Abstract：
:Milk-derived exosomes were identified as a novel mechanism of mother-to-child transmission of regulatory molecules, but their functions in intestinal tissues of neonates are not well-studied. Here, we characterized potential roles of porcine milk-derived exosomes in the intestinal tract. In vitro, treatment with milk-derived exosomes (27 ± 3 ng and 55 ± 5 ng total RNA) significantly promoted IPEC-J2 cell proliferation by MTT, CCK8, EdU fluorescence and EdU flow cytometry assays. The qRT-PCR and Western blot analyses indicated milk-derived exosomes (0.27 ± 0.03 μg total RNA) significantly promoted expression of CDX2, IGF-1R and PCNA, and inhibited p53 gene expression involved in intestinal proliferation. Additionally, six detected miRNAs were significantly increased in IPEC-J2 cell, while FAS and SERPINE were significantly down-regulated relative to that in control. In vivo, treated groups (0.125 μg and 0.25 μg total RNA) significantly raised mice' villus height, crypt depth and ratio of villus length to crypt depth of intestinal tissues, significantly increased CDX2, PCNA and IGF-1R' expression and significantly inhibited p53' expression. Our study demonstrated that milk-derived exosomes can facilitate intestinal cell proliferation and intestinal tract development, thus giving a new insight for milk nutrition and newborn development and health.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Chen T,Xie MY,Sun JJ,Ye RS,Cheng X,Sun RP,Wei LM,Li M,Lin DL,Jiang QY,Xi QY,Zhang YLdoi
10.1038/srep33862subject
Has Abstractpub_date
2016-09-20 00:00:00pages
33862issn
2045-2322pii
srep33862journal_volume
6pub_type
杂志文章abstract：:The aim of this study was to assess the liver stiffness values in children with obesity versus healthy children on 2D-SWE and TE taking into account different laboratory parameters. We performed a case-control study on 287 children aged between 3 to 18 years, admitted in a Romanian Pediatric Tertiary Hospital, which w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56358-2
更新日期：2019-12-24 00:00:00
abstract：:Geographic variation in aridity determines environmental productivity patterns, including large-scale variability in pathogens, vectors and associated diseases. If disease risk decreases with increasing aridity and is matched by immune defense, we predict a decrease in innate immune function along a gradient of increa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62806-1
更新日期：2020-04-03 00:00:00
abstract：:Small for gestational age (SGA) is an established risk factor for cerebral palsy (CP) in term infants. However, there is conflicting data on the association between SGA and CP in moderate to late preterm infants. The aim of the article was to explore the relationship between SGA and CP in the moderate to late preterm ...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析,评审
doi：10.1038/srep38853
更新日期：2016-12-13 00:00:00
abstract：:Tomatoes are known to have ameliorative effects on cardiovascular disease and cancer. The nutritional value of tomatoes can be enhanced by increasing flavonoids content through genetic modification. The regulatory gene PAP1 (production of anthocyanin pigment 1) from Arabidopsis is reported to increase initial flavonoi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12355-x
更新日期：2017-09-28 00:00:00
abstract：:Epilepsy is a widespread neurological disease characterized by abnormal neuronal activity resulting in recurrent seizures. There is mounting evidence that a circadian system disruption, involving clock genes and their downstream transcriptional regulators, is associated with epilepsy. In this study, we characterized t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60638-7
更新日期：2020-02-28 00:00:00
abstract：:Relocation of earthquakes recorded by the agency for meteorology, climatology and geophysics (BMKG) in Indonesia and inversions of global positioning system (GPS) data reveal clear seismic gaps to the south of the island of Java. These gaps may be related to potential sources of future megathrust earthquakes in the re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72142-z
更新日期：2020-09-17 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-58429-1
更新日期：2020-01-27 00:00:00
abstract：:The ability to arrange cells and/or microparticles into the desired pattern is critical in biological, chemical, and metamaterial studies and other applications. Researchers have developed a variety of patterning techniques, which either have a limited capacity to simultaneously trap massive particles or lack the spat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14802-1
更新日期：2017-10-30 00:00:00
abstract：:Kaposiform hemangioendothelioma (KHE) is a relatively rare vascular tumor with an aggressive and infiltrating nature. Previous studies have revealed an exclusive relationship between KHE and Kasabach-Merritt Phenomenon (KMP), which is associated with high morbidity and mortality. No universally accepted treatment moda...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep36261
更新日期：2016-10-31 00:00:00
abstract：:A main controversy in cancer research is whether metastatic abilities are present in the most advanced clone of the primary tumor or result from independently acquired aberrations in early disseminated cancer cells as suggested by the linear and the parallel progression models, respectively. The genetic concordance be...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43813
更新日期：2017-03-09 00:00:00
abstract：:Magnesium has been shown to effectively prevent vascular calcification associated with chronic kidney disease. Magnesium has been hypothesized to prevent the upregulation of osteoblastic genes that potentially drives calcification. However, extracellular effects of magnesium on hydroxyapatite formation are largely neg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20241-3
更新日期：2018-02-01 00:00:00
abstract：:This is a systematical report on the potential health risk of heavy metals from the leather industries in Pakistan based on multiple biological matrices of the exposed workers and indoor dust samples. The adverse impacts of heavy metals on the oxidative enzyme and their risks to workers' health were also explored. Our...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09075-7
更新日期：2017-08-18 00:00:00
abstract：:Iron oxide nanoparticles (IONPs) are known to induce cytotoxicity in various cancer cell lines through the generation of reactive oxygen species (ROS). However, the studies on its potential to induce toxicity in normal cell lines and in vivo system are limited and ambiguity still exists. Additionally, small molecules ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43188-5
更新日期：2019-05-06 00:00:00
