Abstract：
:Chemical conjugation is commonly used to enhance the pharmacokinetics, biodistribution, and potency of protein therapeutics, but often leads to non-specific modification or loss of bioactivity. Here, we present a simple, versatile and widely applicable method that allows exquisite N-terminal specific modification of proteins. Combining reversible side-chain blocking and protease mediated cleavage of a commonly used HIS tag appended to a protein, we generate with high yield and purity exquisitely site specific and selective bio-conjugates of TNF-α by using amine reactive NHS ester chemistry. We confirm the N terminal selectivity and specificity using mass spectral analyses and show near complete retention of the biological activity of our model protein both in vitro and in vivo murine models. We believe that this methodology would be applicable to a variety of potentially therapeutic proteins and the specificity afforded by this technique would allow for rapid generation of novel biologics.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sur S,Qiao Y,Fries A,O'Meally RN,Cole RN,Kinzler KW,Vogelstein B,Zhou Sdoi
10.1038/srep18363subject
Has Abstractpub_date
2015-12-17 00:00:00pages
18363issn
2045-2322pii
srep18363journal_volume
5pub_type
杂志文章abstract：:Plasmonics is a rapidly emerging platform for quantum state engineering with the potential for building ultra-compact and hybrid optoelectronic devices. Recent experiments have shown that despite the presence of decoherence and loss, photon statistics and entanglement can be preserved in single plasmonic systems. This...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18313
更新日期：2015-12-16 00:00:00
abstract：:Since the advent of graphene, a variety of studies have been performed to elucidate its fundamental physics, or to explore its practical applications. Gate-tunable resistance is one of the most important properties of graphene and has been studied in 1-3 layer graphene in a number of efforts to control the band gap to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32214-7
更新日期：2018-09-18 00:00:00
abstract：:Studies investigating association of depression with overall survival (OS) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) yielded conflicting results. A nationwide cohort study, which included all adult patients [n = 7,170; depression group, 13.3% (N = 956); non-depression group, 86.7% (N = 6,214...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71208-2
更新日期：2020-09-17 00:00:00
abstract：:The Sigma-1 receptor (S1R) is known to play a protective role in the central nervous system including the retina. A major barrier for understanding the underlying mechanism is an ambiguity of S1R subcellular localizations. We thus conducted the first electron microscopy (EM) study of S1R subcellular distribution in th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10689
更新日期：2015-06-02 00:00:00
abstract：:Two important theories in spatial modelling relate to structural and spatial dependence. Structural dependence refers to environmental state-factor models, where an environmental property is modelled as a function of the states and interactions of environmental predictors, such as climate, parent material or relief. C...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73773-y
更新日期：2020-10-07 00:00:00
abstract：:The health effects of the endocrine disruptor Bisphenol A (BPA) led to its partial replacement with Bisphenol S (BPS) in several products including food containers, toys, and thermal paper receipts. The acute effects of BPS on myocardial contractility are unknown. We perfused mouse hearts from both sexes for 15 min wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52350-y
更新日期：2019-11-04 00:00:00
abstract：:Increasing human activity along the coast has amplified the extinction risk of inshore delphinids. Informed selection and prioritisation of areas for the conservation of inshore delphinids requires a comprehensive understanding of their distribution and habitat use. In this study, we applied an ensemble species distri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69863-6
更新日期：2020-09-01 00:00:00
abstract：:Glutamate (Glu), the key excitatory neurotransmitter in the central nervous system, is considered essential for brain functioning and has a vital role in learning and memory formation. Earlier it was considered as a harmful agent but later found to be useful for many body functions. However, studies regarding the effe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68041-y
更新日期：2020-07-08 00:00:00
abstract：:There is an urgent need for sensors deployed during focal therapies to inform treatment planning and in vivo monitoring in thin tissues. Specifically, the measurement of thermal properties, cooling surface contact, tissue thickness, blood flow and phase change with mm to sub mm accuracy are needed. As a proof of princ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21395
更新日期：2016-02-26 00:00:00
abstract：:Gait is an important health indicator and poor gait is strongly associated with disability and risk of falls. Thyroid dysfunction is suggested as a potential determinant of gait deterioration, but this has not been explored in a population-based study. We therefore investigated the association of thyroid function with...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep38912
更新日期：2016-12-14 00:00:00
abstract：:The use of impedance-based label free cell analysis is increasingly popular and has many different applications. Here, we report that a real-time cell analyzer (RTCA) can be used to study the stimulation of Natural Killer (NK) cells. Engagement of NK cells via plate-bound antibodies directed against different activati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23368-5
更新日期：2018-03-21 00:00:00
abstract：:Neutrophils are the first immune cells to kill invading microbes at sites of infection using a variety of processes, including the release of proteases, phagocytosis and the production of neutrophil extracellular traps (NETs). NET formation, or NETosis, is a specific and highly efficient process, which is induced by a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53058-9
更新日期：2019-11-12 00:00:00
abstract：:Elucidating arbuscular mycorrhizal (AM) fungal responses to elevation changes is critical to improve understanding of microbial function in ecosystems under global asymmetrical climate change scenarios. Here we examined AM fungal community in a two-year reciprocal translocation of vegetation-intact soil blocks along a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36606
更新日期：2016-11-09 00:00:00
