Novel Variations in Native Ethiopian Goat breeds PRNP Gene and Their Potential Effect on Prion Protein Stability.

Abstract:

:Scrapie is a lethal neurodegenerative disease of sheep and goats caused by the misfolding of the prion protein. Variants such as M142, D145, S146, H154, Q211, and K222 were experimentally found to increase resistance or extend scrapie incubation period in goats. We aimed to identify polymorphisms in the Afar and Arsi-Bale goat breeds of Ethiopia and computationally assess the effect of variants on prion protein stability. In the present study, four non-synonymous novel polymorphisms G67S, W68R, G69D, and R159H in the first octapeptide repeat and the highly conserved C-terminus globular domain of goat PrP were detected. The resistant genotype, S146, was detected in >50% of the present population. The current study population showed a genetic diversity in Ethiopian goat breeds. In the insilico analysis, the R68 variant was predicted to increase stability while S67, D69, and H159 decrease the stability of prion protein. The new variants in the octapeptide repeat motif were predicted to decrease amyloidogenicity but H159 increased the hotspot sequence amyloidogenic propensity. These novel variants could be the source of conformational flexibility that may trigger the gain or loss of function by prion protein. Further experimental study is required to depict the actual effects of variants on prion protein stability.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Teferedegn EY,Yaman Y,Ün C

doi

10.1038/s41598-020-63874-z

subject

Has Abstract

pub_date

2020-04-24 00:00:00

pages

6953

issue

1

issn

2045-2322

pii

10.1038/s41598-020-63874-z

journal_volume

10

pub_type

杂志文章
  • Retraction Note: Sorcin Predicts Poor Prognosis and Promotes Metastasis by Facilitating Epithelial-mesenchymal Transition in Hepatocellular Carcinoma.

    abstract::This paper has been retracted at the request of the authors. ...

    journal_title:Scientific reports

    pub_type: 撤回出版物

    doi:10.1038/s41598-018-29892-8

    authors: Lei X,Liang Y,Chen J,Xiao S,Lei J,Li J,Duanmu J,Jiang Q,Liu D,Tang C,Li T

    更新日期:2018-08-02 00:00:00

  • 8-Oxoguanine accumulation in mitochondrial DNA causes mitochondrial dysfunction and impairs neuritogenesis in cultured adult mouse cortical neurons under oxidative conditions.

    abstract::Oxidative stress and mitochondrial dysfunction are implicated in aging-related neurodegenerative disorders. 8-Oxoguanine (8-oxoG), a common oxidised base lesion, is often highly accumulated in brains from patients with neurodegenerative disorders. MTH1 hydrolyses 8-oxo-2'-deoxyguanosine triphosphate (8-oxo-dGTP) to 8-...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22086

    authors: Leon J,Sakumi K,Castillo E,Sheng Z,Oka S,Nakabeppu Y

    更新日期:2016-02-25 00:00:00

  • Gram Negative Bacterial Inflammation Ameliorated by the Plasma Protein Beta 2-Glycoprotein I.

    abstract::Lipopolysaccharide (LPS) is a major component of the outer wall of gram negative bacteria. In high doses LPS contributes to the inflammation in gram negative sepsis, and in low doses contributes to the low grade inflammation characteristic of the metabolic syndrome. We wanted to assess the role of beta2-glycoprotein I...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep33656

    authors: Zhou S,Chen G,Qi M,El-Assaad F,Wang Y,Dong S,Chen L,Yu D,Weaver JC,Beretov J,Krilis SA,Giannakopoulos B

    更新日期:2016-09-27 00:00:00

  • Tomatoes protect against development of UV-induced keratinocyte carcinoma via metabolomic alterations.

    abstract::Prolonged tomato consumption can mitigate ultraviolet (UV) light induced sunburn via unknown mechanisms. Dietary carotenoids distributed to skin are hypothesized to protect skin against UV-induced damage, although other phytochemicals may play a role. We hypothesize that tomato consumption would protect against skin c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05568-7

    authors: Cooperstone JL,Tober KL,Riedl KM,Teegarden MD,Cichon MJ,Francis DM,Schwartz SJ,Oberyszyn TM

