Abstract：
:This study presents, for the first time, sequences of five beta-keratin cDNAs from turtle epidermis obtained by means of 5'- and 3'-rapid amplification of cDNA ends (RACE) analyses. The deduced amino acid sequences correspond to distinct glycine-proline-serine-tyrosine rich proteins containing 122-174 amino acids. In situ hybridization shows that beta-keratin mRNAs are expressed in cells of the differentiating beta-layers of the shell scutes. Southern blotting analysis reveals that turtle beta-keratins belong to a well-conserved multigene family. This result was confirmed by the amplification and sequencing of 13 genomic fragments corresponding to beta-keratin genes. Like snake, crocodile and avian beta-keratin genes, turtle beta-keratins contain an intron that interrupts the 5'-untranslated region. The length of the intron is variable, ranging from 0.35 to 1.00 kb. One of the sequences obtained from genomic amplifications corresponds to one of the five sequences obtained from cDNA cloning; thus, sequences of a total of 17 turtle beta-keratins were determined in the present study. The predicted molecular weight of the 17 different deduced proteins range from 11.9 to 17.0 kDa with a predicted isoelectric point of 6.8-8.4; therefore, they are neutral to basic proteins. A central region rich in proline and with beta-strand conformation shows high conservation with other reptilian and avian beta-keratins, and it is likely involved in their polymerization. Glycine repeat regions, often containing tyrosine, are localized toward the C-terminus. Phylogenetic analysis shows that turtle beta-keratins are more similar to crocodilian and avian beta-keratins than to those of lizards and snakes.
journal_name
J Anatjournal_title
Journal of anatomyauthors
Dalla Valle L,Nardi A,Toni M,Emera D,Alibardi Ldoi
10.1111/j.1469-7580.2008.01030.xsubject
Has Abstractpub_date
2009-02-01 00:00:00pages
284-300issue
2eissn
0021-8782issn
1469-7580pii
JOA1030journal_volume
214pub_type
杂志文章abstract：:The number of large neurons in the lateral vestibular nucleus of the mouse brain decreases in number after 25 months of age, from 488 at 25 months to 298 at 31 months. The number of small neurons in the nucleus remains constant up to 31 months of age. Large neurons accumulate lipofuscin from 25 months and their mean n...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:The periodontal ligament was examined by light microscopy at 3 different levels (apical, intermediate, coronal) on the 4 root sides of 43 extracted teeth. Epithelial rests were localised and serial LM and EM sections showed a close apposition (up to 0.03 microns) between Ruffini-like and free nerve endings and the bas...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:The aim of the study was to evaluate the distribution of apoptosis in the medullary nuclei of infants and adults who died of hypoxic-ischaemic injury. Apoptosis was studied by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) in brainstems from 22 adults (7 subjects who died of opiate into...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2007.00842.x
更新日期：2008-02-01 00:00:00
abstract：:An electron microscopic study of myelination was carried out in the anterior limb of the anterior commissure of the mouse brain. The total number of axons increased from 48 700 at 17 days post-conception to 286 500 at 11 days postnatum. The first evidence of myelination was the presence of a few promyelin fibres at 8 ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1975-02-01 00:00:00
abstract：:The mammalian cochlea is a remarkable sensory organ, capable of perceiving sound over a range of 10(12) in pressure, and discriminating both infrasonic and ultrasonic frequencies in different species. The sensory hair cells of the mammalian cochlea are exquisitely sensitive, responding to atomic-level deflections at s...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1111/joa.12314
更新日期：2016-02-01 00:00:00
abstract：:In the spinotrapezius muscle of the rat and the mouse approximately equal proportions of A,B and C fibres are present. The spinotrapezius therefore contains a lower proportion of B fibres than are known to be present in soleus, but a higher proportion than in tibialis anterior. These results are consistent with the fu...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：:A quantitative analysis of the motor innervation of intrafusal muscle fibres is described, based on teased silver-impregnated spindles of the tenuissimus muscle of the cat. Included in the analysis are the number and distribution of intrafusal branches of both skeletofusimotor (beta) and purely fusimotor (gamma) axons...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1994-08-01 00:00:00
abstract：:Myocardial hyperplasia is generally considered to occur only during fetal development. However, recent evidence suggests that this type of response may also be triggered by cardiac overload after birth. In congenital heart disease, loading conditions are frequently abnormal, thereby affecting ventricular function. We ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2008.00853.x
更新日期：2008-03-01 00:00:00
abstract：:Clinical research on the deposition of inhaled substances (e.g. inhaled medications, airborne contaminants, fumes) in the lungs necessitates anatomical models of the airways. Current conducting airway models lack three-dimensional (3D) reality as little information is available in the literature on the distribution of...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.0021-8782.2001.00018.x
更新日期：2002-02-01 00:00:00
abstract：:Non-ionic, low-osmolar contrast agents (CAs) used for computed tomography, such as Optiray (ioversol) and Iomeron (iomeprol), are associated with the reduced risk of adverse reactions and toxicity in comparison with ionic CAs, such as Hexabrix. Hexabrix has previously been used for imaging articular cartilage but has ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.13271
更新日期：2020-12-01 00:00:00
abstract：:The bioavailability of polystyrene particles (1 microns) labelled with FITC (3M Company, Düsseldorf) was tested in rats after enteral administration. Since macromolecules and particles are preferentially transported in the lymph, the number of particles was counted in the lymph of the thoracic duct over a 6 h period. ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Schwann cells are excluded from the CNS during development by the glial limiting membrane, an area of astrocytic specialisation present at the nerve root transitional zone, and at blood vessels in the neuropil. This barrier, however, can be disrupted and, with the highly migratory nature of Schwann cells, can result i...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：10.1046/j.1469-7580.1997.19010035.x
更新日期：1997-01-01 00:00:00
