Abstract：
:We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wu B,Chen J,Singer RHdoi
10.1038/srep03615subject
Has Abstractpub_date
2014-01-09 00:00:00pages
3615issn
2045-2322pii
srep03615journal_volume
4pub_type
杂志文章abstract：:A large retreat of sea-ice in the 'stormy' Atlantic Sector of the Arctic Ocean has become evident through a series of record minima for the winter maximum sea-ice extent since 2015. Results from the Norwegian young sea ICE (N-ICE2015) expedition, a five-month-long (Jan-Jun) drifting ice station in first and second yea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45574-5
更新日期：2019-06-25 00:00:00
abstract：:Animals execute sensorimotor sequences to optimize performance of complex actions series. However, the sensory aspects of these sequences and their dynamic control are often poorly understood. We trained bats to fly to targets at different distances, and analysed their sensory behavior before and during flight to test...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00543-8
更新日期：2017-03-28 00:00:00
abstract：:The integration of dislocation-free Ge nano-islands was realized via selective molecular beam epitaxy on Si nano-tip patterned substrates. The Si-tip wafers feature a rectangular array of nanometer sized Si tips with (001) facet exposed among a SiO2 matrix. These wafers were fabricated by complementary metal-oxide-sem...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22709
更新日期：2016-03-04 00:00:00
abstract：:Whipple's disease is a systemic infectious disease associated with the bacterium Tropheryma whipplei. Numerous reports have presented puzzling discrepancies between diagnosis methods. We addressed this confusion using fluorescent in situ hybridization and immunofluorescence assays to evaluate 34 duodenal biopsies and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23883
更新日期：2016-03-30 00:00:00
abstract：:Organ transplantation remains the most effective treatment for patients with late stage organ failure. Transgenic pigs provide an alternative organ donor source to the limited availability of human organs. However, cellular rejection still remains to be the obstacle for xenotransplantation. Superior to other methods, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04322-3
更新日期：2017-06-21 00:00:00
abstract：:Exolysin (ExlA) is a recently-identified pore-forming toxin secreted by a subset of Pseudomonas aeruginosa strains identified worldwide and devoid of Type III secretion system (T3SS), a major virulence factor. Here, we characterized at the ultrastructural level the lesions caused by an ExlA-secreting strain, CLJ1, in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02349-0
更新日期：2017-05-18 00:00:00
abstract：:Consistent cell preparation is a fundamental preliminary step for understanding complex cellular mechanisms in various cell-based research fields, including basic cell biology, cancer research, and tissue engineering. However, certain elusive factors, such as cellular de-differentiation and contamination with mycoplas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02001-x
更新日期：2017-05-15 00:00:00
abstract：:Global climate change (GCC) can negatively affect freshwater ecosystems. However, the degree to which freshwater populations can acclimate to long-term warming and the underlying molecular mechanisms are not yet fully understood. We used the cooling water discharge (CWD) area of a power plant as a model for long-term ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39476
更新日期：2016-12-20 00:00:00
abstract：:We developed a solvothermal-induced self-assembly approach to construct three dimensional (3D) macroscopic Fe2O3 nanocubes/nitrogen-doped graphene (Fe2O3-NC/GN) aerogel as anode materials for lithium-ion batteries (LIBs). The Fe2O3 nanocubes with length of ~50 nm are homogeneously anchored on 3D GN frameworks and as s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07171
更新日期：2014-11-25 00:00:00
abstract：:This work presents a method of grouping the electron spinors and the phonon modes of metal oxide crystals such as vanadium dioxide into an SU(2) gauge theory. The gauge "charge" is the electron spin, which is assumed to couple to the transverse acoustic phonons on the basis of spin ordering phenomena in [Formula: see ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68958-4
更新日期：2020-07-27 00:00:00
abstract：:The expression of aminoglycoside-modifying enzymes represents a survival strategy of antibiotic-resistant bacteria. Aminoglycoside 2'-N-acetyltransferase [AAC(2')] neutralizes aminoglycoside drugs by acetylation of their 2' amino groups in an acetyl coenzyme A (CoA)-dependent manner. To understand the structural featu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78699-z
更新日期：2020-12-09 00:00:00
abstract：:We provide a unified and exact framework for the variance-based uncertainty relations. This unified framework not only recovers some well-known previous uncertainty relations, but also fixes the deficiencies of them. Utilizing the unified framework, we can construct the new uncertainty relations in both product and su...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56803-2
更新日期：2020-01-10 00:00:00
abstract：:In optics, when polychromatic light is filtered by an optical filter, the monochromaticity of the light can be improved. In this work, we reported that Ag dopant atoms could be used as an optical filter for nanosized Mn:ZnSe quantum dots (QDs). If no Ag doping, aqueous Mn:ZnSe QDs have low monochromaticity due to coex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14817
更新日期：2015-10-08 00:00:00
abstract：:When individuals breed more than once, parents are faced with the choice of whether to re-mate with their old partner or divorce and select a new mate. Evolutionary theory predicts that, following successful reproduction with a given partner, that partner should be retained for future reproduction. However, recent wor...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72521-6
更新日期：2020-09-23 00:00:00
