Abstract：
:αβ T cells respond to peptide epitopes presented by major histocompatibility complex (MHC) molecules. The role of T cell receptor (TCR) germline complementarity determining regions (CDR1 and 2) in MHC restriction is not well understood. Here, we examine T cell development, MHC restriction and antigen recognition where germline CDR loop structure has been modified by multiple glycine/alanine substitutions. Surprisingly, loss of germline structure increases TCR engagement with MHC ligands leading to excessive loss of immature thymocytes. MHC restriction is, however, strictly maintained. The peripheral T cell repertoire is affected similarly, exhibiting elevated cross-reactivity to foreign peptides. Our findings are consistent with germline TCR structure optimising T cell cross-reactivity and immunity by moderating engagement with MHC ligands. This strategy may operate alongside co-receptor imposed MHC restriction, freeing germline TCR structure to adopt this novel role in the TCR-MHC interface.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Attaf M,Holland SJ,Bartok I,Dyson Jdoi
10.1038/srep35006subject
Has Abstractpub_date
2016-10-24 00:00:00pages
35006issn
2045-2322pii
srep35006journal_volume
6pub_type
杂志文章abstract：:Milk microbiota has a great influence on the safety and quality of dairy products. However, few studies have investigated the variations of bacterial composition in raw milk. In this study, raw milk samples were collected in 12 successive months, and their bacterial compositions were determined by 16 S rRNA gene seque...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20862-8
更新日期：2018-02-05 00:00:00
abstract：:TP53 mutation (i.e., loss of normal-p53) may evoke epithelial-mesenchymal transition (EMT), which was previously attributed to loss of certain miRNAs. However, not all epithelial cells undergo EMT upon TP53 mutation, and the p53-miRNA axis may not fully explain p53 function in epithelial integrity. We here show two mo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20043-7
更新日期：2018-01-25 00:00:00
abstract：:Seagrass meadows are threatened by coastal development and global change. In the face of these pressures, molecular techniques such as reverse transcription quantitative real-time PCR (RT-qPCR) have great potential to improve management of these ecosystems by allowing early detection of chronic stress. In RT-qPCR, the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17051
更新日期：2015-11-23 00:00:00
abstract：:Histone lysine methyltransferases (KMTs) play an important role in epigenetic gene regulation and have emerged as promising targets for drug discovery. However, the scope and limitation of KMT catalysis on substrates possessing substituted lysine side chains remain insufficiently explored. Here, we identify new unnatu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78331-0
更新日期：2020-12-09 00:00:00
abstract：:Mortality of glioblastoma multiforme (GBM) has not improved over the last two decades despite medical breakthroughs in the treatment of other types of cancers. Nanoparticles hold tremendous promise to overcome the pharmacokinetic challenges and off-target adverse effects. However, an inhibitory effect of nanoparticles...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59146-5
更新日期：2020-02-11 00:00:00
abstract：:Photobiomodulation-based (LLLT) therapies show tantalizing promise for treatment of skin diseases. Confidence in this approach is blighted however by lamentable inconsistency in published experimental designs, and so complicates interpretation. Here we interrogate the appropriateness of a range of previously-reported ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02802-0
更新日期：2017-06-05 00:00:00
abstract：:Microorganisms associated with plants are highly diverse and can produce a large number of secondary metabolites, with antimicrobial, anti-parasitic and cytotoxic activities. We are particularly interested in exploring endophytes from medicinal plants found in the Pantanal, a unique and widely unexplored wetland in Br...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21400-2
更新日期：2018-02-15 00:00:00
abstract：:Glomerular mesangial cell (GMC) proliferation and matrix expansion are pathological hallmarks of a wide range of kidney diseases, including diabetic nephropathy. Although the circulating level of peptide hormone urotensin II (UII) and kidney tissue expression of UII and UII receptors (UTR) are increased in diabetic ne...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18143-x
更新日期：2017-12-22 00:00:00
abstract：:Zika virus (ZIKV) is an arboviral infection that has been shown to be sexually transmitted. The study outlined herein aims to determine if accessory sex glands and epididymal epithelial cells are sources of viral persistence in subacute and chronic ZIKV infection, and if infection of these organs is important in sexua...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44582-9
更新日期：2019-06-06 00:00:00
abstract：:Myositis is characterised by muscle inflammation and weakness. Although generally thought to be driven by a systemic autoimmune response, increasing evidence suggests that intrinsic changes in the muscle might also contribute to the pathogenesis. Long non-coding RNAs (lncRNAs) are a family of novel genes that regulate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08603-9
更新日期：2017-08-14 00:00:00
abstract：:The circadian clock confers daily rhythmicity on many biochemical and physiological functions and its disruption is associated with increased risks of developing obesity, diabetes, heart disease and cancer. Although, there are studies on the role of Bmal1 in carcinogenesis using germline, conditional or tissue-specifi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34433-4
更新日期：2018-10-30 00:00:00
abstract：:Disease associated genetic variations often cause intracellular enzyme inactivation, dysregulation and instability. However, allosteric communication of mutational effects to distant functional sites leading to loss-of-function remains poorly understood. We characterize here interdomain site-to-site communication by w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44532
更新日期：2017-03-14 00:00:00
abstract：:Recent successes in malaria control have been largely attributable to the deployment of insecticide-based vector control tools such as bed nets and indoor residual spraying. Pyrethroid-treated bed nets are acutely neurotoxic to mosquitoes, inducing symptoms such as loss of coordination, paralysis, and violent spasms. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46674
更新日期：2017-04-25 00:00:00
