Abstract：
:The interaction between the T cell antigen receptor (TCR) and antigenic peptide in complex with major histocompatibility complex (MHC) molecules is a crucial step in T cell activation. The relative contributions of TCR:peptide and TCR:MHC contacts to the overall binding energy remain unclear. This has important implications for our understanding of T cell development and function. In this study we used site directed mutagenesis to estimate the contribution of HLA-A2 side-chains to the binding of four TCRs. Our results show that these TCRs have very different energetic 'footprints' on HLA-A2, with no residues contributing to all TCR interactions. The estimated overall contribution of MHC side-chains to the total interaction energy was variable, with lower limits ranging from 11% to 50%. Kinetic analysis suggested a minor and variable contribution of MHC side-chains to the transition state complex, arguing against a two-step mechanism for TCR binding.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhang H,Lim HS,Knapp B,Deane CM,Aleksic M,Dushek O,van der Merwe PAdoi
10.1038/srep35326subject
Has Abstractpub_date
2016-10-13 00:00:00pages
35326issn
2045-2322pii
srep35326journal_volume
6pub_type
杂志文章abstract：:Patients with Idiopathic Parkinson's Disease (PD) have an increased risk for fractures. Currently, many studies have reported inferior outcomes in PD patients after orthopedic procedures. However, there are very few studies assessing the outcome of upper extremity fractures (UEF) in PD patients. In this study, we revi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78168-7
更新日期：2020-12-03 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-59025-z
更新日期：2020-02-07 00:00:00
abstract：:Because of inadequate nutrient and water supply, soils are often unproductive in Northwest China. We studied the effects of manure application at low (LM 7.5 t ha(-1)), medium (MM 15 t ha(-1)), and high (HM 22.5 t ha(-1)) rates combined with fixed levels of chemical fertilizers on maize growth and rainfall use effici...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20994
更新日期：2016-02-12 00:00:00
abstract：:Worldwide variation in human stature and limb proportions is widely accepted to reflect thermal adaptation, but the contribution of population history to this variation is unknown. Furthermore, stature and relative lower limb length (LLL) show substantial plastic responses to environmental stressors, e.g., nutrition, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79501-w
更新日期：2021-01-11 00:00:00
abstract：:HIV-1 Tat protein is released from HIV-1-infected cells and can enter non-permissive cells including neurons. Tat disrupts neuronal homeostasis and may contribute to the neuropathogenesis in people living with HIV (PLWH). The use of cocaine by PLWH exacerbates neuronal dysfunction. Here, we examined the mechanisms by ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34539-9
更新日期：2018-11-02 00:00:00
abstract：:Chronic hepatitis viral infection, alcoholic intoxication, and obesity cause liver fibrosis, which progresses to decompensated liver cirrhosis, a disease for which medical demands cannot be met. Since there are currently no approved anti-fibrotic therapies for established liver fibrosis, the development of novel modal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43298-0
更新日期：2019-05-02 00:00:00
abstract：:Although the glucose lowering effect of dipeptidyl peptidase-4 (DPP4) inhibitors is well established, several potential serious acute safety concerns have been raised including acute kidney injury, respiratory tract infections, and acute pancreatitis. Using the UK-based Clinical Practice Research Datalink (CPRD), we i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33483-y
更新日期：2018-10-11 00:00:00
abstract：:DNA-based data storage has emerged as a promising method to satisfy the exponentially increasing demand for information storage. However, practical implementation of DNA-based data storage remains a challenge because of the high cost of data writing through DNA synthesis. Here, we propose the use of degenerate bases a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43105-w
更新日期：2019-04-29 00:00:00
abstract：:The SW Japan arc built by subduction of the Philippine Sea (PHS) plate exhibits uneven distribution of volcanoes: thirteen Quaternary composite volcanoes form in the western half of this arc, Kyushu Island, while only two in the eastern half, Chugoku district. Reconstruction of the PHS plate back to 14 Ma, together wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72173-6
更新日期：2020-09-14 00:00:00
abstract：:This study presents analysis of four chimney samples in terms of glycerol dialkyl glycerol tetraether lipids (GDGTs), representing different growing stages of sulfide chimneys at the Deyin hydrothermal field, the southern mid-Atlantic ridge. The modified Bligh-Dyer method was used for lipid extraction and purification...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26166-1
更新日期：2018-05-23 00:00:00
abstract：:This study aimed to design and functionally characterize peptide mimetics of the nanobody (Nb) related to the β2-adrenergic receptor (β2-AR) (nanobody-derived peptide, NDP). We postulated that the computationally derived and optimized complementarity-determining region 3 (CDR3) of Nb is sufficient for its interaction ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52934-8
更新日期：2019-11-12 00:00:00
abstract：:Network robustness represents the ability of networks to withstand failures and perturbations. In social networks, maintenance of individual activities, also called persistence, is significant towards understanding robustness. Previous works usually consider persistence on pre-generated network structures; while in so...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42956
更新日期：2017-02-21 00:00:00
abstract：:Ovarian cancer is one of the leading causes of death in gynecological malignancies, and the resistance to chemotherapeutic agents remains a major challenge to successful ovarian cancer chemotherapy. Dihydromyricetin (DHM), a natural flavonoid derived from Ampeopsis Grossdentata, has been widely applied in food industr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46060
更新日期：2017-04-24 00:00:00
abstract：:We estimated the prevalence of anemia among school children and investigated factors associated with this problem in Kuwait. A cross-sectional study was conducted on 1415 adolescents randomly selected from middle schools in Kuwait. Hemoglobin, iron, ferritin, folate and vitamin B12, in addition to many other laborator...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60816-7
