Abstract：
:A sensitive and reliable method was developed and validated for the simultaneous determination of 49 amino acids, B vitamins, flavonoids, and phenolic acids based on a rapid metabolomic extraction procedure combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) in a single chromatographic run and applied in analysis of 26 commonly consumed vegetables. The chromatographic and sample preparation conditions were optimized, given the high diversity of the target analytes. Eight isotope-labeled standards were applied to validate the method in terms of recovery, linearity, matrix effects, precision, and sensitivity. Most recoveries in four vegetable matrices ranged from 65.0% to 105.3% with associated RSDs < 20%. Low LOQs ranged from 0.06 to 17 µg/kg. Linear calibration curves with different ranges were established with R2 > 0.993. Among the 26 vegetables, purple cabbage, broccoli, and red lettuce were found to contain the highest concentrations of free amino acids, B vitamins, and phenolic compounds.
journal_name
Food Chemjournal_title
Food chemistryauthors
Zhao L,Zhao X,Xu Y,Liu X,Zhang J,He Zdoi
10.1016/j.foodchem.2020.128712subject
Has Abstractpub_date
2021-05-15 00:00:00pages
128712eissn
0308-8146issn
1873-7072pii
S0308-8146(20)32574-7journal_volume
344pub_type
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