Abstract:
OBJECTIVES:Transglutaminase-2 (TGM-2) protein is involved in the cross-linking of matrix proteins resulting in several fibrotic disorders and can be induced by reactive oxygen species (ROS). Little is known about its role in the development of oral submucocal fibrosis (OSF). Hence, we hypothesize that TGM-2 may have a role in the pathogenesis of areca quid chewing-associated OSF and arecoline, a major areca nut alkaloid, could regulate TGM-2 via ROS generation. MATERIALS:Forty OSF specimens from areca quid chewing-associated OSF and ten normal buccal mucosa biopsy samples without areca quid chewing were analyzed by immunohistochemistry. The expression of TGM-2 from fibroblasts cultured from OSF and normal buccal mucosa was evaluated by Western blot. The effect of arecoline on normal buccal mucosa fibroblasts (BMFs) was used to elucidate whether TGM-2 expression could be affected by arecoline by using 2', 7'-dichlorofluorescein diacetate assay and Western blot. In addition, glutathione precursor N-acetyl-L-cysteine (NAC) and epigallocatechin-3 gallate (EGCG) were added to find the possible regulatory mechanisms. RESULTS:TGM-2 expression was significantly higher in OSF specimens than normal specimens (p < 0.05). Fibroblasts derived from OSF were found to exhibit higher TGM-2 expression than BMFs in protein levels (p < 0.05). Arecoline significantly upregulated the intracellular ROS generation in a dose-dependent manner (p < 0.05). TGM-2 protein induced by arecoline was found in BMFs in a dose-dependent manner (p < 0.05). Treatment with NAC and EGCG markedly inhibited TGM-2 expression induced by arecoline (p < 0.05). CONCLUSIONS:Our results suggest that TGM-2 expression is significantly upregulated in OSF tissues from areca quid chewers. Arecoline-upregulated TGM-2 expression may be mediated by ROS generation. CLINICAL RELEVANCE:TGM-2 protein is upregulated in areca quid chewing-associated OSF. Using this in vitro model, antioxidants could inhibit arecoline-upregulated TGM-2 expression. NAC and EGCG may serve as a useful agent in controlling OSF.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
Lee SS,Chen YJ,Tsai CH,Huang FM,Chang YCdoi
10.1007/s00784-015-1579-0subject
Has Abstractpub_date
2016-06-01 00:00:00pages
1029-34issue
5eissn
1432-6981issn
1436-3771pii
10.1007/s00784-015-1579-0journal_volume
20pub_type
杂志文章abstract::From a preventive point of view collection of data concerning carious and non-carious cervical tooth defects is definitely important. Consequently, the prevalence and distribution of different root lesions were studied and correlated with behavioral and biological factors in 50- to 60-year-old German individuals (n = ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050097
更新日期:1999-12-01 00:00:00
abstract:OBJECTIVES:To assess the quality of clinical practice guidelines (CPGs) on screening and diagnosis of oral cancer and to describe the characteristics of their recommendations. MATERIALS AND METHODS:We systematically searched EMBASE, MEDLINE, CPG' websites, and dentistry and oncology scientific societies to identify CP...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2668-7
更新日期:2019-05-01 00:00:00
abstract::Aims of the study were (1) to establish a method for quantification of chlorhexidine (CHX) in small volumes and (2) to determine CHX release from differently concentrated CHX-containing preparations, varnishes, and a CHX gel applied on artificial fissures. CHX determination was conducted in a microplate reader using p...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-007-0166-4
更新日期:2008-09-01 00:00:00
abstract::The purpose of this study was to test the hypothesis of multidimensionality of oral and general health behaviour. To evaluate the proposed behavioural dimensions, relationships with individual beliefs and socio-economic factors were explored. A simple random sample of 1190 residents born in 1972 was drawn from the pop...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840000102
更新日期:2001-06-01 00:00:00
abstract:OBJECTIVES:Several theories have been proposed regarding the genesis of sialoliths, including the organic core theory, which suggests epithelial or bacterial etiology originating in the central core. Our aim was to use novel methodologies to analyze central areas (the core) of calculi from sialolithiasis patients. MAT...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-012-0870-6
更新日期:2013-09-01 00:00:00
abstract::The aim of this study was to compare detection of non-cavitated approximal caries lesions in images from seven solid-state intraoral digital receptors, with particular focus on two task-specific enhancement filters. One hundred and sixty approximal non-cavitated surfaces were radiographed under standardized conditions...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-007-0173-5
更新日期:2008-09-01 00:00:00
