Abstract：
OBJECTIVES:The aim of this study was to investigate the methylation status and mRNA expression levels of P15, death-associated protein kinase (DAPK), and suppressor of cytokine signaling-1 (SOCS1) genes in multiple myeloma (MM). MATERIALS AND METHODS:The bone marrow samples of 54 MM patients were collected and the methylation status of the P15, DAPK, and SOCS1 gene promoter regions was determined by methylation-specific polymerase chain reaction. Automated sequencing technology was used to sequence the amplified products in order to analyze the base methylation sites. mRNA expression levels were determined using real-time fluorescent quantitative polymerase chain reaction. RESULTS:Among the 54 MM patients, the positive methylation rates of the P15, DAPK, and SOCS1 genes were 27.78%, 18.52%, and 16.67%, respectively. The methylation results were confirmed by sequencing. The positive methylation rates of the P15, DAPK, and SOCS1 genes showed no correlation with patient gender, age, typing, staging, and grouping (P>0.05). There was no significant difference in the mRNA expression levels of the P15, DAPK, and SOCS1 genes between the MM patient group and the control group (P>0.05). CONCLUSIONS:Aberrant methylation of the P15, DAPK, and SOCS1 genes exists in MM, and these genes may play certain roles in pathogenesis of MM. There was no significant difference in mRNA expression levels between the methylated group and the non-methylated group, suggesting that these genes are regulated by other mechanisms during their transcription.
journal_name
Iran J Basic Med Scijournal_title
Iranian journal of basic medical sciencesauthors
Liu L,Tan L,He Zsubject
Has Abstractpub_date
2016-07-01 00:00:00pages
755-62issue
7eissn
2008-3866issn
2008-3874journal_volume
19pub_type
杂志文章abstract：OBJECTIVES:Various studies have shown that the diabetes is associated with liver failure. The objective of this study was determining the effects of Berberis vulgaris fruit on histopathological and biochemical markers of liver in diabetic rats. MATERIALS AND METHODS:In this experimental study, 60 male Wistar rats weig...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2017.8681
更新日期：2017-05-01 00:00:00
abstract：OBJECTIVES:Hyperthermia is one of the most common environmental stressors that affect multi-biological systems in the body including the central nervous system as well as the hematopoietic organs. The objective of the present study was to investigate the protective role of ethanolic extract of propolis (EEP) on some se...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVES:Parkinson's disease (PD) is a progressive neurological disorder associated with motor disabilities and cognitive dysfunction as well. Evidence indicates that PD occurs less frequently in women than men, confirming a role for steroid hormones in protection of dopaminergic nigrostriatal neurons. It is reported...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2016.7911
更新日期：2016-12-01 00:00:00
abstract：OBJECTIVES:Clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to examine the impression of a combination therapy. This was performed by intravenous injection of human umbilical cord matrix stem cell (hUCMSC-Wharton(,)s jelly stem cell) with bone marrow cell mobilized by gr...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVES:To clarify the protective effects of Cichorium glandulosum (CG) extracts on thioacetamide (TAA)-induced rat hepatic fibrosis. MATERIALS AND METHODS:The dry roots of CG were smashed and percolated with 95% ethanol, and the residual was prepared into petroleum ether extract (CG-V), ethyl acetate extract (CG-V...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-11-01 00:00:00
abstract：Objectives:Periodontal diseases are among prevalent oral health problems which may ultimately lead to severe complications in oral cavity. Herbal products can be designed as single or multicomponent preparations for better oral health. This study aims to review current clinical trials on the effectiveness of herbal pro...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章,评审
doi：10.22038/IJBMS.2018.31997.7690
更新日期：2018-10-01 00:00:00
