Abstract：
:We formerly developed an automatic colony count system based on the time-lapse shadow image analysis (TSIA). Here this system has been upgraded and applied to practical rapid decision. A microbial sample was spread on/in an agar plate with 90 mm in diameter as homogeneously as possible. We could obtain the results with several strains that most of colonies appeared within a limited time span. Consequently the number of colonies reached a steady level (Nstdy) and then unchanged until the end of long culture time to give the confirmed value (Nconf). The equivalence of Nstdy and Nconf as well as the difference of times for Nstdy and Nconf determinations were statistically significant at p < 0.001. Nstdy meets the requirement of practical routines treating a large number of plates. The difference of Nstdy and Nconf, if any, may be elucidated by means of retrievable big data. Therefore Nconf is valid for official documentation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ogawa H,Nasu S,Takeshige M,Saito M,Matsuoka Hdoi
10.1038/srep10061subject
Has Abstractpub_date
2015-05-15 00:00:00pages
10061issn
2045-2322pii
srep10061journal_volume
5pub_type
杂志文章abstract：:We compared commercial kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing. Purification of DNA was based on matrix binding (silica or anion exchange resin) or differential precipitation (salting out), respectively. The choice of extraction kit had ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28063
更新日期：2016-06-17 00:00:00
abstract：:The Edinburgh Postnatal Depression Scale (EPDS) is frequently used to screen for postpartum depression. However, its factor structure exhibits noticeable inconsistencies between studies. We examined the EPDS at two postpartum time points using a large dataset from outside Western countries. Participants were 91,063 mo...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章,多中心研究
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更新日期：2020-07-15 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
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更新日期：2018-07-03 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20374-5
更新日期：2018-02-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
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更新日期：2018-03-12 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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abstract：:Type I interferons, particularly interferon-alpha (IFN-α), play a vital role in the host's anti-viral defenses by interfering with viral replication. However, the virus rapidly evolves to exploit the IFN-α response for its replication, spread, and pathogenic function. In this study, we attempted to determine IFN-α sus...
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pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-04-24 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-02-11 00:00:00
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更新日期：2019-11-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2017-09-08 00:00:00
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journal_title：Scientific reports
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journal_title：Scientific reports
pub_type： 杂志文章
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journal_title：Scientific reports
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更新日期：2016-02-03 00:00:00
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更新日期：2018-11-01 00:00:00
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