Abstract:
:Organ transplantation remains the most effective treatment for patients with late stage organ failure. Transgenic pigs provide an alternative organ donor source to the limited availability of human organs. However, cellular rejection still remains to be the obstacle for xenotransplantation. Superior to other methods, antigen-specific regulatory T cells (Treg) alleviate cellular rejection with fewer side effects. Here we demonstrate the use of a fast method to provide tolerogenic dendritic cells (tolDC) that can be used to generate effective porcine-specific Treg cells (PSTreg). TolDC were produced within three days from human monocytes in medium supplemented with anti-inflammatory cytokines. Treg were generated from naïve CD4+ T cells and induced to become PSTreg by cocultivation with porcine-antigen-loaded tolDC. Results showed that PSTreg exhibited the expected phenotype, CD4+CD25+CD127low/- Foxp3+, and a more activated phenotype. The specificity of PSTreg was demonstrated by suppression of effector T cell (Teff) activation markers of different stages and inhibition of Teff cell proliferation. TolDC and PSTreg exhibited high expression of IL-10 and TGF-β1 at both protein and RNA levels, and PSTreg also highly expressed IL-35 at RNA levels. Upon restimulation, PSTreg retained the activated phenotype and specificity. Taken together, the newly developed procedure allows efficient generation of highly suppressive PSTreg.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Li M,Eckl J,Geiger C,Schendel DJ,Pohla Hdoi
10.1038/s41598-017-04322-3subject
Has Abstractpub_date
2017-06-21 00:00:00pages
3974issue
1issn
2045-2322pii
10.1038/s41598-017-04322-3journal_volume
7pub_type
杂志文章abstract::There is a growing appreciation for the diverse regulatory consequences of the family of proteins that bind to the secondary channel of E. coli RNA polymerase (RNAP), such as GreA, GreB or DksA. Similar binding sites could suggest a competition between them. GreA is characterised to rescue stalled RNAP complexes due t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73069-1
更新日期:2020-09-30 00:00:00
abstract::Metagenomics brings in new discoveries and insights into the uncultured microbial world. One fundamental task in metagenomics analysis is to determine the taxonomy of raw sequence fragments. Modern sequencing technologies produce relatively short fragments and greatly increase the number of fragments, and thus make th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09947-y
更新日期:2017-08-25 00:00:00
abstract::Proteasome inhibition represents an important anticancer strategy. Here, we studied the mechanisms at the basis of the pro-apoptotic activity of the standardized decoction of Hemidesmus indicus, a plant evoking a complex anticancer activity, and explored its inhibition of proteasome activity in human leukemia cells. A...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43609-5
更新日期:2019-05-10 00:00:00
abstract::In day-to-day life, humans usually perceive the location of sound sources as outside their heads. This externalized auditory spatial perception can be reproduced through headphones by recreating the sound pressure generated by the source at the listener's eardrums. This requires the acoustical features of the recordin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37342
更新日期:2016-11-17 00:00:00
abstract::Adaptive responses to hypoxia regulate hepatic lipid metabolism, but their consequences in nonalcoholic fatty liver disease (NAFLD) are largely unknown. Here, we show that hypoxia inducible factor-1 (HIF-1), a key determinant of hypoxic adaptations, prevents excessive hepatic lipid accumulation in the progression of N...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32586-w
更新日期:2018-09-21 00:00:00
abstract::The natural ligand 17β-estradiol (E2) is so far believed to induce a unique agonist-bound active conformation in the ligand binding domain (LBD) of the estrogen receptors (ERs). Both subtypes, ERα and ERβ, are transcriptionally activated in the presence of E2 with ERβ being somewhat less active than ERα under similar ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03774-x
更新日期:2017-06-14 00:00:00
abstract::During plaque progression, inflammatory cells progressively accumulate in the adventitia, paralleled by an increased presence of leaky vasa vasorum. We here show that next to vasa vasorum, also the adventitial lymphatic capillary bed is expanding during plaque development in humans and mouse models of atherosclerosis....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45263
更新日期:2017-03-28 00:00:00
abstract::Sorafenib is a multikinase inhibitor used as a first-line treatment for advanced hepatocellular carcinoma (HCC), but it has shown modest to low response rates. The characteristic tumour hypoxia of advanced HCC maybe a major factor underlying hypoxia-mediated treatment failure. Thus, it is urgent to elucidate the mecha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30483
更新日期:2016-08-01 00:00:00
abstract::Antibiogram profile of 1590 clinical bacterial isolates based on thirteen different antimicrobial compounds showed that 1.6% of the bacterial isolates are multidrug resistant. Distribution pattern based on 16S rRNA sequence analysis showed that Pseudomonas aeruginosa constituted the largest group (83.6%) followed by B...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23156
更新日期:2016-03-18 00:00:00
abstract::Tracking animal behavior by video is one of the most common tasks in the life sciences. Although commercial software exists for executing this task, they often present enormous cost to the researcher and can entail purchasing hardware that is expensive and lacks adaptability. Additionally, the underlying code is often...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56408-9
更新日期:2019-12-27 00:00:00
abstract::Anti-EGFR antibodies are effective in therapies for late-stage colorectal cancer (CRC); however, many tumours are unresponsive or develop resistance. We performed genomic analysis of intrinsic and acquired resistance to anti-EGFR therapy in prospectively collected tumour samples from 25 CRC patients receiving cetuxima...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51981-5
更新日期:2019-10-25 00:00:00
abstract::Limited reference values exist for visceral adipose tissue (VAT) mass measured by DXA. The objectives of this study were to provide reference values for DXA-derived VAT mass and compare the association with anthropometry measures. The study cohort comprised 677 men and 738 women aged 18-65 years from Western Australia...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73631-x
更新日期:2020-10-30 00:00:00
abstract::Widely regarded as an imminent threat to our oceans, ocean acidification has been documented in all oceanic basins. Projected changes in seawater chemistry will have catastrophic biotic effects due to ocean acidification hindering biogenic carbonate production, which will in turn lead to substantial changes in marine ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58501-w
