Abstract：
:The distribution and composition of endogenous lipids in articular cartilage and transport of exogenous fatty acids have been investigated on a microscopic scale in fresh bovine articular cartilage. To investigate the distribution and composition of the endogenous lipids, hyperspectral Raman maps were taken of chondrocytes and their surrounding matrix in both the deep and superficial zones. These revealed differences in both lipid distribution and composition between the two zones. Extracellular lipid was observed surrounding the cells in the superficial zone but not in the deep zone. Additionally, intracellular lipid droplets were observed that were larger and more numerous in the deep zone (P = 0.01). The extracellular lipid was primarily free saturated fatty acid, whereas the cellular lipid droplets contained triglycerides with unsaturated fatty acid chains. Fatty acid uptake and transport were investigated by incubating cartilage samples in Dulbecco's modified Eagle's medium containing fluorescently labelled palmitate for a range of times and temperatures. After incubation, the palmitate distribution was imaged using confocal microscopy. Palmitate accumulated preferentially in the territorial matrix only in the superficial zone where the concentration was up to 100-fold greater than that in the bulk matrix (P = 0.001). Palmitate uptake by the chondrocytes in both zones showed differential temperature sensitivity (P = 0.05), which would support the idea that cells take up palmitate by both active and passive mechanisms. The study reveals large differences between chondrocytes in the superficial and deep zones in their lipid content, in their extracellular lipid environment and in their access to exogenous fatty acids.
journal_name
J Anatjournal_title
Journal of anatomyauthors
Mansfield JC,Winlove CPdoi
10.1111/joa.12624subject
Has Abstractpub_date
2017-07-01 00:00:00pages
156-166issue
1eissn
0021-8782issn
1469-7580journal_volume
231pub_type
杂志文章abstract：:The number of large neurons in the lateral vestibular nucleus of the mouse brain decreases in number after 25 months of age, from 488 at 25 months to 298 at 31 months. The number of small neurons in the nucleus remains constant up to 31 months of age. Large neurons accumulate lipofuscin from 25 months and their mean n...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Primates have shoulders adapted to a wide range of locomotor functions from terrestrial pronograde quadrupedalism to highly arboreal suspensory behaviours. The shape of the scapula tightly follows these functional differences. Previous analyses of primate postcrania, including the scapula, indicate that quadrupedal mo...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2006.00639.x
更新日期：2006-11-01 00:00:00
abstract：:Degeneration induced by intracranial section of the ophthalmic branch of the trigeminal nerve was used as a marker to trace the ocular passage and termination of fibres in ocular filtration angle structures of 3 cynomolgus monkeys. Fine supraciliary nerves branched from precorneal nerves as they entered the sclera fro...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:The distribution of mRNAs and antigens of tissue type (t) and urokinase type (u) plasminogen activators (PA) plus their corresponding inhibitors, type-1 (PAI-1) and type-2 (PAI-2) were studied in human and rhesus monkey placentae by in situ hybridisation and immunocytochemistry. Specific monkey cRNA and antibodies aga...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1999.19420183.x
更新日期：1999-02-01 00:00:00
abstract：:Axonal elements of the carotid baroreceptor zone of nine domestic fowls were examined with the electron microscope after either distal vagal ganglionectomy or midcervical vagotomy. The bare baroreceptor endings and the central axon of the encapsulated baroreceptor endings degenerated within 5 to 15 days after ganglion...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:This study presents, for the first time, sequences of five beta-keratin cDNAs from turtle epidermis obtained by means of 5'- and 3'-rapid amplification of cDNA ends (RACE) analyses. The deduced amino acid sequences correspond to distinct glycine-proline-serine-tyrosine rich proteins containing 122-174 amino acids. In ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2008.01030.x
更新日期：2009-02-01 00:00:00
abstract：:We previously identified a pericellular matrix keratan sulphate/chondroitin sulphate proteoglycan present on the surface of human embryonal carcinoma stem cells, cells whose differentiation mimics early development. Antibodies reactive with various epitopes on this molecule define a cluster of differentiation markers ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2002.00034.x
更新日期：2002-03-01 00:00:00
abstract：:Enzymatic degradation followed by lectin application was carried out on specimens of the rabbit oviduct. The sequential application of the glycosidases (neuraminidase, alpha-L-fucosidase, beta-galactosidase and alpha-mannosidase) and lectins (peanut, winged pea, wheat germ and soybean) seem promising as an effective m...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:In embryology, the infracardiac bursa (ICB) is a well-known derivative separated from the omental bursa. During surgeries around the esophagogastric junction (EGJ), surgeons often encounter a closed space considered to be equivalent to the ICB, but the macroscopic anatomy in adults is hardly known. This study aimed to...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12989
