Abstract:
:Diabetes is caused by the loss or dysfunction of insulin-secreting β-cells in the pancreas. β-cells reduce their mass and lose insulin-producing ability in vitro, likely due to insufficient cell-cell and cell-extracellular matrix (ECM) interactions as β-cells lose their native microenvironment. Herein, we built an ex-vivo cell microenvironment by culturing primary β-cells in direct contact with 'synthetic neighbors', cell-sized soft polymer microbeads that were modified with cell-cell signaling factors as well as components from pancreatic-tissue-specific ECMs. This biomimetic 3D microenvironment was able to promote native cell-cell and cell-ECM interactions. We obtained sustained maintenance of β-cell function in vitro enhanced cell viability from the few days usually observed in 2D culture to periods exceeding three weeks, with enhanced β-cell stability and insulin production. Our approach can be extended to create a general 3D culture platform for other cell types.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Li W,Lee S,Ma M,Kim SM,Guye P,Pancoast JR,Anderson DG,Weiss R,Lee RT,Hammond PTdoi
10.1038/srep02863subject
Has Abstractpub_date
2013-10-04 00:00:00pages
2863issn
2045-2322pii
srep02863journal_volume
3pub_type
杂志文章abstract::Ferritin has gained significant attention as a potential reporter gene for in vivo imaging by magnetic resonance imaging (MRI). However, due to the ferritin ferrihydrite core, the relaxivity and sensitivity for detection of native ferritin is relatively low. We report here on a novel chimeric magneto-ferritin reporter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26550
更新日期:2016-05-23 00:00:00
abstract::Chronic inflammation-associated bone destruction, which is observed in rheumatoid arthritis (RA) and periodontitis, is mediated by excessive osteoclastogenesis. We showed previously that γ-glutamyltranspeptidase (GGT), an enzyme involved in glutathione metabolism, acts as an endogenous activator of such pathological o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35930
更新日期:2016-10-24 00:00:00
abstract::As an important method for building blocks synthesis, whole cell biocatalysis is hindered by some shortcomings such as unpredictability of reactions, utilization of opportunistic pathogen, and side reactions. Due to its biological and extensively studied genetic background, Pseudomonas putida KT2440 is viewed as a pro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06939
更新日期:2014-11-06 00:00:00
abstract::Thermal rectifiers whose forward heat fluxes are greater than reverse counterparts have been extensively studied. Here we have discovered, idealized, and derived the ultimate limit of such rectification ratios, which are partially validated by numerical simulations, experiments, and micro-scale Hamiltonian-oscillator ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12677
更新日期:2015-08-04 00:00:00
abstract::Intravitreal anti-vascular endothelial growth factor (VEGF) agents have revolutionized the treatment of retinopathy of prematurity (ROP); however, there are concerns regarding the potential systemic complications caused by those treatments. This study aimed to determine the serum concentrations of cytokines in infants...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69684-7
更新日期:2020-07-29 00:00:00
abstract::Both CC and CXC-class chemokines drive inflammatory disease. Tick salivary chemokine-binding proteins (CKBPs), or evasins, specifically bind subsets of CC- or CXC-chemokines, and could precisely target disease-relevant chemokines. Here we have used yeast surface display to identify two tick evasins: a CC-CKBP, P1243 f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24568-9
更新日期:2018-04-20 00:00:00
abstract::Over-exploitation of groundwater and marked changes in climate over the years have imposed immense pressure on the global groundwater resources. As demand of potable water increases across the globe for human consumption, agriculture and industrial uses, the need to evaluate the groundwater potential and productivity ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38567-x
更新日期:2019-02-14 00:00:00
abstract::Structural materials used for safety critical applications require high strength and simultaneously high resistance against crack growth, referred to as damage tolerance. However, the two properties typically exclude each other and research efforts towards ever stronger materials are hampered by drastic loss of fractu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33228
更新日期:2016-09-14 00:00:00
abstract::Angiogenesis is one of the hallmarks of cancer and, as such, one of the alternative general targets for anticancer therapy. Since angiogenesis is a complex process involving a high number of interconnected components, a network approach would be a convenient systemic way to analyse responses to directed drug attacks. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00061
更新日期:2011-01-01 00:00:00
abstract::One of the main azole-resistance mechanisms in Candida pathogens is the upregulation of drug efflux pumps, which compromises the efficacy of azoles and results in treatment failure. The combination of azole-antifungal agents with efflux pump inhibitors represents a promising strategy to combat fungal infection. High-t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33687
更新日期:2016-09-21 00:00:00
abstract::Environmental stimuli have the ability to generate specific representations of the rewards they predict and in so doing alter the selection and performance of reward-seeking actions. The basolateral amygdala participates in this process, but precisely how is unknown. To rectify this, we monitored, in near-real time, b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12511
更新日期:2015-07-27 00:00:00
abstract::Natural biochemical systems are ubiquitously organized both in space and time. Engineering the spatial organization of biochemistry has emerged as a key theme of synthetic biology, with numerous technologies promising improved biosynthetic pathway performance. One strategy, however, may produce disparate results for d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26399-0
更新日期:2018-05-29 00:00:00
abstract::This cross-sectional study aimed to investigate the prevalence and risk factors of chronic kidney disease (CKD) among 1,096 primary care type 2 diabetes (T2DM) patients in northern Thailand between October 2016 and September 2017. CKD was defined as estimated glomerular rate filtration values of <60 mL/min/1.73 m2. Pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63443-4
更新日期:2020-04-10 00:00:00
