Abstract:
:As an important method for building blocks synthesis, whole cell biocatalysis is hindered by some shortcomings such as unpredictability of reactions, utilization of opportunistic pathogen, and side reactions. Due to its biological and extensively studied genetic background, Pseudomonas putida KT2440 is viewed as a promising host for construction of efficient biocatalysts. After analysis and reconstruction of the lactate utilization system in the P. putida strain, a novel biocatalyst that only exhibited NAD-independent D-lactate dehydrogenase activity was prepared and used in L-2-hydroxy-carboxylates production. Since the side reaction catalyzed by the NAD-independent L-lactate dehydrogenase was eliminated in whole cells of recombinant P. putida KT2440, two important L-2-hydroxy-carboxylates (L-lactate and L-2-hydroxybutyrate) were produced in high yield and high optical purity by kinetic resolution of racemic 2-hydroxy carboxylic acids. The results highlight the promise in biocatalysis by the biotechnologically important organism P. putida KT2440 through genomic analysis and recombination.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wang Y,Lv M,Zhang Y,Xiao X,Jiang T,Zhang W,Hu C,Gao C,Ma C,Xu Pdoi
10.1038/srep06939subject
Has Abstractpub_date
2014-11-06 00:00:00pages
6939issn
2045-2322pii
srep06939journal_volume
4pub_type
杂志文章abstract::The temperate bamboos (tribe Arundinarieae, Poaceae) are strongly supported as monophyly in recent molecular studies, but taxonomic delineation and phylogenetic relationships within the tribe lack resolution. Here, we sampled 39 species (36 temperate bamboos and 3 outgroups) for restriction-site associated DNA sequenc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11367-x
更新日期:2017-09-14 00:00:00
abstract::Our study focused on quantifying the alterations of streamflow at a weir site due to the construction of a mini-hydropower plant in the Gurugoda Oya (Sri Lanka), and evaluating the spatial responses of benthic macroinvertebrates to altered flow regime. The HEC-HMS 3.5 model was applied to the Gurugoda Oya sub-catchmen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79576-5
更新日期:2021-01-12 00:00:00
abstract::Dehydration (water loss >2.0% of body weight) has significant negative effects on physical and mental performance. In two studies the effects of minor hypo-hydration (water loss <1.0% of body weight) on CNS function, mood and cardiovascular functioning were measured. Study 1: On two mornings twelve male participants w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52775-5
更新日期:2019-11-11 00:00:00
abstract::In recent years it has been shown that the therapeutic benefits of human mesenchymal stem/stromal cells (hMSCs) in the Central Nervous System (CNS) are mainly attributed to their secretome. The implementation of computer-controlled suspension bioreactors has shown to be a viable route for the expansion of these cells ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27791
更新日期:2016-06-15 00:00:00
abstract::The prominent comprehensive properties of solid-solution- and intermetallic-based Ti alloys are derived from their diverse microstructures induced by multi-component alloying, which results in a chemical composition complexity. A cluster-plus-glue-atom model, characterizing the chemical short-range orders, was introdu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40302-5
更新日期:2019-03-04 00:00:00
abstract::The histological spectrum of nonalcoholic fatty liver diseases (NAFLD) ranges from hepatic steatosis to steatohepatitis and fibrosis. Berberine (BBR) is known for its therapeutic effect on obesity, hyperglycaemia and dyslipidaemia; however, its effect on NAFLD has yet to be thoroughly explored. Db/db mice and methioni...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20848
更新日期:2016-02-09 00:00:00
abstract::Plants are key determinants of soil microbial community (SMC). Legumes and grasses are distinct groups in various ecosystems; however, how they differentially shape SMC structure and functioning has yet to be explored. Here, we investigate SMC in soils grown with stylo (legume) or bahiagrass (grass). Soil metagenomic ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10613-6
更新日期:2017-08-31 00:00:00
abstract::Adenocarcinoma, large cell carcinoma and squamous cell carcinoma are the most commonly diagnosed subtypes of non-small cell lung cancers (NSCLC). Numerous lung cancer cell types have exhibited electrotaxis under direct current electric fields (dcEF). Physiological electric fields (EF) play key roles in cancer cell mig...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44505-8
更新日期:2019-05-30 00:00:00
abstract::Decreasing coral cover on the Great Barrier Reef (GBR) may provide opportunities for rapid growth and expansion of other taxa. The bioeroding sponges Cliona spp. are strong competitors for space and may take advantage of coral bleaching, damage, and mortality. Benthic surveys of the inshore GBR (2005-2014) revealed th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02196-z
更新日期:2017-06-02 00:00:00
abstract::The association between cancer and volatile organic metabolites in exhaled breaths has attracted increasing attention from researchers. The present study reports on a systematic study of gas profiles of metabolites in human exhaled breath by pattern recognition methods. Exhaled breath was collected from 85 patients wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05383
更新日期:2014-06-20 00:00:00
abstract::Haber-Bosch nitrogen (N) has been increasingly used in industrial products, e.g., nylon, besides fertilizer. Massive numbers of species of industrial reactive N (Nr) have emerged and produced definite consequences but receive little notice. Based on a comprehensive inventory, we show that (1) the industrial N flux has...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02579
更新日期:2013-01-01 00:00:00
abstract::Nanometric biocompatible Metal-Organic Frameworks (nanoMOFs) are promising candidates for drug delivery. Up to now, most studies have targeted the intravenous route, related to pain and severe complications; whereas nanoMOFs for oral administration, a commonly used non-invasive and simpler route, remains however unexp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43099
更新日期:2017-03-03 00:00:00
abstract::Rassf1A/5 tumor suppressors serve as adaptor proteins possessing a modular architecture with the C-terminal consisting of a coiled-coil SARAH (Salvador-Rassf-Hippo) domain and the central portion being composed of Ras associated (RA) domain. Here, we investigate the effect of Rassf effectors on Mst1 function by mappin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45020
更新日期:2017-03-22 00:00:00
