当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens.

Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens.

Abstract：

:Results from live cell imaging of fluorescently tagged Cellulose Synthase (CESA) proteins in Cellulose Synthesis Complexes (CSCs) have enhanced our understanding of cellulose biosynthesis, including the mechanisms of action of cellulose synthesis inhibitors. However, this method has been applied only in Arabidopsis thaliana and Brachypodium distachyon thus far. Results from freeze fracture electron microscopy of protonemal filaments of the moss Funaria hygrometrica indicate that a cellulose synthesis inhibitor, 2,6-dichlorobenzonitrile (DCB), fragments CSCs and clears them from the plasma membrane. This differs from Arabidopsis, in which DCB causes CSC accumulation in the plasma membrane and a different cellulose synthesis inhibitor, isoxaben, clears CSCs from the plasma membrane. In this study, live cell imaging of the moss Physcomitrella patens indicated that DCB and isoxaben have little effect on protonemal growth rates, and that only DCB causes tip rupture. Live cell imaging of mEGFP-PpCESA5 and mEGFP-PpCESA8 showed that DCB and isoxaben substantially reduced CSC movement, but had no measureable effect on CSC density in the plasma membrane. These results suggest that DCB and isoxaben have similar effects on CSC movement in P. patens and Arabidopsis, but have different effects on CSC intracellular trafficking, cell growth and cell integrity in these divergent plant lineages.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Tran ML,McCarthy TW,Sun H,Wu SZ,Norris JH,Bezanilla M,Vidali L,Anderson CT,Roberts AW

doi

10.1038/s41598-017-18994-4

subject

Has Abstract

pub_date

2018-01-15 00:00:00

pages

735

issue

1

issn

2045-2322

pii

10.1038/s41598-017-18994-4

journal_volume

8

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • The hazardous 2017-2019 surge and river damming by Shispare Glacier, Karakoram.

    abstract：:In 2017-2019 a surge of Shispare Glacier, a former tributary of the once larger Hasanabad Glacier (Hunza region), dammed the proglacial river of Muchuhar Glacier, which formed an ice-dammed lake and generated a small Glacial Lake Outburst Flood (GLOF). Surge movement produced the highest recorded Karakoram glacier sur...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-61277-8

    authors： Bhambri R,Watson CS,Hewitt K,Haritashya UK,Kargel JS,Pratap Shahi A,Chand P,Kumar A,Verma A,Govil H

    更新日期：2020-03-13 00:00:00

  • Transitions to sustainable management of phosphorus in Brazilian agriculture.

    abstract：:Brazil's large land base is important for global food security but its high dependency on inorganic phosphorus (P) fertilizer for crop production (2.2 Tg rising up to 4.6 Tg in 2050) is not a sustainable use of a critical and price-volatile resource. A new strategic analysis of current and future P demand/supply concl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-20887-z

    authors： Withers PJA,Rodrigues M,Soltangheisi A,de Carvalho TS,Guilherme LRG,Benites VM,Gatiboni LC,de Sousa DMG,Nunes RS,Rosolem CA,Andreote FD,Oliveira A Jr,Coutinho ELM,Pavinato PS

    更新日期：2018-02-07 00:00:00

  • The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models.

    abstract：:Prolactin (PRL) is an anterior pituitary hormone with a broad range of functions. Its ability to stimulate lactogenesis, maternal behavior, growth and development, osmoregulation, and epithelial ion transport has been reported in many vertebrates. In our present study, we have targeted the zebrafish prl locus via tran...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep18597

    authors： Shu Y,Lou Q,Dai Z,Dai X,He J,Hu W,Yin Z

    更新日期：2016-01-04 00:00:00

  • Scalable long read self-correction and assembly polishing with multiple sequence alignment.

    abstract：:Third-generation sequencing technologies allow to sequence long reads of tens of kbp, that are expected to solve various problems. However, they display high error rates, currently capped around 10%. Self-correction is thus regularly used in long reads analysis projects. We introduce CONSENT, a new self-correction met...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-80757-5

    authors： Morisse P,Marchet C,Limasset A,Lecroq T,Lefebvre A

    更新日期：2021-01-12 00:00:00

  • Molecular characterization of colorectal adenomas with and without malignancy reveals distinguishing genome, transcriptome and methylome alterations.

    abstract：:The majority of colorectal cancer (CRC) arises from precursor lesions known as polyps. The molecular determinants that distinguish benign from malignant polyps remain unclear. To molecularly characterize polyps, we utilized Cancer Adjacent Polyp (CAP) and Cancer Free Polyp (CFP) patients. CAPs had tissues from the res...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21525-4

    authors： Druliner BR,Wang P,Bae T,Baheti S,Slettedahl S,Mahoney D,Vasmatzis N,Xu H,Kim M,Bockol M,O'Brien D,Grill D,Warner N,Munoz-Gomez M,Kossick K,Johnson R,Mouchli M,Felmlee-Devine D,Washechek-Aletto J,Smyrk T,Oberg A,

