Abstract：
:Keratinocytes, in response to irritants, secrete pro-inflammatory mediators which recruit and activate immune and mesenchymal cells, including fibroblasts, to repair the skin. Fibroblasts respond by synthesising collagen and promoting the crosslinking extracellular matrix (ECM). We recently showed that the deletion of Rac1 in keratinocytes causes heightened inflammation due to aberrant crosstalk with immune cells. Indeed, the skin of these mice shows a higher inflammatory response to the induction of irritant contact dermatitis (ICD), and also even to treatment with a vehicle alone, compared with controls. As inflammation is intimately linked with fibrotic disease in the skin, this raised the question as to whether this deletion may also affect the deposition and arrangement of the dermal ECM. This study assessed the effects of Rac1 deletion in keratinocytes and of the heightened inflammatory status by induction of ICD on the tissue localisation and arrangements of dermal collagen. Qualitative analysis did not reveal evidence for the formation of pathologies in the dermis. However, quantitative analysis did reveal some perturbations in the dermal matrix, namely that only the combination of the lack of Rac1 and ICD affects the architectural organisation of the dermal collagen, and that a higher inflammatory state in the tissue (i.e. when Rac1 is deleted in the keratinocytes or ICD is induced in the skin, or a combination of both) influences the diameter of the collagen fibrils. It is proposed that this increase in the diameter of collagen fibrils due to inflammation may serve as pre-fibrotic marker enabling earlier determination of fibrosis and earlier treatment. This study has revealed previously unknown effects on the ECM due to the deletion of Rac1 in keratinocytes.
journal_name
J Anatjournal_title
Journal of anatomyauthors
Stanley A,Pedersen E,Brakebusch C,Quondamatteo Fdoi
10.1111/joa.12442subject
Has Abstractpub_date
2016-05-01 00:00:00pages
826-37issue
5eissn
0021-8782issn
1469-7580journal_volume
228pub_type
杂志文章abstract：:The spatiotemporal distribution of macrophages in the uterine wall of mice in the second half of pregnancy and the early postpartum period has been studied. Macrophages were identified using a combination of morphological criteria, the capacity to endocytose horseradish peroxidase and the expression of the Mac-1 antig...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:To evaluate whether the lines occasionally detected on clinical magnetic resonance (MR) images are genuine hippocampal layers, a formalin fixed hippocampal specimen was scanned using T2 weighted sequences at 7 Tesla (voxel dimensions 0.064 x 0.064 x 1 mm) and at 1.5 Tesla (voxel dimensions: 0.156 x 0.156 x 1 mm) and c...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.1999.19510131.x
更新日期：1999-07-01 00:00:00
abstract：:Substantial changes in bone histology accompany the secondary adaptation to life in the water. This transition is well documented in several lineages of mammals and non-avian reptiles, but has received relatively little attention in birds. This study presents new observations on the long bone microstructure of penguin...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12367
更新日期：2015-11-01 00:00:00
abstract：:Alx4 and Msx2 encode homeodomain-containing transcription factors that show a clear functional overlap. In both mice and humans, loss of function of either gene is associated with ossification defects of the skull vault, although the major effect is on the frontal bones in mice and the parietal bones in humans. This s...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.0021-8782.2004.00304.x
更新日期：2004-06-01 00:00:00
abstract：:Comb and wattle tissues, which consist of layers of epidermis, dermis and central connective tissue, are known to contain sulphated glycosaminoglycans (GAGs) including dermatan sulphate and chondroitin sulphate-dermatan sulphate copolymers. Little is known about distribution of these CAGs in each tissue. The objective...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:The aim of the study was to examine by means of stereology the effects of pinealectomy and melatonin on the structure of the adrenal cortex of ovariectomised rats and to check whether the effect of oestradiol on the rat adrenal cortex is modified by the pineal gland. Neither pinealectomy (73 days) nor melatonin treatm...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:The sacroiliac joint (SIJ) is responsible for weight transmission between the spine and lower extremity. However, details of the structure and function of the SIJ remain unclear. In a previous study, we devised a method of quantitatively evaluating the level of degeneration of the SIJ using an age estimation procedure...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12765