abstract：:The paper presents new knowledge on primary defect formation in tungsten (W) and iron (Fe) irradiated by fission and high-energy neutrons at near-room temperature. Using a well-established method of positron-annihilation lifetime-spectroscopy (PALS), it was found that irradiation of W in the fission reactor and by hig...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75737-8
更新日期：2020-11-03 00:00:00
abstract：:In this paper we report for the first time an n-type carbon nanotube field effect transistor which is air- and water-stable, a necessary requirement for electrolyte gated CMOS circuit operation. The device is obtained through a simple process, where the native p-type transistor is converted to an n-type. This conversi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29882-w
更新日期：2018-07-30 00:00:00
abstract：:Experiments on the Ultimatum Game (UG) repeatedly show that people's behaviour is far from rational. In UG experiments, a subject proposes how to divide a pot and the other can accept or reject the proposal, in which case both lose everything. While rational people would offer and accept the minimum possible amount, i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19503-x
更新日期：2018-01-19 00:00:00
abstract：:Arthroplasty is a surgical procedure to restore the function of the joint of patient suffering from knee osteoarthritis. However, postoperative functional deficits are reported even after a rehabilitation program. In order to determine the origin of functional deficits of patient suffering from knee osteoarthritis and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62146-0
更新日期：2020-03-24 00:00:00
abstract：:Definitive evidence for the direct band gap predicted for Wurtzite Gallium Phosphide (WZ GaP) nanowires has remained elusive due to the lack of strong band-to-band luminescence in these materials. In order to circumvent this problem, we successfully obtained large volume WZ GaP structures grown by nanoparticle-crawlin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64809-4
更新日期：2020-05-13 00:00:00
abstract：:Tractography atlas-based analysis (TABS) is a new diffusion tensor image (DTI) statistical analysis method for detecting and understanding voxel-wise white matter properties along a fiber tract. An important requisite for accurate and sensitive TABS is the availability of a deformation field that is able to register D...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12965-5
更新日期：2017-10-04 00:00:00
abstract：:Kabuki Syndrome (KS) is a rare disorder characterized by distinctive facial features, short stature, skeletal abnormalities, and neurodevelopmental deficits. Previously, we showed that loss of function of RAP1A, a RAF1 regulator, can activate the RAS/MAPK pathway and cause KS, an observation recapitulated in other gen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28709-y
更新日期：2018-07-17 00:00:00
abstract：:Although hepatitis B virus (HBV) infection is the leading cause of liver fibrosis (LF), the mechanisms underlying liver fibrotic progression remain unclear. Here, we investigated the gene expression profiles of HBV-related LF patients. Whole genome expression arrays were used to detect gene expression in liver biopsy ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43446
更新日期：2017-03-06 00:00:00
abstract：:Multiplexed gene expression optimization via modulation of gene translation efficiency through ribosome binding site (RBS) engineering is a valuable approach for optimizing artificial properties in bacteria, ranging from genetic circuits to production pathways. Established algorithms design smart RBS-libraries based o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12395-3
更新日期：2017-09-26 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-44364-3
更新日期：2019-06-06 00:00:00
abstract：:Functional neuroimaging studies have implicated regions of both ventrolateral prefrontal cortex (VLPFC) and angular gyrus in processes associated with retrieving goal-relevant information, which increases the fidelity and richness of long-term memory (LTM). To further investigate the roles of these cortical regions as...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33164-w
更新日期：2018-10-05 00:00:00
abstract：:Associated with global warming, drought has destructive influences on agriculture and ecosystems, especially in the fragile Qinghai-Tibet Plateau (QTP). This study investigated spatial-temporal patterns of meteorological drought in the QTP and its surrounding areas and made an attempt to explore the relationship betwe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71295-1
更新日期：2020-08-31 00:00:00
abstract：:Heat shock factor-1 (HSF1) protects neurons from death caused by the accumulation of misfolded proteins by stimulating the transcription of genes encoding heat shock proteins (HSPs). This stimulatory action depends on the association of trimeric HSF1 to sequences within HSP gene promoters. However, we recently describ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35610-1
更新日期：2018-11-22 00:00:00
abstract：:Five-component icosahedral quasicrystals with compositions in the range Al68-73Fe11-16Cu10-12Cr1-4Ni1-2 were recently recovered after shocking metallic CuAl5 and (Mg0.75Fe0.25)2SiO4 olivine in a stainless steel 304 chamber, intended to replicate a natural shock that affected the Khatyrka meteorite. The iron in those q...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15771-1
更新日期：2017-11-15 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is one of the most common cause of malignancy-related mortality worldwide. It is urgently needed to develop potential drugs with good efficacy and low toxicity for HCC treatment. The anti-tumor effect of Traditional Chinese Medicine (TCM) has received increasing attention worldwide. Tram...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31298
更新日期：2016-08-09 00:00:00
abstract：:Here, we report results on systematic analysis of DNA substrate preferences of three N6-adenine β-class DNA methyltransferases that are part of the type II restriction-modification systems. The studied enzymes were: M.EcoVIII, M.HindIII and M.LlaCI, which although found in phylogenetically distant bacteria (γ-proteoba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26434-0
更新日期：2018-05-29 00:00:00
