Abstract：
:Orientation controllable DNA biosensors hold great application potentials in recognizing small molecules and detecting DNA hybridization. Though electric field is usually used to control the orientation of DNA molecules, it is also of great importance and significance to seek for other triggered methods to control the DNA orientation. Here, we design a new strategy for controlling DNA orientation in biosensors. The main idea is to copolymerize DNA molecules with responsive polymers that can show swelling/deswelling transitions due to the change of external stimuli, and then graft the copolymers onto an uncharged substrate. In order to highlight the responsive characteristic, we take thermo-responsive polymers as an example, and reveal multi-responsive behavior and the underlying molecular mechanism of the DNA orientation by combining dissipative particle dynamics simulation and molecular theory. Since swelling/deswelling transitions can be also realized by using other stimuli-responsive (like pH and light) polymers, the present strategy is universal, which can enrich the methods of controlling DNA orientation and may assist with the design of the next generation of biosensors.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Feng C,Ding HM,Ren CL,Ma YQdoi
10.1038/srep14415subject
Has Abstractpub_date
2015-09-24 00:00:00pages
14415issn
2045-2322pii
srep14415journal_volume
5pub_type
杂志文章abstract：:There is a need for noninvasive repeatable biomarkers to detect early cancer treatment response and spare non-responders unnecessary morbidities and costs. Here, we introduce three-dimensional (3D) dynamic contrast enhanced ultrasound (DCE-US) perfusion map characterization as inexpensive, bedside and longitudinal ind...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63810-1
更新日期：2020-04-24 00:00:00
abstract：:To evaluate the corneal spherical aberrations in cataract patients with and without high myopia, we conducted a retrospective case series of 502 cataract eyes with high myopia and 1500 age-related cataract eyes and measure their corneal biometric data and axial length using Pentacam and IOLMaster. Both the anterior an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36539-1
更新日期：2019-02-05 00:00:00
abstract：:Dissection of the neural circuits of the cerebral cortex is essential for studying mechanisms underlying brain function. Herein, combining a retrograde rabies tracing system with fluorescent micro-optical sectional tomography, we investigated long-range input neurons of corticotropin-releasing hormone containing neuro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68115-x
更新日期：2020-07-22 00:00:00
abstract：:Understanding and manipulating the dynamic properties of the magnetic vortices stabilized in patterned ferromagnetic structures are of great interest owing to the superior resonant features with the high thermal stability and their flexible tunability. So far, numerous methods for investigating the dynamic properties ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17922
更新日期：2015-12-09 00:00:00
abstract：:We present a mechanically active cell culture substrate that produces complex strain patterns and generates extremely high strain rates. The transparent miniaturized cell stretcher is compatible with live cell microscopy and provides a very compact and portable alternative to other systems. A cell monolayer is culture...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27915-y
更新日期：2018-07-02 00:00:00
abstract：:Stable isotope mixing models are regularly used to provide probabilistic estimates of source contributions to dietary mixtures. Whilst Bayesian implementations of isotope mixing models have become prominent, the use of appropriate diet-tissue discrimination factors (DTDFs) remains as the least resolved aspect. The DTD...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73019-x
更新日期：2020-09-25 00:00:00
abstract：:Early detection of breast cancer and its correct stage determination are important for prognosis and rendering appropriate personalized clinical treatment to breast cancer patients. However, despite considerable efforts and progress, there is a need to identify the specific genomic factors responsible for, or accompan...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60740-w
更新日期：2020-03-05 00:00:00
abstract：:Determining oil migration distances from source rocks to reservoirs can greatly help in the search for new petroleum accumulations. Concentrations and ratios of polar organic compounds are known to change due to preferential sorption of these compounds in migrating oils onto immobile mineral surfaces. However, these c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02487
更新日期：2013-01-01 00:00:00
abstract：:Doping with the optimum concentration of carriers (electrons or holes) can modify the physical properties of materials. Therefore, improved ways to achieve carrier doping have been pursued extensively for more than 50 years. Metal-intercalation is one of the most important techniques for electron doping of organic / i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18931
更新日期：2016-01-06 00:00:00
abstract：:The interaction between the T cell antigen receptor (TCR) and antigenic peptide in complex with major histocompatibility complex (MHC) molecules is a crucial step in T cell activation. The relative contributions of TCR:peptide and TCR:MHC contacts to the overall binding energy remain unclear. This has important implic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35326
更新日期：2016-10-13 00:00:00
abstract：:Drug-resistant bacteria represent a significant global threat. Given the dearth of new antibiotics, host-directed therapies (HDTs) are especially desirable. As IFN-gamma (IFNγ) plays a central role in host resistance to intracellular bacteria, including Mycobacterium tuberculosis, we searched for small molecules to au...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24409
更新日期：2016-04-18 00:00:00
abstract：:We propose a novel injection scheme for laser-driven wakefield acceleration in which controllable localized electron injection is obtained by inserting nanoparticles into a plasma medium. The nanoparticles provide a very confined electric field that triggers localized electron injection where nonlinear plasma waves ar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34998-0
更新日期：2018-11-16 00:00:00
abstract：:To examine associations between cognitive function and ophthalmological parameters, the population-based Beijing Eye Study examined ophthalmologically and physically 3127 individuals (mean age: 64.2 ± 9.8 years). Using the mini-mental state examination, cognitive function was assessed as cognitive function score (CFS)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23314-5
