Integrative quantitative-phase and airy light-sheet imaging.

Abstract:

:Light-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Subedi NR,Jung PS,Bredeweg EL,Nemati S,Baker SE,Christodoulides DN,Vasdekis AE

doi

10.1038/s41598-020-76730-x

subject

Has Abstract

pub_date

2020-11-19 00:00:00

pages

20150

issue

1

issn

2045-2322

pii

10.1038/s41598-020-76730-x

journal_volume

10

pub_type

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