更新日期：2019-07-01 00:00:00
abstract：:The elastic cartilage of the rat epiglottis was studied with a high-resolution SEM technique. The chondrocytes were found to be anchored in their lacunae by fibrils running in from the territorial matrix. This matrix exhibited a dense network of fibrils arranged tangentially around the lacunar cavity. The fibrils of t...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
abstract：:The permeability of the skin of fetal rats between 14 and 21 days of intrauterine life was investigated by injecting lanthanum nitrate solution subepidermally and by exposing the external surface of other fetuses to the same solution. Up to the age of 18 days, the epidermis was freely permeable from the basal surface ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:Apoptosis is an essential physiological process in embryonic development. In the developing eye of vertebrates, three periods of developmental apoptosis can be distinguished: early, intermediate and later. Within the apoptosis pathway, caspases play a crucial role. It has also been shown that HSP110 may have a potenti...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2007.00719.x
更新日期：2007-05-01 00:00:00
abstract：:The production of echolocation calls in bats along with forces produced by contraction of thoracic musculature used in flight presumably puts relatively high mechanical loads on the lower respiratory tract (LRT). Thus, there are likely adaptations to prevent collapse or distortion of the bronchial tree and trachea dur...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12575
更新日期：2017-04-01 00:00:00
abstract：:A monoclonal antibody SBU-3 raised against an antigen expressed by the binucleate cells in the trophoblast of the sheep placentome crossreacts with an antigen expressed by binucleate cells in the placenta of the deer and the cow. This monoclonal antibody has been used to study the distribution of this subpopulation of...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:Rodents, together with bats, are among the ecologically most diverse and most speciose groups of mammals. Moreover, rodents show elaborate specializations of the feeding apparatus in response to the predominantly fore-aft movements of the lower jaw. The Laotian rock rat Laonastes aenigmamus was recently discovered and...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2012.01519.x
更新日期：2012-08-01 00:00:00
abstract：:Videodigitised outlines of C6, C7, T1 and T2 vertebrae from 23 mice carrying the mutant gene undulated (un/un) and normal littermates (+/un) have been examined by Fourier analysis. There were large differences in shape between C6 vertebrae, smaller but significant differences in C7 and insignificant differences betwee...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:It has been shown that gibbons are able to brachiate with very low mechanical costs. The conversion of muscle activity into smooth, purposeful movement of the limb depends on the morphometry of muscles and their mechanical action on the skeleton. Despite the gibbon's reputation for excellence in brachiation, little in...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2009.01109.x
更新日期：2009-09-01 00:00:00
abstract：:The study of inborn genetic errors can lend insight into mechanisms of normal human development and congenital malformations. Here, we present the first detailed comparison of cranial and neuro pathology in two exceedingly rare human individuals with cyclopia and alobar holoprosencephaly (HPE) in the presence and abse...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12343
更新日期：2015-09-01 00:00:00
abstract：:During development, muscle growth is usually finely adapted to meet functional demands in daily activities. However, how muscle geometry changes in typically developing children and how these changes are related to functional and mechanical properties is largely unknown. In rodents, longitudinal growth of the pennate ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2011.01402.x
更新日期：2011-09-01 00:00:00
abstract：:Glutamate kills neurons by excitotoxicity, which is caused by sustained activation of glutamate receptors. In recent years, it has been shown that glutamate can also be toxic to white matter oligodendrocytes and to myelin by this mechanism. In particular, glutamate receptor-mediated injury to these cells can be trigge...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1111/j.1469-7580.2007.00733.x
更新日期：2007-06-01 00:00:00
abstract：:The lumbar muscular system, in particular the lumbar multifidus muscle (LM) and the erector spinae muscle (ES), plays an important role in stabilizing and mobilizing the lumbar spine. Based on the topography, the lumbar paraspinal muscles can be classified into local and global muscles. LM is part of the local system,...