更新日期：2018-02-01 00:00:00
abstract：:The kinematics of swings at joints can be described fully by means of two simple ratios and the angle at which the total operative force vector pulls upon a bone. The ratios can be applied to individual muscles, thus linking structural and functional myology in an exact way. The 'spurt' and 'shunt' types of muscle are...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:The striated muscle component of the opossum oesophagus has been studied for fibre type as revealed by histochemical stains for succinic acid dehydrogenase, adenosine triphosphatase, nicotinamide adenine dinucleotide dehydrogenase and after staining with the periodic acid-Schiff reagent. The reactions were compared to...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
abstract：:The translocation of mitochondria towards the primitive inner segment of the cones in the tree shrew Tupaia belangeri was investigated by transmission electron microscopy. Throughout ontogeny the migrating mitochondria were codistributed with cytoplasmic microtubules which were preserved after the application of conve...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:This pre-embedding electron-immunocytochemical study investigated the localisation of endothelial (type III) and neuronal (type I) isoforms of nitric oxide synthase, substance P, endothelin-1 and 5-hydroxytryptamine in the human middle cerebral artery taken up to 40 h postmortem. To ¿recover' from the anoxic period so...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:This study was done in order to investigate the normal ultrastructure of well-preserved mouse spinal canal ependyma using light, scanning and transmission electron microscopy. The ependymal lining was found to consist of a simple, cuboidal epithelium essentially similar to the unspecialized cuboidal ependyma of the br...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:During ageing, a progressive loss of skeletal muscle mass and a decrease in muscle strength and endurance take place, in the condition termed sarcopenia. The mechanisms of sarcopenia are complex and still unclear; however, it is known that muscle atrophy is associated with a decline in the number and/or efficiency of ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12429
更新日期：2016-05-01 00:00:00
abstract：:A total histological grade does not necessarily distinguish between different manifestations of cartilage damage or degeneration. An accurate and reliable histological assessment method is required to separate normal and pathological tissue within a joint during treatment of degenerative joint conditions and to sub-cl...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2012.01513.x
更新日期：2012-07-01 00:00:00
abstract：:Signalling interactions between the polarizing region, which produces SHH, and the apical ectodermal ridge, which produces FGFs, are essential for outgrowth and patterning of vertebrate limbs. However, mechanisms that mediate translation of early positional information of cells into anatomy remain largely unknown. In ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2003.00134.x
更新日期：2003-01-01 00:00:00
abstract：:Maternal intraperitoneal administration of 1 mg/kg 5-azacytidine to pregnant S1c:ICR mice on gestational Day 7.5 resulted in a high incidence of exencephalic offspring. Histological examination of untreated 7.5 days mouse embryos revealed that the head folds were formed in the anterior halves of the embryos, whereas t...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:The anatomy of the hypothalamus includes many nuclei and a complex network of neurocircuits. In this context, some hypothalamic nuclei reside closer to the blood-brain barrier, allowing communication with the peripheral organs through some molecules, such as leptin. Leptin is considered the main adipokine for energy h...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.13342
更新日期：2020-10-22 00:00:00
abstract：:This study describes the degenerative changes of the axon terminals making synaptic contacts with the neurons in the submucous ganglia of guinea pig small and large intestines following left or right cervical vagotomy. There were no noticeable ultrastructural changes 1 d after the operation. Beginning at the 3rd posto...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Genetic mutation leads to anatomical variation only indirectly because many proteins involved in generating anatomical structures in embryos operate cooperatively within molecular networks. These include gene-regulatory or control networks (CNs) for timing, signaling and patterning together with the process networks (...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12205
