Abstract:
:RAS genotyping is mandatory to predict anti-EGFR monoclonal antibodies (mAbs) therapy resistance and BRAF genotyping is a relevant prognosis marker in patients with metastatic colorectal cancer. Although the role of hotspot mutations is well defined, the impact of uncommon mutations is still unknown. In this study, we aimed to discuss the potential utility of detecting uncommon RAS and BRAF mutation profiles with next-generation sequencing. A total of 779 FFPE samples from patients with metastatic colorectal cancer with valid NGS results were screened and 22 uncommon mutational profiles of KRAS, NRAS and BRAF genes were selected. In silico prediction of mutation impact was then assessed by 2 predictive scores and a structural protein modelling. Three samples carry a single KRAS non-hotspot mutation, one a single NRAS non-hotspot mutation, four a single BRAF non-hotspot mutation and fourteen carry several mutations. This in silico study shows that some non-hotspot RAS mutations seem to behave like hotspot mutations and warrant further examination to assess whether they should confer a resistance to anti-EGFR mAbs therapy for patients bearing these non-hotspot RAS mutations. For BRAF gene, non-V600E mutations may characterise a novel subtype of mCRC with better prognosis, potentially implying a modification of therapeutic strategy.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Franczak C,Kandathil SM,Gilson P,Husson M,Rouyer M,Demange J,Leroux A,Merlin JL,Harlé Adoi
10.1038/s41598-019-43646-0subject
Has Abstractpub_date
2019-05-08 00:00:00pages
7083issue
1issn
2045-2322pii
10.1038/s41598-019-43646-0journal_volume
9pub_type
杂志文章abstract::Our study focused on radiocaesium (137Cs) partitioning in forests, three vegetation periods after the Fukushima Daiichi nuclear power plant accident. 137Cs distribution in forest components (organic and mineral soil layers as well as tree compartments: stem, bark, needles, branches and roots) was measured for two Japa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37618
更新日期:2016-11-23 00:00:00
abstract::Broadly neutralizing antibodies developed from the IGHV1-69 germline gene are known to bind to the stem region of hemagglutinin in diverse influenza viruses but the sequence determinants for the antigen recognition, including neutralization potency and binding affinity, are not clearly understood. Such understanding c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15053
更新日期:2015-10-12 00:00:00
abstract::Despite recent advances in drug delivery, the targeted treatment of unhealthy cells or tissues continues to remain a priority. In cancer (much like other pathologies), delivery vectors are designed to exploit physical and biological features of unhealthy tissues that are not always homogenous across the disease. In so...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55909-x
更新日期:2020-01-13 00:00:00
abstract::Functional imaging has become an important tool in oncology because it not only provides information about the size and localization of the tumour, but also about the pathophysiological features of the tumoural cells. One of the characteristic features of some tumour types is that their fast growth leads to deficient ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43618-4
更新日期:2019-05-08 00:00:00
abstract::There is a growing interest concerning the relevance of salivary cortisone levels in stress-related research. However, studies investigating morning patterns and day-to-day variability of cortisone versus cortisol levels are lacking. Cortisol and cortisone analysis by liquid chromatography-tandem mass spectroscopy (LC...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55571-3
更新日期:2019-12-17 00:00:00
abstract::It has been suggested that an age-related loss of cognitive function might be driven by atherosclerotic effects associated with altered lipid patterns. However, the relationship between Lipoprotein (a) [Lp(a)] and healthy cognitive aging has not yet been sufficiently investigated. For the current analysis we used the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66783-3
更新日期:2020-06-30 00:00:00
abstract::We aimed to investigate whether the newborns of mothers with maternal depression (MD) had lower vitamin D levels than newborns of non-MD (NMD) mothers and identify the potential mechanism underlying this association. Maternal depressive symptoms in late pregnancy and concentrations of cord blood 25 hydroxyvitamin D (2...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01778-1
更新日期:2017-05-08 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-67130-2
更新日期:2020-06-18 00:00:00
abstract::Successful establishment of invasive species is often related to the existence of vacant niches. Competition occurs when invaders use the same limiting resources as members of the recipient community, which will be reflected in some overlap of their trophic niches. The concept of isotopic niche has been used to study ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09715
更新日期:2015-05-19 00:00:00
abstract::Halobacteria, a class of Euryarchaeota are extremely halophilic archaea that can adapt to a wide range of salt concentration generally from 10% NaCl to saturated salt concentration of 32% NaCl. It consists of the orders: Halobacteriales, Haloferaciales and Natriabales. Pan-genome analysis of class Halobacteria was don...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77723-6
更新日期:2020-12-03 00:00:00
abstract::The current economics of the fish protein industry demand rapid, accurate and expressive prediction algorithms at every step of protein production especially with the challenge of global climate change. This help to predict and analyze functional and nutritional quality then consequently control food allergies in hype...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10890-1
更新日期:2017-09-07 00:00:00
abstract::Unlike their wolf ancestors, dogs have unique social skills for communicating and cooperating with humans. Previously, significant heritabilities for human-directed social behaviors have been found in laboratory beagles. Here, a Genome-Wide Association Study identified two genomic regions associated with dog's human-d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33439
更新日期:2016-09-29 00:00:00
abstract::In this paper, a multi-band metal-insulator-metal (MIM) perfect absorber with refractive index sensing capability has been investigated in near-infrared region. The proposed structure has been studied for biomedical applications such as detection of solution of glucose in water, diagnosis of different stages of malari...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63459-w
更新日期:2020-04-14 00:00:00