journal_title：Journal of anatomy
pub_type： 临床试验,杂志文章
doi：10.1111/joa.12867
更新日期：2018-10-01 00:00:00
abstract：:The developing human brain can compensate for pre- and perinatally acquired focal lesions more effectively than the adult brain. The mechanisms by which this effective reorganization is achieved vary considerably between different functional systems, reflecting differences in the normal maturation of these systems. In...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1111/j.1469-7580.2010.01262.x
更新日期：2010-10-01 00:00:00
abstract：:We sought to study the effect of synovectomy on the surface morphology of articular cartilages of the rabbit knee using scanning electron microscopy (SEM). Fifteen rabbits were surgically synovectomised and allowed to regenerate their synovia during time intervals ranging from 3 to 44 wk. Cartilage specimens were shav...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1998.19230343.x
更新日期：1998-04-01 00:00:00
abstract：:Overactivity of the hypothalamic-pituitary-adrenal (HPA) axis characterized by hypercortisolism, adrenal hyperplasia and abnormalities in negative feedback is the most consistently described biological abnormality in melancholic depression. Corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) are the m...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1111/j.1469-7580.2005.00443.x
更新日期：2005-09-01 00:00:00
abstract：:The extracellular matrix (ECM) of connective tissues enables linking to other tissues, and plays a key role in force transmission and tissue structure maintenance in tendons, ligaments, bone and muscle. ECM turnover is influenced by physical activity, and both collagen synthesis and metalloprotease activity increase w...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1111/j.1469-7580.2006.00549.x
更新日期：2006-04-01 00:00:00
abstract：:A quantitative and morphological study was made of the corpus callosum in children to assess possible sex differences. The shape of the splenium of the corpus callosum was observed to vary between bulbous and cylindrical types with respect to the body of the callosum. The variation was not gender-specific and covariat...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:We present a simple and quick system for accurately scoring the developmental progress of mouse embryos harvested on embryonic day 14 (E14.5). Based solely on the external appearance of the maturing forelimb, we provide a convenient way to distinguish six developmental sub-stages. Using a variety of objective morphome...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12590
更新日期：2017-05-01 00:00:00
abstract：:Proper assessment of outcome in clinical trials of neural transplantation requires both biochemical and imaging indices of graft survival, and behavioural and physiological indices of graft function. For transplantation in Huntington's disease, a variety of ligands that are selective for striatal degeneration and graf...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1046/j.1469-7580.2000.19640597.x
更新日期：2000-05-01 00:00:00
abstract：:The sacroiliac joint (SIJ) is a well-known source of low back and pelvic pain, of increasing interest for both conservative and surgical treatment. Alterations in the kinematics of the pelvis have been hypothesized as a major cause of SIJ-related pain. However, definitions of both the range and the extent of physiolog...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12924
更新日期：2019-03-01 00:00:00
abstract：:Pulmonary complications are frequently observed after transthoracic oesophagectomy. These complications may be reduced by sparing the vagus nerve branches to the lung. However, current descriptions of the regional anatomy are insufficient. Therefore, we aimed to provide a highly detailed description of the course of t...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12366
更新日期：2015-10-01 00:00:00
