Differentiating between apparent and actual rates of H2O2 metabolism by isolated rat muscle mitochondria to test a simple model of mitochondria as regulators of H2O2 concentration.

Abstract:

:Mitochondria are often regarded as a major source of reactive oxygen species (ROS) in animal cells, with H2O2 being the predominant ROS released from mitochondria; however, it has been recently demonstrated that energized brain mitochondria may act as stabilizers of H2O2 concentration (Starkov et al. [1]) based on the balance between production and the consumption of H2O2, the later of which is a function of [H2O2] and follows first order kinetics. Here we test the hypothesis that isolated skeletal muscle mitochondria, from the rat, are able to modulate [H2O2] based upon the interaction between the production of ROS, as superoxide/H2O2, and the H2O2 decomposition capacity. The compartmentalization of detection systems for H2O2 and the intramitochondrial metabolism of H2O2 leads to spacial separation between these two components of the assay system. This results in an underestimation of rates when relying solely on extramitochondrial H2O2 detection. We find that differentiating between these apparent rates found when using extramitochondrial H2O2 detection and the actual rates of metabolism is important to determining the rate constant for H2O2 consumption by mitochondria in kinetic experiments. Using the high rate of ROS production by mitochondria respiring on succinate, we demonstrate that net H2O2 metabolism by mitochondria can approach a stable steady-state of extramitochondrial [H2O2]. Importantly, the rate constant determined by extrapolation of kinetic experiments is similar to the rate constant determined as the [H2O2] approaches a steady state.

journal_name

Redox Biol

journal_title

Redox biology

authors

Treberg JR,Munro D,Banh S,Zacharias P,Sotiri E

doi

10.1016/j.redox.2015.05.001

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

216-224

issn

2213-2317

pii

S2213-2317(15)00044-0

journal_volume

5

pub_type

杂志文章
  • p38α in macrophages aggravates arterial endothelium injury by releasing IL-6 through phosphorylating megakaryocytic leukemia 1.

    abstract:BACKGROUND:Macrophages regulate the inflammatory response and affect re-endothelialization. Inflammation and macrophages play important roles in promoting tissue repair, but p38α mitogen-activated protein kinase's role in re-endothelialization is unknown. METHODS AND RESULTS:Wire injuries of carotid arteries and Evans...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2020.101775

    authors: Zhang M,Gao J,Zhao X,Zhao M,Ma D,Zhang X,Tian D,Pan B,Yan X,Wu J,Meng X,Yin H,Zheng L

    更新日期:2021-01-01 00:00:00

  • Clinical relevance of guanine-derived urinary biomarkers of oxidative stress, determined by LC-MS/MS.

    abstract::A reliable and fast liquid chromatography-tandem mass spectrometry method has been developed for the simultaneous determination of three oxidized nucleic acid damage products in urine, 8-oxoguanine (8-oxoGua), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo). We applied this me...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2018.11.016

    authors: Shih YM,Cooke MS,Pan CH,Chao MR,Hu CW

    更新日期:2019-01-01 00:00:00

  • Sexual hormones regulate the redox status and mitochondrial function in the brain. Pathological implications.

    abstract::Compared to other organs, the brain is especially exposed to oxidative stress. In general, brains from young females tend to present lower oxidative damage in comparison to their male counterparts. This has been attributed to higher antioxidant defenses and a better mitochondrial function in females, which has been li...

    journal_title:Redox biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.redox.2020.101505

    authors: Torrens-Mas M,Pons DG,Sastre-Serra J,Oliver J,Roca P

    更新日期:2020-04-01 00:00:00

  • Azidothymidine-triphosphate impairs mitochondrial dynamics by disrupting the quality control system.