    更新日期:2017-07-11 00:00:00

  • Genotyping-by-sequencing of three mapping populations for identification of candidate genomic regions for resistance to sterility mosaic disease in pigeonpea.

    abstract::Sterility mosaic disease (SMD) is one of the serious production constraints that may lead to complete yield loss in pigeonpea. Three mapping populations including two recombinant inbred lines and one F2, were used for phenotyping for SMD resistance at two locations in three different years. Genotyping-by-sequencing ap...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01535-4

    authors: Saxena RK,Kale SM,Kumar V,Parupali S,Joshi S,Singh V,Garg V,Das RR,Sharma M,Yamini KN,Ghanta A,Rathore A,Sameerkumar CV,Saxena KB,Varshney RK

    更新日期:2017-05-12 00:00:00

  • Association between Hepatitis B Virus Infection and Metabolic Syndrome in Southwest China: A Cross-sectional Study.

    abstract::The correlation between hepatitis B virus (HBV) infection and metabolic syndrome (MetS) remains to be clarified. In this study, we explored this association in a large population in Southwest China. This was a cross-sectional study, with pooled adult health data. Multivariate logistic regression analysis, controlling ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62609-4

    authors: Yan LB,Liao J,Han N,Zhou LY,Wang XE,Wang YJ,Tang H

    更新日期:2020-04-21 00:00:00

  • Controllable design of super-oscillatory lenses with multiple sub-diffraction-limit foci.

    abstract::The conventional multifocal optical elements cannot precisely control the focal number, spot size, as well as the energy distribution in between. Here, the binary amplitude-type super-oscillatory lens (SOL) is utilized, and a robust and universal optimization method based on the vectorial angular spectrum (VAS) theory...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-01492-y

    authors: Li M,Li W,Li H,Zhu Y,Yu Y

    更新日期:2017-05-02 00:00:00

  • Characteristics of pulsed runoff-erosion events under typical rainstorms in a small watershed on the Loess Plateau of China.

    abstract::The pulsed events of rainstorm erosion on the Loess Plateau are well-known, but little information is available concerning the characteristics of superficial soil erosion processes caused by heavy rainstorms at the watershed scale. This study statistically evaluated characteristics of pulsed runoff-erosion events base...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22045-x

    authors: Wu L,Jiang J,Li GX,Ma XY

    更新日期:2018-02-27 00:00:00

  • Effects of rice straw and rice straw ash on rice growth and α-diversity of bacterial community in rare-earth mining soils.

    abstract::Pot experiments were carried out to study the effects of rice straw (RS) and rice straw ash (RSA) on the growth of early rice and α-diversity of bacterial community in soils around rare earth mining areas of Xunwu and Xinfeng counties in South Jiangxi of China. The results showed that the exploitation of rare earth re...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-67160-w

    authors: Jin S,Jin W,Dong C,Bai Y,Jin D,Hu Z,Huang Y

    更新日期:2020-06-25 00:00:00

  • Plastic, nutrition and pollution; relationships between ingested plastic and metal concentrations in the livers of two Pachyptila seabirds.

    abstract::Naturally occurring metals and metalloids [metal(loid)s] are essential for the physiological functioning of wildlife; however, environmental contamination by metal(loid) and plastic pollutants is a health hazard. Metal(loid)s may interact with plastic in the environment and there is mixed evidence about whether plasti...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-75024-6

    authors: Roman L,Kastury F,Petit S,Aleman R,Wilcox C,Hardesty BD,Hindell MA

    更新日期:2020-10-22 00:00:00

  • Concurrent Chemotherapy of Malignant Glioma in Rats by Using Multidrug-Loaded Biodegradable Nanofibrous Membranes.

