Abstract:
:Light-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Subedi NR,Jung PS,Bredeweg EL,Nemati S,Baker SE,Christodoulides DN,Vasdekis AEdoi
10.1038/s41598-020-76730-xsubject
Has Abstractpub_date
2020-11-19 00:00:00pages
20150issue
1issn
2045-2322pii
10.1038/s41598-020-76730-xjournal_volume
10pub_type
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journal_title:Scientific reports
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pub_type: 杂志文章
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pub_type: 杂志文章
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