Uncommon mutational profiles of metastatic colorectal cancer detected during routine genotyping using next generation sequencing.

Abstract:

:RAS genotyping is mandatory to predict anti-EGFR monoclonal antibodies (mAbs) therapy resistance and BRAF genotyping is a relevant prognosis marker in patients with metastatic colorectal cancer. Although the role of hotspot mutations is well defined, the impact of uncommon mutations is still unknown. In this study, we aimed to discuss the potential utility of detecting uncommon RAS and BRAF mutation profiles with next-generation sequencing. A total of 779 FFPE samples from patients with metastatic colorectal cancer with valid NGS results were screened and 22 uncommon mutational profiles of KRAS, NRAS and BRAF genes were selected. In silico prediction of mutation impact was then assessed by 2 predictive scores and a structural protein modelling. Three samples carry a single KRAS non-hotspot mutation, one a single NRAS non-hotspot mutation, four a single BRAF non-hotspot mutation and fourteen carry several mutations. This in silico study shows that some non-hotspot RAS mutations seem to behave like hotspot mutations and warrant further examination to assess whether they should confer a resistance to anti-EGFR mAbs therapy for patients bearing these non-hotspot RAS mutations. For BRAF gene, non-V600E mutations may characterise a novel subtype of mCRC with better prognosis, potentially implying a modification of therapeutic strategy.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Franczak C,Kandathil SM,Gilson P,Husson M,Rouyer M,Demange J,Leroux A,Merlin JL,Harlé A

doi

10.1038/s41598-019-43646-0

subject

Has Abstract

pub_date

2019-05-08 00:00:00

pages

7083

issue

1

issn

2045-2322

pii

10.1038/s41598-019-43646-0

journal_volume

9

pub_type

杂志文章
  • Radiocaesium partitioning in Japanese cedar forests following the "early" phase of Fukushima fallout redistribution.

    abstract::Our study focused on radiocaesium (137Cs) partitioning in forests, three vegetation periods after the Fukushima Daiichi nuclear power plant accident. 137Cs distribution in forest components (organic and mineral soil layers as well as tree compartments: stem, bark, needles, branches and roots) was measured for two Japa...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep37618

    authors: Coppin F,Hurtevent P,Loffredo N,Simonucci C,Julien A,Gonze MA,Nanba K,Onda Y,Thiry Y

    更新日期:2016-11-23 00:00:00

  • Discovering neutralizing antibodies targeting the stem epitope of H1N1 influenza hemagglutinin with synthetic phage-displayed antibody libraries.

    abstract::Broadly neutralizing antibodies developed from the IGHV1-69 germline gene are known to bind to the stem region of hemagglutinin in diverse influenza viruses but the sequence determinants for the antigen recognition, including neutralization potency and binding affinity, are not clearly understood. Such understanding c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15053

    authors: Tung CP,Chen IC,Yu CM,Peng HP,Jian JW,Ma SH,Lee YC,Jan JT,Yang AS

    更新日期:2015-10-12 00:00:00

  • Biomimetic cellular vectors for enhancing drug delivery to the lungs.

    abstract::Despite recent advances in drug delivery, the targeted treatment of unhealthy cells or tissues continues to remain a priority. In cancer (much like other pathologies), delivery vectors are designed to exploit physical and biological features of unhealthy tissues that are not always homogenous across the disease. In so...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-55909-x

    authors: Evangelopoulos M,Yazdi IK,Acciardo S,Palomba R,Giordano F,Pasto A,Sushnitha M,Martinez JO,Basu N,Torres A,Hmaidan S,Parodi A,Tasciotti E

    更新日期:2020-01-13 00:00:00

  • A Bio-inspired Hypoxia Sensor using HIF1a-Oxygen-Dependent Degradation Domain.

    abstract::Functional imaging has become an important tool in oncology because it not only provides information about the size and localization of the tumour, but also about the pathophysiological features of the tumoural cells. One of the characteristic features of some tumour types is that their fast growth leads to deficient ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-43618-4

    authors: Iglesias P,Penas C,Barral-Cagiao L,Pazos E,Costoya JA

    更新日期:2019-05-08 00:00:00

  • Study of temporal variability of salivary cortisol and cortisone by LC-MS/MS using a new atmospheric pressure ionization source.

    abstract::There is a growing interest concerning the relevance of salivary cortisone levels in stress-related research. However, studies investigating morning patterns and day-to-day variability of cortisone versus cortisol levels are lacking. Cortisol and cortisone analysis by liquid chromatography-tandem mass spectroscopy (LC...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-55571-3

    authors: Bakusic J,De Nys S,Creta M,Godderis L,Duca RC

    更新日期:2019-12-17 00:00:00

  • Relationship between Lipoprotein (a) and cognitive function - Results from the Berlin Aging Study II.