    abstract::Glioblastoma multiforme has a poor prognosis and is highly chemoresistant. In this study, we implanted biodegradable 1,3-bis[2-chloroethyl]-1-nitroso-urea-, irinotecan-, and cisplatin-eluting poly[(d,l)-lactide-co-glycolide] (BIC/PLGA) and virgin nanofibrous membranes on the brain surface of C6 glioma-bearing rats in ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep30630

    authors: Tseng YY,Huang YC,Yang TC,Yang ST,Liu SC,Chang TM,Kau YC,Liu SJ

    更新日期:2016-07-29 00:00:00

  • Evolution and Determinants of Lung Function until Late Infancy among Infants Born Preterm.

    abstract::To investigate the evolution of lung function in preterm infants with and without bronchopulmonary dysplasia (BPD) and to determine the perinatal characteristics associated with indexes of lung function in later infancy. Longitudinal lung function assessments were performed at approximately 6, 12, 18, and 24 months of...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-57359-x

    authors: Lai SH,Chiang MC,Chu SM,Hsu JF,Yao TC,Tsai MH,Hua MC,Chiu CY,Yeh KW,Huang JL,Liao SL

    更新日期:2020-01-16 00:00:00

  • Enhancing Morphology and Separation Performance of Polyamide 6,6 Membranes By Minimal Incorporation of Silver Decorated Graphene Oxide Nanoparticles.

    abstract::Nanomaterials can be incorporated in the synthesis of membrane to obtain mixed-matrix membrane with marked improvement in properties and performance. However, stability and dispersion of the nanomaterials in the membrane matrix, as well as the need to use high ratio of nanomaterials for obvious improvement of membrane...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-38060-x

    authors: Mahmoudi E,Ng LY,Ang WL,Chung YT,Rohani R,Mohammad AW

    更新日期:2019-02-04 00:00:00

  • The T cell receptor resides in ordered plasma membrane nanodomains that aggregate upon patching of the receptor.

    abstract::Two related models for T cell signalling initiation suggest either that T cell receptor (TCR) engagement leads to its recruitment to ordered membrane domains, often referred to as lipid rafts, where signalling molecules are enriched or that ordered TCR-containing membrane nanodomains coalesce upon TCR engagement. That...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10082

    authors: Dinic J,Riehl A,Adler J,Parmryd I

    更新日期:2015-05-08 00:00:00

  • Author Correction: Evolutionary and expression analysis of CAMTA gene family in Nicotiana tabacum yielded insights into their origin, expansion and stress responses.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-60470-z

    authors: Kakar KU,Nawaz Z,Cui Z,Cao P,Jin J,Shu Q,Ren X

    更新日期:2020-03-10 00:00:00

  • Ocular Manifestations of Leukemia and Results of Treatment with Intravitreal Methotrexate.

    abstract::Ocular involvement in leukemia is considered rare. Ocular symptoms can be the presenting signs of leukemia, they can appear after diagnosis has been established, or they can be the first manifestation of a relapse after remission. We report, to the best of our knowledge for the first time, the ocular manifestation of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58654-8

    authors: Vishnevskia-Dai V,Sella King S,Lekach R,Fabian ID,Zloto O

    更新日期:2020-02-06 00:00:00

  • Clinical characteristics of branch retinal vein occlusion with increased retinal haemorrhage during treatment for macular oedema.

    abstract::This study was performed to evaluate clinical characteristics of branch retinal vein occlusion (BRVO) patients with increased retinal haemorrhage during macular oedema (MO) treatment. Patients were divided into increased and non-increased retinal haemorrhage groups. The former group was sub-divided based on the degree...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-67395-7

    authors: Lee YH,Kim YC

    更新日期:2020-06-24 00:00:00

  • Lack of interleukin-33 and its receptor does not prevent calcipotriol-induced atopic dermatitis-like inflammation in mice.

