Development of a laboratory technique for the evaluation of protease enzymes activity in goat and sheep milk.

Abstract:

:The shelf life of milk and its products is strongly influenced by the quality of raw milk. Several bacteria present in raw milk can produce proteases: among them, those from psychotropic microorganisms (e.g. Bacillus subtilis), which produce enzymes under refrigeration, cause the most serious problem. The interaction of these enzymes with the milk proteins and the main proteolytic enzymes becomes important in determining the quality of the milk. An artificial substrate (azocasein) was used to quantify the enzyme activity through the release of a chromogenic product that was measured spectrophotometrically after clarifying the sample by the addition of trichloroacetic acid. The detection limit and quantification limit were 1.43 and 4.77mg/ml respectively for sheep milk and, 2.25 and 7.5mg/ml respectively for goat milk. This method is useful for determining the proteolytic activity in different media and its effectiveness depends on chemical-nutritional characteristics of the sample.

journal_name

Food Chem

journal_title

Food chemistry

authors

Palomba R,Formisano G,Arrichiello A,Auriemma G,Sarubbi F

doi

10.1016/j.foodchem.2016.10.125

subject

Has Abstract

pub_date

2017-04-15 00:00:00

pages

1637-1641

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(16)31804-0

journal_volume

221

pub_type

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