Abstract：
:Pluripotent stem cells (PSCs) have various degrees of pluripotency, which necessitates selection of PSCs with high pluripotency before their application to regenerative medicine. However, the quality control processes for PSCs are costly and time-consuming, and it is essential to develop inexpensive and less laborious selection methods for translation of PSCs into clinical applications. Here we developed an imaging system, termed Phase Distribution (PD) imaging system, which visualizes subcellular structures quantitatively in unstained and unlabeled cells. The PD image and its derived PD index reflected the mitochondrial content, enabling quantitative evaluation of the degrees of somatic cell reprogramming and PSC differentiation. Moreover, the PD index allowed unbiased grouping of PSC colonies into those with high or low pluripotency without the aid of invasive methods. Finally, the PD imaging system produced three-dimensional images of PSC colonies, providing further criteria to evaluate pluripotency of PSCs. Thus, the PD imaging system may be utilized for screening of live PSCs with potentially high pluripotency prior to more rigorous quality control processes.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nishimura K,Ishiwata H,Sakuragi Y,Hayashi Y,Fukuda A,Hisatake Kdoi
10.1038/s41598-018-37779-xsubject
Has Abstractpub_date
2019-02-11 00:00:00pages
1777issue
1issn
2045-2322pii
10.1038/s41598-018-37779-xjournal_volume
9pub_type
杂志文章abstract：:Neuroblastoma is the most commonly diagnosed extracranial tumor in the first year of life. Approximately 9% of neuroblastoma patients present germline or somatic aberrations in the gene encoding for anaplastic lymphoma kinase (ALK). This increases in high-risk neuroblastomas, which have a 14% frequency of ALK aberrati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55060-7
更新日期：2019-12-18 00:00:00
abstract：:In plants, pathogen triggered programmed cell death (PCD) is frequently mediated by polar lipid molecules referred as long chain bases (LCBs) or ceramides. PCD interceded by LCBs is a well-organized process where several cell organelles play important roles. In fact, light-dependent reactions in the chloroplast have b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65186-8
更新日期：2020-06-25 00:00:00
abstract：:Mechanistic target of rapamycin (mTOR) is a serine/threonine protein kinase that mediates phosphoinositide-3-kinase (PI3K)/AKT signalling. This pathway is involved in a plethora of cellular functions including protein and lipid synthesis, cell migration, cell proliferation and apoptosis. In this study, we proposed to ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53141-1
更新日期：2019-11-15 00:00:00
abstract：:DNA double-strand breaks pose a direct threat to genomic stability. Studies of DNA damage and chromatin dynamics have yielded opposing results that support either increased or decreased chromatin motion after damage. In this study, we independently measure the dynamics of transcriptionally active or repressed chromati...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36343-x
更新日期：2018-12-27 00:00:00
abstract：:Bronchoconstrictive airway disorders such as asthma are characterized by inflammation and increases in reactive oxygen species (ROS), which produce a highly oxidative environment. β2-adrenergic receptor (β2AR) agonists are a mainstay of clinical therapy for asthma and provide bronchorelaxation upon inhalation. We have...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-59983-4
更新日期：2020-02-19 00:00:00
abstract：:Exploration of macroscopic quantum entanglement is of great interest in both fundamental science and practical application. We investigate a hybrid quantum system that consists of two nitrogen-vacancy centers ensembles (NVE) coupled to a superconducting coplanar waveguide resonator (CPWR). The collective magnetic coup...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep21775
更新日期：2016-02-23 00:00:00
abstract：:Two polar vessels, Akademik Shokalskiy and Xuelong, were trapped by thick sea ice in the Antarctic coastal region just to the west of 144°E and between 66.5°S and 67°S in late December 2013. This event demonstrated the rapid establishment of extreme Antarctic sea ice conditions on synoptic time scales. The event was a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05317
更新日期：2014-06-17 00:00:00
abstract：:Ancestral environmental exposures to a variety of factors and toxicants have been shown to promote the epigenetic transgenerational inheritance of adult onset disease. One of the most widely used agricultural pesticides worldwide is the herbicide glyphosate (N-(phosphonomethyl)glycine), commonly known as Roundup. Ther...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42860-0
更新日期：2019-04-23 00:00:00
abstract：:Developing optically transparent magnets at room temperature is an important challenge. They would bring many innovations to various industries, not only for electronic and magnetic devices but also for optical applications. Here we introduce FeCo-(Al-fluoride) nanogranular films exhibiting ferromagnetic properties wi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34227
更新日期：2016-09-28 00:00:00
abstract：:Ginseng rusty root (GRR) symptom is one of the primary diseases of ginseng. There has been a problem of ginseng rusty root, leading to a severe decline in the quality of ginseng. To clarify the relationship between root symptoms of ginseng rust and soil, the physical and chemical properties, enzyme activity, community...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71024-8
更新日期：2020-09-25 00:00:00
abstract：:Learning to successfully navigate social environments is a critical developmental goal, predictive of long-term wellbeing. However, little is known about how people learn to adjust to different social environments, and how this behaviour emerges across development. Here, we use a series of economic games to assess how...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78546-1
更新日期：2020-12-10 00:00:00
abstract：:An ultra-fast physical random number generator is demonstrated utilizing a photonic integrated device based broadband chaotic source with a simple post data processing method. The compact chaotic source is implemented by using a monolithic integrated dual-mode amplified feedback laser (AFL) with self-injection, where ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45900
更新日期：2017-04-04 00:00:00
