Probing driving forces for binding between nanoparticles and amino acids by saturation-transfer difference NMR.

Abstract:

:As nanotechnology becomes increasingly used in biomedicine, it is important to have techniques by which to examine the structure and dynamics of biologically-relevant molecules on the surface of engineered nanoparticles. Previous work has shown that Saturation-Transfer Difference (STD)-NMR can be used to explore the interaction between small molecules, including amino acids, and the surface of polystyrene nanoparticles. Here we use STD-NMR to further explore the different driving forces that are responsible for these interactions. Electrostatic effects are probed by using zwitterionic polystyrene beads and performing STD-NMR experiments at high, low, and neutral pH, as well as by varying the salt concentration and observing the effect on the STD buildup curve. The influence of dispersion interactions on ligand-nanoparticle binding is also explored, by establishing a structure-activity relationship for binding using a series of unnatural amino acids with different lengths of hydrophobic side chains. These results will be useful for predicting which residues in a peptide are responsible for binding and for understanding the driving forces for binding between peptides and nanoparticles in future studies.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Xu H,Casabianca LB

doi

10.1038/s41598-020-69185-7

subject

Has Abstract

pub_date

2020-07-23 00:00:00

pages

12351

issue

1

issn

2045-2322

pii

10.1038/s41598-020-69185-7

journal_volume

10

pub_type

杂志文章
  • Three-dimensional Fe2O3 nanocubes/nitrogen-doped graphene aerogels: nucleation mechanism and lithium storage properties.

    abstract::We developed a solvothermal-induced self-assembly approach to construct three dimensional (3D) macroscopic Fe2O3 nanocubes/nitrogen-doped graphene (Fe2O3-NC/GN) aerogel as anode materials for lithium-ion batteries (LIBs). The Fe2O3 nanocubes with length of ~50 nm are homogeneously anchored on 3D GN frameworks and as s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep07171

    authors: Wang R,Xu C,Sun J,Gao L

    更新日期:2014-11-25 00:00:00

  • Fast-response, sensitivitive and low-powered chemosensors by fusing nanostructured porous thin film and IDEs-microheater chip.

    abstract::The chemiresistive thin film gas sensors with fast response, high sensitivity, low power consumption and mass-produced potency, have been expected for practical application. It requires both sensitive materials, especially exquisite nanomaterials, and efficient substrate chip for heating and electrical addressing. How...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01669

    authors: Dai Z,Xu L,Duan G,Li T,Zhang H,Li Y,Wang Y,Wang Y,Cai W

    更新日期:2013-01-01 00:00:00

  • miR-29b regulates expression of collagens I and III in chondrogenically differentiating BMSC in an osteoarthritic environment.

    abstract::Osteoarthritis (OA) is characterized by a slowly progressing, irreversible loss of articular cartilage. Tissue engineering approaches for cartilage regeneration include stem cell-based strategies but not much is known about their repair capacity in an OA microenvironment. The aim of the present study was to identify f...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-13567-x

    authors: Mayer U,Benditz A,Grässel S

    更新日期:2017-10-16 00:00:00

  • ETS Related Gene mediated Androgen Receptor Aggregation and Endoplasmic Reticulum Stress in Prostate Cancer Development.

    abstract::Mechanistic studies of deregulated ERG in prostate cancer and other cancers continue to enhance its role in cancer biology and its utility as a biomarker and therapeutic target. Here, we show that ERG, through its physical interaction with androgen receptor, induces AR aggregation and endoplasmic reticulum stress in t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01187-4

    authors: Sreenath TL,Macalindong SS,Mikhalkevich N,Sharad S,Mohamed A,Young D,Borbiev T,Xavier C,Gupta R,Jamal M,Babcock K,Tan SH,Nevalainen MT,Dobi A,Petrovics G,Sesterhenn IA,Rosner IL,Bieberich CJ,Nelson P,Vasioukhin V,

    更新日期:2017-04-24 00:00:00

  • Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid β-oxidation in myocardial injury protection.

    abstract::Metabonomics/metabolomics is a rapid technology for comprehensive profiling of small molecule metabolites in cells, tissues, or whole organisms, the application of which has led to understanding pathophysiologic mechanisms of cardiometabolic diseases, defining predictive biomarkers for those diseases, and also assessi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09547-w

    authors: Jiang M,Wang Q,Chen J,Wang Y,Fan G,Zhu Y

    更新日期:2017-08-18 00:00:00

  • Rottlerin-mediated inhibition of Toxoplasma gondii growth in BeWo trophoblast-like cells.

