Abstract:
OBJECTIVE(S):Spermatogonial Stem Cells (SSCs) maintain spermatogenesis throughout the life of the male. Because of the small number of SSCs in adult, enriching and culturing them is a crucial step prior to differentiation or transplantation. Maintenance of SSCs and transplantation or induction of in vitro spermio-genesis may provide a therapeutic strategy to treat male infertility. This study investigated the enrichment and proliferation of SSCs co-cultured with STO cells in the presence or absence of growth factors. MATERIALS AND METHODS:Spermatogonial populations were enriched from the testis of 4-6 week-old male mice by MACS according to the expression of a specific marker, Thy-1. Isolated SSCs were cultured in the presence or absence of growth factors (GDNF, GFRα1 and EGF) on STO or gelatin-coated dishes for a week. Subsequently, the authors evaluated the effects of growth factors and STO on SSCs colonization by alkaline phosphates (AP) activity and flow cytometery of α6 and β1 integrins. RESULTS:SSCs co-cultured with STO cells and growth factors developed colonization and AP activity as well as expression of α6 and β1 integrins (P≤0/05). CONCLUSION:Our present SSC-STO co-culture provides conditions that may allow efficient maintenance and proliferation of SSCs for the treatment of male infertility.
journal_name
Iran J Basic Med Scijournal_title
Iranian journal of basic medical sciencesauthors
Rastegar T,Minaee MB,Habibi Roudkenar M,Raghardi Kashani I,Amidi F,Abolhasani F,Barbarestani Msubject
Has Abstractpub_date
2013-02-01 00:00:00pages
134-9issue
2eissn
2008-3866issn
2008-3874journal_volume
16pub_type
杂志文章abstract:OBJECTIVES:Various studies have shown that the diabetes is associated with liver failure. The objective of this study was determining the effects of Berberis vulgaris fruit on histopathological and biochemical markers of liver in diabetic rats. MATERIALS AND METHODS:In this experimental study, 60 male Wistar rats weig...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/IJBMS.2017.8681
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVES:Cyclooxygenases (COXs) are known to play some roles in physiological mechanisms related to learning and memory. Since sodium salicylate is an inhibitor of COX, we have evaluated the effect of peripheral and intra-hippocampal administration of sodium salicylate on spatial learning and memory in male rats. MA...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVES:This study examined whether conjugated linoleic acid (CLA) supplementation affects insulin sensitivity and peroxisome proliferator-activated receptor gamma (PPAR-γ) and glucose transporter type 4 (GLUT-4) protein expressions in the skeletal muscles of rats during endurance exercise. MATERIALS AND METHODS:Sp...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract:Objectives:Cholinergic neurons are important neurons in the Pedunculopontine tegmental nucleus (PPT). In this study, nicotinic receptor of the PPT in central cardiovascular regulation in the anesthetized rat was evaluated. Materials and Methods:Saline, acetylcholine (Ach; doses: 90 and 150 nmol), hexamethonium (Hexa; ...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/IJBMS.2018.25616.6319
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVE(S):Gingivitis is an inflammatory disease that affects tooth-supporting tissues and is caused by a microbe-immune response. The aim of this study was to evaluate the effects of omega-3 fatty acids on immune system regulation and the prevention and treatment of gingivitis using an animal model. MATERIALS AND M...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2014-02-01 00:00:00
abstract::Sulfur mustard (SM, bis- (2-chloroethyl) sulphide) is a chemical warfare agent that causes DNA alkylation, protein modification and membrane damage. SM can trigger several molecular pathways involved in inflammation and oxidative stress, which cause cell necrosis and apoptosis, and loss of cells integrity and function...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVES:Human Cytomegalovirus (HCMV) remains a major morbidity and mortality cause in immuno suppressed patients. Therefore, significant effort has been made towards the development of a vaccine. In this study, the expression of the pp65 and gB fusion peptides and Fc domain of mouse IgG2a as a novel delivery system ...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVES:To study the effect of low-frequency vibration on bone marrow stromal cell differentiation and potential bone repair in vivo. MATERIALS AND METHODS:Forty New Zealand rabbits were randomly divided into five groups with eight rabbits in each group. For each group, bone defects were generated in the left humer...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/ijbms.2017.8088
更新日期:2017-01-01 00:00:00
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journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2016-10-01 00:00:00
abstract:OBJECTIVES:Fumonisins are a group of toxic and carcinogenic mycotoxins, which contaminate the grains and their products. The aim of this study was to examine the apoptotic and proliferative activity of mouse gastric mucosa following administration of fumonisin B1 (FB1). MATERIALS AND METHODS:Twenty-nine female mice di...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVES:Lipocalin 2 (Lcn2) is a 25-kDa glycoprotein that has initially been extracted from neutrophil granules. Expression of Lcn2 is induced under various pathophysiological conditions. It is also known as an early marker of kidney and heart injury. High-level expression of recombinant Lcn2 neutrophil gelatinase-as...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVES:Crocin bleaching assay (CBA) is a new method for determination of antioxidant capacity. In CBA, addition of hydrogen to the conjugated double bonds of crocin results in reduction of crocin and increase in the absorbance at 440 nm, which is considered as a measure of antioxidant potential. Here CBA method was...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2011-09-01 00:00:00