abstract::Understanding the chemical and physical properties of metal/graphene oxide (M/GO) interfaces is important when GO is used in electronic and electrochemical devices because the metal layer must be firmly attached to GO. Here, permeation of metal from the surface into GO paper bulk at the M/GO interface was observed at ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03647
更新日期:2014-01-13 00:00:00
abstract::Patients with Type-2 Diabetes Mellitus (T2DM) have a considerably higher risk of developing mild cognitive impairment (MCI) and dementia. The initial symptoms are very insidious at onset. We investigated the alterations in spontaneous brain activity and network connectivity through regional homogeneity (ReHo) and grap...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76495-3
更新日期:2020-12-04 00:00:00
abstract::The future Internet is very likely the mixture of all-optical Internet with low power consumption and quantum Internet with absolute security guaranteed by the laws of quantum mechanics. Photons would be used for processing, routing and com-munication of data, and photonic transistor using a weak light to control a st...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45582
更新日期:2017-03-28 00:00:00
abstract::Sacha inchi (Plukenetia volubilis L.) is a shrub native to Amazon rainforests that's of commercial interest as its seeds contain 35-60% edible oil (dry weight). This oil is one of the healthiest vegetable oils due to its high polyunsaturated fatty acid content and favourable ratio of omega-6 to omega-3 fatty acids. De...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77177-w
更新日期:2020-11-24 00:00:00
abstract::Pooled data analysis in the field of maternal and child nutrition rarely incorporates data from low- and middle-income countries and existing studies lack a description of the methods used to harmonize the data and to assess heterogeneity. We describe the creation of the Brazilian Maternal and Child Nutrition Consorti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71612-8
更新日期:2020-09-10 00:00:00
abstract::One procedure widely used to detect the velocity of a moving object is by using the Doppler effect. This is the perceived change in frequency of a wave caused by the relative motion between the emitter and the detector, or between the detector and a reflecting target. The relative movement, in turn, generates a time-v...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep02815
更新日期:2013-10-02 00:00:00
abstract::This study aims to understand through statistical learning the basic biophysical mechanisms behind three-dimensional folding of epigenomes. The 3DEpiLoop algorithm predicts three-dimensional chromatin looping interactions within topologically associating domains (TADs) from one-dimensional epigenomics and transcriptio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23276-8
更新日期:2018-03-26 00:00:00
abstract::Shigella spp., emerging from multiple origins of Escherichia coli, poses a significant health threat as a causative agent of bacillary dysentery. While multiple serotypes of four different species have evolved via independent lineages, Shigella spp. are designated as a single pathotype, primarily because of their comm...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39810-1
更新日期:2019-03-01 00:00:00
abstract::The formation of proper sensory afferent connections during development is essential for brain function. Activity-based competition is believed to drive ocular dominance columns (ODC) in mammals and in experimentally-generated three-eyed frogs. ODC formation is thus a compromise of activity differences between two eye...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08338
更新日期:2015-02-09 00:00:00
abstract::Neisseria meningitidis is a human-specific bacterium that varies in invasive potential. All meningococci are carried in the nasopharynx, and most genotypes are very infrequently associated with invasive meningococcal disease; however, those belonging to the 'hyperinvasive lineages' are more frequently associated with ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21015
更新日期:2016-02-12 00:00:00
abstract::Manual count of mitotic figures, which is determined in the tumor region with the highest mitotic activity, is a key parameter of most tumor grading schemes. It can be, however, strongly dependent on the area selection due to uneven mitotic figure distribution in the tumor section. We aimed to assess the question, how...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73246-2
更新日期:2020-10-05 00:00:00
abstract::Male Wistar rats were exposed to herbicide, pendimethalin (PND) at varying oral doses of 62.5, 125 and 250 mg/kg b.w. for 14 days. Toxiological effects were assessed in terms of oxidative stress, DNA damage, histopathological alterations and induction of anti-inflammatory and apoptotic responses linked Bax, Bcl-2, IFN...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35484-3
更新日期:2018-11-20 00:00:00
abstract::The aims of this study were as follows: to estimate the mortality and years of life lost, assessed by disability-adjusted life years (DALYs), due to breast cancer attributable to physical inactivity in Brazilian women; to compare the estimates attributable to physical inactivity and to other modifiable risk factors; a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29467-7
更新日期:2018-07-24 00:00:00
abstract::The Monte Carlo (MC) method is widely used to solve various problems in radiotherapy. There has been an impetus to accelerate MC simulation on GPUs whereas thread divergence remains a major issue for MC codes based on acceptance-rejection sampling. Inverse transform sampling has the potential to eliminate thread diver...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66844-7
更新日期:2020-06-17 00:00:00
abstract::Vocal expression of emotions has been suggested to be conserved throughout evolution. However, since vocal indicators of emotions have never been compared between closely related species using similar methods, it remains unclear whether this is the case. Here, we investigated vocal indicators of emotional valence (neg...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09437-1
更新日期:2017-08-18 00:00:00
abstract::Marine pollution is a significant issue in recent decades, with the increase in industries and their waste harming the environment and ecosystems. Notably, the rise in shellfish industries contributes to tons of shellfish waste composed of up to 58% chitin. Chitin, the second most ample polymer next to cellulose, is i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68772-y
更新日期:2020-07-17 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-63873-0
更新日期:2020-04-17 00:00:00