    abstract::Autophagy is a crucial and physiological process for cell survival from yeast to mammals, including protozoan parasites. Toxoplasma gondii, an intracellular parasite, typically exploits autophagic machinery of host cell; however host cell upregulates autophagy to combat the infection. Herein we tested the efficacy of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01525-6

    authors: Ietta F,Maioli E,Daveri E,Gonzaga Oliveira J,da Silva RJ,Romagnoli R,Cresti L,Maria Avanzati A,Paulesu L,Barbosa BF,Gomes AO,Roberto Mineo J,Ferro EAV

    更新日期:2017-04-28 00:00:00

  • Aseismic transient during the 2010-2014 seismic swarm: evidence for longer recurrence of M ≥ 6.5 earthquakes in the Pollino gap (Southern Italy)?

    abstract::In actively deforming regions, crustal deformation is accommodated by earthquakes and through a variety of transient aseismic phenomena. Here, we study the 2010-2014 Pollino (Southern Italy) swarm sequence (main shock M W 5.1) located within the Pollino seismic gap, by analysing the surface deformation derived from Gl...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-00649-z

    authors: Cheloni D,D'Agostino N,Selvaggi G,Avallone A,Fornaro G,Giuliani R,Reale D,Sansosti E,Tizzani P

    更新日期:2017-04-12 00:00:00

  • A Single Dose of Modified Vaccinia Ankara expressing Ebola Virus Like Particles Protects Nonhuman Primates from Lethal Ebola Virus Challenge.

    abstract::Ebola virus (EBOV), isolate Makona, was the causative agent of the West African epidemic devastating predominantly Guinea, Liberia and Sierra Leone from 2013-2016. While several experimental vaccine and treatment approaches have been accelerated through human clinical trials, there is still no approved countermeasure ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-19041-y

    authors: Domi A,Feldmann F,Basu R,McCurley N,Shifflett K,Emanuel J,Hellerstein MS,Guirakhoo F,Orlandi C,Flinko R,Lewis GK,Hanley PW,Feldmann H,Robinson HL,Marzi A

    更新日期:2018-01-16 00:00:00

  • Impact of HCV core gene quasispecies on hepatocellular carcinoma risk among HALT-C trial patients.

    abstract::Mutations at positions 70 and/or 91 in the core protein of genotype-1b, hepatitis C virus (HCV) are associated with hepatocellular carcinoma (HCC) risk in Asian patients. To evaluate this in a US population, the relationship between the percentage of 70 and/or 91 mutant HCV quasispecies in baseline serum samples of ch...

    journal_title:Scientific reports

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1038/srep27025

    authors: El-Shamy A,Pendleton M,Eng FJ,Doyle EH,Bashir A,Branch AD

    更新日期:2016-06-01 00:00:00

  • Self-Replication of Localized Vegetation Patches in Scarce Environments.

    abstract::Desertification due to climate change and increasing drought periods is a worldwide problem for both ecology and economy. Our ability to understand how vegetation manages to survive and propagate through arid and semiarid ecosystems may be useful in the development of future strategies to prevent desertification, pres...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep33703

    authors: Bordeu I,Clerc MG,Couteron P,Lefever R,Tlidi M

    更新日期:2016-09-21 00:00:00

  • Effect of multiple cyclic RGD peptides on tumor accumulation and intratumoral distribution of IRDye 700DX-conjugated polymers.

    abstract::Strategic delivery of IRDye 700DX (photosensitizer) is a key for improving its effect in photodynamic therapy. In this study, we have synthesized IRDye 700DX-conjugated polymers containing multiple cyclic RGD peptides to deliver IRDye 700DX selectively to tumor cells and tumor-associated blood vessels overexpressing α...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26593-0

    authors: Dou X,Nomoto T,Takemoto H,Matsui M,Tomoda K,Nishiyama N

    更新日期:2018-05-25 00:00:00

  • Directed differentiation of human induced pluripotent stem cells into mature stratified bladder urothelium.

    abstract::For augmentation or reconstruction of urinary bladder after cystectomy, bladder urothelium derived from human induced pluripotent stem cells (hiPSCs) has recently received focus. However, previous studies have only shown the emergence of cells expressing some urothelial markers among derivatives of hiPSCs, and no repo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46848-8

    authors: Suzuki K,Koyanagi-Aoi M,Uehara K,Hinata N,Fujisawa M,Aoi T

    更新日期:2019-07-19 00:00:00

  • A RANKL mutant used as an inter-species vaccine for efficient immunotherapy of osteoporosis.

    abstract::Anti-cytokine therapeutic antibodies have been demonstrated to be effective in the treatment of several auto-immune disorders. However, The problems in antibody manufacture and the immunogenicity caused by multiple doses of antibodies inspire people to use auto-cytokine as immunogen to induce anti-cytokine antibodies....

