Fluorometric Measurement of Individual Stomata Activity and Transpiration via a "Brush-on", Water-Responsive Polymer.

Abstract:

:Much of atmospheric water originates from transpiration, the process by which plants release H2O from pores, known as stomata, that simultaneously intake CO2 for photosynthesis. Controlling stomatal aperture can regulate the extent of water transport in response to dynamic environmental factors including osmotic stress, temperature, light, and wind. While larger leaf regions are often examined, the extent of water vapor release from individual stomata remains unexplored. Using a "brush-on" sensing material, we can now assess transpiration using a water-responsive, polydiacetylene-based coating on the leaves surfaces. By eliciting a fluorometric signal to passing water vapor, we obtained information regarding the activity of individual stomata. In this demonstration, our results prove that this coating can identify the proportion of active stomata and the extent of transpirational diffusion of water in response to different conditions.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Seo M,Park DH,Lee CW,Jaworski J,Kim JM

doi

10.1038/srep32394

subject

Has Abstract

pub_date

2016-08-31 00:00:00

pages

32394

issn

2045-2322

pii

srep32394

journal_volume

6

pub_type

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