abstract：:RAS genotyping is mandatory to predict anti-EGFR monoclonal antibodies (mAbs) therapy resistance and BRAF genotyping is a relevant prognosis marker in patients with metastatic colorectal cancer. Although the role of hotspot mutations is well defined, the impact of uncommon mutations is still unknown. In this study, we...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43646-0
更新日期：2019-05-08 00:00:00
abstract：:Non-Alcoholic Fatty Liver Disease (NAFLD) represents the most common form of chronic liver injury and can progress to cirrhosis and hepatocellular carcinoma. A "multi-hit" theory, involving high fat diet and signals from the gut-liver axis, has been hypothesized. The role of the NLRP3-inflammasome, which senses danger...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11744-6
更新日期：2017-09-22 00:00:00
abstract：:Mixed Plasmodium malaria infections can lead to severe malaria. This systematic review and meta-analysis aimed to explore the prevalence of severe mixed Plasmodium malaria infection and to compare it with the prevalence of severe P. falciparum malaria mono-infection across the included studies. Original English-langua...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-020-68082-3
更新日期：2020-07-06 00:00:00
abstract：:Guidelines endorse targeted temperature management to reduce neurological sequelae and mortality after cardiac arrest (CA). Additional therapeutic approaches are lacking. Inhaled nitric oxide (iNO) given post systemic ischemia/reperfusion injury improves outcomes. Attenuated inflammation by iNO might be crucial in bra...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25213-1
更新日期：2018-04-30 00:00:00
abstract：:Heme-copper oxygen reductases are terminal respiratory enzymes, catalyzing the reduction of dioxygen to water and the translocation of protons across the membrane. Oxygen consumption is inhibited by various substances. Here we tested the relatively unknown inhibition of cytochrome c oxidase (CcO) with isocyanate. In c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60801-0
更新日期：2020-03-02 00:00:00
abstract：:Previous studies showed that better adherence to the Mediterranean diet (MD) is associated with lower risk of chronic diseases, but limited data are available on bone health. We investigated the association of the MD with bone mineral density (BMD) in Chinese adults. We included 2371 participants aged 40-75 years in t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25662
更新日期：2016-05-09 00:00:00
abstract：:Promoter activation drives gene transcriptional output. Here we report generating site-specifically integrated single-copy promoter transgenes and measuring their expression to indicate promoter activities at single-mRNA level. mRNA counts, Pol II density and Pol II firing rates of the Ccnb1 promoter transgene resembl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02240-y
更新日期：2017-05-18 00:00:00
abstract：:Living organisms employ various mechanisms to escape harm. At the cellular level, mobile cells employ movement to avoid harmful chemicals or repellents. The present study is the first to report that cells move away from the site of injury in response to local wounding. When a migrating Dictyostelium cell was locally w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39678-1
更新日期：2019-03-12 00:00:00
abstract：:The adaptation of herbivorous insects to various host plants facilitates the spread and outbreak of many important invasive pests, however, the molecular mechanisms that underneath this process are poorly understood. In the past three decades, two species of the whitefly Bemisia tabaci complex, Middle East-Asia Minor ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12387-3
更新日期：2017-09-21 00:00:00
abstract：:We investigated whether clinical factors including comorbidities, medications, and laboratory results predict inpatient fall risk in older adults. The participants in this case-control study included hospitalized older adults with acute conditions who had falls during their hospital stay (case group) and 410 hospitali...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81034-9
更新日期：2021-01-15 00:00:00
abstract：:Mitochondrial DNA (mtDNA) mutations are commonly found in the skeletal muscle of patients with mitochondrial disease, inflammatory myopathies and sarcopenia. The majority of these mutations are mtDNA deletions, which accumulate to high levels in individual muscle fibres causing a respiratory defect. Most mtDNA deletio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09906
更新日期：2015-05-19 00:00:00
abstract：:Helicobacter pylori is presumed to infect gastric tissue via the oral cavity in childhood, whereas risk factors for H. pylori infection in the oral cavity are unknown. In this study, we analysed the effects of Streptococcus mutans, a major cariogenic bacterial species, on H. pylori colonisation in the oral cavity, as ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69368-2
更新日期：2020-07-27 00:00:00
abstract：:We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image si...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03615
更新日期：2014-01-09 00:00:00
abstract：:Compact and power-efficient plastic electronic synapses are of fundamental importance to overcoming the bottlenecks of developing a neuromorphic chip. Memristor is a strong contender among the various electronic synapses in existence today. However, the speeds of synaptic events are relatively slow in most attempts at...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01619
更新日期：2013-01-01 00:00:00
abstract：:Solitons, defined as nonlinear waves which can reflect from boundaries or transmit through each other, are found in conservative, fully integrable systems. Similar phenomena, dubbed quasi-solitons, have been observed also in dissipative, "excitable" systems, either at finely tuned parameters (near a bifurcation) or in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30879
更新日期：2016-08-05 00:00:00
abstract：:In this study, we made a comparative efficacy and safety assessment of two different fixed combinations of drugs, viz., tafluprost/timolol (TAF/TIM) and latanoprost/carteolol (LAT/CAR), by determining their effects on intraocular pressure (IOP) in ocular normotensive monkeys and examining their toxic effects on ocular...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44028-2
更新日期：2019-05-16 00:00:00
abstract：:Magnetic resonance imaging (MRI) has evolved into an outstandingly versatile diagnostic modality, as it has the ability to non-invasively produce detailed information on a tissue's structure and function. Complementary data is normally obtained in separate measurements, either as contrast-weighted images, which are fa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44832-w
更新日期：2019-06-11 00:00:00