    abstract::It has been suggested that an age-related loss of cognitive function might be driven by atherosclerotic effects associated with altered lipid patterns. However, the relationship between Lipoprotein (a) [Lp(a)] and healthy cognitive aging has not yet been sufficiently investigated. For the current analysis we used the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-66783-3

    authors: Röhr F,Bucholtz N,Toepfer S,Norman K,Spira D,Steinhagen-Thiessen E,Lill CM,Bertram L,Demuth I,Buchmann N,Düzel S

    更新日期:2020-06-30 00:00:00

  • Maternal depression attenuates newborn vitamin D concentrations in winter-spring: a prospective population-based study.

    abstract::We aimed to investigate whether the newborns of mothers with maternal depression (MD) had lower vitamin D levels than newborns of non-MD (NMD) mothers and identify the potential mechanism underlying this association. Maternal depressive symptoms in late pregnancy and concentrations of cord blood 25 hydroxyvitamin D (2...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01778-1

    authors: Zhou QF,Zhang MX,Tong SL,Tao RX,Hao JH,Huang K,Tao FB,Zhu P

    更新日期:2017-05-08 00:00:00

  • Publisher Correction: Association of PPM1G methylation with risk-taking in alcohol use disorder.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-67130-2

    authors: Park CI,Kim HW,Hwang SS,Kang JI,Kim SJ

    更新日期:2020-06-18 00:00:00

  • Do deposit-feeders compete? Isotopic niche analysis of an invasion in a species-poor system.

    abstract::Successful establishment of invasive species is often related to the existence of vacant niches. Competition occurs when invaders use the same limiting resources as members of the recipient community, which will be reflected in some overlap of their trophic niches. The concept of isotopic niche has been used to study ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep09715

    authors: Karlson AM,Gorokhova E,Elmgren R

    更新日期:2015-05-19 00:00:00

  • Pan-genome analysis and ancestral state reconstruction of class halobacteria: probability of a new super-order.

    abstract::Halobacteria, a class of Euryarchaeota are extremely halophilic archaea that can adapt to a wide range of salt concentration generally from 10% NaCl to saturated salt concentration of 32% NaCl. It consists of the orders: Halobacteriales, Haloferaciales and Natriabales. Pan-genome analysis of class Halobacteria was don...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77723-6

    authors: Gaba S,Kumari A,Medema M,Kaushik R

    更新日期:2020-12-03 00:00:00

  • Analysis of Bioactive Amino Acids from Fish Hydrolysates with a New Bioinformatic Intelligent System Approach.

    abstract::The current economics of the fish protein industry demand rapid, accurate and expressive prediction algorithms at every step of protein production especially with the challenge of global climate change. This help to predict and analyze functional and nutritional quality then consequently control food allergies in hype...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10890-1

    authors: Elaziz MA,Hemdan AM,Hassanien A,Oliva D,Xiong S

    更新日期:2017-09-07 00:00:00

  • Genomic Regions Associated With Interspecies Communication in Dogs Contain Genes Related to Human Social Disorders.

    abstract::Unlike their wolf ancestors, dogs have unique social skills for communicating and cooperating with humans. Previously, significant heritabilities for human-directed social behaviors have been found in laboratory beagles. Here, a Genome-Wide Association Study identified two genomic regions associated with dog's human-d...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep33439

    authors: Persson ME,Wright D,Roth LS,Batakis P,Jensen P

    更新日期:2016-09-29 00:00:00

  • Multi-band MIM refractive index biosensor based on Ag-air grating with equivalent circuit and T-matrix methods in near-infrared region.

    abstract::In this paper, a multi-band metal-insulator-metal (MIM) perfect absorber with refractive index sensing capability has been investigated in near-infrared region. The proposed structure has been studied for biomedical applications such as detection of solution of glucose in water, diagnosis of different stages of malari...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63459-w

    authors: Nejat M,Nozhat N

    更新日期:2020-04-14 00:00:00

  • Quality of non-expert citizen science data collected for habitat type conservation status assessment in Natura 2000 protected areas.

    abstract::EU biodiversity conservation policy is based on the Habitats Directive (92/43/EC), which aims that habitat types and species of Community interest should reach 'favourable conservation status'. To this end, Member States are obliged to perform periodic assessment of species and habitat conservation status through biod...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09316-9

    authors: Kallimanis AS,Panitsa M,Dimopoulos P

    更新日期:2017-08-21 00:00:00

  • Identification of a phosphinothricin-resistant mutant of rice glutamine synthetase using DNA shuffling.

