Abstract:
:Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune system diseases in order to contribute to improved understanding and evaluation of binding parameters for a variety of interactions between antigens and antibodies biochemically and clinically. Since the binding constants determination of an anti-protein dengue antibody (Ab) to a protein dengue antigen (Ag) is mostly complicated, the SPR technique aids a determination of binding parameters directly for a variety of particular dengue Ag_Ab interactions in the real-time. The study highlights the doctrine of real-time dengue Ag_Ab interaction kinetics as well as to determine the binding parameters that is performed with SPR technique. In addition, this article presents a precise prediction as a reference curve for determination of dengue sample concentration.
journal_name
Bioengineeredjournal_title
Bioengineeredauthors
Jahanshahi P,Wei Q,Jie Z,Ghomeishi M,Sekaran SD,Mahamd Adikan FRdoi
10.1080/21655979.2016.1223413subject
Has Abstractpub_date
2017-05-04 00:00:00pages
239-247issue
3eissn
2165-5979issn
2165-5987journal_volume
8pub_type
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