abstract：OBJECTIVES:Prostate cancer is the world's leading cause of cancer and the second cause of cancer-related death in men after lung cancer. Differentiation of prostate adenocarcinoma from benign prostate lesions and hyperplasia sometimes cannot be done on the basis of morphologic findings. Considering the fact that in the...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVES:This study aimed to evaluate the protective effects of total flavonoid extract from Coreopsis tinctoria Nutt. (CTF) against myocardial ischemia/reperfusion injury (MIRI) using an isolated Langendorff rat heart model. MATERIALS AND METHODS:Left ventricular developed pressure (LVDP) and the maximum rate of ri...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVES:Lectin-like low density lipoprotein receptor (LOX-1) has pivot role in vascular complications, which is upregulated in numerous pathological conditions. Since exercise has beneficial effects in prevention of hyperlipidemic complications, present study examined protective effects of aerobic exercise through r...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVES:Psoriasis is an autoimmune disease that appears on the skin. Although psoriasis is clinically and histologically well characterized, its pathogenesis is unknown in detail. The aims of this study were to evaluate the proteome of psoriatic patients' sera and to compare them with those of normal healthy human t...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：Objectives:Cholinergic neurons are important neurons in the Pedunculopontine tegmental nucleus (PPT). In this study, nicotinic receptor of the PPT in central cardiovascular regulation in the anesthetized rat was evaluated. Materials and Methods:Saline, acetylcholine (Ach; doses: 90 and 150 nmol), hexamethonium (Hexa; ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.25616.6319
更新日期：2018-04-01 00:00:00
abstract：OBJECTIVES:Obesity considered being a low-grade inflammatory disease. The objective of this study was to examine the association between inflammatory markers (IM) including C-reactive protein (hs-CRP), Interleukin-6 (IL-6), and homocystein (Hcy) and obesity-related factors (e.g. BMI, waist, hip) in adult participants o...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-08-01 00:00:00
abstract：Objectives:Osteonecrosis of the jaw, as an exposed necrotic bone in the oral cavity, is one of the adverse effects of bisphosphonates, which have an affinity for bone minerals. This study investigates the cytotoxic effects of alendronate (ALN) as a nitrogen-containing bisphosphonate, on human dental pulp stem cells (hD...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.22877.5816
更新日期：2018-09-01 00:00:00
abstract：Objectives:Precision-cut tissue slices are considered an organotypic 3D model widely used in biomedical research. The comet assay is an important screening test for early genotoxicity risk assessment that is mainly applied on in vitro models. The aim of the present study was to provide a 3D organ system for determinati...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.30056.7242
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVE(S):Carotenoids which are naturally synthesized by fungi such as yeasts can act as an antioxidant which is closely related to their ability to decrease the risk of a variety of degenerative diseases. In recent years, the increase of demand for carotenoids obtained from natural sources has promoted major effort...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：Objectives:Cord blood (CB) is known as a valuable source of hematopoietic stem cells (HSC). Identifying strategies that enhance expansion and maintain engraftment and homing capacity of HSCs can improve transplant efficiency. In this study, we examined different culture conditions on ex vivo expansion and homing capaci...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2018.26820.6561
更新日期：2018-07-01 00:00:00
abstract：OBJECTIVES:Intracerebral injection of bone marrow stromal cells (BMSCs) is being investigated as a therapeutic tool to prevent Alzheimer's disease (AD). Our aim was to investigate the effects of BMSCs by intrathecal injection in AD rat model. MATERIALS AND METHODS:BMSCs were obtained from the bone marrow of Wistar rat...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