    abstract::To date, only bar/pat gene derived from Streptomyces has been used to generate the commercial PPT-resistant crops currently available in the market. The limited source of bar/pat gene is probably what has caused the decrease in PPT-tolerance, which has become the main concern of those involved in field management prog...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15495

    authors: Tian YS,Xu J,Zhao W,Xing XJ,Fu XY,Peng RH,Yao QH

    更新日期:2015-10-23 00:00:00

  • Combining molecular typing and spatial pattern analysis to identify areas of high tuberculosis transmission in a moderate-incidence county in Taiwan.

    abstract::In total, 303 randomly selected clinical Mycobacterium tuberculosis (MTB) isolates from 303 patients (collected January to December 2012) in central Taiwan were examined. The major lineages found were Beijing (N = 114, 37.62%), Haarlem (N = 76, 25.08%) and East African-Indian (EAI) (N = 42, 13.86%). Notably, younger p...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05674-6

    authors: Chen YY,Chang JR,Wu CD,Yeh YP,Yang SJ,Hsu CH,Lin MC,Tsai CF,Lin MS,Su IJ,Dou HY

    更新日期:2017-07-14 00:00:00

  • Vibrio vulnificus VvhA induces autophagy-related cell death through the lipid raft-dependent c-Src/NOX signaling pathway.

    abstract::VvhA, a virulent factor of Vibrio (V.) vulnificus, induces acute cell death in a destructive manner. Autophagy plays an important role in cell death, but the functional role of VvhA in autophagy-related cell death has not been elucidated yet. We found that rVvhA significantly increased LC3 puncta formation and autopha...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27080

    authors: Song EJ,Lee SJ,Lim HS,Kim JS,Jang KK,Choi SH,Han HJ

    更新日期:2016-06-02 00:00:00

  • Mitochondrial CB1 receptor is involved in ACEA-induced protective effects on neurons and mitochondrial functions.

    abstract::Mitochondrial dysfunction contributes to cell death after cerebral ischemia/reperfusion (I/R) injury. Cannabinoid CB1 receptor is expressed in neuronal mitochondrial membranes (mtCB1R) and involved in regulating mitochondrial functions under physiological conditions. However, whether mtCB1R affords neuroprotection aga...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep12440

    authors: Ma L,Jia J,Niu W,Jiang T,Zhai Q,Yang L,Bai F,Wang Q,Xiong L

    更新日期:2015-07-28 00:00:00

  • Permeability Barrier and Microstructure of Skin Lipid Membrane Models of Impaired Glucosylceramide Processing.

    abstract::Ceramide (Cer) release from glucosylceramides (GlcCer) is critical for the formation of the skin permeability barrier. Changes in β-glucocerebrosidase (GlcCer'ase) activity lead to diminished Cer, GlcCer accumulation and structural defects in SC lipid lamellae; however, the molecular basis for this impairment is not c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06990-7

    authors: Sochorová M,Staňková K,Pullmannová P,Kováčik A,Zbytovská J,Vávrová K

    更新日期:2017-07-25 00:00:00

  • Social Environment Shapes the Speed of Cooperation.

    abstract::Are cooperative decisions typically made more quickly or slowly than non-cooperative decisions? While this question has attracted considerable attention in recent years, most research has focused on one-shot interactions. Yet it is repeated interactions that characterize most important real-world social interactions. ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29622

    authors: Nishi A,Christakis NA,Evans AM,O'Malley AJ,Rand DG

    更新日期:2016-07-20 00:00:00

  • Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii.

    abstract::A. baumannii infections are becoming more and more serious health issues with rapid emerging of multidrug and extremely drug resistant strains, and therefore, there is an urgent need for the development of nonantibiotic-based intervention strategies. This study aimed at identifying whether an outer membrane protein wi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep20724

    authors: Huang W,Yao Y,Wang S,Xia Y,Yang X,Long Q,Sun W,Liu C,Li Y,Chu X,Bai H,Yao Y,Ma Y

    更新日期:2016-02-08 00:00:00

  • A clinical investigation treating different types of fibroids identified by MRI-T2WI imaging with ultrasound guided high intensity focused ultrasound.

    abstract::Clinical data from 172 cases of uterine fibroids with different appearances on MRI-T2WI and accepted ultrasound guided high intensity focused ultrasound (USgHIFU) treatment were retrospectively analyzed. This study aimed to evaluate the clinical safety and efficacy of ablating different types of fibroids, classified b...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11486-5

    authors: Zhao WP,Zhang J,Han ZY,Yao JP,Zhou X,Liang P

    更新日期:2017-09-07 00:00:00

  • Assessment of post-harvest losses and carbon footprint in intensive lowland rice production in Myanmar.

    abstract::This paper examines how a move from traditional post-harvest operations of smallholder rice farms in the Ayeyarwaddy delta, Myanmar, to improved post-harvest operations affected income, energy efficiency and greenhouse gas emissions (GHGE). Harvest and post-harvest losses were investigated in a field experiment with 5...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-76639-5

    authors: Gummert M,Nguyen-Van-Hung,Cabardo C,Quilloy R,Aung YL,Thant AM,Kyaw MA,Labios R,Htwe NM,Singleton GR