更新日期：2014-08-01 00:00:00
abstract：:Advances in X-ray computed tomography (CT) have led to a rise in the use of non-destructive imaging methods in comparative anatomy. Among these is contrast-enhanced CT scanning, which employs chemical stains to visualize soft tissues. Specimens may then be 'digitally dissected', producing detailed, three-dimensional d...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.13007
更新日期：2019-08-01 00:00:00
abstract：:At the tip of the lower beak of the chicken there were found 15-20 specialised dermal papillae containing large numbers of mechanoreceptors. The Merkel or Grandry corpuscles were situated distally in the papillae and at the papillae base was a collection of Herbst corpuscles. The apex of the papillae, under the scanni...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:Complex I is one of the respiratory chain enzymes related to NADH dehydrogenase and is an encoded gene product derived from both nuclear and mitochondrial genomes. Transcription levels of ND1 (mitochondrial) and 51 kDa (nuclear) subunits of complex I in the postnatal development of the intrinsic muscle in rat tongues ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2003.00152.x
更新日期：2003-02-01 00:00:00
abstract：:Histochemical fibre types classified in sections stained for succinic dehydrogenase (sdh) and myosin ATPase at pH 9.4, were found to be distributed in a consistent manner within the extensor digitorum longus (EDL) and 4 soleus muscles of the adult rat. Simple morphometric techniques applied to complete transverse sect...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
abstract：:The neuromuscular junction (NMJ) is the highly specialised peripheral synapse formed between lower motor neuron terminals and muscle fibres. Post-synaptic acetylcholine receptors (AChRs), which are found in high density in the muscle membrane, bind to acetylcholine released into the synaptic cleft of the NMJ, thereby ...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.13228
更新日期：2020-10-01 00:00:00
abstract：:Although the interspecific scaling of tetrapods is well understood, remarkably little work has been done on the ontogenetic scaling within tetrapod species, whether fossil or recent. Here the ontogenetic allometry of the femur, humerus, and tibia was determined for 23 species of non-avian dinosaur by regressing log-tr...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/j.1469-7580.2010.01253.x
更新日期：2010-08-01 00:00:00
abstract：:Crocodylians occupy a key phylogenetic position for investigations of archosaur locomotor evolution. Compared to the well-studied hindlimb, relatively little is known about the skeletal movements and mechanics of the forelimb. In this study, we employed manual markerless XROMM (X-ray Reconstruction Of Moving Morpholog...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1111/joa.12102
更新日期：2013-11-01 00:00:00
abstract：:The dogma that cutaneous wound healing is impaired as a function of age is largely unsubstantiated. This can be attributed to poor experimental design of human studies, the lack of subject characterisation with the exclusion of disease processes, and the study of inappropriate animal models. Structural and functional ...
journal_title：Journal of anatomy
pub_type： 杂志文章,评审
doi：
更新日期：1995-08-01 00:00:00
abstract：:The lacrimal sac and nasolacrimal duct are surrounded by a wide cavernous system of veins and arteries comparable to a cavernous body. The present study aimed to demonstrate the ultrastructure of the nervous tissue and the localisation of neuropeptides involved in the innervation of the cavernous body, a topic not pre...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：10.1046/j.1469-7580.2000.19720177.x
更新日期：2000-08-01 00:00:00
abstract：:The present report describes quantitative studies of mitochondria from stratified squamous epithelial cells from hamster cheek pouch mucosa in differentiating compartments. Mucosa was processed for electron microscopy and, using strict sampling procedures, electron micrographs of epithelium from defined basal, spinous...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1983-06-01 00:00:00
abstract：:This study reports the tissue distribution of Hepatocyte Growth Factor-Scatter Factor (HGF/SF) in human fetal tissue using Northern analysis and in situ hybridisation techniques. In tissue from fetuses of 9-17 wk gestational age, the 6 kb mRNA transcript for HGF/SF was demonstrated in many tissues but prominently in l...
journal_title：Journal of anatomy
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