abstract::EU biodiversity conservation policy is based on the Habitats Directive (92/43/EC), which aims that habitat types and species of Community interest should reach 'favourable conservation status'. To this end, Member States are obliged to perform periodic assessment of species and habitat conservation status through biod...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09316-9
更新日期:2017-08-21 00:00:00
abstract::To date, only bar/pat gene derived from Streptomyces has been used to generate the commercial PPT-resistant crops currently available in the market. The limited source of bar/pat gene is probably what has caused the decrease in PPT-tolerance, which has become the main concern of those involved in field management prog...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15495
更新日期:2015-10-23 00:00:00
abstract::In total, 303 randomly selected clinical Mycobacterium tuberculosis (MTB) isolates from 303 patients (collected January to December 2012) in central Taiwan were examined. The major lineages found were Beijing (N = 114, 37.62%), Haarlem (N = 76, 25.08%) and East African-Indian (EAI) (N = 42, 13.86%). Notably, younger p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05674-6
更新日期:2017-07-14 00:00:00
abstract::VvhA, a virulent factor of Vibrio (V.) vulnificus, induces acute cell death in a destructive manner. Autophagy plays an important role in cell death, but the functional role of VvhA in autophagy-related cell death has not been elucidated yet. We found that rVvhA significantly increased LC3 puncta formation and autopha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27080
更新日期:2016-06-02 00:00:00
abstract::Mitochondrial dysfunction contributes to cell death after cerebral ischemia/reperfusion (I/R) injury. Cannabinoid CB1 receptor is expressed in neuronal mitochondrial membranes (mtCB1R) and involved in regulating mitochondrial functions under physiological conditions. However, whether mtCB1R affords neuroprotection aga...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12440
更新日期:2015-07-28 00:00:00
abstract::Ceramide (Cer) release from glucosylceramides (GlcCer) is critical for the formation of the skin permeability barrier. Changes in β-glucocerebrosidase (GlcCer'ase) activity lead to diminished Cer, GlcCer accumulation and structural defects in SC lipid lamellae; however, the molecular basis for this impairment is not c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06990-7
更新日期:2017-07-25 00:00:00
abstract::Are cooperative decisions typically made more quickly or slowly than non-cooperative decisions? While this question has attracted considerable attention in recent years, most research has focused on one-shot interactions. Yet it is repeated interactions that characterize most important real-world social interactions. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29622
更新日期:2016-07-20 00:00:00
abstract::A. baumannii infections are becoming more and more serious health issues with rapid emerging of multidrug and extremely drug resistant strains, and therefore, there is an urgent need for the development of nonantibiotic-based intervention strategies. This study aimed at identifying whether an outer membrane protein wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20724
更新日期:2016-02-08 00:00:00
abstract::Clinical data from 172 cases of uterine fibroids with different appearances on MRI-T2WI and accepted ultrasound guided high intensity focused ultrasound (USgHIFU) treatment were retrospectively analyzed. This study aimed to evaluate the clinical safety and efficacy of ablating different types of fibroids, classified b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11486-5
更新日期:2017-09-07 00:00:00
abstract::This paper examines how a move from traditional post-harvest operations of smallholder rice farms in the Ayeyarwaddy delta, Myanmar, to improved post-harvest operations affected income, energy efficiency and greenhouse gas emissions (GHGE). Harvest and post-harvest losses were investigated in a field experiment with 5...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76639-5
更新日期:2020-11-13 00:00:00
abstract::Sorafenib is a RAF inhibitor approved for several cancers, including hepatocellular carcinoma (HCC). Inhibition of RAF kinases can induce a dose-dependent "paradoxical" upregulation of the downstream mitogen-activated protein kinase (MAPK) pathway in cancer cells. It is unknown whether "paradoxical" ERK activation occ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44123
更新日期:2017-03-09 00:00:00
abstract::The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Fl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41906
更新日期:2017-02-06 00:00:00
abstract::In spite of many compounds identified as antifibrotic in preclinical studies, pulmonary fibrosis remains a life-threatening condition for which highly effective treatment is still lacking. Towards improving the success-rate of bench-to-bedside translation, we investigated in vivo µCT-derived biomarkers to repeatedly q...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73056-6
更新日期:2020-09-30 00:00:00
abstract::The Lhasa terrane is a key region for understanding the paleoelevation of the southern Tibetan Plateau after India-Asia collision. The Gerze Basin, located in the northern part of the Lhasa terrane, is a shortening-related basin. We discovered Lagena laevis (Bandy) fossils in upper Eocene strata of the Gerze Basin. Th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27508
更新日期:2016-06-08 00:00:00
abstract::TP53 mutation (i.e., loss of normal-p53) may evoke epithelial-mesenchymal transition (EMT), which was previously attributed to loss of certain miRNAs. However, not all epithelial cells undergo EMT upon TP53 mutation, and the p53-miRNA axis may not fully explain p53 function in epithelial integrity. We here show two mo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20043-7
更新日期:2018-01-25 00:00:00
abstract::There is a need for noninvasive repeatable biomarkers to detect early cancer treatment response and spare non-responders unnecessary morbidities and costs. Here, we introduce three-dimensional (3D) dynamic contrast enhanced ultrasound (DCE-US) perfusion map characterization as inexpensive, bedside and longitudinal ind...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63810-1
更新日期:2020-04-24 00:00:00
abstract::Alternate bearing (AB) refers to the tendency of trees to have an irregular crop load from 1 year (ON) to the next year (OFF). Despite its economic importance, it is not fully understood how gene networks and their related metabolic pathways may influence the irregular bearing in olive trees. To unravel molecular mech...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72895-7
更新日期:2020-09-25 00:00:00