Abstract:
:The shelf life of milk and its products is strongly influenced by the quality of raw milk. Several bacteria present in raw milk can produce proteases: among them, those from psychotropic microorganisms (e.g. Bacillus subtilis), which produce enzymes under refrigeration, cause the most serious problem. The interaction of these enzymes with the milk proteins and the main proteolytic enzymes becomes important in determining the quality of the milk. An artificial substrate (azocasein) was used to quantify the enzyme activity through the release of a chromogenic product that was measured spectrophotometrically after clarifying the sample by the addition of trichloroacetic acid. The detection limit and quantification limit were 1.43 and 4.77mg/ml respectively for sheep milk and, 2.25 and 7.5mg/ml respectively for goat milk. This method is useful for determining the proteolytic activity in different media and its effectiveness depends on chemical-nutritional characteristics of the sample.
journal_name
Food Chemjournal_title
Food chemistryauthors
Palomba R,Formisano G,Arrichiello A,Auriemma G,Sarubbi Fdoi
10.1016/j.foodchem.2016.10.125subject
Has Abstractpub_date
2017-04-15 00:00:00pages
1637-1641eissn
0308-8146issn
1873-7072pii
S0308-8146(16)31804-0journal_volume
221pub_type
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