abstract:OBJECTIVES:Τhis study aims at determining the ability of cone beam computed tomography (CBCT) to visualize critical-size defects (CSD) created at rat calvaria and filled with 75/25 w/w nano-hydroxyapatite/chitosan (nHAp/CS) scaffolds, prior to their histological investigation. MATERIALS AND METHODS:Thirty adult Spragu...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02939-4
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:The aim of this comprehensive literature review is to provide recommendations and guidelines for dental implant therapy in patients with a history of radiation in the head and neck region. For the first time, a meta-analysis comparing the implant survival in irradiated and non-irradiated patients was perform...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00784-013-1134-9
更新日期:2014-04-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to evaluate the efficacy of four different side-to-side toothbrushes and the impact of various brushing parameters on noncontact biofilm removal in an adjustable interdental space model. MATERIALS AND METHODS:A three-species biofilm, consisting of Porphyromonas gingivalis, Fu...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1969-y
更新日期:2017-06-01 00:00:00
abstract::Oral verrucous carcinoma (OVC) is a rare variant of squamous cell carcinoma with a characteristic morphology and specific behavior. To date, few studies are available focusing the prevalence and clinicopathologic features of the oral verrucous carcinoma in Brazilian population. A total of 3,500 primary oral well-diffe...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-006-0050-7
更新日期:2006-09-01 00:00:00
abstract:OBJECTIVE:The aim of this in vitro study was to compare fluorescence-aided caries excavation with conventional excavation based on the Martens and Vickers hardness of dentin at the cavity floor after caries removal. MATERIALS AND METHODS:In total, 20 extracted human teeth with dentin caries were bisected through the l...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-0999-y
更新日期:2014-01-01 00:00:00
abstract::The purpose of this study was to determine the risk of microbiological transmission into the dental laboratory via impressions and dental stone models. Metal master models were contaminated with Bacillus subtilis and Streptococcus sanguis. Impressions in alginate, polyvinylsiloxane, and polyether were used, and models...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-002-0174-3
更新日期:2002-09-01 00:00:00
abstract::The aim of this study was to determine the prevalence of dental erosion in young Icelandic adults (19-22 years old) and patients with gastroesophageal reflux disease (GERD), in relation to their soft drink consumption and gastroesophageal reflux. Eighty subjects (40 males and 40 females), comprising 57 young adults (m...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-003-0252-1
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVE:A study is made of the main oral manifestations of patients with rheumatoid arthritis (RA), particularly salivary flow, and of its possible association to periodontal disease. MATERIAL AND METHODS:A prospective comparative study was made of 146 patients (73 with RA and 73 controls), recording pocket depth, c...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-016-1745-z
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the palatal total support tissues (TSTs) and bone support tissues (BSTs) at 5-mm paramedian section to the midsagittal suture between mouth breathers with high-narrow palates and nose breathers with normal palates and confirm the practicability and limitation on superimpo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-03004-w
更新日期:2020-03-01 00:00:00
abstract::We determined the effects of soluble or coated nanocrystalline hydroxyapatite paste (nano-HA) and enamel matrix derivative (EMD) on proliferation, adhesion, and migration of periodontal ligament fibroblasts (PDLs). Cultured PDLs were stimulated with nano-HA paste or EMD in a soluble form or were coated to the surface ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-011-0570-7
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:The objective of this study was to investigate the effects of fixed functional therapy on oropharyngeal airway dimensions and hyoid bone positions in Class II patients and make comparison with an untreated Class II group. MATERIALS AND METHODS:Eighteen patients (8 girls, 10 boys; mean age 13.62 ± 1.92 years)...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1659-1
更新日期:2016-09-01 00:00:00
abstract::This study evaluated by standardised digitised periapical radiography the crestal bone maintenance around modified diameter internal hex implants with variable thread design and narrow neck loaded with different procedures. Forty implants were placed in 25 patients. Twenty implants were conventionally loaded, 20 ones ...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-009-0314-0
