Abstract:
BACKGROUND:Vitamin D status [25(OH)D] has recently been reported to be associated with altered cellular bioenergetic profiles of peripheral blood mononuclear cells (PBMCs). No study has tracked the seasonal variation of 25(OH)D and its putative influence on whole body energy metabolism, cellular bioenergetic profiles, inflammatory markers and clinical chemistry. MATERIAL AND METHODS:Whole body energy metabolism and substrate utilisation were measured by indirect calorimetry. PBMCs obtained from the same subjects were isolated from whole blood, counted and freshly seeded. Bioenergetic analysis (mitochondrial stress test and glycolysis stress test) was performed using the Seahorse XFe96 flux analyser. 25(OH)D was assessed using the Architect immunoassay method. RESULTS:25(OH)D increased by a median (IQR) of 14.40 (20.13)nmol/L (p<0.001) from winter to summer and was accompanied by significant improvements in indices of insulin sensitivity, McAuley's index (p=0.019) and quantitative insulin sensitivity check index (p=0.028). PBMC mitochondrial parameters basal respiration, non-mitochondrial respiration, ATP production, proton leak, and maximal respiration decreased in summer compared to winter. Similarly, PBMC glycolytic parameters glycolytic activity, glucose response, and glycolytic capacity were all reduced in summer compared to winter. There was also a trend for absolute resting metabolic rate (RMR) to decrease (p=0.066). Markers of systemic inflammation MCP-1, IL-6, IL-8, IL-10, and IL-12p70 decreased significantly in summer compared to winter. Participants who entered winter with a low 25(OH)D (<50nmol/L), had the greatest alteration in bioenergetic parameters in summer, relative to those with winter 25(OH)D concentrations of 50-75nmol/L or >75nmol/L. The absolute change in 25(OH)D was not associated with altered bioenergetics. CONCLUSION:Seasonal improvements in 25(OH)D was associated with reduced systemic inflammation, PBMC bioenergetic profiles and whole body energy metabolism. These observational changes in PBMC bioenergetics were most pronounced in those who had insufficient 25(OH)D in winter. The data warrants confirmation through cause and effect study designs.
journal_name
Redox Bioljournal_title
Redox biologyauthors
Calton EK,Keane KN,Raizel R,Rowlands J,Soares MJ,Newsholme Pdoi
10.1016/j.redox.2017.04.009subject
Has Abstractpub_date
2017-08-01 00:00:00pages
814-820issn
2213-2317pii
S2213-2317(17)30059-9journal_volume
12pub_type
杂志文章相关文献
Redox Biology文献大全abstract:BACKGROUND:Platelets are essential for maintaining haemostasis and play a key role in the pathogenesis of cardiovascular disease. Upon ligation of platelet receptors through subendothelial matrix proteins, intracellular reactive oxygen species (ROS) are generated, further amplifying the platelet activation response. Th...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2015.10.009
更新日期:2015-12-01 00:00:00
abstract::Ozone (O3) is a serious public health concern. Recent findings indicate that the damaging health effects of O3 extend to multiple systemic organ systems. Herein, we hypothesize that O3 inhalation will cause downstream alterations to the liver. To test this, male Sprague-Dawley rats were exposed to 0.5ppm O3 for 8h/day...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2013.11.006
更新日期:2014-01-01 00:00:00
abstract::Oxidative modification of cysteine residues has been shown to regulate the activity of several protein-tyrosine kinases. We explored the possibility that Fms-like tyrosine kinase 3 (FLT3), a hematopoietic receptor-tyrosine kinase, is subject to this type of regulation. An underlying rationale was that the FLT3 gene is...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101325
更新日期:2020-01-01 00:00:00
abstract::Mistakenly thought to be the consequence of oxygen lack in contracting skeletal muscle we now know that the L-enantiomer of the lactate anion is formed under fully aerobic conditions and is utilized continuously in diverse cells, tissues, organs and at the whole-body level. By shuttling between producer (driver) and c...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2020.101454
更新日期:2020-08-01 00:00:00
abstract::Free radicals originate from both exogenous environmental sources and as by-products of the respiratory chain and cellular oxygen metabolism. Sustained accumulation of free radicals, beyond a physiological level, induces oxidative stress that is harmful for the cellular homeodynamics as it promotes the oxidative damag...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2014.01.017
更新日期:2014-01-30 00:00:00
abstract::We present a new database, specifically devoted to ROS homeostasis regulated proteins. This database replaced our previous database, the PeroxiBase, which was focused only on various peroxidase families. The addition of 20 new protein families related with ROS homeostasis justifies the new name for this more complex a...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101247
更新日期:2019-09-01 00:00:00
