Abstract：
:Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells. CXCR4 is a co-receptor for the human immunodeficiency virus type 1 (HIV-1) infection and has been considered as an important therapeutic target for AIDS. CXCR4 mediates viral entry into human CD4(+) cells by binding to envelope protein, gp120. Here, we show that human CXCR4 gene is efficiently disrupted by CRISPR/Cas9-mediated genome editing, leading to HIV-1 resistance of human primary CD4(+) T cells. We also show that the Cas9-mediated ablation of CXCR4 demonstrated high specificity and negligible off-target effects without affecting cell division and propagation. The precise and efficient genome editing of CXCR4 will provide a new strategy for therapeutic application against HIV-1 infection.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hou P,Chen S,Wang S,Yu X,Chen Y,Jiang M,Zhuang K,Ho W,Hou W,Huang J,Guo Ddoi
10.1038/srep15577subject
Has Abstractpub_date
2015-10-20 00:00:00pages
15577issn
2045-2322pii
srep15577journal_volume
5pub_type
杂志文章abstract：:The aims of the study were to clarify the impact of storage time and centrifugation strategy on brain-derived neurotrophic factor (BDNF) levels in human serum and plasma samples. In addition, we analyzed associations between BDNF levels, cardiorespiratory fitness and waist circumference. Seventeen healthy males (25.2 ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45976-5
更新日期：2019-07-04 00:00:00
abstract：:Urinary tract infections (UTIs) are the third most common types of infection in human medicine worldwide. There is increasing appreciation for the pathogenic role of Gram-positive cocci (GPC) in UTIs, as they have a plethora of virulence factors, maintaining their pathogenicity and high affinity for the epithelial cel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74834-y
更新日期：2020-10-19 00:00:00
abstract：:Currently, a comprehensive assessment between mitochondrial DNA (mtDNA) content and cancer risk is lacking. We designed this meta-analysis to test the hypothesis that altered mtDNA copy number might influence genetic susceptibility to some specific types of cancer. The processes of literature search, eligibility appra...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep10039
更新日期：2015-05-08 00:00:00
abstract：:Whole-room indirect calorimeters (WRICs) have traditionally been used for real-time resting metabolic rate (RMR) measurements, while metabolic rate (MR) during short-interval exercises has commonly been measured by metabolic carts (MCs). This study aims to investigate the feasibility of incorporating short-interval ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71001-1
更新日期：2020-08-31 00:00:00
abstract：:In this study, we reported a new technique based on laser speckle flowgraphy to record the ocular blood flow in rabbits under deep anesthesia, and proposed parameters to characterize retinal ischemia. We applied the proposed technique to study the correlation of blood flow between the eyes of normal non-anesthetized a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24141-4
更新日期：2018-04-09 00:00:00
abstract：:This paper has been retracted at the request of the authors. ...
journal_title：Scientific reports
pub_type： 撤回出版物
doi：10.1038/s41598-018-29892-8
更新日期：2018-08-02 00:00:00
abstract：:Autism spectrum disorder (ASD) is marked by atypical trajectory of brain maturation, yet the developmental abnormalities in brain function remain unclear. The current study examined the effect of age on amplitude of low-frequency fluctuations (ALFF) in ASD and typical controls (TC) using a cross-sectional design. We c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39822
更新日期：2017-01-06 00:00:00
abstract：:Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11835
更新日期：2015-07-02 00:00:00
abstract：:Knockin (KI) mouse carrying a point mutation has been an invaluable tool for disease modeling and analysis. Genome editing technologies using the CRISPR/Cas system has emerged as an alternative way to create KI mice. However, if the mice carry nucleotide insertions and/or deletions (InDels) in other genes, which could...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34703
更新日期：2016-10-04 00:00:00
abstract：:RNA is transcribed from DNA, and therefore, there should be no RNA transcript from the deleted DNA region. Our study attempted to analyse whether any RNA cache that maps the deleted regions is present in human cells. Using data from the 1000 genome project, we selected 41 CEPH (CEU) and 38 Yoruba (YRI) samples that in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37452
更新日期：2016-11-17 00:00:00
abstract：:By using a half soap bubble heated from below, we obtain large isolated single vortices whose properties as well as their intensity are measured under different conditions. By studying the effects of rotation of the bubble on the vortex properties, we found that rotation favors vortices near the pole. Rotation also in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03455
更新日期：2013-12-13 00:00:00
abstract：:Chronic atrophic gastritis (CAG) is a common gastrointestinal disease which has been considered as precancerous lesions of gastric carcinoma. Previously, electro-acupuncture stimulation has been shown to be effective in ameliorating symptoms of CAG. However the underlying mechanism of this beneficial treatment is yet ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45580
更新日期：2017-03-30 00:00:00
abstract：:The 'microgenderome' provides a paradigm shift that highlights the role of sex differences in the host-microbiota interaction relevant for autoimmune and neuro-immune conditions. Analysis of cross-sectional self-report and faecal microbial data from 274 patients with Myalgic Encephalomyelitis/Chronic Fatigue Syndrome ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19171
更新日期：2016-01-13 00:00:00
abstract：:Conifer wood is an exceptionally stiff and strong material when its cellulose microfibrils are well aligned. However, it is not well understood how the polymer components cellulose, hemicelluloses and lignin co-operate to resist tensile stress in wood. From X-ray scattering, neutron scattering and spectroscopic data, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79676-2
更新日期：2021-01-11 00:00:00
