Abstract：
:Delivery and expression of recombinant genes, a key methodology for many applications in biological research, remains a challenge especially for mature neurons. Here, we report easy, highly efficient and well tolerated transduction of adult peripheral and central neuronal populations of diverse species in culture using VSV-G pseudo-typed, recombinant baculovirus (BacMam). Transduction rates of up to 80% were reliably achieved at high multiplicity of infection without apparent neuro-cytopathic effects. Neurons could be transduced either shortly after plating or after several days in culture. Co-incubation with two different baculoviruses attained near complete co-localization of fluorescent protein expression, indicating multigene delivery. Finally, evidence for functional protein expression is provided by means of cre-mediated genetic recombination and neurite outgrowth assays. Recombinant protein was already detected within hours after transduction, thereby enabling functional readouts even in relatively short-lived neuronal cultures. Altogether, these results substantiate the usefulness of baculovirus-mediated transduction of mature neurons for future research in neuroscience.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Levin E,Diekmann H,Fischer Ddoi
10.1038/srep38928subject
Has Abstractpub_date
2016-12-13 00:00:00pages
38928issn
2045-2322pii
srep38928journal_volume
6pub_type
杂志文章abstract：:Laser Capture Microdissection (LCM) is a powerful tool to isolate and study gene expression pattern of desired and less accessible cells or tissues from a heterogeneous population. Existing LCM-based methods fail to obtain high quality RNA including small RNAs from small microdissected plant tissue and therefore, are ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21577
更新日期：2016-02-10 00:00:00
abstract：:Color vision requires the activity of cone photoreceptors to be compared in post-receptoral circuitry. Decades of psychophysical measurements have quantified the nature of these comparative interactions on a coarse scale. How such findings generalize to a cellular scale remains unclear. To answer that question, we qua...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26754-1
更新日期：2018-06-04 00:00:00
abstract：:In this study, 44 compounds in the petroleum ether extract of Maqian (Zanthoxylum myriacanthum var. pubescens) bark, a traditional Dai herbal medicine, were identified by GC-MS. Major components included 3(2H)-benzofuranone, asarinin and (dimethoxymethyl)-3-methoxy-benzene. A total of 18 compounds were isolated from t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45805
更新日期：2017-04-06 00:00:00
abstract：:IHC4 and PAM50 assays have been shown to provide additional prognostic information for patients with early breast cancer. We evaluated whether incorporating TP53 mutation analysis can further enhance their prognostic accuracy. We examined TP53 mutation and the IHC4 score in tumors of 605 patients diagnosed with stage ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17879
更新日期：2015-12-16 00:00:00
abstract：:STRs, scattered throughout the genome with higher mutation rate, are attractive to genetic application like forensic, anthropological and population genetics studies. STR profiling has now been applied in various aspects of human identification in forensic investigations. This work described the developmental validati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31075
更新日期：2016-08-08 00:00:00
abstract：:Vocal expression of emotions has been suggested to be conserved throughout evolution. However, since vocal indicators of emotions have never been compared between closely related species using similar methods, it remains unclear whether this is the case. Here, we investigated vocal indicators of emotional valence (neg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09437-1
更新日期：2017-08-18 00:00:00
abstract：:Acquisition of nutrients during intra-vacuolar growth of L. pneumophila within macrophages or amoebae is poorly understood. Since many genes of L. pneumophila are acquired by inter-kingdom horizontal gene transfer from eukaryotic hosts, we examined the presence of human solute carrier (SLC)-like transporters in the L....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26782-x
更新日期：2018-05-29 00:00:00
abstract：:The synthesis and subsequent genomic integration of DNA that is complementary to the genomes of non-retroviral RNA viruses are rarely observed. However, upon infection of various human cell lines and primary fibroblasts with the vesicular stomatitis virus (VSV), we detected DNA complementary to the VSV RNA. The VSV DN...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05074
更新日期：2014-05-30 00:00:00
abstract：:Nonalcoholic fatty liver disease (NAFLD) is an important risk factor for the development of type 2 diabetes mellitus. Interferon gamma-induced protein 10 (IP-10), a proinflammatory chemokine, plays a crucial role in inflammatory diseases. This cross-sectional pilot study investigated whether circulating IP-10 is assoc...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/srep10096
更新日期：2015-05-11 00:00:00
abstract：:We report on an all-dielectric, polarization-independent angular filter with one-dimension (1D) photonic crystal (PC) composed of semiconductor compatible Si/SiO2 pairs. The near-symmetric directional band gap of p- and s-polarized components and Fabry-Pérot (F-P) resonances are utilized to realize efficient polarizat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16837-w
更新日期：2017-11-29 00:00:00
abstract：:Liver repopulation by transplanted hepatocytes has not been achieved previously in a normal liver microenvironment. Here we report that adult rat hepatocytes transduced ex vivo with a lentivirus expressing a human YapERT2 fusion protein (hYapERT2) under control of the hepatocyte-specific transthyretin (TTR) promoter r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19275
更新日期：2016-01-14 00:00:00
abstract：:Prostate cancer (PCa) is the most common solid neoplasm diagnosed in developed countries. Nerve-sparing radical prostatectomy (NS-RP) has been widely accepted as the best choice treatment for localised PCa. However, erectile dysfunction (ED) and urinary incontinence are commonly observed after NS-RP. Using meta-analys...