    更新日期:2020-11-13 00:00:00

  • Overcoming sorafenib evasion in hepatocellular carcinoma using CXCR4-targeted nanoparticles to co-deliver MEK-inhibitors.

    abstract::Sorafenib is a RAF inhibitor approved for several cancers, including hepatocellular carcinoma (HCC). Inhibition of RAF kinases can induce a dose-dependent "paradoxical" upregulation of the downstream mitogen-activated protein kinase (MAPK) pathway in cancer cells. It is unknown whether "paradoxical" ERK activation occ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep44123

    authors: Chen Y,Liu YC,Sung YC,Ramjiawan RR,Lin TT,Chang CC,Jeng KS,Chang CF,Liu CH,Gao DY,Hsu FF,Duyverman AM,Kitahara S,Huang P,Dima S,Popescu I,Flaherty KT,Zhu AX,Bardeesy N,Jain RK,Benes CH,Duda DG

    更新日期:2017-03-09 00:00:00

  • Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity.

    abstract::The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Fl...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep41906

    authors: Kuhn BM,Nodzyński T,Errafi S,Bucher R,Gupta S,Aryal B,Dobrev P,Bigler L,Geisler M,Zažímalová E,Friml J,Ringli C

    更新日期:2017-02-06 00:00:00

  • Longitudinal micro-computed tomography-derived biomarkers quantify non-resolving lung fibrosis in a silicosis mouse model.

    abstract::In spite of many compounds identified as antifibrotic in preclinical studies, pulmonary fibrosis remains a life-threatening condition for which highly effective treatment is still lacking. Towards improving the success-rate of bench-to-bedside translation, we investigated in vivo µCT-derived biomarkers to repeatedly q...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73056-6

    authors: Dekoster K,Decaesteker T,Berghen N,Van den Broucke S,Jonckheere AC,Wouters J,Krouglov A,Lories R,De Langhe E,Hoet P,Verbeken E,Vanoirbeek J,Vande Velde G

    更新日期:2020-09-30 00:00:00

  • Low palaeoelevation of the northern Lhasa terrane during late Eocene: Fossil foraminifera and stable isotope evidence from the Gerze Basin.

    abstract::The Lhasa terrane is a key region for understanding the paleoelevation of the southern Tibetan Plateau after India-Asia collision. The Gerze Basin, located in the northern part of the Lhasa terrane, is a shortening-related basin. We discovered Lagena laevis (Bandy) fossils in upper Eocene strata of the Gerze Basin. Th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27508

    authors: Wei Y,Zhang K,Garzione CN,Xu Y,Song B,Ji J

    更新日期:2016-06-08 00:00:00

  • Necessity of p53-binding to the CDH1 locus for its expression defines two epithelial cell types differing in their integrity.

    abstract::TP53 mutation (i.e., loss of normal-p53) may evoke epithelial-mesenchymal transition (EMT), which was previously attributed to loss of certain miRNAs. However, not all epithelial cells undergo EMT upon TP53 mutation, and the p53-miRNA axis may not fully explain p53 function in epithelial integrity. We here show two mo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-20043-7

    authors: Oikawa T,Otsuka Y,Onodera Y,Horikawa M,Handa H,Hashimoto S,Suzuki Y,Sabe H

    更新日期:2018-01-25 00:00:00

  • Spatial Characterization of Tumor Perfusion Properties from 3D DCE-US Perfusion Maps are Early Predictors of Cancer Treatment Response.

    abstract::There is a need for noninvasive repeatable biomarkers to detect early cancer treatment response and spare non-responders unnecessary morbidities and costs. Here, we introduce three-dimensional (3D) dynamic contrast enhanced ultrasound (DCE-US) perfusion map characterization as inexpensive, bedside and longitudinal ind...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63810-1

    authors: El Kaffas A,Hoogi A,Zhou J,Durot I,Wang H,Rosenberg J,Tseng A,Sagreiya H,Akhbardeh A,Rubin DL,Kamaya A,Hristov D,Willmann JK

    更新日期:2020-04-24 00:00:00

  • Differential expression of genes in olive leaves and buds of ON- versus OFF-crop trees.

    abstract::Alternate bearing (AB) refers to the tendency of trees to have an irregular crop load from 1 year (ON) to the next year (OFF). Despite its economic importance, it is not fully understood how gene networks and their related metabolic pathways may influence the irregular bearing in olive trees. To unravel molecular mech...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72895-7

    authors: Dastkar E,Soleimani A,Jafary H,de Dios Alche J,Bahari A,Zeinalabedini M,Salami SA

    更新日期:2020-09-25 00:00:00