abstract：:Single nucleotide polymorphisms (SNPs) and genes associated with susceptibility to hepatitis B virus (HBV) infection that have been identified by genome-wide association studies explain only a limited portion of the known heritability, indicating more genetic variants remain to be discovered. In this study, we adopted...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49777-8
更新日期：2019-09-17 00:00:00
abstract：:We have studied in-situ cleaved (0001) surfaces of the magnetic Weyl semimetal Mn3Sn by low-temperature scanning tunneling microscopy and spectroscopy (STM/S). It was found that freshly cleaved Mn3Sn surfaces are covered with unknown clusters, and the application of voltage pulses in the tunneling condition was needed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45958-7
更新日期：2019-07-04 00:00:00
abstract：:We report a recent experimental simulation of a controlled-NOT gate operation based on polarization correlation measurements of thermal fields in photon-number fluctuations. The interference between pairs of correlated paths at the very heart of these experiments has the potential for the simulation of correlations be...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30152
更新日期：2016-07-21 00:00:00
abstract：:Thymocyte and T cell trafficking relies on signals initiated by G-protein coupled receptors. To address the importance of the G-proteins Gαi2 and Gαi3 in thymocyte and T cell function, we developed several mouse models. Gαi2 deficiency in hematopoietic progenitors led to a small thymus, a double negative (DN)1/DN2 thy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04537-4
更新日期：2017-06-23 00:00:00
abstract：:We demonstrate theoretically that photon-photon attraction can be engineered in the continuum of scattering states for pairs of photons propagating in a hollow-core photonic crystal fiber filled with cold atoms. The atoms are regularly spaced in an optical lattice configuration and the photons are resonantly tuned to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25630
更新日期：2016-05-12 00:00:00
abstract：:High-throughput screening facilities do not generally support biosafety level 3 organisms such as Mycobacterium tuberculosis. To discover not only antibacterials, but also virulence inhibitors with either bacterial or host cell targets, an assay monitoring lung fibroblast survival upon infection was developed and opti...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37176-4
更新日期：2019-01-10 00:00:00
abstract：:The hypothalamic-pituitary-adrenal (HPA) axis is an important component of neuroendocrine stress regulation and coping behavior. Transcriptome profiles of the hypothalamus and adrenal gland were assessed to identify molecular pathways and candidate genes for coping behavior in pigs. Ten each of high- (HR) and low- (LR...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49521-2
更新日期：2019-09-10 00:00:00
abstract：:Lipid mediators play pivotal roles in colorectal cancer and colitis, but only a limited member of the phospholipase A2 (PLA2) subtypes, which lie upstream of various lipid mediators, have been implicated in the positive or negative regulation of these diseases. Clinical and biochemical evidence suggests that secreted ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12434-z
更新日期：2017-09-25 00:00:00
abstract：:Biology is adapted to Earth's gravity force, and the long-term effects of varying gravity on the development of animals is unclear. Previously, we reported that high gravity, called hypergravity, increases defects in the development of motor neuron axons in the nematode Caenorhabditis elegans. Here, we show that a mut...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78414-y
更新日期：2020-12-03 00:00:00
abstract：:Despite the effectiveness of combined anti-retroviral therapy, human immunodeficiency virus (HIV) infected-patients frequently report diarrhea and neuropsychological deficits. It is claimed that the viral HIV-1 Trans activating factor (HIV-1 Tat) protein is responsible for both diarrhea and neurotoxic effects, but the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05245-9
更新日期：2017-08-10 00:00:00
abstract：:Ncb2, the β subunit of NC2 complex, a heterodimeric regulator of transcription was earlier shown to be involved in the activated transcription of CDR1 gene in azole resistant isolate (AR) of Candida albicans. This study examines its genome-wide role by profiling Ncb2 occupancy between genetically matched pair of azole...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46084
更新日期：2017-04-06 00:00:00
abstract：:Myocardial infarction (MI) can result in sympathetic nerve loss in the infarct region. However, the contribution of hypo-innervation to electrophysiological remodeling, independent from MI-induced ischemia and fibrosis, has not been comprehensively investigated. We present a novel mouse model of regional cardiac sympa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75903-y
更新日期：2020-11-02 00:00:00
abstract：:Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46686
更新日期：2017-04-24 00:00:00
abstract：:Growing evidence indicates that nomogram combined with the biomarkers of systemic inflammation response could provide more accurate prediction than conventional staging systems in tumors. This study aimed to establish an effective prognostic nomogram for resectable thoracic esophageal squamous cell carcinoma (ESCC) ba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18811
更新日期：2015-12-22 00:00:00
abstract：:Five selected carbazole derivatives, namely carbazole, 3,6-dibromocarbazole, 2-hydroxycarbazole, 1,2,3,4-tetrahydrocarbazole and 9-(2-ethylhexyl)carbazole-3,6-dicarboxaldehyde were investigated for their inhibitive effects on Desulfovibrio vulgaris (D. vulgaris) induced corrosion of mild steel and in 1 M HCl medium us...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02446-0
更新日期：2017-05-26 00:00:00
abstract：:Thaumarchaeota and the gene encoding for a subunit of ammonia monooxygenase (amoA) are ubiquitous in Polar Seas, and some Thaumarchaeota also have a gene coding for ureC, diagnostic for urease. Using quantitative PCR we investigated the occurrence of genes and transcripts of ureC and amoA in Arctic samples from winter...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04661
更新日期：2014-04-11 00:00:00