    abstract::Highly active anti-retrovirus therapy (HAART) has been used to block the progression and symptoms of human immunodeficiency virus infection. Although it decreases morbidity and mortality, clinical use of HAART has also been linked to various adverse effects such as severe cardiomyopathy resulting from compromised mito...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2017.06.011

    authors: Nomura R,Sato T,Sato Y,Medin JA,Kushimoto S,Yanagisawa T

    更新日期:2017-10-01 00:00:00

  • Permanent cystathionine-β-Synthase gene knockdown promotes inflammation and oxidative stress in immortalized human adipose-derived mesenchymal stem cells, enhancing their adipogenic capacity.

    abstract::In the present study, we aimed to investigate the impact of permanent cystathionine-β-Synthase (CBS) gene knockdown in human telomerase reverse transcriptase (hTERT) immortalized human adipose-derived mesenchymal stem cells (ASC52telo) and in their capacity to differentiate into adipocytes. CBS gene KD in ASC52telo ce...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2020.101668

    authors: Comas F,Latorre J,Ortega F,Oliveras-Cañellas N,Lluch A,Ricart W,Fernández-Real JM,Moreno-Navarrete JM

    更新日期:2020-08-02 00:00:00

  • Hydrogen peroxide as a signal for skeletal muscle adaptations to exercise: What do concentrations tell us about potential mechanisms?

    abstract::Hydrogen peroxide appears to be the key reactive oxygen species involved in redox signalling, but comparisons of the low concentrations of hydrogen peroxide that are calculated to exist within cells with those previously shown to activate common signalling events in vitro indicate that direct oxidation of key thiol gr...

    journal_title:Redox biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.redox.2020.101484

    authors: Jackson MJ,Stretton C,McArdle A

    更新日期:2020-08-01 00:00:00

  • Winter to summer change in vitamin D status reduces systemic inflammation and bioenergetic activity of human peripheral blood mononuclear cells.

    abstract:BACKGROUND:Vitamin D status [25(OH)D] has recently been reported to be associated with altered cellular bioenergetic profiles of peripheral blood mononuclear cells (PBMCs). No study has tracked the seasonal variation of 25(OH)D and its putative influence on whole body energy metabolism, cellular bioenergetic profiles, ...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2017.04.009

    authors: Calton EK,Keane KN,Raizel R,Rowlands J,Soares MJ,Newsholme P

    更新日期:2017-08-01 00:00:00

  • Nitric oxide-mediated sensitization of resistant tumor cells to apoptosis by chemo-immunotherapeutics.

    abstract::The generation of NO by the various NO synthases in normal and malignant tissues is manifested by various biological effects that are involved in the regulation of cell survival, differentiation and cell death. The role of NO in the cytotoxic immune response was first revealed by demonstrating the induction of iNOS in...

    journal_title:Redox biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.redox.2015.08.013

    authors: Bonavida B,Garban H

    更新日期:2015-12-01 00:00:00

  • Curcuminoid B63 induces ROS-mediated paraptosis-like cell death by targeting TrxR1 in gastric cells.

    abstract::Gastric cancer is one of the leading causes of cancer-related deaths. Chemotherapy has improved long-term survival of patients with gastric cancer. Unfortunately, cancer readily develops resistance to apoptosis-inducing agents. New mechanisms, inducing caspase-independent paraptosis-like cell death in cancer cells is ...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2018.11.019

    authors: Chen X,Chen X,Zhang X,Wang L,Cao P,Rajamanickam V,Wu C,Zhou H,Cai Y,Liang G,Wang Y

    更新日期:2019-02-01 00:00:00

  • A novel role for NUPR1 in the keratinocyte stress response to UV oxidized phospholipids.

    abstract::Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators would be generated in primary human keratinocytes (KC) upon exposure to ultraviolet A light (UVA) and investigated the contribution of OxPL to UVA responses. Ma...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2018.11.006

    authors: Narzt MS,Nagelreiter IM,Oskolkova O,Bochkov VN,Latreille J,Fedorova M,Ni Z,Sialana FJ,Lubec G,Filzwieser M,Laggner M,Bilban M,Mildner M,Tschachler E,Grillari J,Gruber F

    更新日期:2019-01-01 00:00:00

  • Genome-wide transcriptional effects of deletions of sulphur metabolism genes in Drosophila melanogaster.