    更新日期：2018-02-16 00:00:00

  • High-hole mobility polycrystalline Ge on an insulator formed by controlling precursor atomic density for solid-phase crystallization.

    abstract：:High-carrier mobility semiconductors on insulators are essential for fabricating advanced thin-film transistors, allowing for three-dimensional integrated circuits or high-performance mobile terminals. We investigate the low-temperature (375-450 °C) solid-phase crystallization (SPC) of Ge on a glass substrate, focusin...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17273-6

    authors： Toko K,Yoshimine R,Moto K,Suemasu T

    更新日期：2017-12-05 00:00:00

  • CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method.

    abstract：:Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is a powerful tool to manipulate the genome with extraordinary simplicity and speed. To generate genetically modified animals, CRISPR/Cas9-mediated genome editing is typically accomplished by microinjectio...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep42244

    authors： Ma X,Chen C,Veevers J,Zhou X,Ross RS,Feng W,Chen J

    更新日期：2017-02-08 00:00:00

  • Differences in the diagnosis and management of pyriform sinus fistula between newborns and children.

    abstract：:Pyriform sinus fistula (PSF) is a rare congenital entity. We hypothesized that clinical features, diagnosis and treatment may differ between newborns and children. 190 patients diagnosed with PSF were divided into two groups: neonatal (n = 15) and childhood (n = 175). The medical records including demographic and clin...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-55050-9

    authors： Sheng Q,Lv Z,Xu W,Liu J

    更新日期：2019-12-06 00:00:00

  • Stochastic kinetics reveal imperative role of anisotropic interfacial tension to determine morphology and evolution of nucleated droplets in nematogenic films.

    abstract：:For isotropic fluids, classical nucleation theory predicts the nucleation rate, barrier height and critical droplet size by ac- counting for the competition between bulk energy and interfacial tension. The nucleation process in liquid crystals is less understood. We numerically investigate nucleation in monolayered ne...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep40059

    authors： Bhattacharjee AK

    更新日期：2017-01-05 00:00:00

  • Men increase contributions to a public good when under sexual competition.

    abstract：:Why humans cooperate in large groups and with non-kin remains a puzzle for researchers across the natural and social sciences. Investigating whether cooperation is sexually selected could contribute to an understanding of the evolution of human cooperation. Competition for access to mates could indeed select for coope...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep29819

    authors： Tognetti A,Dubois D,Faurie C,Willinger M

    更新日期：2016-07-14 00:00:00

  • The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.

    abstract：:The major human pathogen Streptococcus pneumoniae is a leading cause of disease and death worldwide. Pneumococcal biofilm formation within the nasopharynx leads to long-term colonization and persistence within the host. We have previously demonstrated that the capsular surface-associated pneumococcal serine rich repea...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep32371

    authors： Schulte T,Mikaelsson C,Beaussart A,Kikhney A,Deshmukh M,Wolniak S,Pathak A,Ebel C,Löfling J,Fogolari F,Henriques-Normark B,Dufrêne YF,Svergun D,Nygren PÅ,Achour A

    更新日期：2016-09-01 00:00:00

  • Strain engineering of transverse electric and transverse magnetic mode of material gain in GeSn/SiGeSn quantum wells.

    abstract：:8-band k · p Hamiltonian together with envelope function approximation and planewave expansion method are applied to calculate the electronic band structure and material gain for Ge1-wSnw/SiyGe1-x-ySnx/Ge1-wSnw quantum wells (QWs) grown on virtual Ge1-zSnz substrates integrated with Si platform. It is clearly shown ho...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-40146-z

    authors： Mączko HS,Kudrawiec R,Gladysiewicz M

    更新日期：2019-03-01 00:00:00

  • Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization.

    abstract：:It is known that ribosomal RNA (rRNA) synthesis is regulated by cellular energy and proliferation status. In this study, we investigated rRNA gene transcription in response to cytoskeletal stress. Our data revealed that the cell shape constrained by isotropic but not elongated micropatterns in HeLa cells led to a sign...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep28685

    authors： Wu TH,Kuo YY,Lee HH,Kuo JC,Ou MH,Chang ZF

    更新日期：2016-06-28 00:00:00

  • Kup-mediated Cs+ uptake and Kdp-driven K+ uptake coordinate to promote cell growth during excess Cs+ conditions in Escherichia coli.

