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Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine.

Abstract：

PURPOSE:Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). METHODOLOGY:An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNA-Seq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. RESULTS:Vaccination significantly reduced fever and interferon-gamma (IFNγ) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcine-derived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. CONCLUSION:Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits.

journal_name

J Med Microbiol

journal_title

Journal of medical microbiology

authors

Bearson BL,Bearson SMD,Brunelle BW,Bayles DO,Lee IS,Kich JD

doi

10.1099/jmm.0.000482

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

651-661

issue

eissn

0022-2615

issn

1473-5644

journal_volume

pub_type

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Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine.