Abstract:
:Unsaturated compounds are known to cause false-positive reactions in the local lymph node assay (LLNA) but not in the guinea pig maximization test. We have tested a panel of substances (succinic acid, undecylenic acid, 1-octyn-3-ol, fumaric acid, maleic acid, linoleic acid, oleic acid, alpha-linolenic acid, squalene, and arachidonic acid) in the loose-fit coculture-based sensitization assay (LCSA) to evaluate whether unspecific activation of dendritic cells is a confounder for sensitization testing in vitro. Eight out of 10 tested substances caused significant up-regulation of CD86 on dendritic cells cocultured with keratinocytes and would have been classified as sensitizers; only succinic acid was tested negative, and squalene had to be excluded from data analysis due to poor solubility in cell culture medium. Based on human data, only undecylenic acid can be considered a true sensitizer. The true sensitizing potential of 1-octyn-3-ol is uncertain. Fumaric acid and its isomer maleic acid are not known as sensitizers, but their esters are contact allergens. A group of 18- to 20-carbon chain unsaturated fatty acids (linoleic acid, oleic acid, alpha-linolenic acid, and arachidonic acid) elicited the strongest reaction in vitro. This is possibly due to the formation of pro-inflammatory lipid mediators in the cell culture causing nonspecific activation of dendritic cells. In conclusion, both the LLNA and the LCSA seem to provide false-positive results for unsaturated fatty acids. The inclusion of T cells in dendritic cell-based in vitro sensitization assays may help to eliminate false-positive results due to nonspecific dendritic cell activation. This would lead to more accurate prediction of sensitizers, which is paramount for consumer health protection and occupational safety.
journal_name
Arch Toxicoljournal_title
Archives of toxicologyauthors
Frohwein TA,Sonnenburg A,Zuberbier T,Stahlmann R,Schreiner Mdoi
10.1007/s00204-015-1527-4subject
Has Abstractpub_date
2016-04-01 00:00:00pages
927-36issue
4eissn
0340-5761issn
1432-0738pii
10.1007/s00204-015-1527-4journal_volume
90pub_type
杂志文章abstract::Experimental manganese lymphadenopathy was produced by guinea pigs by intratracheal inoculation of manganese dioxide (50 mg/300 g body weight) and histopathologic changes in the tracheobronchial lymph nodes were studied up to 180 days. There was slow extracellular transport of manganese dust into the nodes, which did ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00351975
更新日期:1976-10-28 00:00:00
abstract::Environmental carcinogens-induced lung cancer and potential mechanisms have attracted widespread attention. Currently, microRNAs (miRNAs) have been recognized as key players in development of cancer, among which guide strand of miRNA has been well documented rather than its passenger strand (miRNA*). Our previous stud...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-015-1551-4
更新日期:2016-05-01 00:00:00
abstract::O,O,S-Trimethyl phosphorothioate (OOS-TMP) is known to induce unique symptoms, which are characterized by hypophagia, progressive weight loss, and hypothermia. To determine whether there is the possibility of a causal relationship between these toxic symptoms and a direct action of OOS-TMP on the central nervous syste...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050212
更新日期:1995-01-01 00:00:00
abstract::Rats were exposed to nickel sulfate (NiSO4) either by intratracheal (IT) instillation or by acute aerosol inhalation, and pulmonary clearance of Ni and pulmonary inflammatory responses were studied. The half-time of Ni in the lung (initial lung burden = 50 micrograms Ni/rat) was about 32 h in both the IT instillation ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050112
更新日期:1994-01-01 00:00:00
abstract::This review encompasses the most important advances in liver functions and hepatotoxicity and analyzes which mechanisms can be studied in vitro. In a complex architecture of nested, zonated lobules, the liver consists of approximately 80 % hepatocytes and 20 % non-parenchymal cells, the latter being involved in a seco...
journal_title:Archives of toxicology
pub_type: 杂志文章,评审
doi:10.1007/s00204-013-1078-5
更新日期:2013-08-01 00:00:00
abstract::Tacrolimus (FK506) and cyclosporin A (CsA) are two potent immunosuppressants mainly metabolized by hepatic cytochrome P-450 3A (CYP3A) monooxygenase. The aim of this study was to compare the toxic effects of the two drugs on hepatocytes in primary culture as a function of their metabolism and to explore the variations...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050499
更新日期:1998-04-01 00:00:00
abstract::A young drug consumer died after sniffing and drinking chloroform. The method to determine chloroform in body fluids and tissues is described. The influence of the oral and pulmonary absorption in the view to cause of death is discussed. ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00294994
更新日期:1983-08-01 00:00:00
abstract::The organophosphorus (OP) pesticide malathion is a neurotoxic compound whose acute toxicity is primarily caused by the inhibition of acetylcholinesterase (AChE), leading to cholinergic syndrome-related symptoms. Some lines of evidence indicate that long-term exposure to low levels of OP may produce neuropsychiatric an...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-015-1466-0
更新日期:2016-03-01 00:00:00
abstract::A method is described for the determination of lorazepam plasma levels involving extraction from the sample and analysis of the intact lorazepam by electron capture gas-liquid chromatography. Using mass spectrometry it is demonstrated, that lorazepam shows a thermal rearrangement under gas chromatographic conditions. ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00293571
更新日期:1976-06-08 00:00:00
abstract::The tissue distribution, metabolism and excretion of 14C-2,2',4,4',5-pentachlorodiphenyl ether (PCDE) were studied in the rat. Radioactivity was distributed in all tissues examined, with the highest concentrations being found in the fat followed by the skin, liver, kidney and muscle. Most of the radioactivity found in...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00332484
更新日期:1988-01-01 00:00:00
abstract::Renal papillary necrosis (RPN) was induced in Fischer 344 (F344) rats (n = 4) using 2-bromoethanamine hydrobromide (BEA) dosed at 150 mg/kg, and in multimammate desert mice (Mastomys natalensis) at 150 and 250 mg/kg (n = 4 per group). Control rats and Mastomys were dosed with 0.9% saline (n = 4 per group). Urine was c...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF02733668
更新日期:1995-01-01 00:00:00
abstract::Effects of the oxime HI-6, unrelated to reactivation of acetylcholinesterase (AChE), on field potentials in the dentate gyrus of the rat hippocampus following AChE inhibition, were investigated both in vitro and in vivo. In hippocampal slices, AChE inhibition decreased the perforant path evoked population spike amplit...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s002040050146
更新日期:1994-01-01 00:00:00
abstract::The present study was carried out to elucidate the mechanisms behind an increase in the incidence of malignant or multiple mammary tumors as a result of oral administration of rolipram in a 104-week carcinogenicity study. The organs and tissues of Sprague-Dawley (SD) rats of both sexes, which had been subjected to a 1...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-005-0016-6
更新日期:2006-02-01 00:00:00
abstract::Standard treatment of poisoning by organophosphates (OP) includes the administration of an antimuscarinic agent, e.g. atropine, and of an acetylcholinesterase (AChE) reactivator (oxime). The presently available oximes, obidoxime and pralidoxime (2-PAM), are considered to be insufficient for highly toxic OPs, e.g. sari...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-002-0375-1
更新日期:2002-09-01 00:00:00
abstract::Particle-induced carcinogenicity is not well understood, but might involve inflammation. The proinflammatory cytokine tumor necrosis factor (TNF) is considered to be an important mediator in inflammation. We investigated its role in particle-induced inflammation and DNA damage in mice with and without TNF signaling. T...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-004-0613-9
更新日期:2005-03-01 00:00:00
abstract::In vitro assays are often used for the hazard characterisation of compounds, but their application for quantitative risk assessment purposes is limited. This is because in vitro assays cannot provide a complete in vivo dose-response curve from which a point of departure (PoD) for risk assessment can be derived, like t...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-013-1107-4
更新日期:2013-09-01 00:00:00
abstract::Acetaminophen (APAP) is a widely-used analgesic and a known hepatotoxic agent. Vascular endothelial growth factor (VEGF) is a growth factor with multiple functional roles. VEGF plays an important role in angiogenesis and hepatic regeneration. The aim of this study was to determine the expression of VEGF isoforms and i...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-007-0201-x
更新日期:2007-10-01 00:00:00
abstract::A recent intervention study demonstrated the occurrence of irilone as second most abundant isoflavone next to daidzein in human plasma after consumption of a red clover-based dietary supplement (RCDS) containing predominately formononetin ≫ biochanin A > irilone (12 % of these isoflavones). To elucidate the relevance ...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-013-1114-5