    abstract::In recent years, the gasotransmitter hydrogen sulphide (H2S), produced by the transsulphuration pathway, has been recognized as a biological mediator playing an important role under normal conditions and in various pathologies in both eukaryotes and prokaryotes. The transsulphuration pathway (TSP) includes the convers...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2020.101654

    authors: Zatsepina O,Karpov D,Chuvakova L,Rezvykh A,Funikov S,Sorokina S,Zakluta A,Garbuz D,Shilova V,Evgen'ev M

    更新日期:2020-09-01 00:00:00

  • Endothelial nitric oxide synthase in red blood cells: key to a new erythrocrine function?

    abstract::Red blood cells (RBC) have been considered almost exclusively as a transporter of metabolic gases and nutrients for the tissues. It is an accepted dogma that RBCs take up and inactivate endothelium-derived NO via rapid reaction with oxyhemoglobin to form methemoglobin and nitrate, thereby limiting NO available for vas...

    journal_title:Redox biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.redox.2013.12.027

    authors: Cortese-Krott MM,Kelm M

    更新日期:2014-01-09 00:00:00

  • Brain adaptation to hypoxia and hyperoxia in mice.

    abstract:AIMS:Hyperoxic breathing might lead to redox imbalance and signaling changes that affect cerebral function. Paradoxically, hypoxic breathing is also believed to cause oxidative stress. Our aim is to dissect the cerebral tissue responses to altered O2 fractions in breathed air by assessing the redox imbalance and the re...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2016.10.018

    authors: Terraneo L,Paroni R,Bianciardi P,Giallongo T,Carelli S,Gorio A,Samaja M

    更新日期:2017-04-01 00:00:00

  • Classifying oxidative stress by F2-isoprostane levels across human diseases: A meta-analysis.

    abstract::The notion that oxidative stress plays a role in virtually every human disease and environmental exposure has become ingrained in everyday knowledge. However, mounting evidence regarding the lack of specificity of biomarkers traditionally used as indicators of oxidative stress in human disease and exposures now necess...

    journal_title:Redox biology

    pub_type: 杂志文章,meta分析

    doi:10.1016/j.redox.2017.03.024

    authors: van 't Erve TJ,Kadiiska MB,London SJ,Mason RP

    更新日期:2017-08-01 00:00:00

  • Dynamic regulation of NADPH oxidase 5 by intracellular heme levels and cellular chaperones.

    abstract::NADPH oxidase 5 (NOX5) is a transmembrane signaling enzyme that produces superoxide in response to elevated cytosolic calcium. In addition to its association with numerous human diseases, NOX5 has recently been discovered to play crucial roles in the immune response and cardiovascular system. Details of NOX5 maturatio...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2020.101656

    authors: Sweeny EA,Schlanger S,Stuehr DJ

    更新日期:2020-09-01 00:00:00

  • Diphthamide affects selenoprotein expression: Diphthamide deficiency reduces selenocysteine incorporation, decreases selenite sensitivity and pre-disposes to oxidative stress.

    abstract::The diphthamide modification of translation elongation factor 2 is highly conserved in eukaryotes and archaebacteria. Nevertheless, cells lacking diphthamide can carry out protein synthesis and are viable. We have analyzed the phenotypes of diphthamide deficient cells and found that diphthamide deficiency reduces sele...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2018.09.015

    authors: Mayer K,Mundigl O,Kettenberger H,Birzele F,Stahl S,Pastan I,Brinkmann U

    更新日期:2019-01-01 00:00:00

  • Reactive species generated by heme impair alveolar epithelial sodium channel function in acute respiratory distress syndrome.

    abstract::We previously reported that the highly reactive cell-free heme (CFH) is increased in the plasma of patients with chronic lung injury and causes pulmonary edema in animal model of acute respiratory distress syndrome (ARDS) post inhalation of halogen gas. However, the mechanisms by which CFH causes pulmonary edema are u...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2020.101592

    authors: Aggarwal S,Lazrak A,Ahmad I,Yu Z,Bryant A,Mobley JA,Ford DA,Matalon S

    更新日期:2020-09-01 00:00:00

  • Keap1 controls protein S-nitrosation and apoptosis-senescence switch in endothelial cells.