abstract::The nuclear protein HMGB1 (high mobility group box 1) is secreted by monocytes-macrophages in response to inflammatory stimuli and serves as a danger-associated molecular pattern. Acetylation and phosphorylation of HMGB1 are implicated in the regulation of its nucleocytoplasmic translocation for secretion, although in...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101203
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Macrophages regulate the inflammatory response and affect re-endothelialization. Inflammation and macrophages play important roles in promoting tissue repair, but p38α mitogen-activated protein kinase's role in re-endothelialization is unknown. METHODS AND RESULTS:Wire injuries of carotid arteries and Evans...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101775
更新日期:2021-01-01 00:00:00
abstract::Injury-induced stenosis is a serious vascular complication. We previously reported that p38α (MAPK14), a redox-regulated p38MAPK family member was a negative regulator of the VSMC contractile phenotype in vitro. Here we evaluated the function of VSMC-MAPK14 in vivo in injury-induced neointima hyperplasia and the under...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101137
更新日期:2019-04-01 00:00:00
abstract::Superoxide dismutase (SOD) is known to be protective against oxidative stress-mediated skin dysfunction. Here we explore the potential therapeutic activities of RM191A, a novel SOD mimetic, on skin. RM191A is a water-soluble dimeric copper (Cu2+-Cu3+)-centred polyglycine coordination complex. It displays 10-fold highe...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101790
更新日期:2021-01-01 00:00:00
abstract::Highly active anti-retrovirus therapy (HAART) has been used to block the progression and symptoms of human immunodeficiency virus infection. Although it decreases morbidity and mortality, clinical use of HAART has also been linked to various adverse effects such as severe cardiomyopathy resulting from compromised mito...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2017.06.011
更新日期:2017-10-01 00:00:00
abstract::The NADPH oxidase reaction produces protons. In the case of the NADPH oxidase, NOX2, activity depends on secretion of these protons and is inhibited by blockade of the voltage-gated proton channel (Hv1). Duox1 and Duox2 activities similarly produce intracellular protons but synthesize hydrogen peroxide directly instea...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101346
更新日期:2020-01-01 00:00:00
abstract::Oxidation-reduction chemistry is fundamental to the metabolism of all living organisms, and hence quantifying the principal redox players is important for a comprehensive understanding of cell metabolism in normal and pathological states. In mammalian cells, this is accomplished by measuring oxygen partial pressure (p...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101549
更新日期:2020-07-01 00:00:00
abstract::Red blood cells (RBC) have been considered almost exclusively as a transporter of metabolic gases and nutrients for the tissues. It is an accepted dogma that RBCs take up and inactivate endothelium-derived NO via rapid reaction with oxyhemoglobin to form methemoglobin and nitrate, thereby limiting NO available for vas...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2013.12.027
更新日期:2014-01-09 00:00:00
abstract::Oxidative stress plays a major part in the pathogenesis of drug-induced liver injury. Yet, overcoming it with other xenobiotics impose additional risks. In this study, we consider the use of natural-occurring and purified Vitamin E analogs as hepatoprotective agents. Vitamin E is well-known for its intrinsic antioxida...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2015.01.013
更新日期:2015-01-01 00:00:00
abstract::Redox signaling and oxidative stress are associated with tissue fibrosis and aging. Aging is recognized as a major risk factor for fibrotic diseases involving multiple organ systems, including that of the lung. A number of oxidant generating enzymes are upregulated while antioxidant defenses are deficient with aging a...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2016.06.005
更新日期:2016-10-01 00:00:00
abstract::Hypertonia is pathognomonic of cerebral palsy (CP), often caused by brain injury before birth. To understand the early driving events of hypertonia, we utilized magnetic resonance imaging (MRI) assessment of early critical brain injury in rabbit fetuses (79% term) that will predict hypertonia after birth following ant...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101407
更新日期:2020-01-01 00:00:00
abstract::Cells employ multiple defence mechanisms to sustain a wide range of stress conditions associated with accumulation of modified self-biomolecules leading to lipo- and proteotoxicity. One of such mechanisms involves activation of the autophagy-lysosomal pathway for removal and degradation of modified lipids, proteins an...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101123
更新日期:2019-05-01 00:00:00
