Abstract:
:Studies on the transcriptional control of gene expression play an important role in many areas of biology. Reference genes, which are often referred to as housekeeping genes, such as GAPDH, G3PDH, EF2, RpL7A, RpL10, TUBα and Actin, have traditionally been assumed to be stably expressed in all conditions, and they are frequently used to normalize mRNA levels between different samples in qPCR analysis. However, it is known that the expression of these genes is influenced by numerous factors, such as experimental conditions. The difference in gene expression underlies a range of biological processes, including development, reproduction and behavior. The aim of this study was to show the problems associated with using reference genes in the qPCR technique, in a study on inbred strains of Spodoptera exigua selected toward cadmium resistance. We present and discuss our results and observations, and give some recommendations concerning the use and limitations of housekeeping genes as internal standards, especially in research on insects. Our results suggest that holometabolism and poikilothermia, as well as time since metamorphosis and the level of exposure to the selective factor (cadmium in this case), have a significant effect on the expression of reference genes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Płachetka-Bożek A,Augustyniak Mdoi
10.1038/s41598-017-08630-6subject
Has Abstractpub_date
2017-08-21 00:00:00pages
8338issue
1issn
2045-2322pii
10.1038/s41598-017-08630-6journal_volume
7pub_type
杂志文章abstract::Baited remote underwater video cameras were deployed in the Eastern Canadian Arctic, for the purpose of estimating local densities of the long-lived Greenland shark within five deep-water, data-poor regions of interest for fisheries development and marine conservation in Nunavut, Canada. A total of 31 camera deploymen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-19115-x
更新日期:2018-01-17 00:00:00
abstract::Alzheimer's disease (AD) is characterized by excessive production and deposition of amyloid-beta (Aβ) proteins as well as synapse dysfunction and loss. While soluble Aβ oligomers (AβOs) have deleterious effects on synapse function and reduce synapse number, the underlying molecular mechanisms are not well understood. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42548
更新日期:2017-02-13 00:00:00
abstract::Development of cost-effective counter electrode (CE) materials is a key issue for practical applications of photoelectrochemical solar energy conversion. Kesterite Cu2ZnSnS4 (CZTS) has been recognized as a potential CE material, but its electrocatalytic activity is still insufficient for the recovery of I-/I3- electro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26770-1
更新日期:2018-06-07 00:00:00
abstract::The feedback between dyke and sill intrusions and the evolution of stresses within volcanic systems is poorly understood, despite its importance for magma transport and volcano instability. Long-lived ocean island volcanoes are crosscut by thousands of dykes, which must be accommodated through a combination of flank s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74361-w
更新日期:2020-10-15 00:00:00
abstract::We reveal an outstanding potential of water as an inexpensive, abundant and bio-friendly high-refractive-index material for creating tunable all-dielectric photonic structures and metamaterials. Specifically, we demonstrate thermal, mechanical and gravitational tunability of magnetic and electric resonances in a metam...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13535
更新日期:2015-08-27 00:00:00
abstract::The pathophysiology of diabetic nephropathy (DN) in type 2 diabetes (T2D) patients is minimally understood. We compared untargeted high-resolution accurate mass (HRAM) orbitrap-based plasma metabolomic profiles of 31 T2D-DN (with estimated glomerular filtration rate ≤80 mL/min/1.73 m2), 29 T2D and 30 normal glucose to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54584-2
更新日期:2019-12-02 00:00:00
abstract::Hepatitis E virus is a common hepatotropic virus that causes serious gastrointestinal symptoms. Data of reported HEV cases in Zhejiang Province was collected between 2007 and 2012. Descriptive epidemiological methods and spatial-temporal epidemiological methods were used to investigate the epidemiological trends and i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25407
更新日期:2016-05-05 00:00:00
abstract::The authors present the preparation procedure and a computational model of a three-layered fibrous scaffold for prolonged drug release. The scaffold, produced by emulsion/sequential electrospinning, consists of a poly(D,L-lactic-co-glycolic acid) (PLGA) fiber layer sandwiched between two poly(ε-caprolactone) (PCL) lay...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68117-9
更新日期:2020-07-07 00:00:00
abstract::Type-II Diabetes Mellitus (T2DM) is one of the fastest growing public health issues of modern times, consuming 12% of worldwide health budgets and affecting an estimated 400 million people. A key pathological trait associated with this disease is the failure of normal glucose-stimulated insulin secretion (GSIS) from p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39681-6
更新日期:2019-02-27 00:00:00
abstract::Mycoplasma pneumoniae infection is conventionally diagnosed using serum antibody testing, microbial culture, and genetic testing. Recently, immunochromatography-based rapid mycoplasma antigen test kits have been developed and commercialised for rapid diagnosis of M. pneumoniae infection. However, as these kits do not ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19734-y
更新日期:2018-01-23 00:00:00
abstract::Transjugular intrahepatic portosystemic shunt (TIPS) is an effective therapy for reducing portal pressure. Hepatic myelopathy (HM), a rare complication of chronic liver diseases, remains obscure in terms of treatment and prognosis. We aimed to determine an optimal treat strategy for patients with HM after TIPS. Twenty...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究
doi:10.1038/s41598-018-33216-1
更新日期:2018-10-12 00:00:00
abstract::A significant advantage of a graphene biosensor is that it inherently represents a continuum of independent and aligned sensor-units. We demonstrate a nanoscale version of a micro-physiometer - a device that measures cellular metabolic activity from the local acidification rate. Graphene functions as a matrix of indep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06865
更新日期:2014-10-31 00:00:00
abstract::Current saliva testing methods rely on cutting edge yet expensive techniques for the detection and analysis of genetic material, proteins and biomarkers for clinical use. However, these techniques are limited in scope and often cannot be used with complex food materials. We propose an efficient ex-vivo tool for evalua...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31625-w