    abstract::Premature senescence, a death escaping pathway for cells experiencing stress, is conducive to aging and cardiovascular diseases. The molecular switch between senescent and apoptotic fate remains, however, poorly recognized. Nrf2 is an important transcription factor orchestrating adaptive response to cellular stress. H...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2019.101304

    authors: Kopacz A,Klóska D,Proniewski B,Cysewski D,Personnic N,Piechota-Polańczyk A,Kaczara P,Zakrzewska A,Forman HJ,Dulak J,Józkowicz A,Grochot-Przęczek A

    更新日期:2020-01-01 00:00:00

  • Oxygen delivery, consumption, and conversion to reactive oxygen species in experimental models of diabetic retinopathy.

    abstract::Retinal tissue receives its supply of oxygen from two sources - the retinal and choroidal circulations. Decreases in retinal blood flow occur in the early stages of diabetes, with the eventual development of hypoxia thought to contribute to pathological neovascularization. Oxygen consumption in the retina has been fou...

    journal_title:Redox biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.redox.2014.04.006

    authors: Eshaq RS,Wright WS,Harris NR

    更新日期:2014-04-18 00:00:00

  • Nrf2 inhibition reverses the resistance of cisplatin-resistant head and neck cancer cells to artesunate-induced ferroptosis.

    abstract::Artesunate, an anti-malarial drug, has been repurposed as an anticancer drug due to its induction of cell death via reactive oxygen species (ROS) production. However, the molecular mechanisms regulating cancer cell death and the resistance of cells to artesunate remain unclear. We investigated the molecular mechanisms...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2016.12.010

    authors: Roh JL,Kim EH,Jang H,Shin D

    更新日期:2017-04-01 00:00:00

  • Oxidative stress-induced premature senescence dysregulates VEGF and CFH expression in retinal pigment epithelial cells: Implications for Age-related Macular Degeneration.

    abstract::Oxidative stress has a critical role in the pathogenesis of Age-related Macular Degeneration (AMD), a multifactorial disease that includes age, gene variants of complement regulatory proteins and smoking as the main risk factors. Stress-induced premature cellular senescence (SIPS) is postulated to contribute to this c...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2015.11.011

    authors: Marazita MC,Dugour A,Marquioni-Ramella MD,Figueroa JM,Suburo AM

    更新日期:2016-04-01 00:00:00

  • Distinct and overlapping functions of glutathione peroxidases 1 and 2 in limiting NF-κB-driven inflammation through redox-active mechanisms.

    abstract::Glutathione peroxidase 2 (GPx2) is one of the five selenoprotein GPxs having a selenocysteine in the active center. GPx2 is strongly expressed in the gastrointestinal epithelium, as is another isoform, GPx1, though with a different localization pattern. Both GPxs are redox-active enzymes that are important for the red...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2019.101388

    authors: Koeberle SC,Gollowitzer A,Laoukili J,Kranenburg O,Werz O,Koeberle A,Kipp AP

    更新日期:2020-01-01 00:00:00

  • Maintenance of mitochondrial genomic integrity in the absence of manganese superoxide dismutase in mouse liver hepatocytes.

    abstract::Manganese superoxide dismutase, encoded by the Sod2 gene, is a ubiquitously expressed mitochondrial antioxidant enzyme that is essential for mammalian life. Mice born with constitutive genetic knockout of Sod2 do not survive the neonatal stage, which renders the longitudinal study of the biochemical and metabolic effe...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2013.01.001

    authors: Cyr AR,Brown KE,McCormick ML,Coleman MC,Case AJ,Watts GS,Futscher BW,Spitz DR,Domann FE

    更新日期:2013-02-05 00:00:00

  • Response to: The mitochondria-targeted antioxidant MitoQ attenuates exercise-induced mitochondrial DNA damage (Williamson et al., available online 6 August 2020, 101,673).

