Abstract：
:Cold environments dominate the Earth's biosphere and the resident microorganisms play critical roles in fulfilling global biogeochemical cycles. However, only few studies have examined the molecular basis of thermosensing; an ability that microorganisms must possess in order to respond to environmental temperature and regulate cellular processes. Two component regulatory systems have been inferred to function in thermal regulation of gene expression, but biochemical studies assessing these systems in Bacteria are rare, and none have been performed in Archaea or psychrophiles. Here we examined the LtrK/LtrR two component regulatory system from the Antarctic archaeon, Methanococcoides burtonii, assessing kinase and phosphatase activities of wild-type and mutant proteins. LtrK was thermally unstable and had optimal phosphorylation activity at 10 °C (the lowest optimum activity for any psychrophilic enzyme), high activity at 0 °C and was rapidly thermally inactivated at 30 °C. These biochemical properties match well with normal environmental temperatures of M. burtonii (0-4 °C) and the temperature this psychrophile is capable of growing at in the laboratory (-2 to 28 °C). Our findings are consistent with a role for LtrK in performing phosphotransfer reactions with LtrR that could lead to temperature-dependent gene regulation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Najnin T,Siddiqui KS,Taha T,Elkaid N,Kornfeld G,Curmi PM,Cavicchioli Rdoi
10.1038/srep24278subject
Has Abstractpub_date
2016-04-07 00:00:00pages
24278issn
2045-2322pii
srep24278journal_volume
6pub_type
杂志文章abstract：:Environmental pollutants as non-heritable factors are now recognized as triggers for multiple human inflammatory diseases involving T cells. We postulated that lipid antigen presentation mediated by cluster of differentiation 1 (CD1) proteins for T cell activation is susceptible to lipophilic environmental pollutants....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02229-7
更新日期：2017-05-18 00:00:00
abstract：:We demonstrate numerically and experimentally the enhancement of Surface Plasmon Resonance (SPR) sensing via dispersion engineering of the plasmonic response using plasmonic nanograting. Following their design and optimization, the plasmonic nanograting structures are fabricated using e-beam lithography and lift-off p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27023-x
更新日期：2018-06-13 00:00:00
abstract：:Visible indication based on the aggregation of colloidal nanoparticles (NPs) is highly advantageous for rapid on-site detection of biological entities, which even untrained persons can perform without specialized instrumentation. However, since the extent of aggregation should exceed a certain minimum threshold to pro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00456
更新日期：2012-01-01 00:00:00
abstract：:Amyloid formation is associated with various pathophysiological conditions like Alzheimer's and Parkinson's diseases as well as many useful functions. The hallmark of amyloid assemblies is a conformational transition of the constituent proteins into a β - sheet rich filament. Accounting for this conformational transit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21103
更新日期：2016-02-17 00:00:00
abstract：:The transport properties of films assembled from metal nanoclusters can be significantly different from the metals in their bulk or thin film forms due to quantum confinement effects and several competing energy and length scales. For a film composed of metal nanoclusters as its building blocks, the cluster size and t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07685
更新日期：2015-01-08 00:00:00
abstract：:Hormones are an important component in the regulatory networks guiding plant development. Cytokinins are involved in different physiological and developmental processes in plants. In the model plant Arabidopsis thaliana, cytokinin application during gynoecium development produces conspicuous phenotypes. On the other h...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25017-3
更新日期：2018-05-01 00:00:00
abstract：:Odors elicit complex patterns of activated olfactory sensory neurons. Knowing the complete olfactome, i.e. the responses in all sensory neurons for all relevant odorants, is desirable to understand olfactory coding. The DoOR project combines all available Drosophila odorant response data into a single consensus respon...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21841
更新日期：2016-02-25 00:00:00
abstract：:The zebrafish has the capacity to regenerate its heart after severe injury. While the function of a few genes during this process has been studied, we are far from fully understanding how genes interact to coordinate heart regeneration. To enable systematic insights into this phenomenon, we generated and integrated a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26822
更新日期：2016-05-31 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-24113-8
更新日期：2018-04-20 00:00:00
abstract：:SERS spectra excited at 785 nm of the bacteria Chlamydia trahomatis (elementary bodies, EB) and Neisseria gonorrheoae, the causative pathogens for the two most common sexually transmitted diseases (STD), chlamydia and gonorrhea, respectively, are reported. Although both are Gram-negative bacteria, the SERS signatures ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23562-5
更新日期：2018-03-26 00:00:00
abstract：:Cerebellar involvement in cognition, as well as in sensorimotor control, is increasingly recognized and is thought to depend on connections with the cerebral cortex. Anatomical investigations in animals and post-mortem humans have established that cerebro-cerebellar connections are contralateral to each other and incl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13079-8
更新日期：2017-10-09 00:00:00
abstract：:Adult Cebpb KO mice incisors present amelogenin-positive epithelium pearls, enamel and dentin allopathic hyperplasia, fewer Sox2-positive cells in labial cervical loop epitheliums, and reduced Sox2 expression in enamel epithelial stem cells. Thus, Cebpb acts upstream of Sox2 to regulate stemness. In this study, Cebpb ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23515-y
更新日期：2018-03-26 00:00:00
abstract：:Rhizomorphic lycopsids are the land plant group that includes the first giant trees to grow on Earth and extant species in the genus Isoetes. Two mutually exclusive hypotheses account for the evolution of terminal rooting axes called rootlets among the rhizomorphic lycopsids. One hypothesis states that rootlets are tr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78171-y
更新日期：2020-12-09 00:00:00
