. Here we discuss the role...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.5.44
更新日期:2009-01-01 00:00:00
abstract::A recent breakthrough showing that direct trans-differentiation of chondrocytes into bone cells commonly occurs during endochondral bone formation in the growth plate, articular cartilage, and mandibular condylar cartilage suggests that chondrogenesis and osteogenesis are likely one continuous biological process inste...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.23165
更新日期:2018-01-01 00:00:00
abstract::Capsaicin is the major pungent ingredient in red peppers which is world widely consumed. Except its potent pain relieving efficacy as reported, capsaicin also exerted its antitumor activity in several tumor models. Here, we reported that capsaicin had a profound anti-proliferative effect on human colon cancer cells vi...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7730
更新日期:2014-02-21 00:00:00
abstract::Proteomic profiling of the pectoralis muscle of Thai indigenous chickens during growth period was analyzed using two-dimensional gel electrophoresis (2-DE) and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/MS). A total of 259, 161, 120 and 107 protein spots were found to be ex...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5.679
更新日期:2009-10-28 00:00:00
abstract::Transforming growth factor β (TGF-β) signaling pathway plays important roles in many biological processes, including cell growth, differentiation, apoptosis, migration, as well as cancer initiation and progression. SMAD4, which serves as the central mediator of TGF-β signaling, is specifically inactivated in over half...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.23230
更新日期:2018-01-12 00:00:00
abstract::Type 2 diabetes mellitus is characterized by insulin resistance. However, the complete molecular mechanism remains unclear. In this study, zebrafish were fed a long-term high-fat diet to induce type 2 diabetes, which resulted in a higher body weight, body mass index, more lipid vacuoles in liver, increased insulin tra...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.19249
更新日期:2017-07-18 00:00:00
abstract::Two assumptions underlie current models of the geographical ranges of perennial plant species: 1. current ranges are in equilibrium with the prevailing climate, and 2. changes are attributable to changes in macroclimatic factors, including tolerance of winter cold, the duration of the growing season, and water stress ...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.4866
更新日期:2012-01-01 00:00:00
abstract::Background: The preoperative controlling nutritional status (CONUT) score and circulating tumour cell (CTC) status are associated with poor prognosis of colorectal cancer (CRC). The aim of the present study is to determine whether the combination of CONUT and CTC (CONUT-CTC) could better predict the prognosis of CRC p...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.33671
更新日期:2019-05-12 00:00:00
abstract::Peripheral nerve injury is a complex condition with a variety of signs and symptoms such as numbness, tingling, jabbing, throbbing, burning or sharp pain. Peripheral nerves are fragile in nature and can easily get damaged due to acute compression or trauma which may lead to the sensory and motor functions deficits and...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.35653
更新日期:2020-01-01 00:00:00
abstract::Retrospective and prospective studies show that lean mass or strength is positively associated with metabolic health. Mice deficient in myostatin, a growth factor that negatively regulates skeletal muscle mass, have increased muscle and body weights and are resistant to diet-induced obesity. Their leanness is often at...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.13525
更新日期:2016-04-01 00:00:00
abstract::The Zucker diabetic fatty (ZDF) rat is a model of type II diabetes and metabolic syndrome based on impaired glucose tolerance caused by the inherited insulin-resistance gene. The ZDF rat exhibits progressive nephropathy; however, the detailed mechanisms have remained unclear. This study was performed to examine the po...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.3.40
更新日期:2006-11-01 00:00:00
abstract::Statins, known as HMG-CoA reductase (HMGCR) inhibitors, have primarily been utilized for metabolic and angiographic medical applications because of their cholesterol-lowering effects. Similar to other drugs, statins may also induce a series of potential side effects. Statins inhibit the HMGCR (rate-limiting enzyme) ac...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.42965
更新日期:2020-08-13 00:00:00
abstract::Pancreatic cancer (PC) is one of the most lethal diseases, characterized by early metastasis and high mortality. Subunits of the SWI/SNF complex have been identified in many studies as the regulators of tumor progression, but the role of SMARCAD1, one member of the SWI/SNF family, in pancreatic cancer has not been elu...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.29562
更新日期:2019-01-01 00:00:00
abstract::Solid tumors consist of various types of stromal cells in addition to cancer cells. Cancer-associated fibroblasts (CAFs) are a major component of the tumor stroma and play an essential role in tumor progression and metastasis in a variety of malignancies, including gastric cancer. However, the effects of CAFs on gastr...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.33750
更新日期:2019-08-22 00:00:00