    abstract::Current studies addressing the influence of interleukin-33 or its receptor (IL-33R/ST2) on development of atopic dermatitis-like inflammation in mice have reported conflicting results. We compared the response in single- and double-deficient IL-33-/-/ST2-/- C57BL/6J BomTac mice in the well-established calcipotriol-ind...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63410-z

    authors: Pietka W,Sundnes O,Hammarström C,Zucknick M,Khnykin D,Haraldsen G

    更新日期:2020-04-15 00:00:00

  • Integrated Proteomic and Metabolomic prediction of Term Preeclampsia.

    abstract::Term preeclampsia (tPE), ≥37 weeks, is the most common form of PE and the most difficult to predict. Little is known about its pathogenesis. This study aims to elucidate the pathogenesis and assess early prediction of tPE using serial integrated metabolomic and proteomic systems biology approaches. Serial first- (11-1...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15882-9

    authors: Bahado-Singh R,Poon LC,Yilmaz A,Syngelaki A,Turkoglu O,Kumar P,Kirma J,Allos M,Accurti V,Li J,Zhao P,Graham SF,Cool DR,Nicolaides K

    更新日期:2017-11-23 00:00:00

  • Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer.

    abstract::Accumulating evidence indicates that cancer cells show specific alterations in phospholipid metabolism that contribute to tumour progression in several types of cancer, including colorectal cancer. Questions still remain as to what lipids characterize the outer edge of cancer tissues and whether those cancer outer edg...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29935

    authors: Hiraide T,Ikegami K,Sakaguchi T,Morita Y,Hayasaka T,Masaki N,Waki M,Sugiyama E,Shinriki S,Takeda M,Shibasaki Y,Miyazaki S,Kikuchi H,Okuyama H,Inoue M,Setou M,Konno H

    更新日期:2016-07-20 00:00:00

  • Cross comparison and prognostic assessment of breast cancer multigene signatures in a large population-based contemporary clinical series.

    abstract::Multigene expression signatures provide a molecular subdivision of early breast cancer associated with patient outcome. A gap remains in the validation of such signatures in clinical treatment groups of patients within population-based cohorts of unselected primary breast cancer representing contemporary disease stage...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-48570-x

    authors: Vallon-Christersson J,Häkkinen J,Hegardt C,Saal LH,Larsson C,Ehinger A,Lindman H,Olofsson H,Sjöblom T,Wärnberg F,Ryden L,Loman N,Malmberg M,Borg Å,Staaf J

    更新日期:2019-08-21 00:00:00

  • Molecular ecological network analysis reveals the effects of probiotics and florfenicol on intestinal microbiota homeostasis: An example of sea cucumber.

    abstract::Animal gut harbors diverse microbes that play crucial roles in the nutrition uptake, metabolism, and the regulation of host immune responses. The intestinal microbiota homeostasis is critical for health but poorly understood. Probiotics Paracoccus marcusii DB11 and Bacillus cereus G19, and antibiotics florfenicol did ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-05312-1

    authors: Yang G,Peng M,Tian X,Dong S

    更新日期:2017-07-06 00:00:00

  • Development of Spexin-based Human Galanin Receptor Type II-Specific Agonists with Increased Stability in Serum and Anxiolytic Effect in Mice.

    abstract::The novel neuropeptide spexin (SPX) was discovered to activate galanin receptor 2 (GALR2) and 3 (GALR3) but not galanin receptor 1 (GALR1). Although GALR2 is known to display a function, particularly in anxiety, depression, and appetite regulation, the further determination of its function would benefit from a more st...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep21453

    authors: Reyes-Alcaraz A,Lee YN,Son GH,Kim NH,Kim DK,Yun S,Kim DH,Hwang JI,Seong JY

    更新日期:2016-02-24 00:00:00

  • Dynamical complexity and computation in recurrent neural networks beyond their fixed point.

