Analysis of miRNAs and their target genes associated with lipid metabolism in duck liver.

Abstract:

:Fat character is an important index in duck culture that linked to local flavor, feed cost and fat intake for costumers. Since the regulation networks in duck lipid metabolism had not been reported very clearly, we aimed to explore the potential miRNA-mRNA pairs and their regulatory roles in duck lipid metabolism. Here, Cherry-Valley ducks were selected and treated with/without 5% oil added in feed for 2 weeks, and then fat content determination was performed on. The data showed that the fat contents and the fatty acid ratios of C17:1 and C18:2 were up-regulated in livers of oil-added ducks, while the C12:0 ratio was down-regulated. Then 21 differential miRNAs, including 10 novel miRNAs, were obtain from the livers by sequencing, and 73 target genes involved in lipid metabolic processes of these miRNAs were found, which constituted 316 miRNA-mRNA pairs. Two miRNA-mRNA pairs including one novel miRNA and one known miRNA, N-miR-16020-FASN and gga-miR-144-ELOVL6, were selected to validate the miRNA-mRNA negative relation. And the results showed that N-mir-16020 and gga-miR-144 could respectively bind the 3'-UTRs of FASN and ELOVL6 to control their expressions. This study provides new sights and useful information for future research on regulation network in duck lipid metabolism.

journal_name

Sci Rep

journal_title

Scientific reports

authors

He J,Wang W,Lu L,Tian Y,Niu D,Ren J,Dong L,Sun S,Zhao Y,Chen L,Shen J,Li X

doi

10.1038/srep27418

subject

Has Abstract

pub_date

2016-06-08 00:00:00

pages

27418

issn

2045-2322

pii

srep27418

journal_volume

6

pub_type

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