更新日期：2020-04-03 00:00:00
abstract：:Bone fracture repair is a multifaceted, coordinated physiological process that requires new bone formation and resorption, eventually returning the fractured bone to its original state. Currently, a variety of different approaches are pursued to accelerate the repair of defective bones, which include the use of 'gold ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67886-7
更新日期：2020-07-14 00:00:00
abstract：:This study aimed to develop and validate a model for the preoperative prediction of the effectiveness of hysteroscopic resection of a uterine cesarean niche in patients with postmenstrual spotting. The predictive model was developed in a primary prospective cohort consisting of 208 patients with niche treated by hyste...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74622-8
更新日期：2020-10-15 00:00:00
abstract：:Post-operative pancreatic fistula (POPF) following pancreatic resection is a life-threatening surgical complication. Cell sheets were prepared and harvested using temperature-responsive culture dishes and transplanted as patches to seal POPF. Two different mesenchymal stem cell (MSC) sheets were compared in terms of t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18490-9
更新日期：2018-01-10 00:00:00
abstract：:Misuse of antibiotics in the clinical and agricultural sectors has caused the emergence of multidrug-resistant (MDR) Klebsiella pneumoniae which contributes a threat to human health. In this study, we assessed the feasibility of lavender essential oil (LVO) as an antimicrobial agent in combinatory therapy with meropen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55601-0
更新日期：2020-01-21 00:00:00
abstract：:Objectum-sexuality (OS) is a sexual orientation which has received little attention in the academic literature. Individuals who identify as OS experience emotional, romantic and/or sexual feelings towards inanimate objects (e.g. a bridge, a statue). We tested 34 OS individuals and 88 controls, and provide the first em...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56449-0
更新日期：2019-12-27 00:00:00
abstract：:Carotenoids are anti-oxidative agents. Human skin and eyes contain specific carotenoid species known to prevent various pathologies caused by oxidative stress. We quantified skin and eye carotenoid levels and investigated their potential correlation in a population including 985 Japanese patients and staff members of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45751-6
更新日期：2019-06-27 00:00:00
abstract：:LED-based photoacoustic imaging has practical value in that it is affordable and rugged; however, this technology has largely been confined to anatomic imaging with limited applications into functional or molecular imaging. Here, we report molecular imaging reactive oxygen and nitrogen species (RONS) with a near-infra...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47599-2
更新日期：2019-08-06 00:00:00
abstract：:The challenge in the treatment of glioblastoma is the failure to identify the cancer invasive area outside the contrast-enhancing tumour which leads to the high local progression rate. Our study aims to identify its progression from the preoperative MR radiomics. 57 newly diagnosed cerebral glioblastoma patients were ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66691-6
更新日期：2020-06-16 00:00:00
abstract：:Density functional theory predictions have been combined with the microwave-plasma chemical vapor deposition technique to explore metastable synthesis of boron-rich boron-carbide materials. A thin film synthesis of high-hardness (up to 37 GPa) B50C2 via chemical vapor deposition was achieved. Characterization of the e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61462-9
更新日期：2020-03-10 00:00:00
abstract：:Soil microbial responses to fire are likely to change over the course of forest recovery. Investigations on long-term changes in bacterial dynamics following fire are rare. We characterized the soil bacterial communities across three different times post fire in a 2 to 152-year fire chronosequence by Illumina MiSeq se...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32411
更新日期：2016-08-30 00:00:00
abstract：:Neonatal mice exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). Cardiac biomechanics intricately affect long-term heart function, but whether regenerated cardiac muscle is biomechanically similar to native myocardium remains u...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63324-w
更新日期：2020-04-30 00:00:00
abstract：:Panax ginseng (GS) and Veratrum nigrum (VN) are representative of incompatible pairs in "eighteen antagonistic medicaments" that have been recorded in the Chinese medicinal literature for over 2,000 years. However, evidence linking interference effects with combination use is scare. Based on the estrogen-like effect o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26924
更新日期：2016-05-27 00:00:00
abstract：:To realize power efficient solution-processed phosphorescent organic light-emitting diodes (s-PhOLEDs), the corresponding high driving voltage issue should be well solved. To solve it, efforts have been devoted to the exploitation of novel host or interfacial materials. However, the issues of charge trapping of phosph...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12487
更新日期：2015-07-24 00:00:00
abstract：:Marine pollution is a significant issue in recent decades, with the increase in industries and their waste harming the environment and ecosystems. Notably, the rise in shellfish industries contributes to tons of shellfish waste composed of up to 58% chitin. Chitin, the second most ample polymer next to cellulose, is i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-68772-y
更新日期：2020-07-17 00:00:00
abstract：:Monitoring ecological changes in marine ecosystems is expensive and time-consuming. Passive acoustic methods provide continuous monitoring of soniferous species, are relatively inexpensive, and can be integrated into a larger network to provide enhanced spatial and temporal coverage of ecological events. We demonstrat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74647-z
更新日期：2020-10-20 00:00:00
abstract：:In recent pioneer experiment, a strong spin-orbit coupling, with equal Rashba and Dresselhaus strengths, has been created in a trapped Bose-Einstein condensate. Moreover, many exotic superfluid phenomena induced by this strong spin-orbit coupling have been predicted. In this report, we show that this novel spin-orbit ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03166
更新日期：2013-11-07 00:00:00