更新日期:2014-02-01 00:00:00
abstract::Epidemiological and experimental studies have demonstrated the atherogenic effects of environmental toxicant arsenic and fluoride. Inflammatory mechanism plays an important role in the pathogenesis of atherosclerosis. The aim of the present study is to determine the effect of chronic exposure to arsenic and fluoride a...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-012-0803-9
更新日期:2012-06-01 00:00:00
abstract::Metabolism of isobutene (2-methylpropene) in rats (Sprague Dawley) and mice (B6C3F1) follows kinetics according to Michaelis-Menten. The maximal metabolic elimination rates are 340 mumol/kg/h for rats and 560 mumol/kg/h for mice. The atmospheric concentration at which Vmax/2 is reached is 1200 ppm for rats and 1800 pp...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF02034934
更新日期:1991-01-01 00:00:00
abstract::Chlorpyrifos (CPF) is a widely used organophosphorus pesticide. Earlier work from our laboratory and others has demonstrated that the sensitivity to CPF exposure changes markedly during maturation. A number of studies suggest that in addition to inhibiting acetylcholinesterase (AChE), CPF oxon may also interact direct...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-001-0309-3
更新日期:2002-01-01 00:00:00
abstract::Pregnane X receptor (PXR) is a nuclear receptor that plays a key regulatory role in xenobiotic metabolism in a ligand-dependent manner. Recently, ethanol was reported to be either an inducer or inhibitor of Cytochrome P450 (CYP) 3A expression. According to our recent microarray data, chronic ethanol upregulates the ex...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-014-1268-9
更新日期:2015-04-01 00:00:00
abstract::Exposure to toxic metals is a well-known problem in industrialized countries. Metals interfere with a number of physiological processes, including central nervous system (CNS), haematopoietic, hepatic and renal functions. In the evaluation of the toxicity of a particular metal it is crucial to consider many parameters...
journal_title:Archives of toxicology
pub_type: 杂志文章,评审
doi:10.1007/s00204-010-0544-6
更新日期:2010-07-01 00:00:00
abstract::Single doses of procarbazine (MIH) were injected IP at 0, 50, 100, 200, and 400 mg/kg body weight to CD-1 male mice. Activities of hyaluronidase, lactate dehydrogenase isoenzyme-X, and the dehydrogenases of sorbitol, alpha-glycerophosphate, glucose-6-phosphate, malate, isocitrate, and glyceraldehyde-3-phosphate in the...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00341017
更新日期:1984-10-01 00:00:00
abstract::Chronic ethanol consumption is a strong risk factor for the development of liver disease. Multiple mechanisms are involved in ethanol-mediated liver injury; oxidative stress being pointed has an important factor. However, it should be noted that moderate consumption of red wine has been associated with hepatoprotectiv...
journal_title:Archives of toxicology
pub_type: 杂志文章,评审
doi:10.1007/s00204-015-1538-1
更新日期:2015-10-01 00:00:00
abstract::Amoscanate, a substance which damages photoreceptors, was administered orally to Wistar rats in doses of 10, 40, and 125 mg/kg body weight once daily for 3 or 10 days. At both times electroretinographic, ophthalmological, and histopathological examinations of the retina were carried out to compare the sensitivity of c...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01973682
更新日期:1993-01-01 00:00:00
abstract::The effect of 1-deamino-2-tyr(ethyl) oxytocin (dEtOT) upon peri- and postnatal development of the rat was studied in two experiments when administered between day 15 of pregnancy and the day of parturition. The investigation showed that dEtOT did not affect the pups at birth, that dEtOT caused a dose-related growth re...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00324786
更新日期:1985-09-01 00:00:00
abstract::Age-related macular degeneration (AMD) is a late-onset retinal disease and the leading cause of central vision loss in the elderly. Degeneration of retinal pigment epithelial cells (RPE) is a crucial contributing factor responsible for the onset and progression of AMD. The toxic fluorophore N-retinyl-N-retinylidene et...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/s00204-019-02637-w
更新日期:2020-02-01 00:00:00
abstract::The effect of acute and chronic cadmium administration on hepatic drug metabolism was investigated in the male rat. 3 days after the acute administration of cadmium by either the intraperitoneal (0.84 mg Cd/kg) or the oral (greater than 80 mg Cd/kg) route, there was a significant potentiation in duration of hexobarbit...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF00310332
更新日期:1978-08-09 00:00:00
abstract::The pharmacokinetics of styrene were investigated in male Sprague-Dawley rats and male B6C3F1 mice using the closed chamber technique. Animals were exposed to styrene vapors of initial concentrations ranging from 550 to 5000 ppm, or received intraperitoneal (i.p.) doses of styrene from 20 to 340 mg/kg or oral (p.o.) d...
journal_title:Archives of toxicology
pub_type: 杂志文章
doi:10.1007/BF01969264
更新日期:1993-01-01 00:00:00