abstract::Angiogenesis plays an important role in myocardial repair after myocardial infarction (MI). Cardiac micro-vascular endothelial cells (CMECs) are important participants in myocardial angiogenesis processes. Recent studies have revealed that Nuclear factor-erythroid 2-related factor 2 (Nrf2), a master transcription fact...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5887
更新日期:2013-07-07 00:00:00
abstract::Breast cancer (BC) is the most common cause of death in women throughout the world. Although microRNAs (miRNAs) have been identified as novel regulators in carcinogenesis, there are still abundant hidden treasure needed to be excavated. In the present study, we found that miR-519d expression was remarkably decreased i...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.22849
更新日期:2018-02-09 00:00:00
abstract::Pathogens have developed strategies to modify Dendritic Cells (DCs) phenotypes and impair their functions in order to create a safer environment for their survival. DCs responses to helminths and their derivatives vary among different studies. Here we show that excretory/secretory products of the cestode Taenia crassi...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.1391
更新日期:2011-01-01 00:00:00
abstract::MicroRNAs (miRNAs) are a class of small, non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition in plants and animals. At present there is relatively little information regarding the role of miRNAs in the response to drought stress in maize. In this study, ...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.11619
更新日期:2015-05-22 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a highly heterogeneous disease, which makes the prognostic prediction challenging. Ferroptosis, an iron-dependent form of regulated cell death, can be induced by sorafenib. However, the prognostic value of ferroptosis-related genes in HCC remains to be further elucidated. In this stud...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.45050
更新日期:2020-07-06 00:00:00
abstract::Farrerol has been shown to have antioxidative potential via Nrf2 activation, which in turn is involved in the prevention of hepatotoxicity. The purpose of the current study was to explore the protective effect of farrerol against acetaminophen-induced hepatotoxicity and its underlying mechanisms. Mice were used to eva...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.30677
更新日期:2019-01-29 00:00:00
abstract:: Objectives: Skeletal development is a complex process. Little is known about the different response of limb or spine growth plate chondrocytes (LGP or SGP) to the estrogen level and the role of estrogen receptor (ER) during postnatal stage. Methods...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.17696
更新日期:2017-01-11 00:00:00
abstract::During the immune response, neutrophils display localized mechanical events by interacting with their environment through the micro-vascular transit, trans-endothelial, and trans-epithelial migration. Nano-mechanical studies of human neutrophils on localized nano-domains could provide the essential information for und...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.7.102
更新日期:2011-01-20 00:00:00
abstract::Pathological cardiac hypertrophy is the response of heart to various biomechanical and physiopathological stimuli, such as aging, myocardial ischemia and hypertension. However, a long-term exposure to the stress makes heart progress to heart failure. Autophagy is a dynamic self-degradative process necessary for the ma...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.11883
更新日期:2015-04-27 00:00:00
abstract::The evading apoptosis of tumor cells may result in chemotherapy resistance. Therefore, investigating what molecular events contribute to drug-induced apoptosis, and how tumors evade apoptotic death, provides a paradigm to explain the relationship between cancer genetics and treatment sensitivity. In this study, we foc...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.36829
更新日期:2019-10-15 00:00:00
abstract:BACKGROUND & OBJECTIVE:The incidence of non-small cell lung cancer (NSCLC) has been rising over the past several decades. Despite various therapeutic regimens and modern diagnostic techniques are developed, NSCLC still have an extremely poor prognosis due to drug resistance. Therefore, it is critical to find a novel pr...
journal_title:International journal of biological sciences
pub_type: 杂志文章,评审
doi:10.7150/ijbs.22243
更新日期:2018-03-10 00:00:00
abstract::Protein Kinase D3 (PRKD3) functions as an important oncogenic driver in invasive breast cancer, which is the leading cause of women mortality. However, PRKD3 regulating network is largely unknown. In this study, we systematically explored PRKD3 regulating networks via investigating phosphoproteome, interactome and tra...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.18472
更新日期:2017-05-16 00:00:00
abstract::Ovarian cancer is the fifth common cause of death in woman worldwide. The tripartite motif-containing (TRIM) proteins consist of more than 70 known protein members. Studies have showed that TRIM proteins are involved in cancer and play important roles in cancer cell proliferation, migration, adhesion and metastasis. R...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.28757
更新日期:2018-11-03 00:00:00
abstract::Congenital hypothyroidism (CH) can lead to irreversible central nervous system (CNS) damage. However, the pathogenesis of the developmental brain disorders caused by CH has not been completely elucidated. ARPC5 and CRMP2 are closely associated with neurite outgrowth in brain development. Thus, the aim of the present s...
journal_title:International journal of biological sciences
pub_type: 杂志文章
doi:10.7150/ijbs.5646
更新日期:2013-01-01 00:00:00