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep14150

    authors: Liu C,Zhao Y,He W,Wang W,Chen Y,Zhang S,Ma Y,Gohda J,Ishida T,Walter TS,Owens RJ,Stuart DI,Ren J,Gao B

    更新日期:2015-09-28 00:00:00

  • Alterations of cerebral perfusion in asymptomatic internal carotid artery steno-occlusive disease.

    abstract::Patients with asymptomatic occlusion in the internal carotid arteries (ICA) have been shown to have a better preserved hemodynamic status of the brain as compared to patients with symptoms. This study was aimed to explore the cerebral perfusion alterations in asymptomatic patients using multi-parametric arterial spin-...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02094-4

    authors: Chen YF,Tang SC,Wu WC,Kao HL,Kuo YS,Yang SC

    更新日期:2017-05-12 00:00:00

  • Zika Virus Associated Pathology and Antigen Presence in the Testicle in the Absence of Sexual Transmission During Subacute to Chronic Infection in a Mouse Model.

    abstract::Zika virus (ZIKV) is an arboviral infection that has been shown to be sexually transmitted. The study outlined herein aims to determine if accessory sex glands and epididymal epithelial cells are sources of viral persistence in subacute and chronic ZIKV infection, and if infection of these organs is important in sexua...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44582-9

    authors: Clancy CS,Van Wettere AJ,Morrey JD,Julander JG

    更新日期:2019-06-06 00:00:00

  • 'Candidatus Cochliophilus cryoturris' (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus.

    abstract::Free-living amoebae are well known for their role in controlling microbial community composition through grazing, but some groups, namely Acanthamoeba species, also frequently serve as hosts for bacterial symbionts. Here we report the first identification of a bacterial symbiont in the testate amoeba Cochliopodium. Th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03642-8

    authors: Tsao HF,Scheikl U,Volland JM,Köhsler M,Bright M,Walochnik J,Horn M

    更新日期:2017-06-13 00:00:00

  • Pathogen infection drives patterns of nutrient resorption in citrus plants.

    abstract::Nutrient resorption processes in the plants infected by pathogen remain poorly understood. Huanglongbing (HLB) is a destructive disease of citrus. HLB-pathogen 'Candidatus Liberibacter asiaticus' grows specifically in the phloem of hosts and may cause problems in the plant vascular system after infection. Therefore, i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep14675

    authors: Cao J,Cheng C,Yang J,Wang Q

    更新日期:2015-09-30 00:00:00

  • Functional screening of mammalian mechanosensitive genes using Drosophila RNAi library- Smarcd3/Bap60 is a mechanosensitive pro-inflammatory gene.

    abstract::Disturbed blood flow (d-flow) induces atherosclerosis by altering the expression of mechanosensitive genes in the arterial endothelium. Previously, we identified >580 mechanosensitive genes in the mouse arterial endothelium, but their role in endothelial inflammation is incompletely understood. From this set, we obtai...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36461

    authors: Kumar S,Jang IH,Kim CW,Kang DW,Lee WJ,Jo H

    更新日期:2016-11-07 00:00:00

  • Period doubling induced by thermal noise amplification in genetic circuits.

    abstract::Rhythms of life are dictated by oscillations, which take place in a wide rage of biological scales. In bacteria, for example, oscillations have been proven to control many fundamental processes, ranging from gene expression to cell divisions. In genetic circuits, oscillations originate from elemental block such as aut...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep07088

    authors: Ruocco G,Fratalocchi A

    更新日期:2014-11-18 00:00:00

  • Quantitative x-ray phase imaging at the nanoscale by multilayer Laue lenses.

    abstract::For scanning x-ray microscopy, many attempts have been made to image the phase contrast based on a concept of the beam being deflected by a specimen, the so-called differential phase contrast imaging (DPC). Despite the successful demonstration in a number of representative cases at moderate spatial resolutions, these ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01307

    authors: Yan H,Chu YS,Maser J,Nazaretski E,Kim J,Kang HC,Lombardo JJ,Chiu WK

    更新日期:2013-01-01 00:00:00

  • Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor.

    abstract::Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40305

    authors: Ha NR,Jung IP,La IJ,Jung HS,Yoon MY

    更新日期:2017-01-05 00:00:00

  • Akt regulates neurite growth by phosphorylation-dependent inhibition of radixin proteasomal degradation.