abstract：:To investigate the applicability of compound specific stable carbon isotope ratios (δ13C) of organics in assessment of their photochemical aging in the atmosphere, batch UV irradiation experiments were conducted on two ambient (anthropogenic and biogenic) aerosol samples in aqueous phase for 0.5-120 h. The irradiated ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36467
更新日期：2016-11-04 00:00:00
abstract：:We report a theoretical and experimental study of the aggregation kinetics of oppositely charged nanoparticles. Kinetic Monte Carlo simulations are performed for symmetric, charge-asymmetric and size-asymmetric systems of oppositely charged nanoparticles. Simulation results show that both the weight and number average...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40379-y
更新日期：2019-03-06 00:00:00
abstract：:Non-compressible hemorrhage is the most common preventable cause of death on battlefield and in civilian traumatic injuries. We report the use of microsecond pulses of electric current to induce rapid constriction in femoral and mesenteric arteries and veins in rats. Electrically-induced vasoconstriction could be indu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02111
更新日期：2013-01-01 00:00:00
abstract：:Within the last fifteen years, network theory has been successfully applied both to natural sciences and to socioeconomic disciplines. In particular, bipartite networks have been recognized to provide a particularly insightful representation of many systems, ranging from mutualistic networks in ecology to trade networ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10595
更新日期：2015-06-01 00:00:00
abstract：:Interleukin-27 (IL-27) is an important cytokine in inflammatory diseases, including coronary artery disease (CAD). To explore the precise role of IL-27 in CAD, we investigated the genetic association between IL27 and CAD in the GeneID Chinese Han population. A two-stage case control association analysis was performed ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25782
更新日期：2016-05-12 00:00:00
abstract：:Despite improvements in systemic therapy options for renal cancer, it remains one of the most drug-resistant malignancies. Interestingly, reports have shown that kahweol and cafestol, natural diterpenes extracted from coffee beans, exhibit anti-cancer activity. However, the multiple potential pharmacological actions o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80302-4
更新日期：2021-01-12 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-42527-w
更新日期：2019-04-12 00:00:00
abstract：:Proteins are of interest in nano-bio electronic devices due to their versatile structures, exquisite functionality and specificity. However, quantum transport measurements produce conflicting results due to technical limitations whereby it is difficult to precisely determine molecular orientation, the nature of the mo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39792
更新日期：2017-01-04 00:00:00
abstract：:The capacity of healthy adult listeners to accommodate to altered spectral cues to the source locations of broadband sounds has now been well documented. In recent years we have demonstrated that the degree and speed of accommodation are improved by using an integrated sensory-motor training protocol under anechoic co...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11981-9
更新日期：2017-09-14 00:00:00
abstract：:Mixed strain infections of Mycobacterium tuberculosis make diagnosis, treatment, and control of tuberculosis (TB) more difficult. This study was aimed to evaluate the relationship between mixed infections, antibiotic resistance patterns and treatment of TB patients. In this study, among 2850 suspected TB clinical samp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41410
更新日期：2017-01-25 00:00:00
abstract：:Epilepsy is one of the most common neurological disorders and the administration of antiepileptic drugs (AEDs) is the most common treatment. Although there are more than 15 AEDs available, a third of epilepsy patients remain refractory to available drugs, so novel effective drugs are needed. Here, we found that DV21, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01734-z
更新日期：2017-05-10 00:00:00
abstract：:The moist savanna zone covers 0.5 × 106 km2 in West Africa and is characterized by very low soil N levels limiting primary production, but the ecology of nitrifiers in these (agro)ecosystems is largely unknown. We compared the effects of six agricultural practices on nitrifier activity, abundance and diversity at nine...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10185-5
更新日期：2017-08-31 00:00:00
abstract：:Studies on the transcriptional control of gene expression play an important role in many areas of biology. Reference genes, which are often referred to as housekeeping genes, such as GAPDH, G3PDH, EF2, RpL7A, RpL10, TUBα and Actin, have traditionally been assumed to be stably expressed in all conditions, and they are ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08630-6
更新日期：2017-08-21 00:00:00
abstract：:Treatment of both-column fractures with posterior wall involvement is still a controversial topic. This type of posterior wall fracture is different from isolated acetabular posterior wall fracture (AO/OTA62-A1). The aim of this study is to compare the morphology of the posterior wall fragments of these two fracture p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56838-5
更新日期：2019-12-27 00:00:00
abstract：:Interferon (IFN)-γ is the major mediator of anti-tumor immune responses; nevertheless, cancer cells use intrigue strategies to alter IFN-γ signaling and avoid elimination. Understanding the immune regulatory mechanisms employed by acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) cells upon exposure to I...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48256-4
更新日期：2019-08-12 00:00:00
abstract：:Numerous studies have been conducted to examine the molecular mechanism of Haemophilus parasuis resistance to antibiotic, but rarely to tildipirosin. In the current study, transcriptional profiling was applied to analyse the variation in gene expression of JS0135 and tildipirosin-resistant JS32. The growth curves show...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07972-5
更新日期：2017-08-08 00:00:00
abstract：:The aim of the present study was to evaluate the predictive value of 25-hydroxyvitamin D, calcium and parathormone level, co-existence of thyroid nodule, thyroidectomy history and adenoma volume on the success of neck ultrasound (US) and technetium-99m sestamibi (MIBI) scan in primary hyperparathyroidism (PHP) patient...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48536-z
更新日期：2019-08-19 00:00:00