更新日期:2020-01-31 00:00:00
abstract::Discovery of novel biomarkers is critical for early diagnosis of acute coronary syndrome (ACS). Serum metabolite profiling of ST-elevation myocardial infarction (STEMI), unstable angina (UA) and healthy controls was performed using gas chromatography mass spectrometry (GC/MS), solid-phase microextraction coupled to ga...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36359
更新日期:2016-11-08 00:00:00
abstract::Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00578-x
更新日期:2017-03-28 00:00:00
abstract::Microcalcifications are vital mammographic indicators contributing to the early detection of up to 50% of non-palpable tumours and may also be valuable as prognostic markers. However, the precise mechanism by which they form remains incompletely understood. Following development of an in vitro model using human breast...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36496-9
更新日期:2019-01-24 00:00:00
abstract::The relationship between obstructive sleep apnea (OSA) and chronic rhinosinusitis (CRS) still remains unclear. This retrospective cohort study aimed to investigate the relationship between OSA and subsequent CRS using a population-based dataset. The study used data from the Taiwan Longitudinal Health Insurance Databas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20786
更新日期:2016-02-10 00:00:00
abstract::Designing a new generation of energy-intensive and sustainable electrode materials for batteries to power a variety of applications is an imperative task. The use of biomaterials as a nanosized structural template for these materials has the potential to produce hitherto unachievable structures. In this report, we hav...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07736
更新日期:2015-01-13 00:00:00
abstract::The development of effective central nervous system (CNS) drugs has been hampered by the lack of robust strategies to mimic the blood-brain barrier (BBB) and cerebrovascular impairments in vitro. Recent technological advancements in BBB modeling using induced pluripotent stem cells (iPSCs) allowed to overcome some of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52213-6
更新日期:2019-11-05 00:00:00
abstract::A peptide vaccine designed to induce T-cell immunity to telomerase, GV1001, has been shown to modulate cellular signaling pathways and confer a direct anti-cancer effect through the interaction with heat shock protein (HSP) 90 and 70. Here, we have found that GV1001 can modulate transactivation protein-mediated human ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28896
更新日期:2016-07-01 00:00:00
abstract::Bragg coherent X-ray diffraction imaging (BCDI) has emerged as a powerful technique to image the local displacement field and strain in nanocrystals, in three dimensions with nanometric spatial resolution. However, BCDI relies on both dataset collection and phase retrieval algorithms that can induce artefacts in the r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53774-2
更新日期:2019-11-22 00:00:00
abstract::Extracellular vesicles (EVs) are membrane surrounded structures released by cells, which have been increasingly recognized as mediators of intercellular communication. Recent reports indicate that EVs participate in important biological processes and could serve as potential source for cancer biomarkers. As an attract...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24669
更新日期:2016-04-19 00:00:00
abstract::Application of controlled-release urea (CRU) improves crop yield and nitrogen use efficiency (NUE) compared with conventional urea. However, the effectiveness of CRU differs with fertilization placement. A two site-year field experiment was carried out in fluvo-aquic soil in central China to study the effects of two N...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56912-y
更新日期:2019-12-30 00:00:00
abstract::In this report we have evaluated radiation effective dose received by patients during ECG-gated CCTA examinations based on gender, heart rate, tube voltage protocol and body mass index (BMI). A total of 1,824 patients were retrospectively recruited (1,139 men and 685 women) and they were divided into Group 1 (CCTA wit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40758-5
更新日期:2019-03-13 00:00:00
abstract::Proper determination of agonist efficacy is essential in the assessment of agonist selectivity and signalling bias. Agonist efficacy is a relative term that is dependent on the system in which it is measured, especially being dependent on receptor expression level. The operational model (OM) of functional receptor ago...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40993-w
更新日期:2019-03-15 00:00:00
abstract::The concept of milk as a healthy food has opened the way for studies on milk components, from nutrients to microRNAs, molecules with broad regulatory properties present in large quantities in milk. Characterization of these components has been performed in several species, such as humans and bovine, depending on the s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56690-7
更新日期:2019-12-30 00:00:00
abstract::Understanding the human cytotoxic T lymphocyte (CTL) biology is crucial to develop novel strategies aiming at maximizing their lytic capacity against cancer cells. Here we introduce an agent-based model, calibrated on population-scale experimental data that allows quantifying human CTL per capita killing. Our model hi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48711-2
更新日期:2019-08-23 00:00:00
abstract::Understanding the excited-state dynamics in perovskite photovoltaics is necessary for progress in these materials, but changes in dynamics depending on the fabrication processes used for perovskite photoactive layers remain poorly characterised. Here we report a comparative study on femtosecond transient absorption (T...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16654-1
更新日期:2017-11-28 00:00:00
abstract::There has been a rapid increase in Danish pig herds testing positive for livestock-associated Methicillin-resistant Staphylococcus aureus (LA-MRSA) since the first screening in 2008. Despite a national action plan to control LA-MRSA in the Danish pig population, 88% of pig herds tested positive in a 2016 cross-section...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37075-8
更新日期:2019-01-24 00:00:00
abstract::Moisture in materials can be a source of future outgassing and exacerbate unwanted changes in physical and chemical properties. Here, we investigate the effect of sample size and shape on the moisture transport phenomena through a combined experimental and modeling approach. Several different materials varying in size...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74898-w
更新日期:2020-10-20 00:00:00