Abstract:
:Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of C-QDs into embryos and larvae by soaking and microinjection. The multicolor of C-QDs was validated with their imaging for zebrafish embryo. The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo. All of the results confirmed the high biocompatibility and low toxicity of C-QDs as imaging probe. The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution. Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs. The operations related zebrafish are suitable for the study of the toxicity, adverse effects, transport, and biocompatibility of nanomaterials as well as for drug screening with zebrafish as model.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kang YF,Li YH,Fang YW,Xu Y,Wei XM,Yin XBdoi
10.1038/srep11835subject
Has Abstractpub_date
2015-07-02 00:00:00pages
11835issn
2045-2322pii
srep11835journal_volume
5pub_type
杂志文章abstract::To assess the response of lichen elemental compositions to road traffic and species difference in the context of high dust input and anthropogenic emissions, two foliose epiphytic lichens (Phaeophyscia hirtuosa, PHh; Candelaria fibrosa, CAf) were sampled near a road adjacent to Dolon Nor Town (Duolun County, Inner Mon...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06027-z
更新日期:2017-07-17 00:00:00
abstract::One way of expediting materials development is to decrease the need for new experiments by making greater use of published literature. Here, we use data mining and automated image analysis to gather new insights on nanoporous gold (NPG) without conducting additional experiments or simulations. NPG is a three-dimension...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25122-3
更新日期:2018-04-30 00:00:00
abstract::Real-time impairment of ocular blood flow (OBF) under common carotid artery stenosis (CCAS) has not been ascertained. We aimed to longitudinally assess the impact of CCAS on OBF using a rabbit model. About 75% stenosis was created by tying the common carotid artery with a plastic mandrel using a nylon suture. The plas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72556-9
更新日期:2020-09-28 00:00:00
abstract::Dissection of the neural circuits of the cerebral cortex is essential for studying mechanisms underlying brain function. Herein, combining a retrograde rabies tracing system with fluorescent micro-optical sectional tomography, we investigated long-range input neurons of corticotropin-releasing hormone containing neuro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68115-x
更新日期:2020-07-22 00:00:00
abstract::Facial emotion perception plays a key role in interpersonal communication and is a precursor for a variety of socio-cognitive abilities. One brain region thought to support emotion perception is the inferior frontal cortex (IFC). The current study aimed to examine whether modulating neural activity in the IFC using hi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11578-2
更新日期:2017-09-12 00:00:00
abstract::Unpleasant emotional distraction can impair the retention of non-emotional information in working memory (WM). Research links the prefrontal cortex with the successful control of such biologically relevant distractors, although the temporal changes in this brain mechanism remain unexplored. We use magnetoencephalograp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10046
更新日期:2015-06-12 00:00:00
abstract::Addiction to cocaine and other psychostimulants represents a major public health crisis. The development and persistence of addictive behaviors comes from a complex interaction of genes and environment - the precise mechanisms of which remain elusive. In recent years a surge of evidence has suggested that the gut micr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35455
更新日期:2016-10-18 00:00:00
abstract::A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41598-018-20356-7
更新日期:2018-02-02 00:00:00
abstract::Kashin-Beck disease (KBD) is a chronic osteoarthropathy, which manifests as joint deformities and growth retardation. Only a few genetic studies of growth retardation associated with the KBD have been carried out by now. In this study, we conducted a two-stage bivariate genome-wide association study (BGWAS) of the KBD...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31792
更新日期:2016-08-22 00:00:00
abstract::Flavonoids and lignans are polyphenol classes with anticarcinogenic activities against colorectal cancer (CRC). However, very limited epidemiological evidence exists on their effects on CRC prognosis. This study aimed to evaluate the association between flavonoid and lignan intakes with the risk of CRC recurrence and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14148
更新日期:2015-09-15 00:00:00
abstract::Human papillomaviruses enter host cells via a clathrin-independent endocytic pathway involving tetraspanin proteins. However, post-endocytic trafficking required for virus capsid disassembly remains unclear. Here we demonstrate that the early trafficking pathway of internalised HPV particles involves tetraspanin CD63,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32337
更新日期:2016-08-31 00:00:00
abstract::Antimonite [Sb(III)]-oxidizing bacteria can transform the toxic Sb(III) into the less toxic antimonate [Sb(V)]. Recently, the cytoplasmic Sb(III)-oxidase AnoA and the periplasmic arsenite [As(III)] oxidase AioAB were shown to responsible for bacterial Sb(III) oxidation, however, disruption of each gene only partially ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43225
更新日期:2017-03-02 00:00:00
abstract::Herbal medicines usually contain a large group of chemical components, which may be transformed into more complex metabolites in vivo. In this study, we proposed a knowledge-transmitting strategy for metabolites identification of compound formulas. Gegen-Qinlian Decoction (GQD) is a classical formula in traditional Ch...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39534
更新日期:2016-12-20 00:00:00
abstract::The lack of a precisely-dated, unequivocal climate proxy from northern China, where precipitation variability is traditionally considered as an East Asian summer monsoon (EASM) indicator, impedes our understanding of the behaviour and dynamics of the EASM. Here we present a well-dated, pollen-based, ~20-yr-resolution ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11186
更新日期:2015-06-18 00:00:00
