Abstract:
:The expression of aminoglycoside-modifying enzymes represents a survival strategy of antibiotic-resistant bacteria. Aminoglycoside 2'-N-acetyltransferase [AAC(2')] neutralizes aminoglycoside drugs by acetylation of their 2' amino groups in an acetyl coenzyme A (CoA)-dependent manner. To understand the structural features and molecular mechanism underlying AAC(2') activity, we overexpressed, purified, and crystallized AAC(2') from Mycolicibacterium smegmatis [AAC(2')-Id] and determined the crystal structures of its apo-form and ternary complexes with CoA and four different aminoglycosides (gentamicin, sisomicin, neomycin, and paromomycin). These AAC(2')-Id structures unraveled the binding modes of different aminoglycosides, explaining the broad substrate specificity of the enzyme. Comparative structural analysis showed that the α4-helix and β8-β9 loop region undergo major conformational changes upon CoA and substrate binding. Additionally, structural comparison between the present paromomycin-bound AAC(2')-Id structure and the previously reported paromomycin-bound AAC(6')-Ib and 30S ribosome structures revealed the structural features of paromomycin that are responsible for its antibiotic activity and AAC binding. Taken together, these results provide useful information for designing AAC(2') inhibitors and for the chemical modification of aminoglycosides.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Jeong CS,Hwang J,Do H,Cha SS,Oh TJ,Kim HJ,Park HH,Lee JHdoi
10.1038/s41598-020-78699-zsubject
Has Abstractpub_date
2020-12-09 00:00:00pages
21503issue
1issn
2045-2322pii
10.1038/s41598-020-78699-zjournal_volume
10pub_type
杂志文章abstract::The knowledge of diffusion processes in semiconducting alloys is very important both technologically and from a theoretical point of view. Here we show that, self-diffusion in Si1-x Ge x alloys as a function of temperature and Ge concentration can be described by the cBΩ thermodynamic model. This model connects the ac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01301-6
更新日期:2017-05-02 00:00:00
abstract::Wetting phenomena in multi-phase systems govern the shape of the contact line which separates the different phases. For liquids in contact with solid surfaces wetting is typically described in terms of contact angle. While in macroscopic systems the contact angle can be determined experimentally, on the molecular scal...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26111
更新日期:2016-05-17 00:00:00
abstract::The effect on post-operative outcomes after coronary artery bypass graft (CABG) surgery is not clear. Among 17,812 patients who underwent CABG during October 1,2006-September 28,2012 in any Department of US Veterans Affairs (VA) hospital, we identified 5,968 with available preoperative urine albumin-creatinine ratio (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16458
更新日期:2015-11-09 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-50846-1
更新日期:2019-10-18 00:00:00
abstract::Inhibitors of cyclin-dependent kinases (ICKs) are key regulators of cyclin-dependent kinase activities and cell division. Herein, we identified eight ICKs in maize, which we named Zeama;ICKs (ZmICKs). Primary sequencing and phylogenetic analyses were used to divide the ZmICK family into two classes: group B and group ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43818
更新日期:2017-03-06 00:00:00
abstract::We derive the stationary probability distribution for a non-equilibrium system composed by an arbitrary number of degrees of freedom that are subject to Gaussian colored noise and a conservative potential. This is based on a multidimensional version of the Unified Colored Noise Approximation. By comparing theory with ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10742
更新日期:2015-05-29 00:00:00
abstract::Cells harboring latent HIV-1 pose a major obstacle to eradication of the virus. The 'shock and kill' strategy has been broadly explored to purge the latent reservoir; however, none of the current latency-reversing agents (LRAs) can safely and effectively activate the latent virus in patients. In this study, we report ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07157-0
更新日期:2017-08-25 00:00:00
abstract::Voltage-gated sodium channels (VGSCs) play a vital role in controlling neuronal excitability. Nav1.6 is the most abundantly expressed VGSCs subtype in the adult central nervous system and has been found to contribute to facilitate the hyperexcitability of neurons after electrical induction of status epilepticus (SE). ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38108
更新日期:2016-12-01 00:00:00
abstract::We are reporting a 3D printable composite paste having strong thixotropic rheology. The composite has been designed and investigated with highly conductive silver nanowires. The optimized electrical percolation threshold from both simulation and experiment is shown from 0.7 vol. % of silver nanowires which is signific...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03365-w
更新日期:2017-06-12 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-60514-4
更新日期:2020-02-19 00:00:00
abstract::Insulin-like growth factor-I (IGF-I) is involved in the maturation and maintenance of neurons, and impaired IGF-I signaling has been shown to play a role in various neurological diseases including stroke. The aim of the present study was to investigate the efficacy of an optimized IGF-I variant by adding a 40 kDa poly...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00336-z
更新日期:2017-03-21 00:00:00
abstract::The Ebola filovirus causes severe hemorrhagic fever with a high fatality rate in humans. The primary structural matrix protein VP40 displays transformer-protein characteristics and exists in different conformational and oligomeric states. VP40 plays crucial roles in viral assembly and budding at the plasma membrane of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28077-7
更新日期:2018-06-27 00:00:00
abstract::We present compiled geochemical data of young (mostly Pliocene-present) intermediate magmatic rocks from continental collisional belts and correlations between their whole-rock Sr/Y and La/Yb ratios and modern crustal thickness. These correlations, which are similar to those obtained from subduction-related magmatic a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07849-7
更新日期:2017-08-01 00:00:00
abstract::We hypothesized that beyond the Thalassophryne nattereri venoms ability to induce in mice a strong specific-Th2 response with high levels of specific IgE/IgG1, it would be able to trigger anaphylaxis in sensitized individuals. To investigate whether the venom is capable of inducing an allergic reaction in mice and cha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-57231-y