更新日期:2018-09-06 00:00:00
abstract::Accumulating evidence have shown the association of Parkinson's disease (PD) with osteoporosis. Bone loss in PD patients, considered to be multifactorial and a result of motor disfunction, is a hallmark symptom that causes immobility and decreased muscle strength, as well as malnutrition and medication. However, no kn...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50336-4
更新日期:2019-09-24 00:00:00
abstract::Circadian clocks keep time via ~ 24 h transcriptional feedback loops. In Drosophila, CLOCK-CYCLE (CLK-CYC) activators and PERIOD-TIMELESS (PER-TIM) repressors are feedback loop components whose transcriptional status varies over a circadian cycle. Although changes in the state of activators and repressors has been cha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75009-5
更新日期:2020-10-21 00:00:00
abstract::One of the hallmarks of cancers is their ability to develop resistance against therapeutic agents. Therefore, developing effective in vitro strategies to identify drug resistance remains of paramount importance for successful treatment. One of the ways cancer cells achieve drug resistance is through the expression of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56273-6
更新日期:2019-12-27 00:00:00
abstract::Release of pro-inflammatory mediators by mast cells is a key feature of allergic disease. The 'dogma' is that IgE molecules merely sensitise mast cells by binding FcεRI prior to cross-linking by multivalent allergen, receptor aggregation and mast cell activation. However, certain monoclonal IgE antibodies have been sh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09538
更新日期:2015-04-20 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-57570-1
更新日期:2020-01-14 00:00:00
abstract::Brain-computer interface (BCI) systems having the ability to classify brain waves with greater accuracy are highly desirable. To this end, a number of techniques have been proposed aiming to be able to classify brain waves with high accuracy. However, the ability to classify brain waves and its implementation in real-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45605-1
更新日期:2019-06-24 00:00:00
abstract::Elevated plasma homocysteine (Hcy) is suggested as an independent risk factor for stroke. We aimed to investigate the association of Hcy concentration with intracranial atherosclerosis (ICAS) and extracranial AS (ECAS) in hypertensive patients without stroke in Chinese population and to explore modified effect of meth...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-19125-9
更新日期:2018-01-12 00:00:00
abstract::Cellular reprogramming involves profound alterations in genome-wide gene expression that is precisely controlled by a hypothetical epigenetic code. Small molecules have been shown to artificially induce epigenetic modifications in a sequence independent manner. Recently, we showed that specific DNA binding hairpin pyr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00544
更新日期:2012-01-01 00:00:00
abstract::A host of hormonal-metabolic alterations take place following exposure of cardiomyocytes to hypoxia and other noxious stimuli. Here, we demonstrate that exposure of cultured rat cardiomyocytes to lipopolysaccharide (LPS) resulted in upregulation (~1.5 fold) of oxidized low-density lipoprotein receptor-1 (LOX-1). There...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55694-7
更新日期:2019-12-17 00:00:00
abstract::Wood density (WD) relates to important tree functions such as stem mechanics and resistance against pathogens. This functional trait can exhibit high intraindividual variability both radially and vertically. With the rise of LiDAR-based methodologies allowing nondestructive tree volume estimations, failing to account ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-58733-w
更新日期:2020-02-06 00:00:00
abstract::The β5 subunit of the proteasome has been shown in worms and in human cell lines to be regulatory. In these models, β5 overexpression results in upregulation of the entire proteasome complex which is sufficient to increase proteotoxic stress resistance, improve metabolic parameters, and increase longevity. However, fu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39508-4
更新日期:2019-02-28 00:00:00
abstract::Coleoptera (beetles) is a massively successful order of insects, distinguished by their evolutionarily modified forewings called elytra. These structures are often presumed to have been a major driving force for the successful radiation of this taxon, by providing beetles with protection against a variety of harsh env...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34813
更新日期:2016-10-06 00:00:00
abstract::Behavioural studies revealed that the dog-human relationship resembles the human mother-child bond, but the underlying mechanisms remain unclear. Here, we report the results of a multi-method approach combining fMRI (N = 17), eye-tracking (N = 15), and behavioural preference tests (N = 24) to explore the engagement of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79247-5
更新日期:2020-12-17 00:00:00
abstract::High and low hematocrit (Hct) and hemoglobin (Hb) levels are associated with the risk of cardiovascular disease. The purpose of this study was to determine the relationships of Hct, Hb and red blood cells (RBCs) with vascular function and structure. We measured flow-mediated vasodilation (FMD), nitroglycerin-induced v...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68319-1
更新日期:2020-07-10 00:00:00
abstract::Some neural network can be trained by transfer learning, which uses a pre-trained neural network as the source task, for a small target task's dataset. The performance of the transfer learning depends on the knowledge (i.e., layers) selected from the pre-trained network. At present, this knowledge is usually chosen by...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64165-3
更新日期:2020-05-18 00:00:00
abstract::It is known that a developing avian embryo resorbs micronutrients (calcium and other chemical elements) from the inner layer of the eggshell, inducing thinning and overall changes in the shell's chemical composition. However, an aspect yet to be explored relates to the local changes in the multi-elemental composition ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79040-4
更新日期:2020-12-17 00:00:00
abstract::We developed a new technique to observe macroautophagy in the brain in vivo, and examined whether fasting induced macroautophagy in neurons and how the induction was different between Alzheimer's disease (AD) model and control mice. Lentivirus for EGFP-LC3 injected into the brain successfully visualized autophagosome ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12115
更新日期:2015-07-14 00:00:00