    abstract::Spontaneous activity found in neural networks usually results in a reduction of computational performance. As a consequence, artificial neural networks are often operated at the edge of chaos, where the network is stable yet highly susceptible to input information. Surprisingly, regular spontaneous dynamics in Neural ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21624-2

    authors: Marquez BA,Larger L,Jacquot M,Chembo YK,Brunner D

    更新日期:2018-02-20 00:00:00

  • Revisiting the concept of a symmetric index of agreement for continuous datasets.

    abstract::Quantifying how close two datasets are to each other is a common and necessary undertaking in scientific research. The Pearson product-moment correlation coefficient r is a widely used measure of the degree of linear dependence between two data series, but it gives no indication of how similar the values of these seri...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19401

    authors: Duveiller G,Fasbender D,Meroni M

    更新日期:2016-01-14 00:00:00

  • Clinical Implementation of Integrated Genomic Profiling in Patients with Advanced Cancers.

    abstract::DNA focused panel sequencing has been rapidly adopted to assess therapeutic targets in advanced/refractory cancer. Integrated Genomic Profiling (IGP) utilising DNA/RNA with tumour/normal comparisons in a Clinical Laboratory Improvement Amendments (CLIA) compliant setting enables a single assay to provide: therapeutic ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-016-0021-4

    authors: Borad MJ,Egan JB,Condjella RM,Liang WS,Fonseca R,Ritacca NR,McCullough AE,Barrett MT,Hunt KS,Champion MD,Patel MD,Young SW,Silva AC,Ho TH,Halfdanarson TR,McWilliams RR,Lazaridis KN,Ramanathan RK,Baker A,Aldrich J,

    更新日期:2016-12-23 00:00:00

  • Oscillometry in Chronic Obstructive Lung Disease: In vitro and in vivo evaluation of the impulse oscillometry and tremoflo devices.

    abstract::Impedance, or oscillometry, measurements of the respiratory system can generate information about the function of the respiratory system not possible with traditional spirometry. There are currently several instruments on the market using different perturbations. We have compared a new respiratory oscillometry instrum...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-48039-x

    authors: Lundblad LKA,Miletic R,Piitulainen E,Wollmer P

    更新日期:2019-08-12 00:00:00

  • Baseline IFN-γ and IL-10 expression in PBMCs could predict response to PD-1 checkpoint inhibitors in advanced melanoma patients.

    abstract::Anti-PD-1 antibodies revolutionized the treatment of advanced melanoma patients. However, one out of three do not respond to this therapy, with an overall poor prognosis. Identification of predictive biomarkers in patients receiving immune-based therapies is necessary for minimizing risk of toxicity and optimizing pat...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72711-2

    authors: Giunta EF,Barra G,De Falco V,Argenziano G,Napolitano S,Vitale P,Zanaletti N,Terminiello M,Martinelli E,Morgillo F,Ciardiello D,De Palma R,Ciardiello F,Troiani T

    更新日期:2020-10-19 00:00:00

  • Narrow band perfect absorber for maximum localized magnetic and electric field enhancement and sensing applications.

    abstract::Plasmonics offer an exciting way to mediate the interaction between light and matter, allowing strong field enhancement and confinement, large absorption and scattering at resonance. However, simultaneous realization of ultra-narrow band perfect absorption and electromagnetic field enhancement is challenging due to th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24063

    authors: Yong Z,Zhang S,Gong C,He S

    更新日期:2016-04-05 00:00:00

  • A simple, rapid, low-cost technique for naked-eye detection of urine-isolated TMPRSS2:ERG gene fusion RNA.

    abstract::The TMPRSS2:ERG gene fusion is one of a series of highly promising prostate cancer (PCa) biomarker alternatives to the controversial serum PSA. Current methods for detecting TMPRSS2:ERG are limited in terms of long processing time, high cost and the need for specialized equipment. Thus, there is an unmet need for less...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep30722

    authors: Koo KM,Wee EJ,Mainwaring PN,Trau M

    更新日期:2016-07-29 00:00:00