Abstract：
:The corneal endothelium is essential for maintaining corneal transparency; therefore, corneal endothelial dysfunction causes serious vision loss. Tissue engineering-based therapy is potentially a less invasive and more effective therapeutic modality. We recently started a first-in-man clinical trial of cell-based therapy for treating corneal endothelial dysfunction in Japan. However, the senescence of corneal endothelial cells (CECs) during the serial passage culture needed to obtain massive quantities of cells for clinical use is a serious technical obstacle preventing the push of this regenerative therapy to clinical settings. Here, we show evidence from an animal model confirming that senescent cells are less effective in cell therapy. In addition, we propose that density-gradient centrifugation can eliminate the senescent cells and purify high potency CECs for clinical use. This simple technique might be applicable for other types of cells in the settings of regenerative medicine.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Okumura N,Kusakabe A,Hirano H,Inoue R,Okazaki Y,Nakano S,Kinoshita S,Koizumi Ndoi
10.1038/srep15005subject
Has Abstractpub_date
2015-10-07 00:00:00pages
15005issn
2045-2322pii
srep15005journal_volume
5pub_type
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