更新日期:2020-01-17 00:00:00
abstract::Epitope characterization is critical for elucidating the mechanism of action of drug candidates. However, traditional high-resolution epitope mapping techniques are not well suited for screening numerous drug candidates recognizing a similar target. Here, we use Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41179-0
更新日期:2019-03-18 00:00:00
abstract::Tolerance to Dutch elm disease (DED) has been linked to the rapid and/or high induction of disease-responsive genes after infection with the fungus Ophiostoma novo-ulmi. Although the fungal infection by O. novo-ulmi primarily takes places in xylem vessels, it is still unclear how xylem contributes to the defense again...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07779-4
更新日期:2017-08-02 00:00:00
abstract::SNPs, abundant in human genome with lower mutation rate, are attractive to genetic application like forensic, anthropological and evolutionary studies. Universal SNPs showing little allelic frequency variation among populations while remaining highly informative for human identification were obtained from previous stu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18683
更新日期:2015-12-22 00:00:00
abstract::Studies suggest that chromium deficiency is associated with elevated levels of fasting blood glucose, circulating insulin, cholesterol and triglycerides, and decreased proportion of lean body mass. However, data directly relating chromium levels to metabolic syndrome (MetS) risk are lacking. A total of 3,648 American ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15606
更新日期:2015-10-22 00:00:00
abstract::A potted experiment with Populus × euramericana 'Neva' was carried out to assess whether there are positive effects of magnetic treatment of saline water (MTSW) on nitrogen metabolism under controlled conditions in a greenhouse. Growth properties, nitrogen contents, enzyme activities and metabolite concentrations were...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45719-6
更新日期:2019-07-11 00:00:00
abstract::The hypothalamic-pituitary-adrenal (HPA) axis regulates responses to internal and external stressors. Many patients diagnosed with conditions such as depression or anxiety also have hyperactivity of the HPA axis. Hyper-stimulation of the HPA axis results in sustained elevated levels of glucocorticoids which impair neu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39867-y
更新日期:2019-03-01 00:00:00
abstract::To what extent deep-time dispersal limitation shapes present-day biodiversity at broad spatial scales remains elusive. Here, we compiled a continental dataset on the distributions of African lizard species in the reptile subfamily Agaminae (a relatively young, Neogene radiation of agamid lizards which ancestors coloni...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34014
更新日期:2016-09-27 00:00:00
abstract::A chronic antigenic stimulation is believed to sustain the leukemogenic development of chronic lymphocytic leukemia (CLL) and most of lymphoproliferative malignancies developed from mature B cells. Reproducing a proliferative stimulation ex vivo is critical to decipher the mechanisms of leukemogenesis in these maligna...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36853-8
更新日期:2019-01-24 00:00:00
abstract::Many cardiac pathologies involve changes in tissue structure. Conventional analysis of structural features is extremely time-consuming and subject to observer bias. The possibility to determine spatial interrelations between these features is often not fully exploited. We developed a staining protocol and an ImageJ-ba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76932-3
更新日期:2020-11-18 00:00:00
abstract::Periventricular nodular heterotopia (PNH) is a neural migration disorder which often presents clinically with seizures. However, the underlying functional neural basis of PNH is still unclear. We aimed to explore the underlying pathological mechanism of PNH by combining both whole brain functional connectivity (FC) an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55002-3
更新日期:2019-12-05 00:00:00
abstract::MicroRNA-200 (miR-200) family is highly expressed in ovarian cancer. We evaluated the levels of family members relative to the internal control miR-103a in ovarian cancer and control blood specimens collected from American and Hong Kong Chinese institutions, as well as from a laying hen spontaneous ovarian cancer mode...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77068-0
更新日期:2020-11-18 00:00:00
abstract::Disordered proteins are highly prevalent in biological systems, they control myriad signaling and regulatory processes, and their levels and/or cellular localization are often altered in human disease. In contrast to folded proteins, disordered proteins, due to conformational heterogeneity and dynamics, are not consid...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15686
更新日期:2015-10-28 00:00:00
abstract::Many molecular system biology approaches recognize various interactions and functional associations of proteins that occur in cellular processing. Further understanding of the characterization technique reveals noteworthy information. These types of known and predicted interactions, gained through multiple resources, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62508-8
更新日期:2020-03-27 00:00:00
abstract::The natural alkaloid berberine has several pharmacological properties and recently received attention as a potential anticancer agent. In this work, we investigated the molecular mechanisms underlying the anti-tumor effect of berberine on glioblastoma U343 and pancreatic carcinoma MIA PaCa-2 cells. Human dermal fibrob...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28952-3
更新日期:2018-07-13 00:00:00
abstract::Methane concentration in caves is commonly much lower than the external atmosphere, yet the cave CH4 depletion causal mechanism is contested and dynamic links to external diurnal and seasonal temperature cycles unknown. Here, we report a continuous 3-year record of cave methane and other trace gases in Jenolan Caves, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07769-6
更新日期:2017-08-16 00:00:00
abstract::Recent parallel studies clearly indicated that Merkel cells and the mechanosensitive piezo2 ion channel play critical roles in the light-touch somatosensation. Moreover, piezo2 was suggested to be a light-touch sensing ion channel without a role in pain sensing in mammals. However, biophysical characteristics of piezo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42492-4
更新日期:2019-04-23 00:00:00