    abstract::Neurite growth is controlled by a complex molecular signaling network that regulates filamentous actin (F-actin) dynamics at the growth cone. The evolutionarily conserved ezrin, radixin, and moesin family of proteins tether F-actin to the cell membrane when phosphorylated at a conserved threonine residue and modulate ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-20755-w

    authors: Jin EJ,Ko HR,Hwang I,Kim BS,Choi JY,Park KW,Cho SW,Ahn JY

    更新日期:2018-02-07 00:00:00

  • Annexin A4-nuclear factor-κB feedback circuit regulates cell malignant behavior and tumor growth in gallbladder cancer.

    abstract::Gallbladder cancer (GBC) is the most common malignant tumor of the biliary system. However, the mechanisms underlying its tumor initiation, progression, and metastasis are not yet fully understood. The annexin A4 (ANXA4) gene is highly expressed in GBC tissues and may play an important role in the initiation and progr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep31056

    authors: Yao HS,Sun C,Li XX,Wang Y,Jin KZ,Zhang XP,Hu ZQ

    更新日期:2016-08-05 00:00:00

  • Structural basis of cell wall peptidoglycan amidation by the GatD/MurT complex of Staphylococcus aureus.

    abstract::The peptidoglycan of Staphylococcus aureus is highly amidated. Amidation of α-D-isoglutamic acid in position 2 of the stem peptide plays a decisive role in the polymerization of cell wall building blocks. S. aureus mutants with a reduced degree of amidation are less viable and show increased susceptibility to methicil...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-31098-x

    authors: Nöldeke ER,Muckenfuss LM,Niemann V,Müller A,Störk E,Zocher G,Schneider T,Stehle T

    更新日期:2018-08-28 00:00:00

  • Rigidity of silicone substrates controls cell spreading and stem cell differentiation.

    abstract::The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy. In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone gels we show t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep33411

    authors: Vertelov G,Gutierrez E,Lee SA,Ronan E,Groisman A,Tkachenko E

    更新日期:2016-09-21 00:00:00

  • Triggering of eruptions at Axial Seamount, Juan de Fuca Ridge.

    abstract::The submarine volcano Axial Seamount has exhibited an inflation predictable eruption cycle, which allowed for the successful forecast of its 2015 eruption. However, the exact triggering mechanism of its eruptions remains ambiguous. The inflation predictable eruption pattern suggests a magma reservoir pressure threshol...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-67043-0

    authors: Cabaniss HE,Gregg PM,Nooner SL,Chadwick WW Jr

    更新日期:2020-06-23 00:00:00

  • The transcriptional response to oxidative stress is part of, but not sufficient for, insulin resistance in adipocytes.

    abstract::Insulin resistance is a major risk factor for metabolic diseases such as Type 2 diabetes. Although the underlying mechanisms of insulin resistance remain elusive, oxidative stress is a unifying driver by which numerous extrinsic signals and cellular stresses trigger insulin resistance. Consequently, we sought to under...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-20104-x

    authors: Chaudhuri R,Krycer JR,Fazakerley DJ,Fisher-Wellman KH,Su Z,Hoehn KL,Yang JYH,Kuncic Z,Vafaee F,James DE

    更新日期:2018-01-29 00:00:00

  • Associations of parental age with health and social factors in adult offspring. Methodological pitfalls and possibilities.

    abstract::Parental age is increasing rapidly in many countries. Analysis of this potentially important influence on offspring well-being is hampered by strong secular trends and socioeconomic patterning and by a shortage of follow-up data for adult offspring. We used Swedish national data on up to 3,653,938 offspring to conside...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep45278

    authors: Carslake D,Tynelius P,van den Berg G,Davey Smith G,Rasmussen F

    更新日期:2017-03-27 00:00:00

  • Enriched environment alleviates stress-induced dry-eye through the BDNF axis.

    abstract::The number of patients with dry eye disease (DED) is increasing, and DED has become an urgent public health problem. A comorbidity of mental disorders has been reported in DED patients. We hypothesized that physical and psychological stressors impair tear secretion. To examine the relationship between stress loading a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39467-w

    authors: Sano K,Kawashima M,Imada T,Suzuki T,Nakamura S,Mimura M,Tanaka KF,Tsubota K

    更新日期:2019-03-04 00:00:00

  • Nanodiamond landmarks for subcellular multimodal optical and electron imaging.

    abstract::There is a growing need for biolabels that can be used in both optical and electron microscopies, are non-cytotoxic, and do not photobleach. Such biolabels could enable targeted nanoscale imaging of sub-cellular structures, and help to establish correlations between conjugation-delivered biomolecules and function. Her...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02668

    authors: Zurbuchen MA,Lake MP,Kohan SA,Leung B,Bouchard LS

    更新日期:2013-01-01 00:00:00