    abstract::Williamson and colleagues present important data on the effects of MitoQ - an antioxidant compound targeted to mitochondria - on mtDNA damage following exercise. Future studies are needed to elucidate, whether or not the observed prevention of MitoQ on DNA damage is beneficial with regard to functional outcomes in hea...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2020.101732

    authors: Burtscher J,Burtscher M,Millet GP

    更新日期:2021-01-01 00:00:00

  • Ribosomal protein L10 in mitochondria serves as a regulator for ROS level in pancreatic cancer cells.

    abstract::Tumorigenesis is commonly known as a complicated process, in which reactive oxygen species (ROS) plays a critical role to involve in signal transduction, metabolism, cell proliferation and differentiation. Previously, ribosomal protein L10 (RPL10) was suggested to possess extra-ribosomal functions in pancreatic cancer...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2018.08.016

    authors: Yang J,Chen Z,Liu N,Chen Y

    更新日期:2018-10-01 00:00:00

  • An emerging perspective on sex differences: Intersecting S-nitrosothiol and aldehyde signaling in the heart.

    abstract::Cardiovascular disease is the leading cause of the death for both men and women. Although baseline heart physiology and the response to disease are known to differ by sex, little is known about sex differences in baseline molecular signaling, especially with regard to redox biology. In this review, we describe current...

    journal_title:Redox biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.redox.2020.101441

    authors: Casin KM,Kohr MJ

    更新日期:2020-04-01 00:00:00

  • Curcumin blunts epithelial-mesenchymal transition of hepatocytes to alleviate hepatic fibrosis through regulating oxidative stress and autophagy.

    abstract::The massive production and activation of myofibroblasts (MFB) is key to the development of liver fibrosis. In many studies, it has been proven that hepatocytes are an important part of MFB, and can be transformed into MFB through epithelial-mesenchymal transition (EMT) during hepatic fibrogenesis. In our previous stud...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2020.101600

    authors: Kong D,Zhang Z,Chen L,Huang W,Zhang F,Wang L,Wang Y,Cao P,Zheng S

    更新日期:2020-09-01 00:00:00

  • Redox control of yeast Sir2 activity is involved in acetic acid resistance and longevity.

    abstract::Yeast Sir2 is an NAD-dependent histone deacetylase related to oxidative stress and aging. In a previous study, we showed that Sir2 is regulated by S-glutathionylation of key cysteine residues located at the catalytic domain. Mutation of these residues results in strains with increased resistance to disulfide stress. I...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2019.101229

    authors: Vall-Llaura N,Mir N,Garrido L,Vived C,Cabiscol E

    更新日期:2019-06-01 00:00:00

  • Redoxins as gatekeepers of the transcriptional oxidative stress response.

    abstract::Transcription factors control the rate of transcription of genetic information from DNA to messenger RNA, by binding specific DNA sequences in promoter regions. Transcriptional gene control is a rate-limiting process that is tightly regulated and based on transient environmental signals which are translated into long-...

    journal_title:Redox biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.redox.2019.101104

    authors: Hopkins BL,Neumann CA

    更新日期:2019-02-01 00:00:00

  • Critical role of vascular peroxidase 1 in regulating endothelial nitric oxide synthase.

    abstract::Vascular peroxidase 1 (VPO1) is a member of the peroxidase family which aggravates oxidative stress by producing hypochlorous acid (HOCl). Our previous study demonstrated that VPO1 plays a critical role in endothelial dysfunction through dimethylarginine dimethylaminohydrolase2 (DDAH2)/asymmetric Dimethylarginine (ADM...

    journal_title:Redox biology

    pub_type: 杂志文章

    doi:10.1016/j.redox.2017.02.022

    authors: Liu Z,Liu Y,Xu Q,Peng H,Tang Y,Yang T,Yu Z,Cheng G,Zhang G,Shi R

    更新日期:2017-08-01 00:00:00