Abstract：
:Lon is an essential, multitasking AAA(+) protease regulating many cellular processes in species across all kingdoms of life. Altered expression levels of the human mitochondrial Lon protease (hLon) are linked to serious diseases including myopathies, paraplegia, and cancer. Here, we present the first 3D structure of full-length hLon using cryo-electron microscopy. hLon has a unique three-dimensional structure, in which the proteolytic and ATP-binding domains (AP-domain) form a hexameric chamber, while the N-terminal domain is arranged as a trimer of dimers. These two domains are linked by a narrow trimeric channel composed likely of coiled-coil helices. In the presence of AMP-PNP, the AP-domain has a closed-ring conformation and its N-terminal entry gate appears closed, but in ADP binding, it switches to a lock-washer conformation and its N-terminal gate opens, which is accompanied by a rearrangement of the N-terminal domain. We have also found that both the enzymatic activities and the 3D structure of a hLon mutant lacking the first 156 amino acids are severely disturbed, showing that hLon's N-terminal domains are crucial for the overall structure of the hLon, maintaining a conformation allowing its proper functioning.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kereïche S,Kováčik L,Bednár J,Pevala V,Kunová N,Ondrovičová G,Bauer J,Ambro Ľ,Bellová J,Kutejová E,Raška Idoi
10.1038/srep33631subject
Has Abstractpub_date
2016-09-16 00:00:00pages
33631issn
2045-2322pii
srep33631journal_volume
6pub_type
杂志文章abstract：:Pertussis is a highly contagious disease for which prompt, point-of-care diagnosis remains an unmet clinical need. Results from conventional test modalities (nucleic acid detection, serology, and culture) take hours to days. To overcome this challenge, we identified a new biomarker (tracheal colonization factor A, Tcf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72092-6
更新日期：2020-09-14 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-35437-w
更新日期：2018-11-22 00:00:00
abstract：:Visceral leishmaniasis (VL) is a fatal form of leishmaniasis which affects 70 countries, worldwide. Increasing drug resistance, HIV co-infection, and poor health system require operative vaccination strategy to control the VL transmission dynamics. Therefore, a holistic approach is needed to generate T and B memory ce...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08842-w
更新日期：2017-08-15 00:00:00
abstract：:Prostate cancer (PCa) is the most common solid neoplasm diagnosed in developed countries. Nerve-sparing radical prostatectomy (NS-RP) has been widely accepted as the best choice treatment for localised PCa. However, erectile dysfunction (ED) and urinary incontinence are commonly observed after NS-RP. Using meta-analys...
journal_title：Scientific reports
pub_type： 杂志文章,评审
doi：10.1038/srep05801
更新日期：2014-07-23 00:00:00
abstract：:ATM is an important cancer susceptibility gene that encodes a critical apical kinase of the DNA damage response (DDR) pathway. We show that a key nonsense-mediated RNA decay switch exon (NSE) in ATM is repressed by U2AF, PUF60 and hnRNPA1. The NSE activation was haplotype-specific and was most promoted by cytosine at ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18741
更新日期：2016-01-06 00:00:00
abstract：:The intricate molecular processes underlying photosynthesis have long been studied using various analytic approaches. However, the three-dimensional (3D) dynamics of such photosynthetic processes remain unexplored due to technological limitations related to investigating intraorganellar mechanisms in vivo. By developi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29940
更新日期：2016-07-15 00:00:00
abstract：:Electrospinning can be used to mimic the architecture of an acellular nerve graft, combining microfibers for guidance, and pores for cellular infiltration. We made electrospun nerve guides, from polycaprolactone (PCL) or poly-L-lactic acid (PLLA), with aligned fibers along the insides of the channels and random fibers...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34699-8
更新日期：2018-11-13 00:00:00
abstract：:Identification of patients with early stage non-small cell lung cancer (NSCLC) with high risk of recurrence could help identify patients who would receive additional benefit from adjuvant therapy. In this work, we present a computational histomorphometric image classifier using nuclear orientation, texture, shape, and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13773-7
更新日期：2017-10-19 00:00:00
abstract：:Climate change profoundly influences the geospatial distribution of secondary metabolites and causes the geographical migration of plants. We planted seedlings of the same species in eighteen ecological regions along a latitudinal gradient in eastern and western China, in order to explore the regulation of multi-clima...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36729-x
更新日期：2019-01-29 00:00:00
abstract：:The Kidney Donor Profile Index (KDPI) was introduced in the United States in 2014 to guide the decision making of clinicians with respect to accepting or declining a donated kidney. To evaluate whether the KDPI can be applied to a European cohort, we retrospectively assessed 580 adult patients who underwent renal tran...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47772-7
更新日期：2019-08-02 00:00:00
abstract：:We present results from first-principles calculations on silane (SiH4) under pressure. We find that a three dimensional P-3 structure becomes the most stable phase above 241 GPa. A prominent structural feature, which separates the P-3 structure from previously observed/predicted SiH4 structures, is that a fraction of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13039
更新日期：2015-08-12 00:00:00
abstract：:Seeded polymerisation of proteins forming amyloid fibres and their spread in tissues has been implicated in the pathogenesis of multiple neurodegenerative diseases: so called "prion-like" mechanisms. While ex vivo mammalian prions, composed of multichain assemblies of misfolded host-encoded prion protein (PrP), act as...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36700-w
更新日期：2019-01-23 00:00:00
abstract：:Detection of subclinical mastitis (SCM) in its initial stage can save great economic losses, improve milk quality and animal welfare. We have developed a semiquantitative lateral flow assay for the detection of SCM in dairy cows targeting myeloperoxidase (MPO) enzyme of milk neutrophils. A competitive immunoassay form...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68174-0
更新日期：2020-07-07 00:00:00
abstract：:Realization of phosphor-free white-light emitters is becoming an important milestone on the road to achieve high quality and reliability in high-power white-light-emitting diodes (LEDs). However, most of reported methods have not been applied to practical use because of their difficulties and complexity. In this study...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17372