abstract::A new and facile approach to selectively functionalize the internal and external surfaces of porous silicon (pSi) for drug delivery applications is reported. To provide a surface that is suitable for sustained drug release of the hydrophobic cancer chemotherapy drug camptothecin (CPT), the internal surfaces of pSi fil...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37750-w
更新日期:2019-02-04 00:00:00
abstract::Identification of hot spots, a small portion of protein-protein interface residues that contribute the majority of the binding free energy, can provide crucial information for understanding the function of proteins and studying their interactions. Based on our previous method (PredHS), we propose a new computational a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32511-1
更新日期:2018-09-24 00:00:00
abstract::The Fenton-like activity of nanoceria has attracted intensive attention for wastewater treatment in recent years. During the Fenton-like reaction, the adsorption of organic pollutants on catalyst surface plays a key role in their degradation. In this work, the adsorption-degradation of methylene blue (MB) and Congo re...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36794-2
更新日期:2019-03-21 00:00:00
abstract::Friction is a major inhibitor in almost every mechanical system. Enlightened by the Leidenfrost effect - a droplet can be levitated by its own vapor layer on a sufficiently hot surface - we demonstrate for the first time that a small cart can also be levitated by Leidenfrost vapor. The levitated cart can carry certain...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00797
更新日期:2012-01-01 00:00:00
abstract::Monitoring one's actions is essential for goal-directed performance. In the event-related potential (ERP), errors are followed by fronto-centrally distributed negativities. These error(-related) negativity (Ne/ERN) amplitudes are often found to be attenuated in patients with Parkinson's disease (PD) compared to health...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41222
更新日期:2017-01-24 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-24098-4
更新日期:2018-04-12 00:00:00
abstract::Voice disorder such as vocal fatigue is a common and complex multifaceted clinical problem that presents a significant impact on quality of life. In this study, the functional near-infrared diffuse optical technique (fNIRS-DOT) was proposed as a novel approach for human vocal cords oxidative metabolism detection and a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16807-2
更新日期:2017-12-05 00:00:00
abstract::Secondary thrombocytosis (ST) is frequently observed in children with a variety of clinical conditions. The leading cause of ST is respiratory tract infection (RTI) in children. Nasopharyngeal aspirate samples were collected and assessed for common respiratory viruses. The relationships between virus infections and se...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22964
更新日期:2016-03-11 00:00:00
abstract::Glucagon-like peptide-1 (GLP-1) promotes insulin secretion from pancreatic β-cells in a glucose dependent manner. Several pathways mediate this action by rapid, kinase phosphorylation-dependent, but gene expression-independent mechanisms. Since GLP-1-induced insulin secretion requires glucose metabolism, we aimed to a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02838-2
更新日期:2017-06-01 00:00:00
abstract::Pre-operative discrimination of malignant masses is crucial for accurate diagnosis and prompt referral to a gynae oncology centre for optimal surgical intervention. HGSOC progression is correlated with local and systemic inflammation. We hypothesised that inclusion of inflammatory biomarkers in sera may improve diagno...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59009-z
更新日期:2020-02-10 00:00:00
abstract::Despite the frequent use of ionising radiation (IR) in therapy and diagnostics and the unavoidable exposure to external radiation sources, our knowledge regarding the radiosensitivity of human blood cell populations is limited and published data, obtained under different experimental conditions, are heterogeneous. To ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81058-1
更新日期:2021-01-28 00:00:00
abstract::The hitherto difficult task of reliably estimating populations of wide-ranging megafauna has been enabled by advances in capture-recapture methodology. Here we combine photographic sampling with a Bayesian spatially-explicit capture-recapture (SCR) model to estimate population parameters for the endangered Asian eleph...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44795-y
更新日期:2019-06-12 00:00:00
abstract::Gliomas are highly invasive brain tumours characterised by poor prognosis and limited response to therapy. There is an ongoing debate on the therapeutic potential of vaso-modulatory interventions against glioma invasion. Prominent vasculature-targeting therapies involve tumour blood vessel deterioration and normalisat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37283
更新日期:2016-11-23 00:00:00
abstract::Brain hypometabolism is a common epilepsy-related finding in both patients and animal models. Fluorodeoxyglucose positron emission tomography studies have shown that recurrent seizures lead to reduced glucose metabolism in certain brain regions, but no studies have definitively determined whether this induces epilepto...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05038-0
更新日期:2017-07-19 00:00:00
abstract::Traumatic brain injury (TBI) is a risk factor for Alzheimer's disease (AD), although the mechanisms contributing to this increased risk are unknown. Insulin resistance is an additional risk factor for AD whereby decreased insulin signaling increases synaptic sensitivity to amyloid beta (Aβ) and tau. Considering this, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44635-z
更新日期:2019-06-03 00:00:00
abstract::Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows simultaneous imaging of numerous RNA species in their native cellular environment and hence spatially resolved single-cell transcriptomic measurements. However, the relatively modest brightness of signals from single RNA molecules can become ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43943-8
更新日期:2019-05-22 00:00:00
abstract::The success of filamentous fungi in colonizing most natural environments can be largely attributed to their ability to form an expanding interconnected network, the mycelium, or thallus, constituted by a collection of hyphal apexes in motion producing hyphae and subject to branching and fusion. In this work, we charac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57808-y
更新日期:2020-02-21 00:00:00