abstract::Mitochondria are often regarded as a major source of reactive oxygen species (ROS) in animal cells, with H2O2 being the predominant ROS released from mitochondria; however, it has been recently demonstrated that energized brain mitochondria may act as stabilizers of H2O2 concentration (Starkov et al. [1]) based on the...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2015.05.001
更新日期:2015-08-01 00:00:00
abstract::Activity-induced nitric oxide (NO) imbalance and "nitrosative stress" are proposed mechanisms of disrupted Ca(2+) homeostasis in atrophic skeletal muscle. We thus mapped S-nitrosylated (SNO) functional muscle proteins in healthy male subjects in a long-term bed rest study (BBR2-2 Study) without and with exercise as co...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2013.10.006
更新日期:2013-10-28 00:00:00
abstract::Despite extensive research that has been carried out over the past three decades in the field of renal ischaemia-reperfusion (I/R) injury, the pathogenic role of mitochondrial fission in renal I/R injury is poorly understood. The aim of our study is to investigate the molecular mechanism by which mammalian STE20-like ...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.10.012
更新日期:2019-01-01 00:00:00
abstract::The generation of excessive amounts of reactive oxygen species (ROS) leads to cellular oxidative stress that underlies a variety of forms of hepatocyte injury and death including that from alcohol. Although ROS can induce cell damage through direct effects on cellular macromolecules, the injurious effects of ROS are m...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2014.09.004
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:The Ras family of small GTPases cycle between an inactive, GDP-bound state and an active, GTP-bound state. When bound to GTP, Ras engages and activates a number of effectors that mediate proliferative and survival signals. Ras is mutated in over 30% of human cancers, usually at codons 12, 13, or 61, to remai...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2015.09.015
更新日期:2015-08-01 00:00:00
abstract::Mitochondria are critical sources of hydrogen peroxide (H2O2), an important secondary messenger in mammalian cells. Recent work has shown that O2•-/H2O2 emission from individual sites of production in mitochondria is regulated by protein S-glutathionylation. Here, we conducted the first examination of O2•-/H2O2 releas...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2017.12.006
更新日期:2018-05-01 00:00:00
abstract::Gastric cancer is one of the leading causes of cancer-related deaths. Chemotherapy has improved long-term survival of patients with gastric cancer. Unfortunately, cancer readily develops resistance to apoptosis-inducing agents. New mechanisms, inducing caspase-independent paraptosis-like cell death in cancer cells is ...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.11.019
更新日期:2019-02-01 00:00:00
abstract::Autophagy is an important cell recycling program responsible for the clearance of damaged or long-lived proteins and organelles. Pharmacological modulators of this pathway have been extensively utilized in a wide range of basic research and pre-clinical studies. Bafilomycin A1 and chloroquine are commonly used compoun...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2016.11.004
更新日期:2017-04-01 00:00:00
abstract::Transcription factors control the rate of transcription of genetic information from DNA to messenger RNA, by binding specific DNA sequences in promoter regions. Transcriptional gene control is a rate-limiting process that is tightly regulated and based on transient environmental signals which are translated into long-...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2019.101104
更新日期:2019-02-01 00:00:00
abstract::Yeast Sir2 is an NAD-dependent histone deacetylase related to oxidative stress and aging. In a previous study, we showed that Sir2 is regulated by S-glutathionylation of key cysteine residues located at the catalytic domain. Mutation of these residues results in strains with increased resistance to disulfide stress. I...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101229
更新日期:2019-06-01 00:00:00
abstract::NADPH oxidase 5 (NOX5) is a transmembrane signaling enzyme that produces superoxide in response to elevated cytosolic calcium. In addition to its association with numerous human diseases, NOX5 has recently been discovered to play crucial roles in the immune response and cardiovascular system. Details of NOX5 maturatio...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101656
更新日期:2020-09-01 00:00:00
abstract::Culturing cells and tissues in vitro has provided valuable insights into the molecular mechanisms regulating redox signaling in cells with implications for medicine. However, standard culture techniques maintain mammalian cells in vitro under an artificial physicochemical environment such as ambient air and 5% CO2. Ox...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2014.04.008
更新日期:2014-04-18 00:00:00