更新日期：2015-12-02 00:00:00
abstract：:As nanotechnology becomes increasingly used in biomedicine, it is important to have techniques by which to examine the structure and dynamics of biologically-relevant molecules on the surface of engineered nanoparticles. Previous work has shown that Saturation-Transfer Difference (STD)-NMR can be used to explore the i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69185-7
更新日期：2020-07-23 00:00:00
abstract：:No validated screening method currently exists for Chronic Obstructive Pulmonary Disease (COPD) in smokers. Therefore, we constructed a predictive model with simple parameters that can be applied for COPD screening to detect fixed airflow limitation. This observational cross-sectional study included a random sample of...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-018-31198-8
更新日期：2018-09-06 00:00:00
abstract：:We studied the spin-transfer torque (STT) in graphene based normal-metal/topological-insulator/ferromagnet heterostructures (N/TI/F), which is induced by the helical spin-polarized current in the quantum spin Hall insulator. We found that the STT is comparable in magnitude to the STT in ferromagnetic-normal- ferromagn...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22680-4
更新日期：2018-03-12 00:00:00
abstract：:The striatum plays important motor, associative and cognitive roles in brain functions. However, the rodent dorsolateral (the primate putamen) and dorsomedial (the primate caudate nucleus) striatum are not anatomically separated, making it difficult to distinguish their functions. By contrast, anatomical separation ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35809
更新日期：2016-11-02 00:00:00
abstract：:The Alzheimer's disease pathology is associated with accumulation of intracellular neurofibrillary tangles and extracellular senile plaques. The formation of initial nucleus triggers conformational changes in Tau and leads to its deposition. Hence, there is a need to eliminate these toxic proteins for proper functioni...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60773-1
更新日期：2020-03-04 00:00:00
abstract：:Matrices for sustained release of drugs have been based on polymers, biomaterials and oxides. The use of the major family of metals as matrices for sustained release is, to the best of our knowledge, unknown. In this context we describe a new family of bio-composites for sustained release of drugs, namely analgesic dr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03195-w
更新日期：2017-06-23 00:00:00
abstract：:Prior ex vivo histological postmortem studies of autism spectrum disorder (ASD) have shown gray matter microstructural abnormalities, however, in vivo examination of gray matter microstructure in ASD has remained scarce due to the relative lack of non-invasive methods to assess it. The aim of this work was to evaluate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78486-w
更新日期：2020-12-08 00:00:00
abstract：:Inhibitors of cyclin-dependent kinases (ICKs) are key regulators of cyclin-dependent kinase activities and cell division. Herein, we identified eight ICKs in maize, which we named Zeama;ICKs (ZmICKs). Primary sequencing and phylogenetic analyses were used to divide the ZmICK family into two classes: group B and group ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43818
更新日期：2017-03-06 00:00:00
abstract：:Synthetic biological circuits that can generate outputs with distinct expression dynamics are useful for a variety of biomedical and industrial applications. We present a method to control output dynamics by altering output mRNA decay rates. Using oscillatory expression of the transcription factor p53 as the circuit r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42509-y
更新日期：2019-04-12 00:00:00
abstract：:A new concept for treatment of infections is induction of our own antimicrobial peptides and the presented novel class of inducer, aroylated phenylenediamines (APDs), gives up to 20 to 30-fold induction of the human antimicrobial peptide LL-37, in vitro. In addition, oral administration of an APD in a rabbit model of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36692
更新日期：2016-11-09 00:00:00
abstract：:A generalization of the Raman scattering (RS) spectrum, the Raman excitation map (REM) is a hyperspectral two-dimensional (2D) data set encoding vibrational spectra, electronic spectra and their coupling. Despite the great potential of REM for optical sensing and characterization with remarkable sensitivity and select...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65757-9
更新日期：2020-06-08 00:00:00
abstract：:RNA-based three-way junctions (3WJs) are naturally occurring structures found in many functional RNA molecules including rRNA, tRNA, snRNA and ribozymes. 3WJs are typically characterized as resulting from an RNA molecule folding back on itself in cis but could also form in trans when one RNA, for instance a microRNA b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45393
更新日期：2017-03-28 00:00:00
abstract：:Anoectochilus roxburghii and Anoectochilus formasanus are the major species of genus Anoectochilus used in traditional Chinese medicine for their abundant content of flavonoids and some other medicinal constituents. In recent years, their wild resources are gradually exhausted due to over-collection and ecological det...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56821-0
更新日期：2019-12-27 00:00:00
abstract：:The efficacy and safety of misoprostol alone for missed abortion varied with different regimens. To evaluate existing evidence for the medical management of missed abortion using misoprostol, we undertook a comprehensive review and meta-analysis. The electronic literature search was conducted using PubMed, the Cochran...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-017-01892-0
更新日期：2017-05-10 00:00:00
abstract：:Behavioural ecology research increasingly focuses on why genetic behavioural variation can persist despite selection. Evolutionary theory predicts that directional selection leads to evolutionary change while depleting standing genetic variation. Nevertheless, evolutionary stasis may occur for traits involved in socia...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08258-6
更新日期：2017-08-31 00:00:00
abstract：:Intracerebroventricular (ICV) administration of ouabain, an inhibitor of the Na, K-ATPase, is an approach used to study the physiological functions of the Na, K-ATPase and cardiotonic steroids in the central nervous system, known to cause mania-like hyperactivity in rats. We describe a mouse model of ouabain-induced m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52058-z
更新日期：2019-10-30 00:00:00