    abstract：:The physiological effects of caesium (Cs) on living cells are poorly understood. Here, we examined the physiological role of Cs+ on the activity of the potassium transporters in E. coli. In the absence of potassium (K+), Kup-mediated Cs+ uptake partially supported cell growth, however, at a much lower rate than with s...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02164-7

    authors： Tanudjaja E,Hoshi N,Su YH,Hamamoto S,Uozumi N

    更新日期：2017-05-18 00:00:00

  • Spin-dependent manipulating of vector beams by tailoring polarization.

    abstract：:We examine the spin-dependent manipulating of vector beams by tailoring the inhomogeneous polarization. The spin-dependent manipulating is attributed to the spin-dependent phase gradient in vector beams, which can be regarded as the intrinsic feature of inhomogeneous polarization. The desired polarization can be obtai...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep34276

    authors： Zhou J,Zhang W,Liu Y,Ke Y,Liu Y,Luo H,Wen S

    更新日期：2016-09-28 00:00:00

  • ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain.

    abstract：:The role of the actin cytoskeleton in relation to mitochondria function and dynamics is only recently beginning to be recognized. Myo19 is an actin-based motor that is bound to the outer mitochondrial membrane and promotes the localization of mitochondria to filopodia in response to glucose starvation. However, how gl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-11002-9

    authors： Shneyer BI,Ušaj M,Wiesel-Motiuk N,Regev R,Henn A

    更新日期：2017-09-14 00:00:00

  • Kaempferol stimulates gene expression of low-density lipoprotein receptor through activation of Sp1 in cultured hepatocytes.

    abstract：:A high level of plasma low-density lipoprotein (LDL) cholesterol is considered a risk factor for atherosclerosis. Because the hepatic LDL receptor (LDLR) is essential for clearing plasma LDL cholesterol, activation of LDLR is a promising therapeutic target for patients with atherosclerotic disease. Here we demonstrate...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep24940

    authors： Ochiai A,Miyata S,Iwase M,Shimizu M,Inoue J,Sato R

    更新日期：2016-04-25 00:00:00

  • Antiviral activity of chlorogenic acid against influenza A (H1N1/H3N2) virus and its inhibition of neuraminidase.

    abstract：:Lonicera japonica Thunb, rich in chlorogenic acid (CHA), is used for viral upper respiratory tract infection treatment caused by influenza virus, parainfluenza virus, and respiratory syncytial virus, ect in China. It was reported that CHA reduced serum hepatitis B virus level and death rate of influenza virus-infected...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45723

    authors： Ding Y,Cao Z,Cao L,Ding G,Wang Z,Xiao W

    更新日期：2017-04-10 00:00:00

  • Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration.

    abstract：:Nuclear distribution element-like 1 (Ndel1) plays pivotal roles in diverse biological processes and is implicated in the pathogenesis of multiple neurodevelopmental disorders. Ndel1 function by regulating microtubules and intermediate filaments; however, its functional link with the actin cytoskeleton is largely unkno...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep31827

    authors： Hong JH,Kwak Y,Woo Y,Park C,Lee SA,Lee H,Park SJ,Suh Y,Suh BK,Goo BS,Mun DJ,Sanada K,Nguyen MD,Park SK

    更新日期：2016-08-22 00:00:00

  • Author Correction: Some coral diseases track climate oscillations in the Caribbean.

    abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...

    journal_title：Scientific reports

    pub_type： 已发布勘误

    doi：10.1038/s41598-018-23087-x

    authors： Randall CJ,van Woesik R

    更新日期：2018-03-19 00:00:00

  • Synchronization to a bouncing ball with a realistic motion trajectory.

    abstract：:Daily music experience involves synchronizing movements in time with a perceived periodic beat. It has been established for over a century that beat synchronization is less stable for the visual than for the auditory modality. This auditory advantage of beat synchronization gives rise to the hypotheses that the neural...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep11974

    authors： Gan L,Huang Y,Zhou L,Qian C,Wu X

    更新日期：2015-07-07 00:00:00

  • Underground hibernation in a primate.

    abstract：:Hibernation in mammals is a remarkable state of heterothermy wherein metabolic rates are reduced, core body temperatures reach ambient levels, and key physiological functions are suspended. Typically, hibernation is observed in cold-adapted mammals, though it has also been documented in tropical species and even prima...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep01768

    authors： Blanco MB,Dausmann KH,Ranaivoarisoa JF,Yoder AD

    更新日期：2013-01-01 00:00:00

  • Effect of ionic liquid treatment on the ultrastructural and topochemical features of compression wood in Japanese cedar (Cryptomeria japonica).

