Abstract:
ETHNOPHARMACOLOGICAL RELEVANCE:Danshen is a crude herbal drug isolated from dried roots of Salvia miltiorrhiza Bunge. This plant is widely used in oriental medicine for the treatment of cardiovascular and cerebrovascular diseases. The supercritical CO2 extract from Danshen (SCED) (57.85%, 5.67% and 4.55% for tanshinone IIA, tanshinone I and cryptotanshinone respectively) was studied in this article, whose potential molecular mechanism remains unclear, especially in anti-thrombosis. AIM OF THE STUDY:The present study was designed to observe the protective effect of SCED on ischemic stroke in rats and to explore the underlying anti-thrombosis mechanism. MATERIALS AND METHODS:Following induction of cerebral ischemia in rats by permanent middle cerebral artery occlusion (pMCAO). Neurological defect score, cerebral blood flow, infarct size, and brain edema were measured to evaluate the injury. Arteriovenous shunt thrombosis model and adenosine 5'-diphosphate (ADP) induced acute pulmonary embolism model were conducted to estimate the antithrombotic effect of SCED. In order to investigate the effects of SCED on platelet aggregation, rat platelet-rich-plasma (PRP) were incubated with SCED prior to the addition of the stimuli (ADP or 9, 11-dideoxy-11α, 9α-epoxymethanoprostaglandin F2α (U46619)). Aggregation was monitored in a light transmission aggregometer. Inhibitory effect of SCED on thromboxane A2 (TXA2) release was detected by ELISA kit. Phospholipase C (PLC)/ Protein kinase C (PKC) signaling pathway was analyzed by a Western blot technique. The effect of the SCED was also studied in vivo on bleeding time in mice. RESULTS:SCED improved the neurological defect score, increased cerebral blood flow, reduced infarct size and alleviated brain edema in rats exposed to pMCAO. After administration of SCED, thrombosis formation in arteriovenous shunt was inhibited and recovery time in pulmonary embolism was shortened. The inhibitory effect of SCED on platelet activation was further confirmed by TXB2 ELISA kit and Western blot analysis of PLC/PKC signaling pathway. CONCLUSIONS:SCED attenuates cerebral ischemic injury. The possible mechanism is that SCED inhibits thrombosis formation, platelet aggregation and activation of PLC/PKC pathway. On this basis, this new extract could be a promising agent to inhibit thrombosis formation and protect against cerebral ischemia injury.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Fei YX,Wang SQ,Yang LJ,Qiu YY,Li YZ,Liu WY,Xi T,Fang WR,Li YMdoi
10.1016/j.jep.2017.06.023subject
Has Abstractpub_date
2017-07-31 00:00:00pages
57-66eissn
0378-8741issn
1872-7573pii
S0378-8741(16)31963-8journal_volume
207pub_type
杂志文章abstract::Traditional livestock healers from Samburu and Turkana communities in Samburu District of Kenya were identified and recruited for participation in this study. Sheep with mixed gastrointestinal helminthosis were purchased from nomadic livestock owners in the area. Three herbal anthelmintic remedies, viz. Myrsine africa...
journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Traditional Chinese herbal medicine (CHM) has a long-history for treatment of various human diseases including tumors, infection, autoimmune diseases in Asian countries, especially in China, Japan, Korea and India. CHM was traditionally used as water extracts and many Chinese herbs were c...
journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2015-03-13 00:00:00
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journal_title:Journal of ethnopharmacology
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更新日期:2006-01-03 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2017-03-22 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.01.004
更新日期:2009-03-18 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
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更新日期:2021-02-10 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2019-05-10 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.10.060
更新日期:2016-12-24 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2017.07.034
更新日期:2017-09-14 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(00)00345-7
更新日期:2001-03-03 00:00:00
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journal_title:Journal of ethnopharmacology
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pub_type: 杂志文章
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更新日期:2016-07-21 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
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更新日期:2002-08-01 00:00:00
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journal_title:Journal of ethnopharmacology
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更新日期:1982-09-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.03.019
更新日期:2010-05-27 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2004.03.030
更新日期:2004-07-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(99)00181-6
更新日期:2000-07-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.06.024
更新日期:2009-09-07 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2017.01.027
更新日期:2017-02-23 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,收录出版
doi:10.1016/j.jep.2013.12.032
更新日期:2014-02-12 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(01)00379-8
更新日期:2002-02-01 00:00:00
abstract::Field enquiries on the plants used to treat diseases in villages of Seberida Municipality indicated that a large number of plant species (at least 100) are being used in therapy. Many of the uses, however, are magical in nature. Those in which a cause-effect relationship may be established (56) are presented in this p...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/0378-8741(91)90007-z
更新日期:1991-02-01 00:00:00
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journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2021.113894
更新日期:2021-01-28 00:00:00