abstract：:The collagen in the endoneurium is present as argyrophilic reticular fibres, which show up as thin, weakly birefringent greenish fibres when studied by aid of the Picrosirius-polarization method, and are composed of loosely arranged thin collagen fibrils. The epineurium consists of thick non-argyrophilic collagen fibr...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：:The proposal that the cytoplasmic granules of glomerular peripolar cells (PPCs) of the adult sheep and lamb contain a kallikrein-like substance has been re-examined. After extensive characterisation and purification of anti-kallikrein antibodies, it was concluded that PPCs do not contain immunohistochemically detectab...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1989-02-01 00:00:00
abstract：:A monoclonal antibody SBU-3 raised against an antigen expressed by the binucleate cells in the trophoblast of the sheep placentome crossreacts with an antigen expressed by binucleate cells in the placenta of the deer and the cow. This monoclonal antibody has been used to study the distribution of this subpopulation of...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:There is need for a consistent definition of structures caudal to the testis that variously are termed 'gubernaculum testis' as a basis for understanding the emergence and sexually dimorphic further growth and differentiation of this specifically mammalian structure. Rodent fetuses undergo a stage of development durin...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:It has been shown that gibbons are able to brachiate with very low mechanical costs. The conversion of muscle activity into smooth, purposeful movement of the limb depends on the morphometry of muscles and their mechanical action on the skeleton. Despite the gibbon's reputation for excellence in brachiation, little in...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2009.01109.x
更新日期：2009-09-01 00:00:00
abstract：:Interosseous sutures exhibit highly variable patterns of interdigitation and corrugation. Recent research has identified fundamental molecular mechanisms of suture formation, and computer models have been used to simulate suture morphogenesis. However, the role of bone strain in the development of complex sutures is l...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2011.01386.x
更新日期：2011-08-01 00:00:00
abstract：:Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer and are produce by many epithelial tissues in vertebrates. Osteopontin (OPN) is an adhesive phosphorylated glycoprotein that is expressed by a broad range of tissues and cells. Although gastric mucins MUC1, MUC5AC, M...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2010.01243.x
更新日期：2010-07-01 00:00:00
abstract：:The proximal stump of the optic nerve was examined by electron microscopy from 1 d to 8 wk (dpo/wpo) after intraorbital transection. At 1 dpo a layer of axonal, cytoplasmic and myelin debris approximately 15 microns thick was present at the cut end. A zone approximately 25 microns thick of abnormal and partly degenera...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:Although systematic reviews are conducted in the field of anatomical research, no instruments exist for the assessment of study quality. Thus, our objective was to develop a valid tool that reliably assesses the methodological quality of observational cadaveric studies. The QUACS scale (QUality Appraisal for Cadaveric...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12292
更新日期：2015-05-01 00:00:00
abstract：:The absolute density of the right and left os calcis has been measured in a group of healthy, sedentary volunteers. There was a significant difference between the density of the right and left os calcis in 90% of the subjects. Statistically, when all of the subjects were considered, the side on which the os calcis was...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1976-02-01 00:00:00
abstract：:The role of the neural arch in weight transmission in the cervical and upper thoracic regions of the vertebral column has been investigated. Measurements at the levels of C2, C4, C6, C7, T1 and T5 vertebrae were made in 44 adult male vertebral columns. At each level, the area of the inferior surface of the body was co...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Anomalous connection of the inferior caval vein to the left atrium is exceedingly rare, and has even been considered by some authors an anatomic and embryologic impossibility. This study demonstrates for the first time the existence of this rare malformation, diagnosed on prenatal echo, and confirmed on post-mortem ex...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.13378
更新日期：2020-12-20 00:00:00
abstract：:The three kinds of keratinized epithelium evident on gross examination of the soft palate of Wistar albino rats were studied by scanning and transmission electron microscopy. Scanning indicated a central area where the epithelium showed numerous fine folds and papillae, more lateral areas which were smooth, and most l...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1976-09-01 00:00:00
abstract：:An immuno-epifluorescence microscopic study of first trimester human chorionic villi has revealed different patterns of distribution for intermediate filament proteins. Keratin staining was restricted to the trophoblastic epithelium. The protein was shown to be concentrated into desmosome-containing apical and basal c...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
abstract：:The natural radiographic appearance of the various bones of the skeleton are described for several strains of laboratory mice. The Harwell substrains of CBA, A and 101 are generally similar and become osteoporotic on ageing. Harwell C57BL have similar, but more delicately chiseled, bones. Harwell C3H mice have bones w...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Lactating animals are particularly susceptible to mastitis during the early stages of mammary gland involution following weaning. In this study we compared the phagocytic capacity of cells collected from sheep mammary secretions at different stages of involution. The ability of neutrophils and macrophages to ingest la...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.0021-8782.2002.00104.x
更新日期：2002-11-01 00:00:00
abstract：:The retinal vasculature of postmortem normal human and diabetic eyes was studied using an immunohistochemical technique in conjunction with confocal laser scanning microscopy. The technique, which stained for von Willebrand factor, allowed both large areas of the retinal vasculature to be visualised and abnormalities ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1999.19410089.x
更新日期：1999-01-01 00:00:00
abstract：:To evaluate whether the lines occasionally detected on clinical magnetic resonance (MR) images are genuine hippocampal layers, a formalin fixed hippocampal specimen was scanned using T2 weighted sequences at 7 Tesla (voxel dimensions 0.064 x 0.064 x 1 mm) and at 1.5 Tesla (voxel dimensions: 0.156 x 0.156 x 1 mm) and c...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1999.19510131.x
更新日期：1999-07-01 00:00:00