abstract：:We demonstrate that laser peening coupled with sintering of CdTe nanowire films substantially enhances film quality and charge transfer while largely maintaining basic particle morphology. During the laser peening phase, a shockwave is used to compress the film. Laser sintering comprises the second step, where a nanos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16052
更新日期：2015-11-03 00:00:00
abstract：:Recent research has shown that the cerebellum is involved not only in motor control but also in higher-level activities, which are closely related to creativity. This study aimed to explore the role of the cerebellum in visual divergent thinking based on its intrinsic activity. To this end, we selected the resting-sta...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73679-9
更新日期：2020-10-06 00:00:00
abstract：:Croup is the leading infectious disease resulting in pediatric upper airway obstruction. Our purpose is to analyze diverse features of neck radiographs could be seen as an objective tool to predict outcomes in patients with croup. One hundred and ninety-two patients were prospectively recruited in pediatric emergency ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54140-y
更新日期：2019-11-29 00:00:00
abstract：:Avian influenza viruses (AIV) are classified into 16 hemagglutinin (HA; H1-H16) and 9 neuraminidase (NA; N1-N9) subtypes. All AIV are low pathogenic (LP) in birds, but subtypes H5 and H7 AIV can evolve into highly pathogenic (HP) forms. In the last two decades evolution of HPAIV H7 from LPAIV has been frequently repor...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47938-3
更新日期：2019-08-09 00:00:00
abstract：:Cyclamen persicum (cyclamen) is a commercially valuable, winter-blooming perennial plant. We cloned two cyclamen orthologues of AGAMOUS (AG), CpAG1 and CpAG2, which are mainly expressed in the stamen and carpel, respectively. Cyclamen flowers have 5 petals, but expression of a chimeric repressor of CpAG1 (CpAG1-SRDX) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02641
更新日期：2013-01-01 00:00:00
abstract：:Keloid is the abnormal wound healing puzzled by the aggressive growth and high recurrence rate due to its unrevealed key pathogenic mechanism. MicroRNAs contribute to a series of biological processes including epithelial-mesenchymal transition (EMT) and cells stemness involved in fibrotic disease. Here, using microRNA...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28281
更新日期：2016-09-06 00:00:00
abstract：:If a cultural feature is transmitted over generations and exposed to stochastic selection when spreading in a population, its evolution may be governed by statistical laws and be partly predictable, as in the case of genetic evolution. Music exhibits steady changes of styles over time, with new characteristics develop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52380-6
更新日期：2019-11-05 00:00:00
abstract：:Cas13 endonuclease activity depends on the RNA local secondary structure with strong preference for single-stranded (SS) regions. Hence, it becomes indispensable to identify the SS regions for effective Cas13 mediated RNA knockdown. We herein present rational gRNA design by integrating experimental structure-seq data ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68459-4
更新日期：2020-07-14 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50670-7
更新日期：2019-10-09 00:00:00
abstract：:Congenital cataract is the most frequent inherited ocular disorder and the most leading cause of lifelong visual loss. The screening of pathogenic mutations can be very challenging in some cases, for congenital cataracts are clinically and genetically heterogeneous diseases. The aim of this study is to investigate the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-01182-9
更新日期：2017-04-27 00:00:00
abstract：:This study examined the performance and photo-bias stability of double-channel ZnSnO/InZnO (ZTO/IZO) thin-film transistors. The field-effect mobility (μFE) and photo-bias stability of the double-channel device were improved by increasing the thickness of the front IZO film (t(int)) compared to the single-ZTO-channel d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03765
更新日期：2014-01-20 00:00:00
abstract：:Designing a new generation of energy-intensive and sustainable electrode materials for batteries to power a variety of applications is an imperative task. The use of biomaterials as a nanosized structural template for these materials has the potential to produce hitherto unachievable structures. In this report, we hav...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07736
更新日期：2015-01-13 00:00:00
abstract：:Liver failure, whether arising directly from acute liver failure or from decompensated chronic liver disease is an increasing problem worldwide and results in many deaths. In the UK only 10% of individuals requiring a liver transplant receive one. Thus the need for alternative treatments is paramount. A BioArtificial ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15021-4
更新日期：2017-11-06 00:00:00
abstract：:Dengue virus infection is a global threat for which no specific treatment has not been established. Previous reports suggested chrysin and flavanone derivatives were potential flaviviral inhibitors. Here, we reported two halogenated chrysins, abbreviated FV13 and FV14, were highly potent against DENV1-4 and ZIKV infec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14121-5
更新日期：2017-10-20 00:00:00
abstract：:A peptide vaccine designed to induce T-cell immunity to telomerase, GV1001, has been shown to modulate cellular signaling pathways and confer a direct anti-cancer effect through the interaction with heat shock protein (HSP) 90 and 70. Here, we have found that GV1001 can modulate transactivation protein-mediated human ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28896
更新日期：2016-07-01 00:00:00
abstract：:A novel type of transparent monitor with high-resolution images based on Si-SiO2 core-shell nanoparticles is presented in this contribution. In this monitor, a quasi-array of nanoparticles was used to obtain a very sharp scattering profile. For this purpose, the Si-SiO2 nanoparticles were synthesized and with controll...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38771-9
更新日期：2019-02-19 00:00:00
abstract：:Phosphorylation plays important roles in several processes including synaptic plasticity and memory. The critical role of extracellular signal-regulated kinase (ERK) in these processes is well established. ERK is activated in a sustained manner by different stimuli. However, the mechanisms of sustained ERK activation ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01103
更新日期：2013-01-01 00:00:00