abstract:OBJECTIVES:Valproic acid (VPA) has proven to be as one of the most promising useful drug with anticancer properties. In this study, we investigate the VPA effects on E-cadherin expression in HeLa, TC1, MKN45, and HCT116 cell lines. This study assesses the effects of VPA on human papillomavirus E7 expression in HPV posi...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE(S):In the current study, the effects of selected folk medicinal herbs were evaluated in D-galactose-induced aging in male mice. MATERIALS AND METHODS:Male BALB/c mice were randomly divided into 12 groups composing sham, control, and treated groups. Aging was induced by administration of D-galactose (500 mg/k...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was to prepare, characterize, and evaluate solid lipid nanoparticles (SLNs) containing Zataria multiflora essential oil (ZEO). MATERIALS AND METHODS:In this study, Z. multiflora essential oil-loaded solid lipid nanoparticles (ZE-SLNs) were prepared to improve its efficiency in c...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVES:Increasing evidence in both experimental and clinical studies suggests that oxidative stress plays a major role in the pathogenesis of multiple sclerosis. The aim of the present work is to investigate the protective effects of erythropoietin against cuprizone-induced oxidative stress. MATERIALS AND METHODS:...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/IJBMS.2017.9110
更新日期:2017-08-01 00:00:00
abstract:Objectives:Neurodegeneration is an outcome of Methamphetamine (METH) abuse. Studies have emphasized on the neuroprotective properties of lithium. The current study is designed towards evaluating the role of Akt-1/GSK3 and CREB-BDNF signaling pathways in mediating lithium neuroprotection against METH-induced neurodegene...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/ijbms.2019.30855.7442
更新日期:2019-08-01 00:00:00
abstract::Klotho (KL) encodes a single-pass transmembrane protein and is predominantly expressed in the kidney, parathyroid glands, and choroid plexus. Genetic studies on the KL gene have revealed that DNA hypermethylation is one of the major risk factors for aging, diseases, and cancer. Besides, KL exerts anti-inflammatory and...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVES:Prostaglandins have been shown to mediate the gastro-protective effect of sodium hydrosulfide (NaHS) but effect of NaHS on mRNA expression of prostaglandin E2 receptors (EP1, 3-4; EPs) has not been investigated. Therefore, this study designed to evaluate the effect of NaHS on mRNA expression of EPs receptors...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/ijbms.2017.8237
更新日期:2017-02-01 00:00:00
abstract:Objectives:In addition to genetic factors, environmental phenomena during postnatal age highly affect development and, in turn, function of the brain. The present work evaluates if morphine consumption during lactation period influences the spatial performances and synaptic plasticity in rats at neonatal period of age....
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/ijbms.2019.36892.8789
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:Amyotrophic lateral sclerosis (ALS), a fatal progressive neurodegenerative disorder, is the most common motor neuron disease in European populations. Approximately 10% of ALS cases are familial (FALS) and the other patients are considered as sporadic ALS (SALS). Among many ALS causing genes that have been id...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2014-10-01 00:00:00
abstract:Objectives:The aim of the present research is to investigate the efficacy of L-carnitine (LC) as a complementary therapy to diclofenac sodium (Dic) treatment in a mono-iodoacetate (MIA) induced knee osteoarthritis (OA) rat model, with respect to pain relief and the underlying pathology. Materials and Methods:Fifty adu...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/ijbms.2020.43136.10138
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVE(S):Green tea (GT) is one of the most popular beverages worldwide whose beneficial effects on health have been demonstrated. Recent studies suggest that GT may contribute to reduction of cancer risk and progression. The aim of this study was to evaluate the effects of whole GT on urinary bladder chemical carci...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2014-02-01 00:00:00
abstract:Objectives:Heparin-binding hemagglutinin (HBHA), a mycobacterial cell surface protein, mediates adhesion to nonphagocytic cells and the dissemination of Mycobacterium tuberculosis (M. tuberculosis) from the site of primary infection. Superior expression systems are required to obtain abundant M. tuberculosis proteins f...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/IJBMS.2018.24280.6064
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:In previous studies, researchers observed that doxepin could improve cognitive processes and has protective effects on the central nervous system. Thus, this study was designed to analyze the effects of doxepin on β-amyloid (Aβ)-induced memory impairment and neuronal toxicity in rat and to explore the underl...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/IJBMS.2017.9274
更新日期:2017-09-01 00:00:00
abstract::The study of tumor viruses paves the way for understanding the mechanisms of virus pathogenesis, including those involved in establishing infection and dissemination in the host tumor affecting immune-compromised patients. The processes ranging from viral infection to progressing malignancy are slow and usually insuff...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章,评审
doi:
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVES:Herbal waters are extensively used in most parts of including . Visiting a patient with total blindness due to daily ingestion of around 200 ml of herbal water (Plant forty water) per day for six months was the rational for methanol determination in all herbal waters available in markets. MATERIALS AND METH...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVES:Neuroprotective effect of creatine (Cr) against β-amyloid (Aβ) is reported in an in vitro study. This study investigated the effect of Cr supplementation on β-amyloid toxicity in vivo. MATERIALS AND METHODS:Thirty two, male Wistar rats were divided into 4 groups. During ten weeks of study, control group wen...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2016-11-01 00:00:00
abstract:Objectives:Gallic acid (GA) is a highly effective antioxidant, which its beneficial effects are well known, but its impact on expression of microRNAs (miRs) following hepatic ischemia-reperfusion (I/R) is not well recognized. Therefore, the current research was designed to specify the beneficial effect of GA on miRs (1...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:10.22038/ijbms.2018.31589.7605
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVES:Production of extended-spectrum beta-lactamases (ESBLs) by enteric bacteria continues to be a major problem in hospitals and community. ESBLs producing bacteria cause many serious infections including urinary tract infections, peritonitis, cholangitis and intra-abdominal abscess. The aim of this study was to...
journal_title:Iranian journal of basic medical sciences
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00