abstract：:Cardiac tissue engineering using biomaterials with or without combination of stem cell therapy offers a new option for repairing infarcted heart. However, the bioactivity of biomaterials remains to be optimized because currently available biomaterials do not mimic the biochemical components as well as the structural p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39988
更新日期：2017-01-04 00:00:00
abstract：:Fluid-feeding insects have evolved a unique strategy to distribute the labor between a liquid-acquisition device (proboscis) and a sucking pump. We theoretically examined physical constraints associated with coupling of the proboscis and sucking pump into a united functional organ. Classification of fluid feeders with...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06391-w
更新日期：2017-07-26 00:00:00
abstract：:The epigenetic status of the genome changes dynamically from fertilization to implantation. In addition, the physiological environment during the process of gametogenesis, including parental age, may affect the epigenome of the embryo after fertilization. It is important to clarify the influence of parental age on gen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20614-8
更新日期：2018-02-05 00:00:00
abstract：:Peripheral artery disease (PAD) is a major cause of acute and chronic illness, with extremely poor prognosis that remains underdiagnosed and undertreated. Trimethylamine-N-Oxide (TMAO), a gut derived metabolite, has been associated with atherosclerotic burden. We determined plasma levels of TMAO by mass spectrometry a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52082-z
更新日期：2019-10-30 00:00:00
abstract：:Somatic embryogenesis receptor kinases (SERKs) belong to a small gene family of receptor-like kinases involved in signal transduction. A total of 54 genes were shortlisted from the wheat genome survey sequence of which 5 were classified as SERKs and 49 were identified as SERK-like (SERLs). Tissue- specific expression ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10038-1
更新日期：2017-09-28 00:00:00
abstract：:The large and complex genome of Pseudomonas aeruginosa, which consists of significant portions (up to 20%) of transferable genetic elements contributes to the rapid development of antibiotic resistance. The whole genome sequences of 22 strains isolated from eye and cystic fibrosis patients in Australia and India betwe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34020-7
更新日期：2018-10-23 00:00:00
abstract：:In this work, surface plasmons (SPs) on a germanium (Ge) thin film in terahertz (THz) region that are excited by electron cyclotron motion (ECM) and the subsequent SP emission (SPE) by adding Ge gratings on the film are explored by finite-difference time-domain (FDTD) and particle-in-cell FDTD (PIC-FDTD) simulations. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73725-6
更新日期：2020-10-07 00:00:00
abstract：:Oxaliplatin, a chemotherapy medication, causes severe peripheral neuropathy. Although oxaliplatin-induced peripheral neuropathy is a dose-limiting toxicity, a therapeutic strategy against its effects has not been established. We previously reported the involvement of N-methyl-D-aspartate receptors and their intracellu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08891-1
更新日期：2017-08-29 00:00:00
abstract：:Magnetic nanoparticles dissipate heat when exposed to alternating magnetic fields (AMFs), making them suitable for cancer hyperthermia. Therapeutic heating applications demand accurate characterization of the heating power dissipated by the particles. Specific loss power (SLP) generated by magnetic nanoparticles is es...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07088-w
更新日期：2017-07-27 00:00:00
abstract：:Arctic amplification of global warming has led to increased summer sea ice retreat, which influences gas exchange between the Arctic Ocean and the atmosphere where sea ice previously acted as a physical barrier. Indeed, recently observed enhanced atmospheric methane concentrations in Arctic regions with fractional sea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16179
更新日期：2015-11-10 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-57850-w
更新日期：2020-01-21 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-23329-y
更新日期：2018-03-19 00:00:00
abstract：:Radiotherapy (RT) is one of the most frequently used methods for cancer treatment. Despite remarkable advancements in RT techniquesthe treatment of radioresistant tumours (i.e. high-grade gliomas) is not yet satisfactory. Finding novel approaches less damaging for normal tissues is of utmost importance. This would mak...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70371-w
更新日期：2020-08-11 00:00:00
abstract：:Culturomics is a high-throughput culture approach that has dramatically contributed to the recent renewal of culture. While metagenomics enabled substantial advances in exploring the microbiota, culturomics significantly expanded our knowledge regarding the bacterial gut repertoire through the discovery and the descri...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66738-8
更新日期：2020-06-15 00:00:00
abstract：:CDK5 Regulatory Subunit-Associated Protein 1-like 1 (CDKAL1) was identified as a susceptibility gene for type 2 diabetes and body mass index in genome-wide association studies. Although it was reported that CDKAL1 is a methylthiotransferase essential for tRNALys(UUU) and faithful translation of proinsulin generated in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06469-5
更新日期：2017-08-04 00:00:00
abstract：:The ability to obtain three-dimensional (3-D) information about morphologies of nanostructures elucidates many interesting properties of materials in both physical and biological sciences. Here we demonstrate a novel method in scanning transmission electron microscopy (STEM) that gives a fast and reliable assessment o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07537-6
更新日期：2017-09-06 00:00:00
abstract：:A key challenge of central nervous system tumor surgery is to discriminate between brain regions infiltrated by tumor cells and surrounding healthy tissue. Although monitoring of autofluorescence could potentially be an efficient way to provide reliable information for these regions, we found little information on thi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14381-1
更新日期：2017-10-25 00:00:00
abstract：:The recent outbreaks of the insect-vectored Zika virus have demonstrated its potential to be sexually transmitted, which complicates modeling and our understanding of disease dynamics. Autochthonous outbreaks in the US mainland may be a consequence of both modes of transmission, which affect the outbreak size, duratio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43651-3
更新日期：2019-05-10 00:00:00