更新日期:2010-08-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the chemokine expression profiles in gingival crevicular fluid (GCF) and serum in patients with advanced chronic periodontitis and to assess the impact of smoking on local and systemic levels of chemokines. MATERIALS AND METHODS:Thirty patients with chronic periodonti...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1314-2
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to compare the effects of a hemostatic collagen sponge and a collagen sponge sealed with a bio-adhesive material on the palatal donor sites with the purpose of minimizing postoperative pain after epithelialized gingival graft (EGG) harvesting. MATERIAL AND METHODS:The present study...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-018-2455-5
更新日期:2019-01-01 00:00:00
abstract::To evaluate the efficacy of metronidazole on cyclosporine-induced gingival overgrowth (GO), a prospective intra-subject double-blind longitudinal study was performed on six heart transplant patients with GO. All patients underwent scaling and root planing before any treatment. Metronidazole gel (Elyzol, Cabon) was the...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00784-001-0142-3
更新日期:2002-03-01 00:00:00
abstract:OBJECTIVES:Milling is a crucial step in producing restorations using computer-aided design and computer-aided manufacturing (CAD/CAM) systems. In this study the trueness of currently available milling devices was evaluated. MATERIALS AND METHODS:Thirty clinical cases (ten inlays, ten crowns, ten onlays) were milled fr...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1916-y
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVES:The aim of the meta-analysis was to clarify the efficacy of non-surgical periodontal treatment (NSPT) in improving rheumatoid arthritis (RA) disease activity. METHODS:A systematic literature search was conducted using the PubMed, Embase, and Cochrane databases up to October 2020. A total of nine studies wer...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-021-03807-w
更新日期:2021-01-29 00:00:00
abstract::Aim of this investigation was to evaluate the validity and reproducibility of the universal visual scoring systems (UniViSS) on occlusal surfaces in vitro. The validity study included 65 third molars. Following to reach a UniViSS consensus diagnosis for each surface, all teeth were histologically prepared and evaluate...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-010-0390-1
更新日期:2011-04-01 00:00:00
abstract:OBJECTIVES:Candida albicans is a common fungal infection and is commensal in 40-65 % of healthy adults. The development and pharmacokinetics of a novel sustained release clotrimazole varnish (Clot-SRV) for topical oral use have been reported. The aim of this study was to compare the efficacy of this varnish with clotri...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1259-5
更新日期:2015-03-01 00:00:00
abstract::This study evaluated the surface roughness change and wear provided by different dentifrices on root dentine previously exposed to erosive challenges. According to a randomized complete block design, 150 slabs of bovine root dentine (6 x 3 x 2 mm) were ground flat and polished. In an area of 4 x 3 mm on the dentine su...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-004-0262-7
更新日期:2004-09-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to quantify the symmetry of the alveolar process of the maxilla and palate during the first year of life in healthy infants with the help of a semiautomatic segmentation technique. MATERIALS AND METHODS:Maxillary plaster models of seventy healthy babies at 0, 3, 6, 9, and 12 mo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03403-4
更新日期:2020-06-24 00:00:00
abstract:OBJECTIVES:The combination of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) yields a "precipitate potentially toxic" (PPT). The aim of this study was to evaluate the tissue response to implanted polyethylene tubes filled with PPT-soaked fibrin sponge. METHODS:Forty rats received four polyethylene tubes each; eac...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1049-5
更新日期:2014-04-01 00:00:00
abstract::Regular screening through white light inspection of the entire oral mucosa is the most important examination method to identify precancerous lesions and early oral carcinoma. Additionally, the physiologic autofluorescence of the oral mucosa has been described as a novel screening method for the detection of mucosal le...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章
doi:10.1007/s00784-010-0455-1
更新日期:2011-12-01 00:00:00
abstract:OBJECTIVES:To date, scarce evidence exists around the application of subgingival air polishing during treatment of severe periodontitis. The aim of this study was to evaluate the benefits of subgingival air polishing during non-surgical treatment of deep bleeding pockets in stages III-IV periodontitis patients MATERIAL...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-020-03648-z
更新日期:2021-02-01 00:00:00