abstract::The possibility of cluster radioactivity (CR) of the neutron-deficient nuclei in the trans-tin region is explored by using the effective liquid drop model (ELDM), generalized liquid drop model (GLDM), and several sets of analytic formulas. It is found that the minimal half-lives are at Nd = 50 (Nd is the neutron numbe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65585-x
更新日期:2020-06-04 00:00:00
abstract::We demonstrate a tool for quantitative measurements in the extreme ultraviolet (EUV) spectral region measuring spatially resolved atomic ionization products at the focus of an EUV beam. The ionizing radiation is a comb of the 11(th)-15(th) harmonics of a Ti:Sapphire femtosecond laser beam produced in a Xenon gas jet. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21556
更新日期:2016-02-12 00:00:00
abstract::The CRISPR/Cas9 system is widely used to generate gene-edited animals. Here, we developed an efficient system for generating genetically modified mice using maternal Cas9 from Cas9 transgenic mice. Using this system, we achieved lower mosaicism and higher rates of knock-in success, gene-editing, and birth compared to ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57996-7
更新日期:2020-01-23 00:00:00
abstract::The porosity and utilization of platinum catalysts have a direct impact on their performance within proton exchange membrane fuel cells. It is desirable to identify methods that can prepare these catalysts with the desired features, and that can be widely implemented using existing and industrially scalable techniques...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38855-6
更新日期:2019-03-11 00:00:00
abstract::Preterm infants are susceptible to inflammation-induced white matter injury but the exposures that lead to this are uncertain. Histologic chorioamnionitis (HCA) reflects intrauterine inflammation, can trigger a fetal inflammatory response, and is closely associated with premature birth. In a cohort of 90 preterm infan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37932
更新日期:2016-12-01 00:00:00
abstract::Ultra-high resolution optical coherence tomography (UHR-OCT) can image the corneal epithelium and Bowman's layer and measurement the thicknesses. The purpose of this study was to validate the diagnostic power of vertical thickness profiles of the corneal epithelium and Bowman's layer imaged by UHR-OCT in the diagnosis...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep31550
更新日期:2016-08-11 00:00:00
abstract::In this report we have evaluated radiation effective dose received by patients during ECG-gated CCTA examinations based on gender, heart rate, tube voltage protocol and body mass index (BMI). A total of 1,824 patients were retrospectively recruited (1,139 men and 685 women) and they were divided into Group 1 (CCTA wit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40758-5
更新日期:2019-03-13 00:00:00
abstract::The molecular structure of the hole transporting material (HTM) play an important role in hole extraction in a perovskite solar cells. It has a significant influence on the molecular planarity, energy level, and charge transport properties. Understanding the relationship between the chemical structure of the HTM's and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00271-z
更新日期:2017-03-10 00:00:00
abstract::Selection may favour individuals of the same population to differ consistently in nutritional preference, for example, because optimal diets covary with morphology or personality. We provided Southern field crickets (Gryllus bimaculatus) with two synthetic food sources (carbohydrates and proteins) and quantified repea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29071
更新日期:2016-06-30 00:00:00
abstract::Cognitive aging creates major individual and societal burden, motivating search for treatment and preventive care strategies. Behavioural interventions can improve cognitive performance in older age, but effects are small. Basic research has implicated dopaminergic signalling in plasticity. We investigated whether sup...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-020-62172-y
更新日期:2020-03-23 00:00:00
abstract::The activation of the majority of AGC kinases is regulated by two phosphorylation events on two conserved serine/threonine residues located on the activation loop and on the hydrophobic motif, respectively. In AGC kinase family, phosphomimetic substitutions with aspartate or glutamate, leading to constitutive activati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56937-3
更新日期:2020-01-17 00:00:00
abstract::A challenge in regenerating large bone defects under load is to create scaffolds with large and interconnected pores while providing a compressive strength comparable to cortical bone (100-150 MPa). Here we design a novel hexagonal architecture for a glass-ceramic scaffold to fabricate an anisotropic, highly porous th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19468
更新日期:2016-01-19 00:00:00
abstract::Rhythmically bedded cherts are observed in both pelagic marine and lacustrine deposits, but the formation mechanism in the latter remains highly uncertain. Our study of alternating chert-dolomite beds in the Eocene Green River Formation, Utah, USA reveals dense accumulations of organic-matter spheres (30-50 μm diamete...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52862-7
更新日期:2019-11-11 00:00:00
abstract::Phagocytosis is a process in which target cells or particles are engulfed and taken up by other cells, typically professional phagocytes; this process is crucial in many physiological processes and disease states. The detection of targets for phagocytosis is directed by a complex repertoire of cell surface receptors. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-38127-9
更新日期:2019-02-13 00:00:00
abstract::Environmental cycles often influence the presence of animals, creating patterns at different temporal scales, which may mean that their effects overlap and/or interact. Interactions between diel and seasonal cycles have been reported to influence fish behaviour but little is known about such interactions in marine top...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38900-4
更新日期:2019-02-22 00:00:00
abstract::Epigenetic deregulation, such as the reduction of histone acetylation levels, is thought to be causally linked to various maladies associated with aging. Consequently, histone deacetylase inhibitors are suggested to serve as epigenetic therapy by increasing histone acetylation. However, previous work suggests that man...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22674-2
更新日期:2018-03-08 00:00:00