journal_title：Scientific reports
pub_type： 杂志文章,评审
doi：10.1038/srep05801
更新日期：2014-07-23 00:00:00
abstract：:Studies of personality have suggested that dissimilarities in ability to cope with stressful situations results in differing tendency to develop addictive behaviors. The present study used selectively bred stress-resilient, socially-dominant (Dom) and stress-vulnerable, socially-submissive (Sub) mice to investigate th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19816-x
更新日期：2018-01-29 00:00:00
abstract：:The Toarcian Oceanic Anoxic Event (TOAE, Early Jurassic, ~182 Ma ago) was characterised by severe environmental perturbations which led to habitat degradation and extinction of marine species. Warming-induced anoxia is usually identified as main driver, but because marine life was also affected in oxygenated environme...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61393-5
更新日期：2020-03-13 00:00:00
abstract：:Nitric oxide (NO) signaling has been studied in the eye, including in the pathophysiology of some eye diseases. While NO production by nitric oxide synthase (NOS) enzymes in the eye has been characterized, the more recently described pathways of NO generation by nitrate (NO3-) and nitrite (NO2-) ions reduction has rec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69272-9
更新日期：2020-08-05 00:00:00
abstract：:The flagellum is a rotary motor that enables bacteria to swim in liquids and swarm over surfaces. Numerous global regulators control flagellar assembly in response to cellular and environmental factors. Previous studies have also shown that flagellar assembly is affected by the growth-rate of the cell. However, a syst...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41189
更新日期：2017-01-24 00:00:00
abstract：:An accurate knowledge of tissue optical properties (absorption coefficients, μa, and reduced scattering coefficients, μs') is critical for precise modeling of light propagation in biological tissue, essential for developing diagnostic and therapeutic optical techniques that utilize diffusive photons. A great number of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45736-5
更新日期：2019-06-24 00:00:00
abstract：:Sex hormones fluctuate during the menstrual cycle. Evidence from animal studies suggests similar subtle fluctuations in hippocampal structure, predominantly linked to estrogen. Hippocampal abnormalities have been observed in several neuropsychiatric pathologies with prominent sexual dimorphism. Yet, the potential impa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32833
更新日期：2016-10-07 00:00:00
abstract：:Bovine milk is vital for infant nutrition and is a major component of the human diet. Bovine mastitis is a common inflammatory disease of mammary gland in cattle. It alters the immune profile of the animal and lowers the quality and yield of milk causing huge economic losses to dairy industry. The incidence of sub-cli...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66211-6
更新日期：2020-06-09 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-61546-6
更新日期：2020-03-06 00:00:00
abstract：:The aim of this study was to estimate the incidence of T2DM in three population groups: rural, rural-to-urban migrants and urban dwellers. Data from the PERU MIGRANT Study was analysed. The baseline assessment was conducted in 2007-2008 using a single-stage random sample and further follow-up was undertaken in 2015-16...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23812-6
更新日期：2018-04-03 00:00:00
abstract：:Gene expression profiles reflect the biologically diverse activities of cells under specific cell environments. Using the transcriptional response of cultured cells to blood composition, we developed a litmus gene assay to discriminate blood samples reflecting different sample qualities or disease conditions. This cel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17293-2
更新日期：2017-12-04 00:00:00
abstract：:Olfactory dysfunction is a robust and early sign for Parkinson's disease (PD). Previous studies have revealed its association with dementia and related neural changes in PD. Yet, how olfactory dysfunction affects white matter (WM) microstructure in newly diagnosed and untreated PD remains unclear. Here we comprehensiv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12947-7
更新日期：2017-10-02 00:00:00
abstract：:New clinical trials for diabetic macular oedema (DMO) are being designed to prove superiority over aflibercept when this agent is already very effective in improving visual acuity (VA) and DMO. The aim of this study was to determine the optimal inclusion-exclusion criteria for trials to aim for superiority in visual o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73571-6
更新日期：2020-10-05 00:00:00
abstract：:Epstein Barr Virus (EBV) is a human herpesvirus, and has been reported to be associated with nasopharyngeal carcinoma, gastric carcinoma, Burkitt's lymphoma and Hodgkin's lymphoma. In most of the associated tumors, the virus remains in a latently infected state. During latency, EBV expresses Latent Membrane Protein 2A...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62081-0
更新日期：2020-03-25 00:00:00
abstract：:During pregnancy a variety of immunological changes occur to accommodate the fetus. It is unknown whether these changes continue to affect humoral immunity postpartum or how quickly they resolve. IgG levels were measured to P. falciparum and P. vivax antigens in 201 postpartum and 201 controls over 12 weeks. Linear mi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32159
更新日期：2016-08-25 00:00:00
abstract：:Giardia is a worldwide spread protozoan parasite colonizing in small intestines of vertebrates, causing Giardiasis. The controversy about whether it is an extremely primitive eukaryote or just a highly evolved parasite has become a fetter to its uses as a model for both evolutionary and parasitological studies for yea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10054-1
更新日期：2017-08-25 00:00:00
abstract：:Anatomical features of first maxillary premolars may greatly affect endodontic and following restorative treatments. The aim of this study was to evaluate root canal configuration and root wall thickness of first maxillary premolars using a preexisting CBCT database. A CBCT database of 400 first maxillary premolar was...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56957-z
更新日期：2020-01-16 00:00:00
abstract：:CD134 (OX40) is a member of the tumour necrosis factor receptor superfamily (TNFRSF). It acts as a costimulatory receptor on T cells, but its role on NK cells is poorly understood. CD137, another TNFRSF member has been shown to enhance the anti-tumour activity of NK cells in various malignancies. Here, we examine the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20656-y
更新日期：2018-02-02 00:00:00
abstract：:The corrosion of steel in concrete is mainly due to the chemical reaction between the chloride ions and iron ions. Indeed, this is a serious threaten for reinforced concrete structure, especially for the reinforced concrete structure in the sea. So it is urgent and important to protect concrete against chloride ions c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03359
更新日期：2013-11-28 00:00:00