abstract：:Salivary duct carcinoma (SDC) is an uncommon, but aggressive malignant tumor with a high mortality rate. Herein, we reported the detection of somatic KRAS A146T and Q61H mutations in 2 out of 4 (50%) sarcomatoid SDC variants. Transgenic mice carrying the human oncogenic KRAS(G12V), which spatiotemporal activation by t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13347
更新日期：2015-08-20 00:00:00
abstract：:Lack of standardization and unblinding threaten the research of mechanisms involved in expectancy effects on pain. We evaluated a computer-controlled virtual experimenter (VEx) to avoid these issues. Fifty-four subjects underwent a baseline-retest heat pain protocol. Between sessions, they received an expectancy manip...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77453-9
更新日期：2020-11-23 00:00:00
abstract：:Loss of cardiomyocyte proliferative capacity after birth is a major obstacle for therapeutic heart regeneration in adult mammals. We and others have recently shown the importance of hypoxic in utero environments for active foetal cardiomyocyte proliferation. Here, we report the unexpected expression of novex-3, the sh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30886-9
更新日期：2018-08-17 00:00:00
abstract：:Although cooperation is central to the organisation of many social systems, relatively little is known about cooperation in situations of collective emergency. When groups of people flee from a danger such as a burning building or a terrorist attack, the collective benefit of cooperation is important, but the cost of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33417
更新日期：2016-09-15 00:00:00
abstract：:This study aimed to investigate the possible association between diabetes susceptibility gene transcription factor 7-like 2 (TCF7L2) and gestational diabetes mellitus (GDM) in a Chinese Han population. A total of 556 GDM patients and 500 Non-GDM were included. Eighteen single nucleotide polymorphisms (SNPs) were evalu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30686
更新日期：2016-07-28 00:00:00
abstract：:Quantum cryptography is set to become a key technology for future secure communications. However, to get maximum benefit in communication networks, transmission links will need to be shared among several quantum keys for several independent users. Such links will enable switching in quantum network nodes of the quantu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12309-3
更新日期：2017-09-29 00:00:00
abstract：:Adaptive evolution by serial subcultivation of co-cultured Bacillus thuringiensis and Ketogulonicigenium vulgare significantly enhanced the productivity of 2-keto-L-gulonic acid in two-step vitamin C production. The adaptation mechanism in K. vulgare-B. thuringiensis consortium was investigated in this study based on ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46759
更新日期：2017-04-25 00:00:00
abstract：:Neuroinflammation in utero may result in life-long neurological disabilities. Microglia play a pivotal role, but the mechanisms are poorly understood. No early postnatal treatment strategies exist to enhance neuroprotective potential of microglia. We hypothesized that agonism on α7 nicotinic acetylcholine receptor (α7...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09439-z
更新日期：2017-09-06 00:00:00
abstract：:Tin selenide has attracted much research interest due to its unprecedentedly high thermoelectric figure of merit (ZT). For real applications, it is desirable to increase the ZT value in the lower-temperature range, as the peak ZT value currently exists near the melting point. It is shown in this paper that the structu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26193
更新日期：2016-05-19 00:00:00
abstract：:Third permanent molars (M3s) are the last tooth to form but have not been used to estimate age at dental maturation in early fossil hominins because direct histological evidence for the timing of their growth has been lacking. We investigated an isolated maxillary M3 (SK 835) from the 1.5 to 1.8-million-year-old (Mya)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76032-2
更新日期：2020-11-04 00:00:00
abstract：:Photoactivated localization microscopy (PALM) is a super-resolution imaging technique based on the detection and subsequent localization of single fluorescent molecules. PALM is therefore a powerful tool in resolving structures and putative interactions of biomolecules at the ultimate analytical detection limit. Howev...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13532
更新日期：2015-09-01 00:00:00
abstract：:One mechanism of action for the anticaries effect of topical fluoridation is through precipitation of CaF2. In this in vitro study energy-dispersive x-ray spectroscopy (EDX) is used as a semiquantitative method to detect enamel fluoride-precipitation under the influence of acidic and neutral pH-value and absence or pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49742-5
更新日期：2019-09-17 00:00:00
abstract：:This study aimed to examine hemodynamic responses and the necessity of breaks under long working hours. Thirty-eight healthy males conducted PC-based work from 9:10 to 22:00. Nine 10-minute short breaks and two long breaks (a 1-hour break and a 50-minute break) were provided, and hemodynamic responses were measured re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32908-y
更新日期：2018-09-28 00:00:00
abstract：:Liver repopulation by transplanted hepatocytes has not been achieved previously in a normal liver microenvironment. Here we report that adult rat hepatocytes transduced ex vivo with a lentivirus expressing a human YapERT2 fusion protein (hYapERT2) under control of the hepatocyte-specific transthyretin (TTR) promoter r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19275
更新日期：2016-01-14 00:00:00
abstract：:Transmembrane receptor clustering is a ubiquitous phenomenon in pro- and eukaryotic cells to physically sense receptor/ligand interactions and subsequently translate an exogenous signal into a cellular response. Despite that receptor cluster formation has been described for a wide variety of receptors, ranging from ch...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28940
更新日期：2016-06-29 00:00:00
abstract：:Liposomes or biological vesicles can be created from cholesterol, phospholipid, and water. Their stability is affected by their phospholipid composition which can influence disease treatment and drug delivery efficacy. In this study, the effect of phospholipid type on the formation and stability of liposomes using coa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58730-z
更新日期：2020-02-04 00:00:00
abstract：:The corneal endothelium is essential for maintaining corneal transparency; therefore, corneal endothelial dysfunction causes serious vision loss. Tissue engineering-based therapy is potentially a less invasive and more effective therapeutic modality. We recently started a first-in-man clinical trial of cell-based ther...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15005
更新日期：2015-10-07 00:00:00