abstract：OBJECTIVES:Primary or adult type hypolactasia, the most common enzyme deficiency in the world, is due to reduced lactase activity in the intestinal cell after weaning. Lactase non-persistence is inherited as an autosomal recessive trait. A DNA variant, single nucleotide polymorphism C/T-13910 which is located on 13910 ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVES:Cyclooxygenases (COXs) are known to play some roles in physiological mechanisms related to learning and memory. Since sodium salicylate is an inhibitor of COX, we have evaluated the effect of peripheral and intra-hippocampal administration of sodium salicylate on spatial learning and memory in male rats. MA...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVES:Morphine is widely used to treat chronic pain. However, its utility is hindered by the development of tolerance to its analgesic effects. Despite the renowned beneficial effects of physical exercise on cognitive functions and signs of morphine withdrawal in morphine-dependent rats, little is known about the ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：Objectives:Prenatal stresses increase incidence of neurodevelopmental disorders and influence cognitive abilities. Glucocorticoids are released in stress condition as endpoint activation of hypothalamus-pituitary-adrenal (HPA) axis. Evidence indicates a cross-talk between gut microbiota and brain function. This study a...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.33956.8078
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVES:To optimize the extraction method using response surface methodology, extract the phenolic compounds, and identify the antioxidant and biological properties of Stachys parviflora L. extracts. MATERIALS AND METHODS:Maceration and ultrasound-assisted extraction (UAE) (4, 7, 10 min treatment time, 40, 70, 100 ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVES:This study examined whether conjugated linoleic acid (CLA) supplementation affects insulin sensitivity and peroxisome proliferator-activated receptor gamma (PPAR-γ) and glucose transporter type 4 (GLUT-4) protein expressions in the skeletal muscles of rats during endurance exercise. MATERIALS AND METHODS:Sp...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVES:Recently, we showed that some new synthetic compounds structurally related to cilostamide (4-(1,2-dihydro-2-oxoquinolin-6-hydroxy)- N-cyclohexyl-N-methylbutanamide), a selective phosphodiesterase 3 (PDE3) inhibitor, produce inotropic effect comparable to that of IBMX (3-isobutyl-1-methylxanthine), a non-sele...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2017.8831
更新日期：2017-06-01 00:00:00
abstract：Objectives:The present study aimed to determine and compare moisturizing and occlusion effects of different solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) using magnetized water and deionized water. Materials and Methods:SLN formulations were prepared using various lipids, including Tripalmiti...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/IJBMS.2020.39587.9397
更新日期：2020-03-01 00:00:00
abstract：OBJECTIVES:Asthma results from the interaction between genetic and environmental factors. ADAM33 gene on chromosome 20p13 is associated with asthma and airway hyperresponsiveness. MATERIALS AND METHODS:This is a case-control study, where four SNPs S1 (rs3918396), T1 (rs2280091), T2 (rs2280090), V4 (rs2787094) of ADAM3...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：OBJECTIVE(S):Spermatogonial Stem Cells (SSCs) maintain spermatogenesis throughout the life of the male. Because of the small number of SSCs in adult, enriching and culturing them is a crucial step prior to differentiation or transplantation. Maintenance of SSCs and transplantation or induction of in vitro spermio-genes...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2013-02-01 00:00:00
abstract：OBJECTIVES:The aim of the study was to investigate the relationship between insulin resistance (IR) and leptin (LEP) gene polymorphisms in peritoneal dialysis (PD) patients. MATERIALS AND METHODS:From July 1, 2011 to August 1, 2011, patients who received chronic PD were chosen and divided into three groups (DM, high H...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVE(S):Staphylococcus aureus is both a successful human commensal and a major pathogen. In this study we investigated the genetic diversity of 26 S. aureus isolates recovered from human skin and urinary tract infections. MATERIALS AND METHODS:Typing procedure for the studied S. aureus isolates was performed base...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：
更新日期：2012-07-01 00:00:00
abstract：Objectives:The preclinical reports have shown that specific probiotics like Bifidobacterium bifidum (B. bifidum) and Lactobacillus acidophilus (L. acidophilus) can be applied as the biotherapeutic agents in the inhibition or therapy of colorectal cancer via the modification of gut bacteria. In the previous studies, we ...
journal_title：Iranian journal of basic medical sciences
pub_type： 杂志文章
doi：10.22038/ijbms.2019.32624.7806
更新日期：2019-06-01 00:00:00