    abstract：:The morphological and topochemical changes in wood tissues in compression wood of Japanese cedar (Cryptomeria japonica) upon treated with two types of ionic liquids, 1-ethyl-3-methylimidazolium chloride ([C2mim][Cl]) and 1-ethylpyridinium bromide ([EtPy][Br]) were investigated. Compression wood tracheids were swollen ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep30147

    authors： Kanbayashi T,Miyafuji H

    更新日期：2016-07-18 00:00:00

  • Rotating magnetocaloric effect and unusual magnetic features in metallic strongly anisotropic geometrically frustrated TmB4.

    abstract：:We have investigated the rotating magnetocaloric effect (R-MCE) of TmB4 - an anisotropic magnetic system with geometrical frustration of Shastry-Sutherland type. The R-MCE was obtained from detailed temperature dependencies of heat capacity in various magnetic fields of a single crystalline sample for crystal axes ori...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-29399-2

    authors： Orendáč M,Gabáni S,Gažo E,Pristáš G,Shitsevalova N,Siemensmeyer K,Flachbart K

    更新日期：2018-07-19 00:00:00

  • Cross section measurements of 151Eu(3He,5n) reaction: new opportunities for medical alpha emitter 149Tb production.

    abstract：:Method for production of alpha emitter 149Tb by irradiation of 151Eu with 70 MeV 3He nuclei is proposed. For the first time, the cross sections for the formation of isotopes 149,150,151,152Tb were measured experimentally using a stack foil technique in the 3He particles energy range 70 → 12 MeV. The thick target yield...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-57436-6

    authors： Moiseeva AN,Aliev RA,Unezhev VN,Zagryadskiy VA,Latushkin ST,Aksenov NV,Gustova NS,Voronuk MG,Starodub GY,Ogloblin AA

    更新日期：2020-01-16 00:00:00

  • Tailoring tobacco hairy root metabolism for the production of stilbenes.

    abstract：:Tobacco hairy root (HR) cultures, which have been widely used for the heterologous production of target compounds, have an innate capacity to bioconvert exogenous t-resveratrol (t-R) into t-piceatannol (t-Pn) and t-pterostilbene (t-Pt). We established genetically engineered HR carrying the gene encoding stilbene synth...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-18330-w

    authors： Hidalgo D,Georgiev M,Marchev A,Bru-Martínez R,Cusido RM,Corchete P,Palazon J

    更新日期：2017-12-21 00:00:00

  • Allocation of photosynthesized carbon in an intensively farmed winter wheat-soil system as revealed by 14CO2 pulse labelling.

    abstract：:Understanding the rhizodeposited carbon (C) dynamics of winter wheat (Triticum aestivum L.), is crucial for soil fertility and C sequestration. Pot-grown winter wheat was pulse labelled with 14CO2 at the key growth stages. 14C in the shoots, roots and soil was measured at 5 or 2 days after 14C-labelling (DAL 5/2) at e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21547-y

    authors： Sun Z,Chen Q,Han X,Bol R,Qu B,Meng F

    更新日期：2018-02-16 00:00:00

  • A practical method for multimodal registration and assessment of whole-brain disease burden using PET, MRI, and optical imaging.

    abstract：:Many neurological diseases present with substantial genetic and phenotypic heterogeneity, making assessment of these diseases challenging. This has led to ineffective treatments, significant morbidity, and high mortality rates for patients with neurological diseases, including brain cancers and neurodegenerative disor...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-74459-1

    authors： Scarpelli ML,Healey DR,Mehta S,Kodibagkar VD,Quarles CC

    更新日期：2020-10-14 00:00:00

  • Occurrence of fibrates and their metabolites in source and drinking water in Shanghai and Zhejiang, China.

    abstract：:Fibrates, which are widely used lipidaemic-modulating drugs, are emerging environmental pollutants. However, fibrate concentrations in the environment have not been thoroughly surveyed. Here, we determined concentrations of the most commonly used fibrates and their metabolites in source water and drinking water sample...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45931

    authors： Ido A,Hiromori Y,Meng L,Usuda H,Nagase H,Yang M,Hu J,Nakanishi T

    更新日期：2017-04-12 00:00:00

  • An automated method for identifying an independent component analysis-based language-related resting-state network in brain tumor subjects for surgical planning.

    abstract：:As a noninvasive and "task-free" technique, resting-state functional magnetic resonance imaging (rs-fMRI) has been gradually applied to pre-surgical functional mapping. Independent component analysis (ICA)-based mapping has shown advantage, as no a priori information is required. We developed an automated method for i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14248-5

    authors： Lu J,Zhang H,Hameed NUF,Zhang J,Yuan S,Qiu T,Shen D,Wu J

    更新日期：2017-10-23 00:00:00