当前位置： SCI文献检索 > JOURNAL OF ETHNOPHARMACOLOGY期刊下所有文献 > Cytotoxic ethnic Yao medicine Baizuan, leaves of Schisandra viridis A. C. Smith.

Cytotoxic ethnic Yao medicine Baizuan, leaves of Schisandra viridis A. C. Smith.

Abstract：

ETHNOPHARMACOLOGICAL RELEVANCE:The ethnic Chinese Yao medicine Baizuan, which are the leaves of Schisandra viridis A. C. Smith, is traditionally used, in combination with other herbs, to soften hard lumps and dispel nodes in the treatment of cancer, however, this property has not been well studied with a clear indication of the active principles. AIM OF THE STUDY:The experiments were carried out to investigate the cytotoxic activity of the extracts and to identify the active principles from the extract, which could support the traditional application of treating cancer. MATERIALS AND METHODS:Dried and ground plant material was extracted with water and ethanol and further purified by HPLC. The cytotoxicity of the extracts, fractions and pure compounds were evaluated for their abilities to inhibit the proliferation of breast cancer cells MCF7 and tongue cancer cells CAL27. The cytotoxicity of the pure compounds were also tested against Human Embryonic Kidney cell line HEK293. RESULTS:Both aqueous and ethanol extracts showed activities against MCF7 and CAL27 cancer cells. Bioassay-guided fractionation and purification of the extracts resulted in six active principles, including five dibenzocyclooctene lignans namely gomisin H (1), schisandrin (2), angeloylgomisin H (3), (+)-gomisin M2 (4) and (-)-rubschisandrin (5), and one triterpenoid, schisanol (6). Compounds 1-3 showed moderate cytotoxic activities with IC50 values ranging from 100 to 200µg/mL against MCF7 and CAL27 cell lines. Dioxane containing lignans 4-5 and triterpenoid 6 were 10 times more active with IC50 values of 14.5, 13.4, 10.6µg/mL against MCF7, and 21.2, 17.9, 11.7µg/mL against CAL27, respectively. Compounds 1-6 also showed cytotoxicity against HEK293 with IC50 values ranging from 10 to 150µg/mL, respectively. CONCLUSIONS:The traditional extraction protocol using boiled water afforded three moderately active lignans 1-3. Ethanol extraction, which is widely used in the preparation of herbal remedies in China, yielded three additional active compounds 4-5 with more potent activities. These results provided a rationale for the traditional application of the ethnic Yao medicine Baizuan in the treatment of cancer.

journal_name

J Ethnopharmacol

journal_title

Journal of ethnopharmacology

authors

Hou X,Deng J,Zhang Q,Wang D,Kennedy D,Quinn RJ,Feng Y

doi

10.1016/j.jep.2016.09.016

subject

Has Abstract

pub_date

2016-12-24 00:00:00

pages

146-152

eissn

0378-8741

issn

1872-7573

pii

S0378-8741(16)30745-0

journal_volume

194

pub_type

杂志文章

在线工具

Cytotoxic ethnic Yao medicine Baizuan, leaves of Schisandra viridis A. C. Smith.