更新日期：2018-03-19 00:00:00
abstract：:Guppy (Poecilia reticulata) is an ideal model for studying environmental estrogens, and its large caudal fin has a high capacity to regenerate. This study analyzed the feasibility of caudal fin for detecting vitellogenin (Vtg), the most commonly used biomarker of environmental estrogens. Firstly, a sandwich ELISA for ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06670-6
更新日期：2017-08-09 00:00:00
abstract：:We developed hybrid slot antenna structures for microbial sensing in the THz frequency range, where silver nanowires (AgNWs) were employed to increase the sensitivity. In order to fabricate the hybrid devices, we partially etched the AgNW in the slot antenna region, where we can expect the field enhancement effect at ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33617-2
更新日期：2018-10-19 00:00:00
abstract：:Diagnostic criteria for pathological gambling and alcohol dependence (AD) include repeated addictive behavior despite severe negative consequences. However, the concept of loss aversion (LA) as a facet of value-based decision making has not yet been used to directly compare these disorders. We hypothesized reduced LA ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16433-y
更新日期：2017-11-24 00:00:00
abstract：:Biological armors such as mollusk shells have long been recognized and studied for their values in inspiring novel designs of engineering materials with higher toughness and strength. However, no material is invincible and biological armors also have their rivals. In this paper, our attention is focused on the teeth o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23509
更新日期：2016-03-22 00:00:00
abstract：:Exposure to stress during early life affects subsequent behaviors and increases the vulnerability to adult pathologies, a phenomenon that has been well documented in humans and rodents. In this study, we introduce a chronic unpredictable stress protocol adapted to young zebrafish, which is an increasingly popular vert...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67182-4
更新日期：2020-06-25 00:00:00
abstract：:Being one of the most common trees in forests, Pinus sylvestris L. is a frequently used raw material for wood products. Its specific odour is, however, mostly unresolved to date. Accordingly, we investigated Scots pine wood samples grown in Germany for their main odorant composition. We employed dedicated odorant anal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26626-8
更新日期：2018-05-29 00:00:00
abstract：:In the European Union, the use of recombinant bovine somatotropin (rbST) in dairy cattle is forbidden. Monitoring rbST (ab)use by its direct detection in animal matrices still remains a challenging task. New monitoring methods based on indirect detection of the substance are necessary. A new transcriptomic system base...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41343-6
更新日期：2019-03-18 00:00:00
abstract：:X-ray free-electron lasers (XFELs) provide extremely bright and highly spatially coherent x-ray radiation with femtosecond pulse duration. Currently, they are widely used in biology and material science. Knowledge of the XFEL statistical properties during an experiment may be vitally important for the accurate interpr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19793-1
更新日期：2018-02-02 00:00:00
abstract：:Experimental evidence supports that signaling pathways can induce different dynamics of transcription factor (TF) activation, but how an input signal is encoded by such a dynamic, noisy TF and further decoded by downstream genes remains largely unclear. Here, using a system of stochastic transcription with signal regu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42128
更新日期：2017-02-08 00:00:00
abstract：:Using dedicated contrast agents high-quality X-ray imaging of soft tissue structures with isotropic micrometre resolution has become feasible. This technique is frequently titled as virtual histology as it allows production of slices of tissue without destroying the sample. The use of contrast agents is, however, ofte...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30385
更新日期：2016-07-27 00:00:00
abstract：:The effects of surface treatment combining corona discharge and hydrogen peroxide (H2O2) on the electrochemical performance of carbon felt electrodes for vanadium redox flow batteries (VRFBs) have been thoroughly investigated. A high concentration of oxygen functional groups has been successfully introduced onto the s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06906
更新日期：2014-11-04 00:00:00
abstract：:A novel flexible electret membrane, exhibiting persistent electrostatic effect, distinctive temperature stability and outstanding capability of resistance to harsh environment and fatigue, is demonstrated by experiment. Its excellent electret performance is correlated to the synergy of three factors, which are space c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07747-y
更新日期：2017-08-16 00:00:00
abstract：:The aims of this study were, firstly, to assess the effect of concurrent peripheral artery disease (PAD) on the health-related quality of life (QOL) of people diagnosed with a small abdominal aortic aneurysm (AAA); and secondly, to test whether the peroxisome proliferator-activated receptor α agonist fenofibrate impro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71454-4
更新日期：2020-09-03 00:00:00
abstract：:Tau is a Microtubule-associated protein that induces and stabilizes the formation of the Microtubule cytoskeleton and plays an important role in neurodegenerative diseases. The Microtubules binding region of Tau has been determined for a long time but where and how Tau binds to its partner still remain a topic of deba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-32096-9
更新日期：2018-09-14 00:00:00
abstract：:Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02863
更新日期：2013-10-04 00:00:00
abstract：:The generation of novelty is central to any creative endeavor. Novelty generation and the relationship between novelty and individual hedonic value have long been subjects of study in social psychology. However, few studies have utilized large-scale datasets to quantitatively investigate these issues. Here we consider...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02758
更新日期：2013-09-26 00:00:00
abstract：:The mechanistic target of rapamycin (mTOR) is a central mediator of protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation in human skeletal muscle in the basal state as well as immediately, 1 and 3 h after an acute bout o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05483-x
更新日期：2017-07-10 00:00:00
