Abstract：
:A set of biomedically relevant iron oxide nanoparticles with systematically modified polymer surfaces was investigated regarding their interaction with the first contact partners after systemic administration such as blood cells, blood proteins, and the endothelial blood vessels, to establish structure-activity relationships. All nanoparticles were intensively characterized regarding their physicochemical parameters. Cyto- and hemocompatibility tests showed that (1) the properties of the core material itself were not relevant in short-term incubation studies, and (2) toxicities increased with higher polymer mass, neutral = anionic < cationic surface charge and charge density, as well as agglomeration. Based on this, it was possible to classify the nanoparticles in three groups, to establish structure-activity relationships and to predict nanosafety. While the results between cyto- and hemotoxicity tests correlated well for the polymers, data were not fully transferable for the nanoparticles, especially in case of cationic low molar mass polymer coatings. To evaluate the prediction efficacy of the static in vitro models, the results were compared to those obtained in an ex ovo shell-less hen's egg test after microinjection under dynamic flow conditions. While the polymers demonstrated hemotoxicity profiles comparable to the in vitro tests, the size-dependent risks of nanoparticles could be more efficiently simulated in the more complex ex ovo environment, making the shell-less egg model an efficient alternative to animal studies according to the 3R concept.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Schlenk F,Werner S,Rabel M,Jacobs F,Bergemann C,Clement JH,Fischer Ddoi
10.1007/s00204-017-1968-zsubject
Has Abstractpub_date
2017-10-01 00:00:00pages
3271-3286issue
10eissn
0340-5761issn
1432-0738pii
10.1007/s00204-017-1968-zjournal_volume
91pub_type
杂志文章abstract：:Spin trapping in vivo was first achieved in the author's laboratory and is shown to be a feasible method for demonstrating that highly reactive free radical intermediates are generated in the tissues of intact animals as a result of the exposure to certain toxic compounds and to ionizing radiation. The method is based...
journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/BF00296966
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journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/s00204-015-1517-6
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abstract：:The in ovo carcinogenicity assessing (IOCA) assay was used to examine the morphological changes in fetal turkey livers caused by the DNA-reactive carcinogen diethylnitrosamine (DEN). Fertilized turkey eggs were allocated into 3 groups: nondosed control (NDC), vehicle (water) control (VC) and DEN-dosed. At day 0, the f...
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doi：10.1007/s00204-010-0603-z
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journal_title：Archives of toxicology
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doi：10.1007/s00204-014-1392-6
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doi：10.1007/BF00288341
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pub_type： 杂志文章
doi：10.1007/s00204-014-1323-6
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00366186
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pub_type： 杂志文章
doi：10.1007/BF00303124
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pub_type： 杂志文章
doi：10.1007/s00204-003-0460-0
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00569233
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journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/BF00296984
更新日期：1987-01-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-014-1443-z
更新日期：2016-02-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/s00204-018-2192-1
更新日期：2018-07-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-004-0613-9
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1007/BF00364653
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pub_type： 杂志文章,评审
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更新日期：2016-07-01 00:00:00
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journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050243
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doi：10.1007/s00204-014-1260-4
更新日期：2015-02-01 00:00:00
abstract：:The comet assay is a versatile, reliable, cost-efficient, and fast technique for detecting DNA damage and repair in any tissue. It is useable in almost any cell type and applicable to both eukaryotic and prokaryotic organisms. Instead of highlighting one of the numerous specific aspects of the comet assay, the present...
journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/s00204-016-1767-y
更新日期：2016-10-01 00:00:00
abstract：:The reversibility of quinolone-induced cartilage lesions has not been studied in detail. We treated five groups of five to seven juvenile Wistar rats (male and female; age: 5 weeks) with 2 x 600 mg ofloxacin/kg by gastric intubation on 1 day only (9:00 a.m. and 5:00 p.m.) and studied the knee joints histologically 3 d...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s002040050301
更新日期：1996-01-01 00:00:00
abstract：:The organophosphorus (OP) pesticide malathion is a neurotoxic compound whose acute toxicity is primarily caused by the inhibition of acetylcholinesterase (AChE), leading to cholinergic syndrome-related symptoms. Some lines of evidence indicate that long-term exposure to low levels of OP may produce neuropsychiatric an...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-015-1466-0
更新日期：2016-03-01 00:00:00
abstract：:Purified phenobarbital-induced rat liver cytochrome P-450 was incorporated in a reconstituted system containing NADPH-cytochrome P-450 reductase, dilauroyl phosphatidyl choline and sodium cholate. This system was added to organ cultures of limb buds from mouse embryos on day 11 of gestation. Cyclophosphamide (100 micr...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/BF00293687
更新日期：1988-01-01 00:00:00
abstract：:It is estimated that only a few marketed drugs are able to directly induce liver steatosis. However, many other drugs may exacerbate or precipitate fatty liver in the presence of other risk factors or in patients prone to non-alcoholic fatty liver disease. On the other hand, current in vitro tests for drug-induced ste...
journal_title：Archives of toxicology
pub_type： 杂志文章
doi：10.1007/s00204-014-1197-7
更新日期：2014-04-01 00:00:00
abstract：:Drug-induced liver injuries have been a major focus of current research in drug development, and are also one of the major reasons for the failure and withdrawal of drugs in development. Drug-induced liver injuries have been systematically recorded in many public databases, which have become valuable resources in this...
journal_title：Archives of toxicology
pub_type： 杂志文章,评审
doi：10.1007/s00204-017-2024-8
更新日期：2017-09-01 00:00:00
